Mutagenetix > Incidental Mutation

Incidental Mutation 'R3804:Mslnl'

274526

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

MMRRC Submission

040879-MU

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3804 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

25736040-25748330 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 25742934 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Methionine at position 128 (V128M)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098]

AlphaFold

Q8C160

Predicted Effect

probably damaging
Transcript: ENSMUST00000047098
AA Change: V128M

PolyPhen 2 Score 0.971 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: V128M

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102319

Meta Mutation Damage Score

0.2148

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.5%
20x: 95.8%

Validation Efficiency

100% (67/67)

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700011L22Rik	C	T	8: 79,248,293	E54K	probably benign	Het
2610301B20Rik	A	G	4: 10,898,014	T199A	probably benign	Het
Adgra1	A	G	7: 139,845,594	T8A	probably benign	Het
Alms1	A	G	6: 85,619,647	Y954C	probably damaging	Het
AU021092	A	C	16: 5,216,762	F199V	possibly damaging	Het
Bahcc1	C	T	11: 120,283,358	P1648L	probably benign	Het
Cacna1s	T	A	1: 136,107,018	C1319S	possibly damaging	Het
Capn13	G	A	17: 73,339,401	P339L	probably benign	Het
Ccdc39	A	T	3: 33,819,895	M596K	probably damaging	Het
Cntn5	A	G	9: 9,781,663		probably benign	Het
Cyth4	A	G	15: 78,609,802	K159E	probably damaging	Het
Dgkz	C	A	2: 91,939,630	R563L	probably benign	Het
Dnah8	A	G	17: 30,670,647	E718G	probably benign	Het
Dopey1	T	C	9: 86,520,995	L1416P	probably damaging	Het
Dstyk	G	A	1: 132,449,726	A110T	probably damaging	Het
Eif1	A	G	11: 100,320,824	K95E	probably damaging	Het
Espnl	A	G	1: 91,322,221	D30G	probably benign	Het
Gast	T	C	11: 100,336,810	S73P	probably damaging	Het
Gmppb	T	C	9: 108,050,574	Y176H	probably damaging	Het
Grap2	A	G	15: 80,623,746	T4A	possibly damaging	Het
Grm8	T	G	6: 28,125,636	N164H	possibly damaging	Het
Gstm3	G	A	3: 107,964,235	T210I	probably benign	Het
Gtf3c3	A	G	1: 54,424,007		probably null	Het
Hmcn2	A	G	2: 31,352,885		probably null	Het
Icam2	A	G	11: 106,380,822	L94P	probably damaging	Het
Iqcm	C	A	8: 75,669,393	T188K	possibly damaging	Het
Jarid2	T	A	13: 44,902,831	N365K	probably benign	Het
Kank4	A	G	4: 98,780,133	S26P	probably damaging	Het
Lgr4	A	G	2: 110,008,197	K498E	probably benign	Het
Meltf	A	G	16: 31,884,998	H181R	probably benign	Het
Nf1	A	G	11: 79,559,521	D511G	probably null	Het
Nhlrc3	A	G	3: 53,458,631	V147A	possibly damaging	Het
Nrap	C	T	19: 56,321,779	D1595N	probably damaging	Het
Olfr1	T	C	11: 73,395,950	Q24R	probably benign	Het
Olfr430	T	A	1: 174,069,908	N203K	probably damaging	Het
Olfr481	A	T	7: 108,081,171	I126F	probably damaging	Het
Olfr805	T	A	10: 129,723,049	D165V	possibly damaging	Het
Pak3	G	A	X: 143,709,731	V87I	probably damaging	Het
Pgm2l1	T	C	7: 100,252,267	V121A	probably benign	Het
Phf19	A	G	2: 34,899,658	L350P	probably damaging	Het
Phf8	T	C	X: 151,572,576	S512P	possibly damaging	Het
Phkb	G	T	8: 85,922,229	E225*	probably null	Het
Pif1	T	A	9: 65,588,306	V166E	probably damaging	Het
Plaa	T	C	4: 94,569,888	D615G	probably damaging	Het
Prpf4b	T	C	13: 34,883,682		probably benign	Het
Rxra	T	C	2: 27,756,260	C374R	probably damaging	Het
Sept3	T	C	15: 82,286,429		probably benign	Het
Skint4	G	T	4: 112,118,181	V113L	probably damaging	Het
Slc16a10	G	C	10: 40,056,624	H314D	possibly damaging	Het
Slc22a15	C	T	3: 101,897,274	G145D	probably damaging	Het
Slc28a1	A	T	7: 81,126,221	I222F	probably damaging	Het
Slc43a2	T	C	11: 75,563,598	L323P	probably benign	Het
Sorbs3	A	G	14: 70,199,351		probably benign	Het
Spink10	C	T	18: 62,653,414		probably benign	Het
Suclg2	A	T	6: 95,497,668	I372N	probably damaging	Het
Sun1	C	A	5: 139,225,362	C164*	probably null	Het
Tax1bp1	T	C	6: 52,742,785	F453L	probably benign	Het
Tmem54	A	G	4: 129,108,220	N9S	probably benign	Het
Tmx4	A	G	2: 134,620,577	W145R	probably damaging	Het
Top1	A	G	2: 160,702,768	H268R	probably damaging	Het
Ttn	A	G	2: 76,810,731	L13598P	probably damaging	Het
Vmn1r40	A	G	6: 89,715,009	I269M	probably benign	Het
Wdr7	G	T	18: 63,720,836	R80L	probably benign	Het
Zap70	A	T	1: 36,771,142	Q111L	possibly damaging	Het
Zfp808	T	A	13: 62,172,083	H375Q	probably damaging	Het
Zkscan2	A	T	7: 123,495,142		probably benign	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25743667	unclassified	probably benign
IGL01629:Mslnl	APN	17	25744775	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25746151	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25747998	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25744103	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25744077	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25743203	nonsense	probably null
R0881:Mslnl	UTSW	17	25742965	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25743240	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25746181	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25744517	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25744969	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25742934	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25738978	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25738968	nonsense	probably null
R5257:Mslnl	UTSW	17	25746165	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25743159	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25737842	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25746775	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25737902	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25744557	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25746073	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25743212	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25743210	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25736921	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25743183	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25746777	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25746988	missense	probably benign
R8735:Mslnl	UTSW	17	25745088	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25745073	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25742720	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25742532	intron	probably benign
RF007:Mslnl	UTSW	17	25743228	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- GCCATCAGAAGGGAGGTTTG -3'
(R):5'- ACAGGTCTGAAGCACTGAGC -3'

Sequencing Primer
(F):5'- TTTGAACCCAGGACTGAGGTGAC -3'
(R):5'- GCCCTGCCGAGACAGTAG -3'

Posted On

2015-04-02