Mutagenetix > Incidental Mutation

Incidental Mutation 'R3788:Or7e177'

272385

Institutional Source

Beutler Lab

Gene Symbol

Or7e177

Ensembl Gene

ENSMUSG00000049028

Gene Name

olfactory receptor family 7 subfamily E member 177

Synonyms

Olfr873, GA_x6K02T2PVTD-14040245-14041204, MOR145-2

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.097) question?

Stock #

R3788 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

20211507-20212466 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 20211666 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 58 (I58V)

Ref Sequence

ENSEMBL: ENSMUSP00000075135 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053919] [ENSMUST00000075717] [ENSMUST00000215540]

AlphaFold

E9PX82

Predicted Effect

probably benign
Transcript: ENSMUST00000053919
AA Change: I54V

PolyPhen 2 Score 0.017 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000054778
Gene: ENSMUSG00000049028
AA Change: I54V

Domain	Start	End	E-Value	Type
Pfam:7tm_4	41	317	1.7e-52	PFAM
Pfam:7TM_GPCR_Srsx	45	315	1.6e-8	PFAM
Pfam:7tm_1	51	300	3e-21	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000075717
AA Change: I58V

PolyPhen 2 Score 0.129 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000075135
Gene: ENSMUSG00000049028
AA Change: I58V

Domain	Start	End	E-Value	Type
Pfam:7tm_4	45	321	6.2e-43	PFAM
Pfam:7TM_GPCR_Srsx	49	309	3e-8	PFAM
Pfam:7tm_1	55	304	1.5e-20	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000212393

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000212793

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000213057

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000213445

Predicted Effect

probably benign
Transcript: ENSMUST00000215540
AA Change: I57V

PolyPhen 2 Score 0.035 (Sensitivity: 0.94; Specificity: 0.82)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000217193

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000216567

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.1%

Validation Efficiency

100% (61/61)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 61 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca4	T	A	3: 121,846,561 (GRCm39)	V26E	possibly damaging	Het
Abhd16a	A	G	17: 35,320,563 (GRCm39)	N411S	probably damaging	Het
Akap13	G	A	7: 75,351,901 (GRCm39)		probably null	Het
Aph1b	T	C	9: 66,701,348 (GRCm39)		probably benign	Het
Aspm	C	T	1: 139,390,941 (GRCm39)	T742I	probably damaging	Het
Bclaf3	A	G	X: 158,349,492 (GRCm39)	H619R	probably benign	Het
Bltp2	T	A	11: 78,179,123 (GRCm39)		probably null	Het
Cemip	A	T	7: 83,593,106 (GRCm39)	L1199H	probably damaging	Het
Chd2	G	A	7: 73,096,878 (GRCm39)		probably benign	Het
Clnk	A	G	5: 38,872,341 (GRCm39)	Y310H	probably damaging	Het
Crmp1	A	G	5: 37,441,484 (GRCm39)	D522G	probably damaging	Het
Cyth3	A	G	5: 143,622,298 (GRCm39)		probably benign	Het
Dcbld1	T	A	10: 52,195,754 (GRCm39)	Y392N	probably damaging	Het
Flnc	T	C	6: 29,454,056 (GRCm39)	F1820L	probably damaging	Het
Galnt18	G	A	7: 111,119,322 (GRCm39)	R385*	probably null	Het
Gpatch3	C	A	4: 133,302,479 (GRCm39)	R137S	possibly damaging	Het
Gpc6	C	T	14: 117,861,878 (GRCm39)	P265S	probably damaging	Het
Harbi1	T	A	2: 91,550,952 (GRCm39)	D308E	probably benign	Het
Hdhd2	G	A	18: 77,042,883 (GRCm39)		probably null	Het
Hk1	T	C	10: 62,111,467 (GRCm39)	K737E	possibly damaging	Het
Hnrnpr	G	A	4: 136,063,624 (GRCm39)	V345M	probably damaging	Het
Ift56	T	A	6: 38,380,459 (GRCm39)		probably null	Het
Kalrn	T	C	16: 34,040,610 (GRCm39)	H944R	probably damaging	Het
Kdm2a	A	T	19: 4,401,833 (GRCm39)	C207S	probably damaging	Het
Kirrel1	C	T	3: 86,996,458 (GRCm39)	M380I	probably null	Het
Krt75	C	T	15: 101,481,956 (GRCm39)	G104D	possibly damaging	Het
Lnpk	A	T	2: 74,352,607 (GRCm39)	S358R	probably benign	Het
Map2	A	G	1: 66,456,022 (GRCm39)	T1512A	probably damaging	Het
Marchf10	T	A	11: 105,287,905 (GRCm39)	L132F	probably damaging	Het
Mfrp	G	A	9: 44,016,754 (GRCm39)	W65*	probably null	Het
Mgat5	A	G	1: 127,294,180 (GRCm39)	D174G	probably benign	Het
Miga2	T	A	2: 30,261,237 (GRCm39)	Y177*	probably null	Het
Mroh3	T	C	1: 136,113,213 (GRCm39)	D747G	probably damaging	Het
Muc5b	A	G	7: 141,417,571 (GRCm39)	T3506A	possibly damaging	Het
Myo7b	G	T	18: 32,107,165 (GRCm39)	P1277T	possibly damaging	Het
Naaa	C	T	5: 92,420,413 (GRCm39)		probably null	Het
Ndufs2	T	C	1: 171,062,889 (GRCm39)	D410G	possibly damaging	Het
Or51a25	A	G	7: 102,372,694 (GRCm39)		probably null	Het
Or5p78	T	A	7: 108,212,280 (GRCm39)	Y255*	probably null	Het
Or8g35	A	G	9: 39,381,365 (GRCm39)	I219T	probably benign	Het
Osbp	A	T	19: 11,956,285 (GRCm39)	Y409F	probably benign	Het
Plxnb1	T	A	9: 108,938,355 (GRCm39)	V1303D	possibly damaging	Het
Prkcg	G	A	7: 3,362,263 (GRCm39)	D246N	probably damaging	Het
Ranbp17	GCCTGGATACTGACC	GCC	11: 33,169,203 (GRCm39)		probably benign	Het
Sbf1	G	A	15: 89,183,731 (GRCm39)	R1261*	probably null	Het
Scn4a	T	C	11: 106,235,100 (GRCm39)	N341S	probably damaging	Het
Sec61a2	C	A	2: 5,884,436 (GRCm39)		probably null	Het
Sgcd	T	A	11: 47,246,032 (GRCm39)	K57*	probably null	Het
Sinhcaf	A	G	6: 148,827,617 (GRCm39)	S134P	possibly damaging	Het
Slc12a5	T	C	2: 164,835,695 (GRCm39)	L861P	probably damaging	Het
Slc6a16	A	G	7: 44,909,386 (GRCm39)	D184G	probably benign	Het
Snx7	A	G	3: 117,632,639 (GRCm39)		probably benign	Het
Sptbn2	A	G	19: 4,795,950 (GRCm39)	I1710V	probably damaging	Het
Sytl2	A	T	7: 90,025,289 (GRCm39)	I426F	probably benign	Het
Tdp1	A	G	12: 99,858,011 (GRCm39)		probably benign	Het
Tmem232	C	A	17: 65,689,628 (GRCm39)	D496Y	possibly damaging	Het
Tomm20l	C	T	12: 71,158,516 (GRCm39)	A58V	possibly damaging	Het
Ttn	T	C	2: 76,775,618 (GRCm39)	E1854G	unknown	Het
Ttn	A	G	2: 76,804,552 (GRCm39)	V240A	probably benign	Het
Vmn2r98	A	T	17: 19,300,887 (GRCm39)	T630S	probably benign	Het
Xrcc1	G	C	7: 24,266,333 (GRCm39)	A220P	probably benign	Het

Other mutations in Or7e177

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02122:Or7e177	APN	9	20,211,880 (GRCm39)	missense	probably damaging	1.00
IGL02268:Or7e177	APN	9	20,211,588 (GRCm39)	missense	probably damaging	1.00
IGL02416:Or7e177	APN	9	20,211,541 (GRCm39)	missense	probably benign	0.01
IGL03124:Or7e177	APN	9	20,212,459 (GRCm39)	missense	probably benign	0.00
R0147:Or7e177	UTSW	9	20,212,387 (GRCm39)	missense	probably damaging	1.00
R0148:Or7e177	UTSW	9	20,212,387 (GRCm39)	missense	probably damaging	1.00
R0266:Or7e177	UTSW	9	20,212,454 (GRCm39)	missense	probably benign	0.01
R0831:Or7e177	UTSW	9	20,211,861 (GRCm39)	missense	probably benign	0.20
R1456:Or7e177	UTSW	9	20,212,134 (GRCm39)	missense	probably benign	0.35
R1894:Or7e177	UTSW	9	20,211,633 (GRCm39)	missense	probably benign	0.23
R1928:Or7e177	UTSW	9	20,212,354 (GRCm39)	missense	probably benign	0.12
R2135:Or7e177	UTSW	9	20,211,593 (GRCm39)	missense	probably benign	0.00
R2379:Or7e177	UTSW	9	20,211,963 (GRCm39)	missense	possibly damaging	0.87
R2911:Or7e177	UTSW	9	20,211,775 (GRCm39)	missense	possibly damaging	0.60
R4657:Or7e177	UTSW	9	20,211,919 (GRCm39)	missense	probably damaging	1.00
R5754:Or7e177	UTSW	9	20,212,390 (GRCm39)	missense	probably damaging	1.00
R6291:Or7e177	UTSW	9	20,211,899 (GRCm39)	missense	probably damaging	1.00
R6410:Or7e177	UTSW	9	20,211,748 (GRCm39)	missense	probably damaging	1.00
R7014:Or7e177	UTSW	9	20,211,959 (GRCm39)	nonsense	probably null
R7521:Or7e177	UTSW	9	20,212,036 (GRCm39)	missense	probably benign	0.00
R8201:Or7e177	UTSW	9	20,212,317 (GRCm39)	missense	probably damaging	1.00
R8355:Or7e177	UTSW	9	20,211,715 (GRCm39)	missense	probably damaging	1.00
R8455:Or7e177	UTSW	9	20,211,715 (GRCm39)	missense	probably damaging	1.00
R8523:Or7e177	UTSW	9	20,212,093 (GRCm39)	missense	probably benign	0.10
R8874:Or7e177	UTSW	9	20,212,069 (GRCm39)	missense	possibly damaging	0.95
R9283:Or7e177	UTSW	9	20,212,419 (GRCm39)	missense	possibly damaging	0.61
R9397:Or7e177	UTSW	9	20,211,748 (GRCm39)	missense	possibly damaging	0.50
R9595:Or7e177	UTSW	9	20,211,661 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAGTGTCGAGTGATAGTAAACTTC -3'
(R):5'- GGCCATCACAGTCAGAAGCAAG -3'

Sequencing Primer
(F):5'- GTCTCAGAATTCCATCTCATG -3'
(R):5'- AAAAATGACATCTGTGTAAGGCATCC -3'

Posted On

2015-03-25