Mutagenetix > Incidental Mutation

Incidental Mutation 'R3852:Tas2r104'

275968

Institutional Source

Beutler Lab

Gene Symbol

Tas2r104

Ensembl Gene

ENSMUSG00000061977

Gene Name

taste receptor, type 2, member 104

Synonyms

T2R04, mGR04, Tas2r4, mt2r45

MMRRC Submission

040784-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.052) question?

Stock #

R3852 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

131684836-131685744 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 131684925 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 274 (C274S)

Ref Sequence

ENSEMBL: ENSMUSP00000072237 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053652] [ENSMUST00000072404] [ENSMUST00000080619]

AlphaFold

Q7M723

Predicted Effect

probably benign
Transcript: ENSMUST00000053652

SMART Domains

Protein: ENSMUSP00000058006
Gene: ENSMUSG00000051153

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	298	9.4e-109	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000072404
AA Change: C274S

PolyPhen 2 Score 0.094 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000072237
Gene: ENSMUSG00000061977
AA Change: C274S

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	298	8.3e-102	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000080619

SMART Domains

Protein: ENSMUSP00000079453
Gene: ENSMUSG00000063478

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	298	8.1e-104	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.5%
20x: 95.7%

Validation Efficiency

96% (45/47)

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcf3	T	C	16: 20,560,439	V685A	probably damaging	Het
Abhd4	T	C	14: 54,265,367	Y44H	probably damaging	Het
AI429214	A	G	8: 36,994,442	D248G	probably damaging	Het
AY358078	A	G	14: 51,805,553	T233A	unknown	Het
Calcr	T	C	6: 3,693,735	Y353C	probably damaging	Het
Ccdc34	A	G	2: 110,032,428	K193E	possibly damaging	Het
Dhcr24	A	G	4: 106,573,873	E253G	probably benign	Het
Dnah6	A	G	6: 73,127,927	V1841A	possibly damaging	Het
Dpp10	A	T	1: 123,485,924	Y186*	probably null	Het
Dpysl4	C	T	7: 139,100,935	T575M	probably damaging	Het
Dusp7	T	A	9: 106,373,893	S406T	probably benign	Het
Gga3	T	A	11: 115,587,542	T475S	probably benign	Het
Gm10271	T	A	10: 116,956,874	K36*	probably null	Het
Gm572	G	A	4: 148,668,872	E325K	possibly damaging	Het
Golga2	A	G	2: 32,305,611	E806G	probably benign	Het
Igf1	A	T	10: 87,915,319	K126*	probably null	Het
Itpkc	A	T	7: 27,227,612	N292K	probably benign	Het
Klhl1	A	T	14: 96,280,205	M345K	probably benign	Het
Lrp2	A	G	2: 69,537,565	V201A	probably damaging	Het
Lrrc8a	C	T	2: 30,261,960	T757M	probably benign	Het
Mios	T	A	6: 8,216,453	I459K	probably benign	Het
Mrps11	T	C	7: 78,790,645	I94T	probably damaging	Het
Ms4a4c	T	A	19: 11,416,395	S68T	probably benign	Het
Mstn	C	T	1: 53,061,971	T69I	possibly damaging	Het
Myo10	A	G	15: 25,779,626	K28E	probably damaging	Het
Nol6	A	G	4: 41,117,452	S914P	probably damaging	Het
Ntn1	C	G	11: 68,385,793	D110H	probably damaging	Het
Olfr547	C	A	7: 102,535,280	H178N	probably benign	Het
Olfr585	T	C	7: 103,098,184	S148P	probably damaging	Het
Pcdh17	C	T	14: 84,447,259	Q389*	probably null	Het
Pik3r2	G	A	8: 70,770,421	R452C	probably benign	Het
Prr14l	T	C	5: 32,830,345	E602G	probably damaging	Het
Rusc2	C	A	4: 43,416,424	Q577K	probably benign	Het
Sacm1l	T	A	9: 123,587,576	M534K	probably damaging	Het
Shroom3	G	T	5: 92,943,086	V1151F	probably damaging	Het
Slco4a1	C	T	2: 180,464,091	T22M	probably benign	Het
Svs1	C	T	6: 48,987,994	P312L	possibly damaging	Het
Tnrc6c	C	T	11: 117,723,529	R838W	probably damaging	Het
Trim43c	T	A	9: 88,840,401	H33Q	probably damaging	Het
Ttc17	A	T	2: 94,369,413	I411K	possibly damaging	Het
Ttn	G	T	2: 76,751,334	L23072I	possibly damaging	Het
Ttn	A	C	2: 76,955,024	V669G	possibly damaging	Het
Vmn1r60	A	T	7: 5,545,027	F25I	possibly damaging	Het
Vmn2r9	A	G	5: 108,848,131	I217T	probably damaging	Het

Other mutations in Tas2r104

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00590:Tas2r104	APN	6	131685567	missense	probably damaging	1.00
IGL01390:Tas2r104	APN	6	131685485	missense	probably benign	0.13
IGL02751:Tas2r104	APN	6	131685144	missense	probably damaging	1.00
PIT4585001:Tas2r104	UTSW	6	131685558	missense	possibly damaging	0.50
R0218:Tas2r104	UTSW	6	131685092	missense	probably damaging	1.00
R0453:Tas2r104	UTSW	6	131685341	missense	probably benign	0.00
R0472:Tas2r104	UTSW	6	131685471	missense	probably benign	0.06
R0614:Tas2r104	UTSW	6	131685202	missense	probably damaging	1.00
R1290:Tas2r104	UTSW	6	131684845	nonsense	probably null
R1480:Tas2r104	UTSW	6	131685294	missense	probably benign	0.17
R1698:Tas2r104	UTSW	6	131685584	missense	probably damaging	1.00
R2050:Tas2r104	UTSW	6	131685120	missense	probably damaging	1.00
R2229:Tas2r104	UTSW	6	131685132	missense	probably damaging	1.00
R3824:Tas2r104	UTSW	6	131685039	missense	possibly damaging	0.55
R4283:Tas2r104	UTSW	6	131685411	missense	probably damaging	1.00
R4583:Tas2r104	UTSW	6	131685435	missense	probably benign	0.00
R4710:Tas2r104	UTSW	6	131685444	missense	probably damaging	0.96
R4954:Tas2r104	UTSW	6	131685005	missense	probably damaging	0.99
R5559:Tas2r104	UTSW	6	131685131	missense	probably damaging	1.00
R5765:Tas2r104	UTSW	6	131685273	missense	probably benign
R5843:Tas2r104	UTSW	6	131684975	missense	probably damaging	0.99
R7304:Tas2r104	UTSW	6	131685042	missense	possibly damaging	0.90
R8559:Tas2r104	UTSW	6	131685425	missense	probably damaging	1.00
R8864:Tas2r104	UTSW	6	131685669	missense	possibly damaging	0.95
R8951:Tas2r104	UTSW	6	131685606	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCAGTCAATCAGTTGCCAAAG -3'
(R):5'- TCAGGATTCAGAGATCTCAACAC -3'

Sequencing Primer
(F):5'- CAGTCAATCAGTTGCCAAAGGTTTC -3'
(R):5'- GGATTCAGAGATCTCAACACAGAAGC -3'

Posted On

2015-04-06