Mutagenetix > Incidental Mutations

Incidental Mutation 'R3801:Nrap'

273026

Institutional Source

Beutler Lab

Gene Symbol

Nrap

Ensembl Gene

ENSMUSG00000049134

Gene Name

nebulin-related anchoring protein

Synonyms

MMRRC Submission

040760-MU

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3801 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

56320035-56390037 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 56321779 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Asparagine at position 1595 (D1595N)

Ref Sequence

ENSEMBL: ENSMUSP00000132582 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000040711] [ENSMUST00000073536] [ENSMUST00000078284] [ENSMUST00000095947] [ENSMUST00000095948] [ENSMUST00000165522] [ENSMUST00000166049] [ENSMUST00000166203] [ENSMUST00000167239]

AlphaFold

Q80XB4

Predicted Effect

probably damaging
Transcript: ENSMUST00000040711
AA Change: D1596N

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000048364
Gene: ENSMUSG00000049134
AA Change: D1596N

Domain	Start	End	E-Value	Type
LIM	5	57	5.39e-11	SMART
NEBU	64	94	2.17e1	SMART
NEBU	168	197	1.94e-4	SMART
NEBU	202	232	1.39e-5	SMART
NEBU	239	268	2.23e-4	SMART
NEBU	308	337	2.83e-6	SMART
NEBU	346	376	3.82e-3	SMART
NEBU	382	412	1.18e-3	SMART
NEBU	450	480	8.97e-9	SMART
NEBU	485	515	1.73e-10	SMART
NEBU	521	551	8.12e-7	SMART
NEBU	555	585	1.73e-1	SMART
NEBU	590	620	2.33e-7	SMART
NEBU	621	651	1.49e-5	SMART
NEBU	655	686	5.12e-4	SMART
NEBU	689	719	8.12e-7	SMART
NEBU	724	754	2.64e-6	SMART
NEBU	760	790	3.48e-6	SMART
NEBU	798	828	2.35e-3	SMART
NEBU	833	863	6.11e-2	SMART
NEBU	864	894	1.69e-4	SMART
NEBU	899	929	3.88e-4	SMART
NEBU	932	962	4e-6	SMART
NEBU	967	997	4.22e-5	SMART
NEBU	1003	1033	2.64e-6	SMART
NEBU	1041	1071	3.68e-5	SMART
NEBU	1076	1106	4.16e-4	SMART
NEBU	1107	1137	1.1e-3	SMART
NEBU	1142	1172	1.68e1	SMART
NEBU	1175	1205	4.59e-6	SMART
NEBU	1210	1240	4.06e-7	SMART
NEBU	1246	1276	1.99e-1	SMART
NEBU	1284	1314	1.85e-1	SMART
NEBU	1319	1349	1.39e-5	SMART
NEBU	1350	1380	4.03e-2	SMART
NEBU	1385	1415	1.76e-2	SMART
NEBU	1418	1448	2.09e0	SMART
NEBU	1453	1483	6.4e-5	SMART
NEBU	1489	1519	8.63e-1	SMART
NEBU	1527	1557	1.33e-2	SMART
NEBU	1562	1592	1.84e-5	SMART
NEBU	1593	1623	7.24e-4	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000073536
AA Change: D1631N

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000073228
Gene: ENSMUSG00000049134
AA Change: D1631N

Domain	Start	End	E-Value	Type
LIM	5	57	5.39e-11	SMART
NEBU	64	94	2.17e1	SMART
NEBU	168	197	1.94e-4	SMART
NEBU	202	232	1.39e-5	SMART
NEBU	239	268	2.23e-4	SMART
NEBU	308	337	2.83e-6	SMART
NEBU	346	376	7.24e-4	SMART
NEBU	381	411	3.46e-1	SMART
NEBU	417	447	1.18e-3	SMART
NEBU	485	515	8.97e-9	SMART
NEBU	520	550	1.73e-10	SMART
NEBU	556	586	8.12e-7	SMART
NEBU	590	620	1.73e-1	SMART
NEBU	625	655	2.33e-7	SMART
NEBU	656	686	1.49e-5	SMART
NEBU	690	721	5.12e-4	SMART
NEBU	724	754	8.12e-7	SMART
NEBU	759	789	2.64e-6	SMART
NEBU	795	825	3.48e-6	SMART
NEBU	833	863	2.35e-3	SMART
NEBU	868	898	6.11e-2	SMART
NEBU	899	929	1.69e-4	SMART
NEBU	934	964	3.88e-4	SMART
NEBU	967	997	4e-6	SMART
NEBU	1002	1032	4.22e-5	SMART
NEBU	1038	1068	2.64e-6	SMART
NEBU	1076	1106	3.68e-5	SMART
NEBU	1111	1141	4.16e-4	SMART
NEBU	1142	1172	1.1e-3	SMART
NEBU	1177	1207	1.68e1	SMART
NEBU	1210	1240	4.59e-6	SMART
NEBU	1245	1275	4.06e-7	SMART
NEBU	1281	1311	1.99e-1	SMART
NEBU	1319	1349	1.85e-1	SMART
NEBU	1354	1384	1.39e-5	SMART
NEBU	1385	1415	4.03e-2	SMART
NEBU	1420	1450	1.76e-2	SMART
NEBU	1453	1483	2.09e0	SMART
NEBU	1488	1518	6.4e-5	SMART
NEBU	1524	1554	8.63e-1	SMART
NEBU	1562	1592	1.33e-2	SMART
NEBU	1597	1627	1.84e-5	SMART
NEBU	1628	1658	7.24e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000078284

SMART Domains

Protein: ENSMUSP00000077402
Gene: ENSMUSG00000025075

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
low complexity region	28	42	N/A	INTRINSIC
EGF	70	103	4.66e-6	SMART
EGF	108	142	3.97e0	SMART
EGF	147	182	2.26e-4	SMART
KR	186	272	2.72e-39	SMART
Tryp_SPc	307	544	1.47e-90	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000095947
AA Change: D1514N

PolyPhen 2 Score 0.180 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000093640
Gene: ENSMUSG00000049134
AA Change: D1514N

Domain	Start	End	E-Value	Type
NEBU	86	115	1.2e-6	SMART
NEBU	120	150	9.1e-8	SMART
NEBU	157	186	1.4e-6	SMART
NEBU	226	255	1.8e-8	SMART
NEBU	264	294	2.5e-5	SMART
NEBU	300	330	7.8e-6	SMART
NEBU	368	398	6e-11	SMART
NEBU	403	433	1.1e-12	SMART
NEBU	439	469	5.2e-9	SMART
NEBU	473	503	1.1e-3	SMART
NEBU	508	538	1.5e-9	SMART
NEBU	539	569	1e-7	SMART
NEBU	573	604	3.3e-6	SMART
NEBU	607	637	5.4e-9	SMART
NEBU	642	672	1.7e-8	SMART
NEBU	678	708	2.3e-8	SMART
NEBU	716	746	1.5e-5	SMART
NEBU	751	781	4.1e-4	SMART
NEBU	782	812	1.1e-6	SMART
NEBU	817	847	2.6e-6	SMART
NEBU	850	880	2.6e-8	SMART
NEBU	885	915	2.7e-7	SMART
NEBU	921	951	1.7e-8	SMART
NEBU	959	989	2.4e-7	SMART
NEBU	994	1024	2.7e-6	SMART
NEBU	1025	1055	7.2e-6	SMART
NEBU	1060	1090	1.1e-1	SMART
NEBU	1093	1123	3e-8	SMART
NEBU	1128	1158	2.6e-9	SMART
NEBU	1164	1194	1.3e-3	SMART
NEBU	1202	1232	1.2e-3	SMART
NEBU	1237	1267	8.8e-8	SMART
NEBU	1268	1298	2.7e-4	SMART
NEBU	1303	1333	1.2e-4	SMART
NEBU	1336	1366	1.4e-2	SMART
NEBU	1371	1401	4.3e-7	SMART
NEBU	1407	1437	5.6e-3	SMART
NEBU	1445	1475	8.8e-5	SMART
NEBU	1480	1510	1.2e-7	SMART
NEBU	1511	1541	4.8e-6	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000095948

SMART Domains

Protein: ENSMUSP00000093641
Gene: ENSMUSG00000025075

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
EGF	33	66	4.66e-6	SMART
EGF	71	105	3.97e0	SMART
EGF	110	145	2.26e-4	SMART
KR	149	235	2.72e-39	SMART
Tryp_SPc	270	507	1.47e-90	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000165522

SMART Domains

Protein: ENSMUSP00000130809
Gene: ENSMUSG00000025075

Domain	Start	End	E-Value	Type
Pfam:Trypsin	41	106	6.4e-16	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000166049

SMART Domains

Protein: ENSMUSP00000132444
Gene: ENSMUSG00000025075

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
low complexity region	28	42	N/A	INTRINSIC
EGF	70	103	4.66e-6	SMART
EGF	108	142	3.97e0	SMART
EGF	147	182	2.26e-4	SMART
KR	186	272	2.72e-39	SMART
Tryp_SPc	302	539	1.47e-90	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000166203
AA Change: D1595N

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000132582
Gene: ENSMUSG00000049134
AA Change: D1595N

Domain	Start	End	E-Value	Type
LIM	5	57	5.39e-11	SMART
NEBU	64	94	2.17e1	SMART
NEBU	168	197	1.94e-4	SMART
NEBU	202	232	1.39e-5	SMART
NEBU	239	268	2.23e-4	SMART
NEBU	308	337	2.83e-6	SMART
NEBU	346	376	7.24e-4	SMART
NEBU	381	411	3.46e-1	SMART
NEBU	417	447	1.18e-3	SMART
NEBU	485	515	8.97e-9	SMART
NEBU	520	550	1.06e-10	SMART
NEBU	554	584	1.73e-1	SMART
NEBU	589	619	2.33e-7	SMART
NEBU	620	650	1.49e-5	SMART
NEBU	654	685	5.12e-4	SMART
NEBU	688	718	8.12e-7	SMART
NEBU	723	753	2.64e-6	SMART
NEBU	759	789	3.48e-6	SMART
NEBU	797	827	2.35e-3	SMART
NEBU	832	862	6.11e-2	SMART
NEBU	863	893	1.69e-4	SMART
NEBU	898	928	3.88e-4	SMART
NEBU	931	961	4e-6	SMART
NEBU	966	996	4.22e-5	SMART
NEBU	1002	1032	2.64e-6	SMART
NEBU	1040	1070	3.68e-5	SMART
NEBU	1075	1105	4.16e-4	SMART
NEBU	1106	1136	1.1e-3	SMART
NEBU	1141	1171	1.68e1	SMART
NEBU	1174	1204	4.59e-6	SMART
NEBU	1209	1239	4.06e-7	SMART
NEBU	1245	1275	1.99e-1	SMART
NEBU	1283	1313	1.85e-1	SMART
NEBU	1318	1348	1.39e-5	SMART
NEBU	1349	1379	4.03e-2	SMART
NEBU	1384	1414	1.76e-2	SMART
NEBU	1417	1447	2.09e0	SMART
NEBU	1452	1482	6.4e-5	SMART
NEBU	1488	1518	8.63e-1	SMART
NEBU	1526	1556	1.33e-2	SMART
NEBU	1561	1591	1.84e-5	SMART
NEBU	1592	1622	7.24e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000167239

SMART Domains

Protein: ENSMUSP00000128196
Gene: ENSMUSG00000049134

Domain	Start	End	E-Value	Type
LIM	5	57	5.39e-11	SMART
NEBU	64	94	2.17e1	SMART
NEBU	168	197	1.94e-4	SMART
NEBU	202	232	1.39e-5	SMART
NEBU	239	268	2.23e-4	SMART
NEBU	308	337	2.83e-6	SMART
NEBU	346	376	3.82e-3	SMART
NEBU	382	412	1.18e-3	SMART
NEBU	450	480	8.97e-9	SMART
NEBU	485	515	1.73e-10	SMART
NEBU	521	551	8.12e-7	SMART
NEBU	555	585	1.73e-1	SMART
NEBU	590	620	2.33e-7	SMART
NEBU	621	651	1.49e-5	SMART
NEBU	655	686	5.12e-4	SMART
NEBU	689	719	8.12e-7	SMART
NEBU	724	754	2.64e-6	SMART
NEBU	760	790	3.48e-6	SMART
NEBU	798	828	2.35e-3	SMART
NEBU	833	863	6.11e-2	SMART
NEBU	864	894	1.69e-4	SMART
NEBU	899	929	3.88e-4	SMART
NEBU	932	962	4e-6	SMART
NEBU	967	997	4.22e-5	SMART
NEBU	1003	1033	2.64e-6	SMART
NEBU	1041	1071	3.68e-5	SMART
NEBU	1076	1106	4.16e-4	SMART
NEBU	1107	1137	1.1e-3	SMART
NEBU	1142	1172	1.68e1	SMART
NEBU	1175	1205	4.59e-6	SMART
NEBU	1210	1240	4.06e-7	SMART
NEBU	1246	1276	1.99e-1	SMART
NEBU	1284	1314	1.85e-1	SMART
NEBU	1319	1349	1.39e-5	SMART
NEBU	1350	1380	4.03e-2	SMART
NEBU	1385	1415	3.06e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000169099

SMART Domains

Protein: ENSMUSP00000125889
Gene: ENSMUSG00000049134

Domain	Start	End	E-Value	Type
NEBU	32	61	2.83e-6	SMART
NEBU	70	100	7.24e-4	SMART
NEBU	105	135	3.46e-1	SMART
NEBU	141	171	1.18e-3	SMART
NEBU	209	239	8.97e-9	SMART
NEBU	244	274	1.73e-10	SMART
NEBU	280	310	8.12e-7	SMART
NEBU	314	344	1.73e-1	SMART

Meta Mutation Damage Score

0.1337

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700011L22Rik	C	T	8: 79,248,293	E54K	probably benign	Het
Als2	A	C	1: 59,167,199	M1634R	probably damaging	Het
Ankfy1	T	C	11: 72,749,420	S531P	probably benign	Het
Atp6v1e1	A	G	6: 120,801,059	Y172H	probably benign	Het
Brd1	C	T	15: 88,717,040	V464M	probably damaging	Het
Btaf1	A	T	19: 36,986,548	T840S	probably benign	Het
Btaf1	A	T	19: 36,988,973	H1047L	probably benign	Het
Cacnb2	A	G	2: 14,824,263	D3G	possibly damaging	Het
Capn13	G	A	17: 73,339,401	P339L	probably benign	Het
Cgrrf1	G	T	14: 46,832,363	G30C	probably damaging	Het
Crnkl1	T	C	2: 145,919,795	D614G	probably benign	Het
Cyp2c23	C	T	19: 44,007,039	V430I	probably benign	Het
Dazl	G	A	17: 50,281,281	R289W	probably benign	Het
Dsg1b	C	T	18: 20,390,203	P96S	probably damaging	Het
Dusp14	A	G	11: 84,048,709	S169P	possibly damaging	Het
Egfem1	A	G	3: 29,151,926	D91G	probably benign	Het
Eif1	A	G	11: 100,320,824	K95E	probably damaging	Het
Fap	C	T	2: 62,546,650	V191I	probably benign	Het
Flnc	T	C	6: 29,447,404	Y1069H	probably damaging	Het
Fndc7	T	C	3: 108,869,148	T526A	possibly damaging	Het
Fras1	A	T	5: 96,733,932	T2508S	probably benign	Het
Gast	T	C	11: 100,336,810	S73P	probably damaging	Het
Grap2	A	G	15: 80,623,746	T4A	possibly damaging	Het
Grm8	T	G	6: 28,125,636	N164H	possibly damaging	Het
Hyal5	A	T	6: 24,876,524	H132L	probably benign	Het
Iqcm	C	A	8: 75,669,393	T188K	possibly damaging	Het
Kank4	A	G	4: 98,780,133	S26P	probably damaging	Het
Lipo1	C	T	19: 33,784,857	C80Y	probably damaging	Het
Lrrk2	A	G	15: 91,737,111	R963G	probably benign	Het
Lrtm2	G	A	6: 119,317,483	T229I	probably damaging	Het
Mb21d2	A	T	16: 28,828,003	D406E	possibly damaging	Het
Meikin	T	A	11: 54,399,871		probably null	Het
Mybl1	A	T	1: 9,673,214	F538I	probably damaging	Het
Nectin2	T	C	7: 19,717,636	D491G	probably benign	Het
Nf1	A	G	11: 79,559,521	D511G	probably null	Het
Nid2	T	C	14: 19,809,997	C1328R	probably damaging	Het
Nlrp1a	T	A	11: 71,122,703	M574L	probably benign	Het
Nrg3	G	A	14: 38,376,434	P496S	probably damaging	Het
Olfr1287	A	T	2: 111,449,565	I142F	probably benign	Het
Pak3	G	A	X: 143,709,731	V87I	probably damaging	Het
Phf8	T	C	X: 151,572,576	S512P	possibly damaging	Het
Phkb	G	T	8: 85,922,229	E225*	probably null	Het
Plaa	T	C	4: 94,569,888	D615G	probably damaging	Het
Prdm12	A	G	2: 31,651,947	K223E	probably damaging	Het
Prkd1	C	A	12: 50,383,422	R634L	possibly damaging	Het
Rassf3	T	A	10: 121,414,366	I181F	possibly damaging	Het
Samd8	G	A	14: 21,775,065	V30M	probably damaging	Het
Sema4f	A	T	6: 82,918,627	H308Q	possibly damaging	Het
Skint4	G	T	4: 112,118,181	V113L	probably damaging	Het
Slc16a10	G	C	10: 40,056,624	H314D	possibly damaging	Het
Slpi	A	G	2: 164,356,238	L12P	probably damaging	Het
Smco1	A	G	16: 32,273,898	Y129C	probably benign	Het
Spag17	A	G	3: 100,053,853	K985R	possibly damaging	Het
Stc1	T	C	14: 69,038,475	I239T	probably benign	Het
Suclg2	A	T	6: 95,497,668	I372N	probably damaging	Het
Tmed4	C	A	11: 6,274,233	V80F	probably damaging	Het
Tnpo2	T	A	8: 85,055,171		probably null	Het
Top1	A	G	2: 160,702,768	H268R	probably damaging	Het
Triobp	A	T	15: 78,973,700	Q1167L	probably benign	Het
Txndc16	G	A	14: 45,151,352	P536L	possibly damaging	Het
Usp13	C	T	3: 32,881,508	A360V	possibly damaging	Het
Vhl	G	A	6: 113,629,462	V147I	probably benign	Het
Vldlr	A	T	19: 27,217,621	T3S	probably damaging	Het
Vps54	T	A	11: 21,268,832	D130E	probably benign	Het
Zfp808	T	A	13: 62,172,083	H375Q	probably damaging	Het
Zfp87	A	T	13: 67,521,215	N37K	probably damaging	Het

Other mutations in Nrap

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00500:Nrap	APN	19	56372909	missense	probably damaging	1.00
IGL00570:Nrap	APN	19	56338113	missense	probably benign	0.10
IGL00946:Nrap	APN	19	56340626	splice site	probably null
IGL01070:Nrap	APN	19	56329084	missense	probably damaging	1.00
IGL01111:Nrap	APN	19	56345558	missense	probably damaging	1.00
IGL01138:Nrap	APN	19	56355538	missense	probably damaging	1.00
IGL01290:Nrap	APN	19	56361748	missense	probably damaging	1.00
IGL01352:Nrap	APN	19	56379836	missense	probably benign	0.00
IGL01372:Nrap	APN	19	56329102	critical splice acceptor site	probably null
IGL01395:Nrap	APN	19	56361793	missense	probably damaging	1.00
IGL01413:Nrap	APN	19	56389391	missense	probably damaging	0.99
IGL01734:Nrap	APN	19	56350309	missense	probably damaging	1.00
IGL01933:Nrap	APN	19	56388818	missense	probably damaging	1.00
IGL02156:Nrap	APN	19	56321000	missense	probably damaging	1.00
IGL02415:Nrap	APN	19	56382309	missense	probably damaging	1.00
IGL02447:Nrap	APN	19	56345519	nonsense	probably null
IGL02864:Nrap	APN	19	56350374	missense	probably damaging	1.00
IGL02993:Nrap	APN	19	56345533	missense	probably damaging	1.00
IGL03003:Nrap	APN	19	56321952	missense	probably damaging	1.00
IGL03006:Nrap	APN	19	56347164	missense	probably benign	0.02
IGL03084:Nrap	APN	19	56365454	missense	probably damaging	1.00
IGL03136:Nrap	APN	19	56342255	missense	possibly damaging	0.69
IGL03272:Nrap	APN	19	56345568	intron	probably benign
IGL03389:Nrap	APN	19	56351716	missense	probably benign	0.10
R0116:Nrap	UTSW	19	56355546	missense	probably damaging	1.00
R0374:Nrap	UTSW	19	56351622	missense	probably damaging	1.00
R0715:Nrap	UTSW	19	56357325	missense	probably damaging	0.98
R0828:Nrap	UTSW	19	56345558	missense	probably damaging	1.00
R0883:Nrap	UTSW	19	56345474	missense	probably damaging	1.00
R1416:Nrap	UTSW	19	56327293	missense	possibly damaging	0.60
R1459:Nrap	UTSW	19	56384130	missense	probably benign	0.00
R1616:Nrap	UTSW	19	56389823	missense	probably damaging	1.00
R1676:Nrap	UTSW	19	56335255	missense	probably damaging	1.00
R1687:Nrap	UTSW	19	56355529	missense	probably damaging	0.99
R1766:Nrap	UTSW	19	56335042	missense	probably damaging	0.99
R1792:Nrap	UTSW	19	56379158	missense	probably benign	0.00
R1817:Nrap	UTSW	19	56384055	unclassified	probably benign
R1972:Nrap	UTSW	19	56357353	missense	probably damaging	1.00
R1982:Nrap	UTSW	19	56384105	missense	probably damaging	0.99
R2258:Nrap	UTSW	19	56321962	missense	possibly damaging	0.80
R2448:Nrap	UTSW	19	56322030	missense	possibly damaging	0.90
R3034:Nrap	UTSW	19	56364005	missense	probably damaging	1.00
R3804:Nrap	UTSW	19	56321779	missense	probably damaging	1.00
R3923:Nrap	UTSW	19	56380256	missense	probably damaging	0.99
R3964:Nrap	UTSW	19	56342144	missense	probably damaging	1.00
R3965:Nrap	UTSW	19	56342144	missense	probably damaging	1.00
R3966:Nrap	UTSW	19	56342144	missense	probably damaging	1.00
R3980:Nrap	UTSW	19	56381552	missense	probably benign	0.01
R4182:Nrap	UTSW	19	56350327	missense	probably damaging	1.00
R4499:Nrap	UTSW	19	56351481	missense	probably damaging	0.97
R4573:Nrap	UTSW	19	56342338	critical splice acceptor site	probably null
R4603:Nrap	UTSW	19	56335024	critical splice donor site	probably null
R4689:Nrap	UTSW	19	56386026	missense	probably damaging	0.97
R4749:Nrap	UTSW	19	56380237	missense	probably damaging	0.96
R4845:Nrap	UTSW	19	56351470	missense	probably benign	0.16
R4937:Nrap	UTSW	19	56347220	missense	probably damaging	1.00
R4962:Nrap	UTSW	19	56378143	missense	probably damaging	1.00
R5156:Nrap	UTSW	19	56371845	missense	possibly damaging	0.94
R5181:Nrap	UTSW	19	56345528	missense	possibly damaging	0.85
R5202:Nrap	UTSW	19	56335151	missense	probably damaging	1.00
R5262:Nrap	UTSW	19	56320223	missense	possibly damaging	0.95
R5301:Nrap	UTSW	19	56379109	missense	probably damaging	1.00
R5380:Nrap	UTSW	19	56381603	missense	probably damaging	1.00
R5576:Nrap	UTSW	19	56321982	missense	probably damaging	0.99
R5631:Nrap	UTSW	19	56354121	missense	probably benign	0.19
R5754:Nrap	UTSW	19	56389484	missense	possibly damaging	0.55
R5799:Nrap	UTSW	19	56342169	nonsense	probably null
R5899:Nrap	UTSW	19	56340574	missense	possibly damaging	0.80
R5910:Nrap	UTSW	19	56342311	missense	probably benign	0.00
R5994:Nrap	UTSW	19	56351599	nonsense	probably null
R6124:Nrap	UTSW	19	56386026	missense	probably damaging	0.97
R6149:Nrap	UTSW	19	56389453	missense	possibly damaging	0.79
R6182:Nrap	UTSW	19	56361698	missense	probably benign
R6245:Nrap	UTSW	19	56354221	missense	probably damaging	1.00
R6245:Nrap	UTSW	19	56379875	missense	possibly damaging	0.80
R6270:Nrap	UTSW	19	56320198	missense	probably benign	0.00
R6274:Nrap	UTSW	19	56361721	missense	probably benign	0.21
R6340:Nrap	UTSW	19	56347184	missense	probably damaging	1.00
R6547:Nrap	UTSW	19	56351566	missense	probably benign	0.00
R6734:Nrap	UTSW	19	56345509	missense	probably damaging	0.99
R6770:Nrap	UTSW	19	56382537	splice site	probably null
R6812:Nrap	UTSW	19	56351676	missense	probably damaging	1.00
R6843:Nrap	UTSW	19	56380219	missense	probably damaging	1.00
R7207:Nrap	UTSW	19	56345521	missense	probably damaging	1.00
R7214:Nrap	UTSW	19	56378135	missense	probably benign	0.09
R7313:Nrap	UTSW	19	56342268	missense	probably damaging	0.97
R7515:Nrap	UTSW	19	56366427	missense	possibly damaging	0.94
R7662:Nrap	UTSW	19	56320283	missense	probably benign	0.00
R7819:Nrap	UTSW	19	56335288	missense	probably benign
R7836:Nrap	UTSW	19	56350297	missense	probably benign	0.00
R7895:Nrap	UTSW	19	56354152	missense	probably benign	0.00
R8041:Nrap	UTSW	19	56364336	nonsense	probably null
R8046:Nrap	UTSW	19	56320251	missense	possibly damaging	0.46
R8066:Nrap	UTSW	19	56354130	missense	possibly damaging	0.94
R8129:Nrap	UTSW	19	56366636	splice site	probably null
R8188:Nrap	UTSW	19	56336578	nonsense	probably null
R8323:Nrap	UTSW	19	56389823	missense	probably benign	0.00
R8353:Nrap	UTSW	19	56323920	missense	probably damaging	1.00
R8453:Nrap	UTSW	19	56323920	missense	probably damaging	1.00
R8693:Nrap	UTSW	19	56321952	missense	probably damaging	1.00
R8703:Nrap	UTSW	19	56335271	missense	probably damaging	1.00
R8810:Nrap	UTSW	19	56364411	critical splice acceptor site	probably benign
R8872:Nrap	UTSW	19	56320195	makesense	probably null
R8980:Nrap	UTSW	19	56355538	missense	probably damaging	1.00
R9201:Nrap	UTSW	19	56351661	missense	probably damaging	1.00
R9229:Nrap	UTSW	19	56321907	missense	probably benign	0.01
R9235:Nrap	UTSW	19	56342328	nonsense	probably null
R9323:Nrap	UTSW	19	56389823	missense	probably benign	0.00
R9327:Nrap	UTSW	19	56351668	missense	probably benign	0.25
R9329:Nrap	UTSW	19	56361845	missense	probably damaging	1.00
X0028:Nrap	UTSW	19	56335220	nonsense	probably null
Z1176:Nrap	UTSW	19	56345517	frame shift	probably null
Z1177:Nrap	UTSW	19	56338092	missense	probably damaging	1.00
Z1177:Nrap	UTSW	19	56344764	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CCCAATCCTGAGAAAGCATTTGG -3'
(R):5'- AGAGGCATTTCTGAGGGACC -3'

Sequencing Primer
(F):5'- CCTGAGAAAGCATTTGGTACATTTG -3'
(R):5'- GTACCGTAGCATCAGTGACGAC -3'

Posted On

2015-03-25