Mutagenetix > Incidental Mutation

Incidental Mutation 'R3799:Vmn2r76'

272881

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r76

Ensembl Gene

ENSMUSG00000091239

Gene Name

vomeronasal 2, receptor 76

Synonyms

MMRRC Submission

040877-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.134) question?

Stock #

R3799 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

85874414-85895409 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 85875244 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 578 (T578A)

Ref Sequence

ENSEMBL: ENSMUSP00000127309 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165771]

AlphaFold

E9Q3F5

Predicted Effect

probably benign
Transcript: ENSMUST00000165771
AA Change: T578A

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000127309
Gene: ENSMUSG00000091239
AA Change: T578A

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	78	470	1.2e-29	PFAM
low complexity region	476	489	N/A	INTRINSIC
Pfam:NCD3G	513	565	3.7e-22	PFAM
Pfam:7tm_3	598	833	1.4e-52	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.1%

Validation Efficiency

97% (35/36)

Allele List at MGI

Other mutations in this stock

Total: 33 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A3galt2	A	G	4: 128,660,863 (GRCm39)	T140A	probably damaging	Het
Adgrf3	T	C	5: 30,401,821 (GRCm39)	I736V	possibly damaging	Het
Alpk3	C	A	7: 80,742,501 (GRCm39)	P773T	probably benign	Het
Ccna1	G	T	3: 54,958,040 (GRCm39)	T155K	probably benign	Het
Cops7a	C	T	6: 124,936,795 (GRCm39)	R252H	probably damaging	Het
Dnah12	T	C	14: 26,492,880 (GRCm39)	W1214R	probably damaging	Het
Ehbp1l1	A	G	19: 5,769,143 (GRCm39)	V720A	probably benign	Het
Exog	T	G	9: 119,278,876 (GRCm39)	N186K	probably damaging	Het
Fgfr1	G	A	8: 26,062,453 (GRCm39)	D663N	probably damaging	Het
Flnc	G	A	6: 29,443,738 (GRCm39)	V587M	probably damaging	Het
Gm1527	A	G	3: 28,980,745 (GRCm39)	N615S	possibly damaging	Het
Gm5799	G	A	14: 43,781,150 (GRCm39)	G17E	probably damaging	Het
Hjurp	G	C	1: 88,204,937 (GRCm39)		probably benign	Het
Mastl	T	C	2: 23,030,504 (GRCm39)		probably benign	Het
Mogat1	A	G	1: 78,505,775 (GRCm39)	I216V	probably benign	Het
Nans	A	G	4: 46,492,839 (GRCm39)	E89G	probably benign	Het
Nuggc	T	A	14: 65,857,087 (GRCm39)	M396K	probably benign	Het
Nup214	T	C	2: 31,924,694 (GRCm39)	F236S	probably damaging	Het
Osbp2	T	C	11: 3,667,883 (GRCm39)	E145G	probably damaging	Het
Paqr3	T	C	5: 97,259,175 (GRCm39)	N43S	probably damaging	Het
Pard3b	A	G	1: 62,200,388 (GRCm39)	N309S	probably benign	Het
Phrf1	C	T	7: 140,839,831 (GRCm39)	R243*	probably null	Het
Ralgapa1	T	C	12: 55,705,915 (GRCm39)	Y1869C	probably damaging	Het
Setd2	T	C	9: 110,378,639 (GRCm39)	V818A	probably benign	Het
Slc35g3	A	G	11: 69,651,743 (GRCm39)	F103L	probably benign	Het
Tcaf3	T	C	6: 42,574,014 (GRCm39)	E66G	probably damaging	Het
Tdpoz8	A	T	3: 92,981,393 (GRCm39)	D137V	probably damaging	Het
Tmem8b	A	G	4: 43,673,892 (GRCm39)		probably benign	Het
Trpa1	T	C	1: 14,963,488 (GRCm39)	N578S	possibly damaging	Het
Vmn2r25	A	T	6: 123,830,143 (GRCm39)	L3I	probably benign	Het
Vwa8	T	A	14: 79,302,336 (GRCm39)	F1002I	probably damaging	Het
Xdh	T	C	17: 74,214,653 (GRCm39)	E764G	probably damaging	Het
Zfp518a	G	T	19: 40,903,754 (GRCm39)	V1228F	probably damaging	Het

Other mutations in Vmn2r76

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00969:Vmn2r76	APN	7	85,877,925 (GRCm39)	missense	probably benign
IGL01374:Vmn2r76	APN	7	85,874,857 (GRCm39)	missense	probably benign	0.02
IGL01419:Vmn2r76	APN	7	85,874,910 (GRCm39)	missense	probably benign	0.32
IGL01627:Vmn2r76	APN	7	85,874,871 (GRCm39)	missense	probably damaging	1.00
IGL01730:Vmn2r76	APN	7	85,879,406 (GRCm39)	missense	probably benign	0.02
IGL01957:Vmn2r76	APN	7	85,877,925 (GRCm39)	missense	probably benign
IGL02214:Vmn2r76	APN	7	85,879,138 (GRCm39)	missense	probably benign	0.07
IGL02489:Vmn2r76	APN	7	85,878,071 (GRCm39)	missense	probably benign	0.41
IGL02543:Vmn2r76	APN	7	85,879,356 (GRCm39)	missense	probably benign	0.06
IGL02579:Vmn2r76	APN	7	85,877,961 (GRCm39)	nonsense	probably null
IGL02598:Vmn2r76	APN	7	85,877,879 (GRCm39)	missense	probably benign	0.05
IGL02720:Vmn2r76	APN	7	85,874,914 (GRCm39)	missense	probably benign	0.35
IGL02745:Vmn2r76	APN	7	85,879,495 (GRCm39)	missense	probably benign	0.06
IGL03393:Vmn2r76	APN	7	85,879,034 (GRCm39)	missense	probably benign	0.01
R0483:Vmn2r76	UTSW	7	85,874,959 (GRCm39)	missense	probably damaging	1.00
R0513:Vmn2r76	UTSW	7	85,877,987 (GRCm39)	missense	probably benign	0.01
R0528:Vmn2r76	UTSW	7	85,879,506 (GRCm39)	missense	possibly damaging	0.80
R0601:Vmn2r76	UTSW	7	85,875,323 (GRCm39)	critical splice acceptor site	probably null
R0662:Vmn2r76	UTSW	7	85,879,578 (GRCm39)	missense	probably benign	0.39
R0883:Vmn2r76	UTSW	7	85,877,904 (GRCm39)	missense	probably benign	0.00
R1532:Vmn2r76	UTSW	7	85,879,454 (GRCm39)	missense	probably benign	0.02
R1694:Vmn2r76	UTSW	7	85,879,356 (GRCm39)	missense	probably benign	0.06
R1696:Vmn2r76	UTSW	7	85,880,464 (GRCm39)	missense	possibly damaging	0.56
R2135:Vmn2r76	UTSW	7	85,880,219 (GRCm39)	missense	probably benign	0.02
R2151:Vmn2r76	UTSW	7	85,879,692 (GRCm39)	missense	probably benign
R2181:Vmn2r76	UTSW	7	85,874,743 (GRCm39)	missense	probably benign	0.00
R2268:Vmn2r76	UTSW	7	85,879,707 (GRCm39)	missense	probably benign	0.03
R2877:Vmn2r76	UTSW	7	85,875,201 (GRCm39)	missense	probably benign	0.00
R3155:Vmn2r76	UTSW	7	85,874,959 (GRCm39)	missense	probably damaging	1.00
R3746:Vmn2r76	UTSW	7	85,874,763 (GRCm39)	missense	probably benign	0.11
R3825:Vmn2r76	UTSW	7	85,880,415 (GRCm39)	missense	probably benign	0.10
R4058:Vmn2r76	UTSW	7	85,879,508 (GRCm39)	missense	probably benign	0.00
R4237:Vmn2r76	UTSW	7	85,879,740 (GRCm39)	missense	probably benign	0.00
R4404:Vmn2r76	UTSW	7	85,877,511 (GRCm39)	missense	probably benign	0.16
R4796:Vmn2r76	UTSW	7	85,879,652 (GRCm39)	missense	possibly damaging	0.95
R4838:Vmn2r76	UTSW	7	85,874,733 (GRCm39)	missense	probably damaging	1.00
R5175:Vmn2r76	UTSW	7	85,877,915 (GRCm39)	missense	probably benign	0.00
R5268:Vmn2r76	UTSW	7	85,875,267 (GRCm39)	missense	probably damaging	1.00
R5381:Vmn2r76	UTSW	7	85,874,496 (GRCm39)	missense	probably damaging	1.00
R5531:Vmn2r76	UTSW	7	85,874,657 (GRCm39)	missense	probably damaging	1.00
R5566:Vmn2r76	UTSW	7	85,875,286 (GRCm39)	missense	probably damaging	1.00
R5646:Vmn2r76	UTSW	7	85,875,261 (GRCm39)	missense	probably damaging	0.98
R5664:Vmn2r76	UTSW	7	85,895,202 (GRCm39)	critical splice donor site	probably null
R5818:Vmn2r76	UTSW	7	85,879,142 (GRCm39)	missense	probably benign	0.00
R6093:Vmn2r76	UTSW	7	85,877,469 (GRCm39)	nonsense	probably null
R6651:Vmn2r76	UTSW	7	85,878,059 (GRCm39)	missense	possibly damaging	0.64
R6741:Vmn2r76	UTSW	7	85,879,560 (GRCm39)	missense	probably benign
R6750:Vmn2r76	UTSW	7	85,875,114 (GRCm39)	missense	probably damaging	1.00
R7082:Vmn2r76	UTSW	7	85,874,440 (GRCm39)	missense	probably benign	0.01
R7136:Vmn2r76	UTSW	7	85,877,975 (GRCm39)	missense	probably benign	0.06
R7524:Vmn2r76	UTSW	7	85,879,374 (GRCm39)	missense	probably benign	0.00
R7524:Vmn2r76	UTSW	7	85,874,577 (GRCm39)	missense	probably benign	0.22
R7611:Vmn2r76	UTSW	7	85,879,388 (GRCm39)	missense	probably benign	0.04
R7833:Vmn2r76	UTSW	7	85,877,892 (GRCm39)	missense	probably benign
R8002:Vmn2r76	UTSW	7	85,879,271 (GRCm39)	missense	probably benign	0.05
R8021:Vmn2r76	UTSW	7	85,874,958 (GRCm39)	missense	probably damaging	1.00
R8023:Vmn2r76	UTSW	7	85,879,028 (GRCm39)	missense	probably benign	0.00
R8250:Vmn2r76	UTSW	7	85,875,231 (GRCm39)	missense	possibly damaging	0.82
R8428:Vmn2r76	UTSW	7	85,874,479 (GRCm39)	missense	possibly damaging	0.63
R8874:Vmn2r76	UTSW	7	85,877,999 (GRCm39)	missense	probably damaging	1.00
R9139:Vmn2r76	UTSW	7	85,879,170 (GRCm39)	missense	probably benign	0.02
R9357:Vmn2r76	UTSW	7	85,880,428 (GRCm39)	missense	probably benign	0.43
Z1176:Vmn2r76	UTSW	7	85,895,271 (GRCm39)	missense	probably benign	0.39

Predicted Primers

PCR Primer
(F):5'- GTGGTTGTCCAATGAATAGCAGAG -3'
(R):5'- GCTTCCATAAGAAAGGTCAATTTGG -3'

Sequencing Primer
(F):5'- TGTCCAATGAATAGCAGAGAACAAAG -3'
(R):5'- CCTGGTGTATAGATAAATCTTTG -3'

Posted On

2015-03-25