Mutagenetix > Incidental Mutation

Incidental Mutation 'R3849:Or7d10'

275824

Institutional Source

Beutler Lab

Gene Symbol

Or7d10

Ensembl Gene

ENSMUSG00000051118

Gene Name

olfactory receptor family 7 subfamily D member 10

Synonyms

18A, Olfr77, GA_x6K02T2PVTD-13660026-13660964, MOR143-1

MMRRC Submission

040897-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.076) question?

Stock #

R3849 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

19828525-19833806 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 19832105 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Phenylalanine at position 200 (Y200F)

Ref Sequence

ENSEMBL: ENSMUSP00000149055 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000057596] [ENSMUST00000217347]

AlphaFold

Q8VEY9

Predicted Effect

probably damaging
Transcript: ENSMUST00000057596
AA Change: Y200F

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000058810
Gene: ENSMUSG00000051118
AA Change: Y200F

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	3e-53	PFAM
Pfam:7tm_1	41	290	1.2e-24	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000217347
AA Change: Y200F

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.3%
3x: 98.8%
10x: 97.7%
20x: 96.1%

Validation Efficiency

100% (52/52)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9430038I01Rik	A	T	7: 136,978,069 (GRCm39)	N102K	possibly damaging	Het
Abca1	C	T	4: 53,061,481 (GRCm39)		probably benign	Het
Adamts17	T	A	7: 66,490,215 (GRCm39)	L99Q	possibly damaging	Het
Adamtsl1	A	T	4: 86,336,783 (GRCm39)	Q1564L	probably damaging	Het
Aspm	T	A	1: 139,386,024 (GRCm39)	V556E	probably benign	Het
Axin1	T	A	17: 26,406,771 (GRCm39)	Y455N	probably benign	Het
Cacna2d3	A	G	14: 29,069,077 (GRCm39)		probably null	Het
Car8	T	A	4: 8,189,353 (GRCm39)	I154F	probably benign	Het
Ccl27a	T	A	4: 41,773,232 (GRCm39)	T76S	probably benign	Het
Cep170	G	A	1: 176,583,409 (GRCm39)	A990V	probably benign	Het
Chd1	A	T	17: 15,952,133 (GRCm39)	T291S	probably damaging	Het
Col3a1	C	A	1: 45,361,150 (GRCm39)	P112T	unknown	Het
Cpa4	T	C	6: 30,590,872 (GRCm39)	F390S	probably damaging	Het
Cul5	T	G	9: 53,529,286 (GRCm39)	M800L	probably benign	Het
Dst	C	T	1: 34,251,400 (GRCm39)	S4165F	probably damaging	Het
Dusp8	T	C	7: 141,643,802 (GRCm39)	E37G	probably damaging	Het
Efr3b	T	C	12: 4,033,414 (GRCm39)	N131S	probably benign	Het
Fan1	T	C	7: 64,022,119 (GRCm39)	Y378C	probably damaging	Het
Fcgr2b	T	C	1: 170,795,704 (GRCm39)	N75S	possibly damaging	Het
Foxp4	A	G	17: 48,186,453 (GRCm39)	I442T	unknown	Het
Gp9	A	G	6: 87,756,133 (GRCm39)	I49M	probably benign	Het
Grip1	G	A	10: 119,765,863 (GRCm39)	G65D	probably damaging	Het
Hoxc10	T	C	15: 102,875,879 (GRCm39)	V196A	probably benign	Het
Hpx	A	G	7: 105,245,498 (GRCm39)	C92R	probably damaging	Het
Ighv1-22	A	G	12: 114,710,301 (GRCm39)	F9S	possibly damaging	Het
Kif24	C	T	4: 41,404,734 (GRCm39)	R422H	probably damaging	Het
Klk1b1	T	C	7: 43,618,751 (GRCm39)	Y43H	probably damaging	Het
Lmo7	A	G	14: 102,159,531 (GRCm39)		probably null	Het
Lrguk	A	G	6: 34,050,703 (GRCm39)	D387G	probably damaging	Het
Lysmd1	G	A	3: 95,045,772 (GRCm39)	G203D	probably damaging	Het
Mpnd	T	C	17: 56,318,692 (GRCm39)	S150P	probably damaging	Het
Mrc2	T	C	11: 105,183,729 (GRCm39)		probably null	Het
Mtr	A	G	13: 12,262,251 (GRCm39)	S152P	probably benign	Het
Naip2	A	T	13: 100,315,940 (GRCm39)	L280Q	probably damaging	Het
Naip2	G	C	13: 100,315,941 (GRCm39)	L280V	probably damaging	Het
Ndufa13	T	C	8: 70,354,260 (GRCm39)	D9G	probably damaging	Het
Nek1	T	A	8: 61,525,349 (GRCm39)	F596I	probably damaging	Het
Nsd1	A	G	13: 55,394,504 (GRCm39)	T702A	probably benign	Het
Pam	T	A	1: 97,782,481 (GRCm39)		probably benign	Het
Plxna1	C	A	6: 89,333,501 (GRCm39)	R376L	probably damaging	Het
Plxnc1	A	G	10: 94,630,294 (GRCm39)	V1535A	probably benign	Het
Prdx5	A	G	19: 6,884,218 (GRCm39)	L166P	probably damaging	Het
Prol1	A	T	5: 88,476,476 (GRCm39)	I289F	unknown	Het
Prss23	T	C	7: 89,158,959 (GRCm39)	Y370C	probably damaging	Het
Rimbp3	A	T	16: 17,028,163 (GRCm39)	H529L	probably benign	Het
Rnase2b	T	A	14: 51,400,205 (GRCm39)	H95Q	probably damaging	Het
Rnf103	C	G	6: 71,485,859 (GRCm39)	C163W	probably damaging	Het
Rnf17	T	C	14: 56,749,753 (GRCm39)	V1433A	probably damaging	Het
Rusf1	C	T	7: 127,884,380 (GRCm39)	V201I	probably damaging	Het
Syne2	C	T	12: 76,092,839 (GRCm39)	Q5150*	probably null	Het
Tas2r110	T	A	6: 132,845,638 (GRCm39)	I223N	probably damaging	Het
Tead2	T	A	7: 44,881,752 (GRCm39)		probably null	Het
Vwa3a	A	T	7: 120,361,687 (GRCm39)	K133*	probably null	Het
Vwce	T	C	19: 10,624,269 (GRCm39)	S387P	probably damaging	Het
Zbtb41	T	A	1: 139,351,734 (GRCm39)	H282Q	probably benign	Het

Other mutations in Or7d10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01152:Or7d10	APN	9	19,832,245 (GRCm39)	missense	possibly damaging	0.87
IGL01318:Or7d10	APN	9	19,832,054 (GRCm39)	missense	probably benign	0.44
IGL01547:Or7d10	APN	9	19,832,197 (GRCm39)	missense	probably benign	0.00
IGL01635:Or7d10	APN	9	19,831,780 (GRCm39)	missense	probably damaging	1.00
IGL02112:Or7d10	APN	9	19,831,821 (GRCm39)	missense	possibly damaging	0.48
IGL02858:Or7d10	APN	9	19,831,747 (GRCm39)	missense	probably damaging	0.99
IGL02904:Or7d10	APN	9	19,832,393 (GRCm39)	missense	probably damaging	1.00
IGL02956:Or7d10	APN	9	19,832,348 (GRCm39)	missense	possibly damaging	0.87
IGL03066:Or7d10	APN	9	19,831,667 (GRCm39)	missense	probably benign	0.12
R0662:Or7d10	UTSW	9	19,831,796 (GRCm39)	missense	probably damaging	1.00
R1222:Or7d10	UTSW	9	19,832,344 (GRCm39)	missense	possibly damaging	0.87
R1572:Or7d10	UTSW	9	19,832,208 (GRCm39)	missense	probably benign	0.35
R1761:Or7d10	UTSW	9	19,832,445 (GRCm39)	makesense	probably null
R2409:Or7d10	UTSW	9	19,832,077 (GRCm39)	missense	probably damaging	1.00
R2409:Or7d10	UTSW	9	19,832,072 (GRCm39)	missense	probably benign	0.31
R3621:Or7d10	UTSW	9	19,832,209 (GRCm39)	missense	probably damaging	0.99
R3850:Or7d10	UTSW	9	19,832,105 (GRCm39)	missense	probably damaging	1.00
R4277:Or7d10	UTSW	9	19,831,685 (GRCm39)	missense	possibly damaging	0.91
R4768:Or7d10	UTSW	9	19,831,841 (GRCm39)	missense	possibly damaging	0.56
R4979:Or7d10	UTSW	9	19,831,655 (GRCm39)	missense	probably benign	0.03
R5276:Or7d10	UTSW	9	19,831,917 (GRCm39)	missense	possibly damaging	0.87
R5503:Or7d10	UTSW	9	19,831,675 (GRCm39)	missense	probably benign	0.36
R5760:Or7d10	UTSW	9	19,832,050 (GRCm39)	missense	probably benign	0.00
R5778:Or7d10	UTSW	9	19,832,337 (GRCm39)	missense	probably benign	0.20
R5930:Or7d10	UTSW	9	19,832,206 (GRCm39)	missense	probably damaging	0.99
R6012:Or7d10	UTSW	9	19,832,237 (GRCm39)	missense	probably damaging	0.99
R7269:Or7d10	UTSW	9	19,831,631 (GRCm39)	missense	possibly damaging	0.95
R7977:Or7d10	UTSW	9	19,831,610 (GRCm39)	missense	possibly damaging	0.48
R7987:Or7d10	UTSW	9	19,831,610 (GRCm39)	missense	possibly damaging	0.48
R8174:Or7d10	UTSW	9	19,832,020 (GRCm39)	missense	probably damaging	1.00
Z1088:Or7d10	UTSW	9	19,832,008 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATGAACCCTCGAATCTGTGGC -3'
(R):5'- TGGATGAATGGGCTACAGC -3'

Sequencing Primer
(F):5'- CTCGAATCTGTGGCCTTCTGG -3'
(R):5'- TGAGATGAACTACAAATCCTGTCC -3'

Posted On

2015-04-06