Mutagenetix > Incidental Mutation

Incidental Mutation 'R3768:Tarm1'

273113

Institutional Source

Beutler Lab

Gene Symbol

Tarm1

Ensembl Gene

ENSMUSG00000053338

Gene Name

T cell-interacting, activating receptor on myeloid cells 1

Synonyms

Gm9904, 9930022N03Rik

MMRRC Submission

040745-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.071) question?

Stock #

R3768 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

3535016-3551140 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 3546097 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 69 (S69T)

Ref Sequence

ENSEMBL: ENSMUSP00000145188 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000065703] [ENSMUST00000203020] [ENSMUST00000203821]

AlphaFold

B6A8R8

Predicted Effect

probably benign
Transcript: ENSMUST00000065703
AA Change: S57T

PolyPhen 2 Score 0.075 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000069745
Gene: ENSMUSG00000053338
AA Change: S57T

Domain	Start	End	E-Value	Type
signal peptide	1	16	N/A	INTRINSIC
IG_like	34	120	9.45e0	SMART
IG	131	217	5.28e-3	SMART
transmembrane domain	256	278	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000203020
AA Change: S69T

PolyPhen 2 Score 0.105 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000145188
Gene: ENSMUSG00000053338
AA Change: S69T

Domain	Start	End	E-Value	Type
signal peptide	1	16	N/A	INTRINSIC
IG_like	40	104	4.6e-3	SMART
IG_like	92	198	4.1e-2	SMART
IG	131	217	2.2e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000203821
AA Change: S69T

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000145491
Gene: ENSMUSG00000053338
AA Change: S69T

Domain	Start	End	E-Value	Type
signal peptide	1	16	N/A	INTRINSIC
IG_like	34	120	9.45e0	SMART
IG	131	217	5.28e-3	SMART
transmembrane domain	256	278	N/A	INTRINSIC

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca5	T	C	11: 110,204,217 (GRCm39)	R353G	probably benign	Het
Ank1	A	G	8: 23,606,202 (GRCm39)	D1153G	possibly damaging	Het
Ankrd12	T	C	17: 66,292,715 (GRCm39)	Y906C	probably benign	Het
Apol9a	G	C	15: 77,288,596 (GRCm39)	T257S	probably benign	Het
Arhgap23	A	T	11: 97,366,932 (GRCm39)	D1071V	probably damaging	Het
Arid4a	A	G	12: 71,113,893 (GRCm39)	K160R	probably damaging	Het
Atp8a2	T	A	14: 60,281,785 (GRCm39)	H142L	probably benign	Het
B3gnt2	T	C	11: 22,786,765 (GRCm39)	K141R	probably damaging	Het
Btbd7	G	A	12: 102,761,451 (GRCm39)	P578L	probably damaging	Het
C3	C	T	17: 57,512,303 (GRCm39)	D1542N	possibly damaging	Het
Cmya5	A	T	13: 93,233,201 (GRCm39)	I629K	possibly damaging	Het
Cnga4	A	G	7: 105,056,887 (GRCm39)	N330S	probably damaging	Het
Cplx4	T	C	18: 66,102,998 (GRCm39)	T41A	probably benign	Het
Cxcl15	A	C	5: 90,949,303 (GRCm39)	D156A	unknown	Het
D630045J12Rik	A	G	6: 38,119,844 (GRCm39)	S1633P	probably damaging	Het
Dock10	T	A	1: 80,510,085 (GRCm39)	N1581I	probably damaging	Het
Dock7	T	C	4: 98,859,066 (GRCm39)	T1409A	probably benign	Het
Dscaml1	T	A	9: 45,643,435 (GRCm39)	F1285I	possibly damaging	Het
Fgf10	G	T	13: 118,918,083 (GRCm39)	V124F	probably damaging	Het
Ifna7	T	C	4: 88,734,964 (GRCm39)	V167A	probably damaging	Het
Kctd19	T	C	8: 106,123,112 (GRCm39)	T101A	probably benign	Het
Klf3	A	G	5: 64,984,560 (GRCm39)		probably null	Het
Krtap12-1	G	T	10: 77,556,729 (GRCm39)	V91L	probably benign	Het
Lrp2	C	T	2: 69,335,449 (GRCm39)	D1425N	probably benign	Het
Mgl2	T	C	11: 70,026,659 (GRCm39)	L128P	probably damaging	Het
Mypn	C	A	10: 62,961,486 (GRCm39)	L1035F	possibly damaging	Het
Ncor2	A	G	5: 125,105,751 (GRCm39)	V1613A	probably damaging	Het
Nlgn2	C	T	11: 69,719,230 (GRCm39)	V207I	possibly damaging	Het
Or10v1	G	A	19: 11,873,676 (GRCm39)	G97D	probably damaging	Het
Or1ad6	A	G	11: 50,860,385 (GRCm39)	D180G	probably damaging	Het
Or2y14	T	C	11: 49,404,600 (GRCm39)	I45T	probably damaging	Het
Or4d10c	A	G	19: 12,065,304 (GRCm39)	I284T	probably damaging	Het
Osbpl3	G	A	6: 50,324,982 (GRCm39)	P172L	possibly damaging	Het
Pabpc4	T	A	4: 123,188,405 (GRCm39)	V338D	probably damaging	Het
Pdia6	T	C	12: 17,320,457 (GRCm39)	V32A	probably damaging	Het
Pik3c3	T	A	18: 30,466,326 (GRCm39)	S792R	probably damaging	Het
Pla2g5	C	G	4: 138,528,746 (GRCm39)	C70S	probably damaging	Het
Pole4	G	A	6: 82,599,095 (GRCm39)	R119C	possibly damaging	Het
Ppm1h	G	T	10: 122,740,027 (GRCm39)	L367F	probably damaging	Het
Ptpru	C	T	4: 131,535,735 (GRCm39)	C414Y	probably damaging	Het
Rhot2	T	C	17: 26,059,521 (GRCm39)	D407G	probably benign	Het
Rufy4	T	C	1: 74,186,822 (GRCm39)	C537R	probably damaging	Het
Sap25	T	A	5: 137,640,632 (GRCm39)	F171I	probably damaging	Het
Serpinb11	T	C	1: 107,305,392 (GRCm39)		probably null	Het
Ugcg	C	T	4: 59,207,798 (GRCm39)	P46S	probably benign	Het
Usp36	T	C	11: 118,153,878 (GRCm39)	K846R	probably damaging	Het
Zfp229	A	T	17: 21,964,844 (GRCm39)	H358L	probably damaging	Het
Zfp747l1	A	G	7: 126,984,035 (GRCm39)		probably benign	Het

Other mutations in Tarm1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03229:Tarm1	APN	7	3,545,413 (GRCm39)	missense	probably damaging	1.00
R0096:Tarm1	UTSW	7	3,546,067 (GRCm39)	missense	probably benign	0.23
R0282:Tarm1	UTSW	7	3,546,006 (GRCm39)	missense	probably damaging	1.00
R0308:Tarm1	UTSW	7	3,545,187 (GRCm39)	splice site	probably benign
R4732:Tarm1	UTSW	7	3,545,416 (GRCm39)	nonsense	probably null
R4733:Tarm1	UTSW	7	3,545,416 (GRCm39)	nonsense	probably null
R4982:Tarm1	UTSW	7	3,537,612 (GRCm39)	missense	probably damaging	1.00
R5336:Tarm1	UTSW	7	3,546,084 (GRCm39)	missense	probably damaging	0.99
R6128:Tarm1	UTSW	7	3,537,720 (GRCm39)	missense	probably benign	0.04
R6746:Tarm1	UTSW	7	3,550,978 (GRCm39)	missense	probably benign	0.10
R6892:Tarm1	UTSW	7	3,546,006 (GRCm39)	missense	probably damaging	1.00
R7003:Tarm1	UTSW	7	3,545,939 (GRCm39)	critical splice donor site	probably null
R7414:Tarm1	UTSW	7	3,545,318 (GRCm39)	missense	probably benign	0.05
R8439:Tarm1	UTSW	7	3,546,037 (GRCm39)	missense	possibly damaging	0.69
R8925:Tarm1	UTSW	7	3,537,719 (GRCm39)	missense	possibly damaging	0.94
R8927:Tarm1	UTSW	7	3,537,719 (GRCm39)	missense	possibly damaging	0.94
R9608:Tarm1	UTSW	7	3,551,062 (GRCm39)	start gained	probably benign
R9687:Tarm1	UTSW	7	3,544,457 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- AGACCATCCTCACCTGTGAC -3'
(R):5'- ACACTGGTGTTATTTCTCAGGTGTC -3'

Sequencing Primer
(F):5'- CCAACAAGAAAAGGGCATTGCTG -3'
(R):5'- CAACTCAGGTGTTTGCGACCATG -3'

Posted On

2015-03-25