Mutagenetix > Incidental Mutation

Incidental Mutation 'R3499:Gpr35'

273684

Institutional Source

Beutler Lab

Gene Symbol

Gpr35

Ensembl Gene

ENSMUSG00000026271

Gene Name

G protein-coupled receptor 35

Synonyms

MMRRC Submission

040662-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3499 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

92878587-92914113 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 92911113 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 275 (Y275C)

Ref Sequence

ENSEMBL: ENSMUSP00000139648 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000027489] [ENSMUST00000064480] [ENSMUST00000169198] [ENSMUST00000185421] [ENSMUST00000186298] [ENSMUST00000189697]

AlphaFold

Q9ES90

Predicted Effect

probably damaging
Transcript: ENSMUST00000027489
AA Change: Y59C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000027489
Gene: ENSMUSG00000026271
AA Change: Y59C

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	28	289	3.5e-7	PFAM
Pfam:7tm_1	34	275	1.8e-34	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000064480
AA Change: Y275C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000070832
Gene: ENSMUSG00000026271
AA Change: Y275C

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	28	289	3.5e-7	PFAM
Pfam:7tm_1	34	275	3.6e-42	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000169198
AA Change: Y275C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000126914
Gene: ENSMUSG00000026271
AA Change: Y275C

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	28	289	3.5e-7	PFAM
Pfam:7tm_1	34	275	3.6e-42	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000185421

Predicted Effect

probably damaging
Transcript: ENSMUST00000186298
AA Change: Y275C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000139648
Gene: ENSMUSG00000026271
AA Change: Y275C

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	28	289	3.5e-7	PFAM
Pfam:7tm_1	34	275	3.6e-42	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000189697

SMART Domains

Protein: ENSMUSP00000139787
Gene: ENSMUSG00000026271

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	28	147	5.7e-6	PFAM
Pfam:7tm_1	34	147	1.4e-24	PFAM

Meta Mutation Damage Score

0.8052

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.7%
20x: 96.3%

Validation Efficiency

100% (46/46)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
6430571L13Rik	A	T	9: 107,219,678 (GRCm39)	T44S	probably damaging	Het
Cass4	C	T	2: 172,274,478 (GRCm39)	P753L	probably damaging	Het
Cimip3	AC	A	17: 47,744,348 (GRCm39)		probably benign	Het
Ctif	A	T	18: 75,744,828 (GRCm39)	L92Q	possibly damaging	Het
Dnah11	C	A	12: 117,874,758 (GRCm39)	D4036Y	probably damaging	Het
Dnah17	C	T	11: 117,971,675 (GRCm39)		probably benign	Het
Dnah6	G	C	6: 73,009,616 (GRCm39)	A3725G	probably benign	Het
Ebag9	A	G	15: 44,493,528 (GRCm39)	N117D	probably benign	Het
Elf4	C	T	X: 47,507,942 (GRCm39)	E158K	probably benign	Het
Ephb6	C	T	6: 41,593,093 (GRCm39)	R444*	probably null	Het
Fcgbpl1	T	A	7: 27,853,980 (GRCm39)	I1648K	probably benign	Het
Fndc1	C	T	17: 7,972,416 (GRCm39)	M1505I	possibly damaging	Het
Hmcn1	A	T	1: 150,480,853 (GRCm39)	I4441N	probably damaging	Het
Kif13a	T	C	13: 46,978,815 (GRCm39)	D195G	probably damaging	Het
Kirrel1	C	T	3: 86,996,458 (GRCm39)	M380I	probably null	Het
Ksr2	C	A	5: 117,827,640 (GRCm39)	T473K	probably damaging	Het
Kxd1	T	C	8: 70,966,632 (GRCm39)		probably null	Het
Lyplal1	A	G	1: 185,820,857 (GRCm39)	S197P	possibly damaging	Het
Macf1	A	T	4: 123,421,098 (GRCm39)	L52*	probably null	Het
Mrgprb5	A	G	7: 47,818,661 (GRCm39)	S25P	probably benign	Het
Nucb1	T	C	7: 45,148,300 (GRCm39)	H171R	probably benign	Het
Or10d1c	A	G	9: 38,893,761 (GRCm39)	L193P	probably damaging	Het
Or4a15	A	T	2: 89,193,294 (GRCm39)	F160I	probably benign	Het
Or56b35	G	A	7: 104,963,607 (GRCm39)	C132Y	probably damaging	Het
Or8h8	A	G	2: 86,753,373 (GRCm39)	F168L	possibly damaging	Het
Or8k18	A	G	2: 86,085,185 (GRCm39)	M284T	possibly damaging	Het
Prkdc	A	T	16: 15,585,889 (GRCm39)	I2476F	probably damaging	Het
Ptprf	A	T	4: 118,082,127 (GRCm39)	I1037N	probably damaging	Het
Ralgapa1	T	G	12: 55,741,928 (GRCm39)		probably benign	Het
Ranbp17	GCCTGGATACTGACC	GCC	11: 33,169,203 (GRCm39)		probably benign	Het
Sla2	G	A	2: 156,717,862 (GRCm39)	R137C	probably damaging	Het
Slc28a3	C	T	13: 58,721,253 (GRCm39)		probably benign	Het
Slc44a4	A	G	17: 35,140,656 (GRCm39)	D283G	probably benign	Het
Slc6a9	A	G	4: 117,714,000 (GRCm39)	I105V	probably benign	Het
Smgc	A	G	15: 91,726,206 (GRCm39)	T51A	possibly damaging	Het
Smyd5	T	A	6: 85,415,172 (GRCm39)	L60H	probably damaging	Het
Spred1	A	T	2: 117,005,867 (GRCm39)	M210L	probably benign	Het
Syne2	G	A	12: 76,101,752 (GRCm39)		probably null	Het
Tab3	T	C	X: 84,658,727 (GRCm39)	I418T	probably benign	Het
Tbc1d12	A	T	19: 38,884,478 (GRCm39)	E343V	possibly damaging	Het
Tktl2	T	A	8: 66,965,897 (GRCm39)	I485N	probably damaging	Het
Tmem181a	T	A	17: 6,330,894 (GRCm39)	Y51*	probably null	Het
Tmem229b-ps	C	T	10: 53,351,223 (GRCm39)		noncoding transcript	Het
Trip11	A	G	12: 101,859,953 (GRCm39)	I307T	possibly damaging	Het
Ttf1	T	C	2: 28,955,499 (GRCm39)	S288P	possibly damaging	Het
Zfp395	G	T	14: 65,628,742 (GRCm39)	R198L	possibly damaging	Het

Other mutations in Gpr35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00509:Gpr35	APN	1	92,910,594 (GRCm39)	missense	probably damaging	0.99
IGL03102:Gpr35	APN	1	92,910,299 (GRCm39)	missense	probably benign	0.00
PIT4280001:Gpr35	UTSW	1	92,910,356 (GRCm39)	missense	probably damaging	1.00
R0015:Gpr35	UTSW	1	92,910,954 (GRCm39)	missense	probably damaging	1.00
R0412:Gpr35	UTSW	1	92,910,506 (GRCm39)	missense	probably benign	0.01
R1203:Gpr35	UTSW	1	92,910,870 (GRCm39)	missense	probably damaging	0.98
R1515:Gpr35	UTSW	1	92,910,770 (GRCm39)	missense	probably damaging	1.00
R2872:Gpr35	UTSW	1	92,910,848 (GRCm39)	missense	probably benign	0.34
R2872:Gpr35	UTSW	1	92,910,848 (GRCm39)	missense	probably benign	0.34
R3498:Gpr35	UTSW	1	92,911,113 (GRCm39)	missense	probably damaging	1.00
R4732:Gpr35	UTSW	1	92,911,107 (GRCm39)	missense	probably damaging	1.00
R4733:Gpr35	UTSW	1	92,911,107 (GRCm39)	missense	probably damaging	1.00
R5270:Gpr35	UTSW	1	92,910,299 (GRCm39)	missense	probably benign	0.00
R5969:Gpr35	UTSW	1	92,910,942 (GRCm39)	missense	probably damaging	1.00
R6769:Gpr35	UTSW	1	92,910,426 (GRCm39)	missense	probably damaging	0.97
R6771:Gpr35	UTSW	1	92,910,426 (GRCm39)	missense	probably damaging	0.97
R7144:Gpr35	UTSW	1	92,910,353 (GRCm39)	missense	probably benign	0.11
R7395:Gpr35	UTSW	1	92,910,929 (GRCm39)	missense	probably damaging	1.00
R8156:Gpr35	UTSW	1	92,910,437 (GRCm39)	missense	probably damaging	1.00
Z1177:Gpr35	UTSW	1	92,910,738 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- ACATGGTCTGGGCCAACTTG -3'
(R):5'- AGATGCAGCCTCTCTAGTCCAAC -3'

Sequencing Primer
(F):5'- CAACTTGGCTGTGTTTGTCATC -3'
(R):5'- TAGTCCAACTGGGTCTCCTAGAG -3'

Posted On

2015-04-02