Mutagenetix > Incidental Mutation

Incidental Mutation 'R3498:Vmn1r176'

273657

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r176

Ensembl Gene

ENSMUSG00000096859

Gene Name

vomeronasal 1 receptor 176

Synonyms

Gm5998

MMRRC Submission

040661-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.062) question?

Stock #

R3498 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

23833840-23837372 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 23835242 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Isoleucine at position 162 (K162I)

Ref Sequence

ENSEMBL: ENSMUSP00000153879 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171073] [ENSMUST00000226319] [ENSMUST00000226767] [ENSMUST00000227129] [ENSMUST00000227661] [ENSMUST00000228280] [ENSMUST00000228793]

AlphaFold

G3UW31

Predicted Effect

probably benign
Transcript: ENSMUST00000171073
AA Change: K162I

PolyPhen 2 Score 0.121 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000132168
Gene: ENSMUSG00000096859
AA Change: K162I

Domain	Start	End	E-Value	Type
Pfam:7tm_1	5	288	5.4e-6	PFAM
Pfam:TAS2R	8	295	1.8e-13	PFAM
Pfam:V1R	41	295	1.7e-21	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000226319
AA Change: K162I

PolyPhen 2 Score 0.121 (Sensitivity: 0.93; Specificity: 0.86)

Predicted Effect

probably benign
Transcript: ENSMUST00000226767
AA Change: K162I

PolyPhen 2 Score 0.121 (Sensitivity: 0.93; Specificity: 0.86)

Predicted Effect

probably benign
Transcript: ENSMUST00000227129
AA Change: K162I

PolyPhen 2 Score 0.121 (Sensitivity: 0.93; Specificity: 0.86)

Predicted Effect

probably benign
Transcript: ENSMUST00000227661
AA Change: K162I

PolyPhen 2 Score 0.121 (Sensitivity: 0.93; Specificity: 0.86)

Predicted Effect

probably benign
Transcript: ENSMUST00000228280
AA Change: K162I

PolyPhen 2 Score 0.121 (Sensitivity: 0.93; Specificity: 0.86)

Predicted Effect

probably benign
Transcript: ENSMUST00000228793
AA Change: K162I

PolyPhen 2 Score 0.121 (Sensitivity: 0.93; Specificity: 0.86)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.6%
20x: 96.0%

Validation Efficiency

100% (43/43)

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700001C19Rik	AC	A	17: 47,433,423		probably benign	Het
A3galt2	T	C	4: 128,755,557	F6L	probably benign	Het
Aurkc	A	G	7: 7,000,030	I175V	probably damaging	Het
Azi2	A	G	9: 118,049,407	D105G	probably damaging	Het
Bcat1	A	G	6: 145,019,342	V45A	probably damaging	Het
Cass4	C	T	2: 172,432,558	P753L	probably damaging	Het
Ddx42	A	G	11: 106,231,193	E178G	possibly damaging	Het
Dmpk	T	C	7: 19,086,381	I101T	probably damaging	Het
Dnah17	C	T	11: 118,080,849		probably benign	Het
Fosb	C	T	7: 19,306,632	R161H	probably damaging	Het
Gm6729	T	C	10: 86,540,718		noncoding transcript	Het
Gnb1	T	A	4: 155,555,026	N237K	possibly damaging	Het
Gpr35	A	G	1: 92,983,391	Y275C	probably damaging	Het
Hmcn1	A	T	1: 150,605,102	I4441N	probably damaging	Het
Ighe	G	A	12: 113,271,374	Q389*	probably null	Het
Kcnj11	T	C	7: 46,099,602	D23G	probably damaging	Het
Lats2	G	T	14: 57,722,466	A191E	possibly damaging	Het
Lyplal1	A	G	1: 186,088,660	S197P	possibly damaging	Het
Map4	G	T	9: 110,035,212	V502L	probably benign	Het
Mgat5	A	G	1: 127,384,834	M237V	possibly damaging	Het
Mindy4	A	G	6: 55,216,525	R68G	probably benign	Het
Nell1	T	A	7: 50,258,179	V362E	possibly damaging	Het
Olfr1031	T	A	2: 85,992,430	F204L	probably benign	Het
Olfr792	T	A	10: 129,540,909	I124N	probably damaging	Het
P4ha2	T	C	11: 54,119,253	Y279H	probably benign	Het
Pcid2	A	G	8: 13,100,413	V13A	possibly damaging	Het
Polr2j	T	C	5: 136,122,770	I116T	probably benign	Het
Prdm9	A	G	17: 15,562,945		probably benign	Het
Prr5	T	A	15: 84,703,144	V365E	probably benign	Het
Ptprf	A	T	4: 118,224,930	I1037N	probably damaging	Het
Ranbp17	GCCTGGATACTGACC	GCC	11: 33,219,203		probably benign	Het
Sde2	T	A	1: 180,858,185	C101S	probably damaging	Het
Sec1	T	C	7: 45,679,239	H128R	probably damaging	Het
Serpinb6a	A	T	13: 33,918,781	M253K	probably damaging	Het
Slc1a4	A	T	11: 20,313,973	I248N	probably damaging	Het
Slc22a4	T	G	11: 53,992,053	K328N	probably benign	Het
Slc24a4	A	T	12: 102,234,692	K278N	probably benign	Het
Slc6a21	T	A	7: 45,280,842	W222R	probably damaging	Het
Slc7a2	A	G	8: 40,912,530	E466G	probably benign	Het
Sspo	A	G	6: 48,467,980	T2133A	possibly damaging	Het
Taco1	A	G	11: 106,072,538	M172V	probably benign	Het
Tmem127	T	A	2: 127,256,120	H36Q	probably benign	Het
Tmem229b-ps	C	T	10: 53,475,127		noncoding transcript	Het
Vac14	A	G	8: 110,671,090	D479G	probably benign	Het

Other mutations in Vmn1r176

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01100:Vmn1r176	APN	7	23835624	missense	probably benign	0.04
IGL03004:Vmn1r176	APN	7	23835277	missense	probably damaging	1.00
PIT4151001:Vmn1r176	UTSW	7	23835383	missense	probably damaging	1.00
R0195:Vmn1r176	UTSW	7	23835585	missense	probably benign	0.10
R1186:Vmn1r176	UTSW	7	23835626	missense	probably damaging	1.00
R1531:Vmn1r176	UTSW	7	23835111	missense	possibly damaging	0.95
R1680:Vmn1r176	UTSW	7	23835381	missense	probably damaging	0.99
R1770:Vmn1r176	UTSW	7	23835521	missense	probably benign	0.06
R1803:Vmn1r176	UTSW	7	23835184	missense	probably damaging	1.00
R1970:Vmn1r176	UTSW	7	23834948	missense	probably benign	0.01
R2092:Vmn1r176	UTSW	7	23835153	missense	probably damaging	1.00
R4832:Vmn1r176	UTSW	7	23835038	missense	possibly damaging	0.67
R5712:Vmn1r176	UTSW	7	23835500	missense	probably benign	0.00
R6965:Vmn1r176	UTSW	7	23835674	missense	possibly damaging	0.78
R7105:Vmn1r176	UTSW	7	23835323	nonsense	probably null
R7839:Vmn1r176	UTSW	7	23834969	missense	possibly damaging	0.91
R8262:Vmn1r176	UTSW	7	23835453	missense	probably benign	0.29
R8870:Vmn1r176	UTSW	7	23835055	missense	probably damaging	1.00
R9147:Vmn1r176	UTSW	7	23835360	missense	probably benign	0.12
R9432:Vmn1r176	UTSW	7	23835318	missense	probably damaging	1.00
Z1088:Vmn1r176	UTSW	7	23835173	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- GTCTCAGCATGAACTCTGAGC -3'
(R):5'- GTACAAACATGTGCTCCACC -3'

Sequencing Primer
(F):5'- AGCATGAACTCTGAGCTTCTG -3'
(R):5'- AAACATGTGCTCCACCTGCTC -3'

Posted On

2015-04-02