Mutagenetix > Incidental Mutation

Incidental Mutation 'R3870:Usp32'

276525

Institutional Source

Beutler Lab

Gene Symbol

Usp32

Ensembl Gene

ENSMUSG00000000804

Gene Name

ubiquitin specific peptidase 32

Synonyms

6430526O11Rik, 2900074J03Rik

MMRRC Submission

040789-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R3870 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

84984442-85140161 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 85007055 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 1153 (Y1153*)

Ref Sequence

ENSEMBL: ENSMUSP00000103710 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000108075]

AlphaFold

F8VPZ3

Predicted Effect

probably benign
Transcript: ENSMUST00000000821

SMART Domains

Protein: ENSMUSP00000000821
Gene: ENSMUSG00000000804

Domain	Start	End	E-Value	Type
Pfam:UCH	32	260	4.1e-51	PFAM
Pfam:UCH_1	33	228	1.7e-7	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000108075
AA Change: Y1153*

SMART Domains

Protein: ENSMUSP00000103710
Gene: ENSMUSG00000000804
AA Change: Y1153*

Domain	Start	End	E-Value	Type
EFh	232	260	4.66e0	SMART
EFh	268	296	5.8e-1	SMART
Blast:EFh	318	346	5e-7	BLAST
DUSP	389	588	2.32e-16	SMART
Pfam:Ubiquitin_3	628	711	2.4e-9	PFAM
Pfam:UCH	733	1564	2.4e-83	PFAM
Pfam:UCH_1	1202	1547	2.9e-12	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.6%
20x: 96.1%

Validation Efficiency

90% (61/68)

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700028J19Rik	T	A	7: 44,231,404 (GRCm38)		probably null	Het
2410089E03Rik	A	T	15: 8,218,464 (GRCm38)	K1499M	probably damaging	Het
Adam22	C	A	5: 8,132,418 (GRCm38)	C514F	probably damaging	Het
Akap8	G	A	17: 32,317,839 (GRCm38)		probably benign	Het
Armcx2	A	T	X: 134,806,299 (GRCm38)	V195E	probably benign	Het
Atg7	T	C	6: 114,697,047 (GRCm38)	S301P	possibly damaging	Het
Cc2d2a	C	A	5: 43,718,691 (GRCm38)	Y1003*	probably null	Het
Ccdc93	A	G	1: 121,463,114 (GRCm38)	S272G	probably benign	Het
Ces1d	C	G	8: 93,175,086 (GRCm38)	L418F	probably benign	Het
Cntnap5a	A	G	1: 116,060,249 (GRCm38)	E170G	probably damaging	Het
Crygs	C	T	16: 22,805,551 (GRCm38)	G102D	possibly damaging	Het
Ehd4	T	A	2: 120,136,953 (GRCm38)	D120V	probably damaging	Het
Eif4g3	T	A	4: 138,096,900 (GRCm38)	V71E	probably damaging	Het
Exoc5	A	G	14: 49,019,396 (GRCm38)		probably benign	Het
Glud1	T	A	14: 34,325,580 (GRCm38)		probably benign	Het
Gm10985	TTCTCTCTCTCTCTCTCT	TTCTCTCTCTCTCTCT	3: 53,845,205 (GRCm38)		probably null	Het
Gm11555	G	T	11: 99,649,990 (GRCm38)	C64*	probably null	Het
Gm5468	A	G	15: 25,414,475 (GRCm38)		probably benign	Het
Gpatch2	T	A	1: 187,322,294 (GRCm38)	L74Q	probably damaging	Het
Hnrnpa0	G	A	13: 58,127,899 (GRCm38)	R139C	probably damaging	Het
Hrh1	A	G	6: 114,480,919 (GRCm38)	Y387C	probably damaging	Het
Ldb3	A	T	14: 34,567,483 (GRCm38)	D216E	probably damaging	Het
Lingo1	A	T	9: 56,619,725 (GRCm38)	S533T	probably benign	Het
Lmtk2	T	G	5: 144,166,427 (GRCm38)		probably benign	Het
Map1s	A	G	8: 70,917,101 (GRCm38)	E939G	possibly damaging	Het
Mast1	G	A	8: 84,918,731 (GRCm38)	T695I	probably damaging	Het
Mettl21e	G	A	1: 44,206,364 (GRCm38)	R241W	probably benign	Het
Mfsd4a	G	A	1: 132,046,353 (GRCm38)	T261I	probably damaging	Het
Mmel1	T	C	4: 154,883,638 (GRCm38)	S144P	probably benign	Het
Myo16	A	T	8: 10,442,239 (GRCm38)	H727L	probably benign	Het
Ncoa6	T	C	2: 155,415,557 (GRCm38)		probably null	Het
Nipa2	T	C	7: 55,932,942 (GRCm38)	R352G	probably damaging	Het
Oaf	C	T	9: 43,222,758 (GRCm38)	R222Q	probably benign	Het
Olfr701	T	A	7: 106,818,840 (GRCm38)	Y252*	probably null	Het
Pard3	T	C	8: 127,409,686 (GRCm38)	S847P	probably damaging	Het
Pgbd1	C	T	13: 21,434,370 (GRCm38)	R39H	possibly damaging	Het
Plcb1	A	C	2: 135,325,671 (GRCm38)	I462L	probably damaging	Het
Prex2	T	C	1: 11,160,192 (GRCm38)	V814A	possibly damaging	Het
Rasal3	G	A	17: 32,393,548 (GRCm38)	R780W	possibly damaging	Het
Rubcnl	C	T	14: 75,040,916 (GRCm38)	P380L	probably benign	Het
Ryk	A	G	9: 102,891,228 (GRCm38)	E359G	probably damaging	Het
Sall2	C	A	14: 52,313,994 (GRCm38)	L579F	probably damaging	Het
Satb2	A	G	1: 56,891,220 (GRCm38)	S215P	probably damaging	Het
Scg3	T	C	9: 75,675,499 (GRCm38)		probably benign	Het
Slc35f5	A	G	1: 125,562,361 (GRCm38)	T65A	probably benign	Het
Snx33	T	C	9: 56,926,740 (GRCm38)	N15S	probably benign	Het
Stat2	T	A	10: 128,277,893 (GRCm38)	S180R	probably benign	Het
Stxbp2	A	G	8: 3,634,079 (GRCm38)	T129A	probably damaging	Het
Tas1r3	A	T	4: 155,861,353 (GRCm38)	C529S	probably damaging	Het
Tlr12	T	A	4: 128,616,568 (GRCm38)	M630L	probably benign	Het
Tnfrsf10b	T	G	14: 69,773,456 (GRCm38)	D103E	probably benign	Het
Togaram1	A	C	12: 65,002,645 (GRCm38)	E1285D	probably benign	Het
Tppp	A	G	13: 74,030,772 (GRCm38)	T111A	probably benign	Het
Trim12c	T	C	7: 104,348,337 (GRCm38)	Q4R	probably benign	Het
Ttbk2	T	G	2: 120,740,019 (GRCm38)	S1149R	probably damaging	Het
Uncx	T	C	5: 139,547,365 (GRCm38)	L395P	probably damaging	Het
Usp14	A	G	18: 10,002,370 (GRCm38)	S314P	possibly damaging	Het
Vmn2r6	T	C	3: 64,556,621 (GRCm38)	E264G	probably damaging	Het
Vmn2r77	T	C	7: 86,811,842 (GRCm38)	F792S	probably damaging	Het
Vps13c	A	G	9: 67,884,726 (GRCm38)	I425V	probably benign	Het
Vstm4	C	T	14: 32,863,755 (GRCm38)	A93V	probably benign	Het
Xrcc6	T	A	15: 82,025,684 (GRCm38)	S97T	probably benign	Het
Zscan20	A	G	4: 128,586,425 (GRCm38)	C758R	probably damaging	Het

Other mutations in Usp32

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00529:Usp32	APN	11	84,994,426 (GRCm38)	missense	probably damaging	1.00
IGL00701:Usp32	APN	11	85,059,125 (GRCm38)	splice site	probably null
IGL00848:Usp32	APN	11	85,051,181 (GRCm38)	splice site	probably benign
IGL00934:Usp32	APN	11	85,007,076 (GRCm38)	missense	probably damaging	1.00
IGL01019:Usp32	APN	11	85,039,265 (GRCm38)	missense	probably damaging	0.97
IGL01302:Usp32	APN	11	84,988,482 (GRCm38)	missense	probably benign	0.05
IGL01444:Usp32	APN	11	85,059,164 (GRCm38)	missense	probably damaging	0.97
IGL01575:Usp32	APN	11	85,022,802 (GRCm38)	missense	probably damaging	1.00
IGL01981:Usp32	APN	11	85,036,524 (GRCm38)	missense	probably benign	0.02
IGL02118:Usp32	APN	11	85,032,177 (GRCm38)	nonsense	probably null
IGL02159:Usp32	APN	11	85,005,802 (GRCm38)	splice site	probably null
IGL02227:Usp32	APN	11	84,986,481 (GRCm38)	missense	probably damaging	1.00
IGL02363:Usp32	APN	11	85,044,787 (GRCm38)	missense	probably benign	0.01
IGL02524:Usp32	APN	11	85,010,011 (GRCm38)	nonsense	probably null
IGL02613:Usp32	APN	11	85,040,070 (GRCm38)	missense	probably damaging	0.99
IGL02720:Usp32	APN	11	85,006,991 (GRCm38)	critical splice donor site	probably null
IGL02738:Usp32	APN	11	85,083,806 (GRCm38)	missense	probably damaging	1.00
IGL02929:Usp32	APN	11	84,988,372 (GRCm38)	missense	probably benign	0.01
IGL03303:Usp32	APN	11	85,022,832 (GRCm38)	missense	probably damaging	1.00
BB010:Usp32	UTSW	11	85,007,059 (GRCm38)	missense	probably damaging	1.00
BB020:Usp32	UTSW	11	85,007,059 (GRCm38)	missense	probably damaging	1.00
PIT4812001:Usp32	UTSW	11	85,010,074 (GRCm38)	missense	probably damaging	1.00
R0026:Usp32	UTSW	11	85,032,074 (GRCm38)	missense	possibly damaging	0.48
R0295:Usp32	UTSW	11	85,053,692 (GRCm38)	missense	probably damaging	0.98
R1320:Usp32	UTSW	11	85,017,793 (GRCm38)	missense	probably damaging	0.98
R1712:Usp32	UTSW	11	85,042,580 (GRCm38)	missense	probably benign	0.12
R1922:Usp32	UTSW	11	85,007,004 (GRCm38)	nonsense	probably null
R1973:Usp32	UTSW	11	85,103,931 (GRCm38)	missense	probably benign	0.09
R2010:Usp32	UTSW	11	85,040,004 (GRCm38)	missense	probably damaging	0.98
R2082:Usp32	UTSW	11	85,030,512 (GRCm38)	missense	probably damaging	0.99
R2355:Usp32	UTSW	11	85,005,909 (GRCm38)	missense	probably benign	0.34
R3147:Usp32	UTSW	11	85,029,087 (GRCm38)	missense	probably damaging	1.00
R3160:Usp32	UTSW	11	85,025,536 (GRCm38)	missense	probably damaging	0.97
R3162:Usp32	UTSW	11	85,025,536 (GRCm38)	missense	probably damaging	0.97
R3716:Usp32	UTSW	11	85,042,563 (GRCm38)	missense	probably damaging	1.00
R3816:Usp32	UTSW	11	84,994,384 (GRCm38)	critical splice donor site	probably null
R3871:Usp32	UTSW	11	85,081,156 (GRCm38)	missense	probably null	0.81
R4041:Usp32	UTSW	11	85,017,739 (GRCm38)	missense	probably benign	0.40
R4079:Usp32	UTSW	11	85,039,229 (GRCm38)	missense	probably damaging	0.98
R4332:Usp32	UTSW	11	85,103,978 (GRCm38)	missense	possibly damaging	0.79
R4396:Usp32	UTSW	11	85,053,975 (GRCm38)	missense	probably benign
R4580:Usp32	UTSW	11	85,059,127 (GRCm38)	critical splice donor site	probably null
R4620:Usp32	UTSW	11	85,059,127 (GRCm38)	critical splice donor site	probably null
R4744:Usp32	UTSW	11	84,994,393 (GRCm38)	missense	probably damaging	1.00
R4909:Usp32	UTSW	11	85,055,772 (GRCm38)	nonsense	probably null
R5056:Usp32	UTSW	11	85,026,795 (GRCm38)	missense	probably benign	0.07
R5111:Usp32	UTSW	11	85,077,331 (GRCm38)	missense	possibly damaging	0.95
R5213:Usp32	UTSW	11	85,022,259 (GRCm38)	missense	probably damaging	1.00
R5308:Usp32	UTSW	11	85,017,718 (GRCm38)	missense	probably benign	0.12
R5381:Usp32	UTSW	11	85,059,127 (GRCm38)	critical splice donor site	probably benign
R5538:Usp32	UTSW	11	85,017,786 (GRCm38)	missense	possibly damaging	0.65
R5659:Usp32	UTSW	11	85,077,414 (GRCm38)	missense	possibly damaging	0.94
R6006:Usp32	UTSW	11	84,992,451 (GRCm38)	critical splice donor site	probably null
R6011:Usp32	UTSW	11	85,032,097 (GRCm38)	missense	possibly damaging	0.70
R6029:Usp32	UTSW	11	85,025,582 (GRCm38)	missense	probably damaging	0.99
R6074:Usp32	UTSW	11	84,994,573 (GRCm38)	missense	probably benign	0.00
R6331:Usp32	UTSW	11	84,986,576 (GRCm38)	missense	possibly damaging	0.92
R6353:Usp32	UTSW	11	85,022,281 (GRCm38)	missense	probably benign
R6714:Usp32	UTSW	11	85,026,870 (GRCm38)	missense	probably damaging	0.99
R6778:Usp32	UTSW	11	85,025,686 (GRCm38)	missense	probably benign	0.00
R6988:Usp32	UTSW	11	85,010,143 (GRCm38)	missense	probably benign	0.35
R6992:Usp32	UTSW	11	85,032,088 (GRCm38)	missense	probably damaging	0.99
R7182:Usp32	UTSW	11	85,040,170 (GRCm38)	missense	probably benign	0.34
R7186:Usp32	UTSW	11	85,051,234 (GRCm38)	missense	probably benign	0.45
R7198:Usp32	UTSW	11	85,022,855 (GRCm38)	frame shift	probably null
R7201:Usp32	UTSW	11	85,022,855 (GRCm38)	frame shift	probably null
R7469:Usp32	UTSW	11	84,988,553 (GRCm38)	missense	possibly damaging	0.94
R7502:Usp32	UTSW	11	85,022,898 (GRCm38)	missense	possibly damaging	0.48
R7513:Usp32	UTSW	11	85,027,112 (GRCm38)	nonsense	probably null
R7629:Usp32	UTSW	11	85,019,855 (GRCm38)	frame shift	probably null
R7703:Usp32	UTSW	11	85,077,327 (GRCm38)	missense	probably damaging	0.99
R7741:Usp32	UTSW	11	84,987,281 (GRCm38)	missense	probably damaging	0.99
R7765:Usp32	UTSW	11	84,994,408 (GRCm38)	missense	probably damaging	1.00
R7933:Usp32	UTSW	11	85,007,059 (GRCm38)	missense	probably damaging	1.00
R7973:Usp32	UTSW	11	85,022,808 (GRCm38)	missense	probably damaging	0.99
R7989:Usp32	UTSW	11	85,034,300 (GRCm38)	missense
R7998:Usp32	UTSW	11	84,994,426 (GRCm38)	missense	probably damaging	1.00
R8292:Usp32	UTSW	11	85,077,401 (GRCm38)	missense	probably damaging	0.99
R8305:Usp32	UTSW	11	85,032,185 (GRCm38)	missense	possibly damaging	0.83
R8548:Usp32	UTSW	11	85,017,827 (GRCm38)	missense	possibly damaging	0.52
R8924:Usp32	UTSW	11	85,025,544 (GRCm38)	missense	probably damaging	0.98
R9002:Usp32	UTSW	11	85,053,951 (GRCm38)	missense	probably damaging	0.96
R9145:Usp32	UTSW	11	85,022,292 (GRCm38)	missense	probably damaging	1.00
R9209:Usp32	UTSW	11	85,040,012 (GRCm38)	missense	probably damaging	0.98
R9211:Usp32	UTSW	11	85,022,733 (GRCm38)	missense	probably damaging	1.00
R9296:Usp32	UTSW	11	85,017,652 (GRCm38)	missense	probably damaging	1.00
R9310:Usp32	UTSW	11	85,051,202 (GRCm38)	missense	probably benign	0.29
R9417:Usp32	UTSW	11	84,994,543 (GRCm38)	missense	probably damaging	1.00
R9514:Usp32	UTSW	11	85,022,734 (GRCm38)	missense	probably damaging	0.99
R9652:Usp32	UTSW	11	85,030,491 (GRCm38)	missense	probably damaging	0.97
R9723:Usp32	UTSW	11	85,044,710 (GRCm38)	nonsense	probably null
R9757:Usp32	UTSW	11	85,077,329 (GRCm38)	nonsense	probably null
X0028:Usp32	UTSW	11	84,992,606 (GRCm38)	missense	probably benign	0.05
Z1177:Usp32	UTSW	11	84,988,612 (GRCm38)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- CACAAAGAAGAGATGGACAATTCTC -3'
(R):5'- TTTGGGTTATACAGAAAGAGCTCC -3'

Sequencing Primer
(F):5'- GACTCTGTGAGACTGGTTT -3'
(R):5'- GTTATACAGAAAGAGCTCCTGTGCC -3'

Posted On

2015-04-06