Mutagenetix > Incidental Mutation

Incidental Mutation 'R3871:Olfr1025-ps1'

276552

Institutional Source

Beutler Lab

Gene Symbol

Olfr1025-ps1

Ensembl Gene

ENSMUSG00000058884

Gene Name

olfactory receptor 1025, pseudogene 1

Synonyms

MOR196-6_p, GA_x6K02T2Q125-47397266-47398205, MOR196-5P

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.105) question?

Stock #

R3871 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

85915193-85921381 bp(+) (GRCm38)

Type of Mutation

splice site

DNA Base Change (assembly)

T to G at 85918582 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000151927 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000056718] [ENSMUST00000188749] [ENSMUST00000213474] [ENSMUST00000217615] [ENSMUST00000219615]

AlphaFold

A0A140T8K3

Predicted Effect

probably null
Transcript: ENSMUST00000056718

SMART Domains

Protein: ENSMUSP00000049887
Gene: ENSMUSG00000042863

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	6.1e-58	PFAM
Pfam:7tm_1	41	290	9e-25	PFAM

Predicted Effect

SMART Domains

Protein: ENSMUSP00000076311
Gene: ENSMUSG00000058884
AA Change: I219S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.5e-57	PFAM
Pfam:7tm_1	41	290	2.6e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000188749
AA Change: I219S

PolyPhen 2 Score 0.029 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000139860
Gene: ENSMUSG00000058884
AA Change: I219S

Domain	Start	End	E-Value	Type
low complexity region	26	40	N/A	INTRINSIC
Pfam:7tm_1	41	290	5.7e-30	PFAM
Pfam:7tm_4	139	283	7.8e-48	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000213474
AA Change: I219S

PolyPhen 2 Score 0.097 (Sensitivity: 0.93; Specificity: 0.85)

Predicted Effect

probably null
Transcript: ENSMUST00000217615

Predicted Effect

probably null
Transcript: ENSMUST00000219615

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aldh1a1	T	A	19: 20,624,753	Y225*	probably null	Het
Bard1	T	C	1: 71,074,940	K294R	probably benign	Het
Bcap29	A	T	12: 31,617,081	S194T	probably benign	Het
Ccdc40	G	A	11: 119,264,281	V1116M	probably damaging	Het
Cntnap5a	A	G	1: 116,060,249	E170G	probably damaging	Het
Crygs	C	T	16: 22,805,551	G102D	possibly damaging	Het
Cyp2c54	T	C	19: 40,072,423	D92G	probably benign	Het
Dpp6	T	C	5: 27,469,465	F197L	probably benign	Het
Filip1l	G	T	16: 57,513,286	K147N	probably damaging	Het
Hrnr	T	A	3: 93,331,874	S3140T	unknown	Het
Igfn1	G	T	1: 135,968,836	H1331N	probably benign	Het
Kalrn	C	T	16: 34,203,856		probably null	Het
Kmt2d	TTGCTGCTGCTGCTGCTGCTGCTGC	TTGCTGCTGCTGCTGCTGC	15: 98,851,021		probably benign	Het
Mfng	A	G	15: 78,756,621	L308P	probably damaging	Het
Nt5e	C	A	9: 88,364,693	N327K	probably benign	Het
Pgbd1	C	T	13: 21,434,370	R39H	possibly damaging	Het
Phactr4	T	C	4: 132,377,249	T256A	probably benign	Het
Rab24	T	C	13: 55,321,179	D63G	probably damaging	Het
Rubcnl	C	T	14: 75,040,916	P380L	probably benign	Het
Satb2	A	G	1: 56,891,220	S215P	probably damaging	Het
Serpina3b	A	T	12: 104,138,788	I408F	probably damaging	Het
Setx	A	G	2: 29,145,741	D746G	probably damaging	Het
Sf3a2	A	C	10: 80,804,693		probably benign	Het
Snx33	T	C	9: 56,926,740	N15S	probably benign	Het
Snx9	C	T	17: 5,891,781	P61L	probably benign	Het
Sult2a6	T	C	7: 14,254,776	K20E	probably benign	Het
Tas2r122	A	G	6: 132,711,580	S117P	probably benign	Het
Tmem26	G	A	10: 68,778,732	E326K	probably benign	Het
Tnpo2	T	A	8: 85,054,751	C789S	probably null	Het
Togaram1	A	C	12: 65,002,645	E1285D	probably benign	Het
Ubxn7	T	A	16: 32,381,430	S335T	possibly damaging	Het
Unc119b	G	A	5: 115,130,508	T106M	probably damaging	Het
Usp14	A	G	18: 10,002,370	S314P	possibly damaging	Het
Usp32	T	C	11: 85,081,156	D129G	probably null	Het

Other mutations in Olfr1025-ps1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01660:Olfr1025-ps1	APN	2	85918564	missense	probably benign	0.25
IGL02881:Olfr1025-ps1	APN	2	85918116	missense	probably benign	0.00
R0097:Olfr1025-ps1	UTSW	2	85918840	missense	probably benign	0.00
R0097:Olfr1025-ps1	UTSW	2	85918840	missense	probably benign	0.00
R0324:Olfr1025-ps1	UTSW	2	85917951	missense	probably benign	0.22
R0392:Olfr1025-ps1	UTSW	2	85918762	missense	possibly damaging	0.81
R0765:Olfr1025-ps1	UTSW	2	85918705	missense	probably damaging	0.99
R1199:Olfr1025-ps1	UTSW	2	85918035	missense	probably benign	0.00
R1894:Olfr1025-ps1	UTSW	2	85918255	missense	probably benign	0.28
R2027:Olfr1025-ps1	UTSW	2	85918770	missense	probably damaging	0.99
R2141:Olfr1025-ps1	UTSW	2	85918827	missense	probably null
R4837:Olfr1025-ps1	UTSW	2	85918404	missense	probably benign	0.00
R4945:Olfr1025-ps1	UTSW	2	85918573	missense	possibly damaging	0.81
R5056:Olfr1025-ps1	UTSW	2	85918136	missense	probably damaging	1.00
R5441:Olfr1025-ps1	UTSW	2	85918590	missense	probably benign	0.01
R5960:Olfr1025-ps1	UTSW	2	85918725	missense	probably benign	0.07
R6807:Olfr1025-ps1	UTSW	2	85918038	missense	possibly damaging	0.48
R7320:Olfr1025-ps1	UTSW	2	85918374	missense	probably benign	0.00
R8523:Olfr1025-ps1	UTSW	2	85918063	missense	probably damaging	0.97
R8715:Olfr1025-ps1	UTSW	2	85917929	start codon destroyed	probably null	0.81
R8734:Olfr1025-ps1	UTSW	2	85918649	nonsense	probably null
R9275:Olfr1025-ps1	UTSW	2	85918702	missense	probably benign	0.17
R9427:Olfr1025-ps1	UTSW	2	85918360	missense	probably benign	0.00
R9728:Olfr1025-ps1	UTSW	2	85918543	missense	possibly damaging	0.72
V1662:Olfr1025-ps1	UTSW	2	85918594	missense	probably benign	0.10
Z1176:Olfr1025-ps1	UTSW	2	85918501	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TGGCACAGACCATCCTGATG -3'
(R):5'- TATAGATCAAGGGGTTCAACATCGG -3'

Sequencing Primer
(F):5'- ACAGACCATCCTGATGCTTCG -3'
(R):5'- GTTCAACATCGGGCTTACAAAG -3'

Posted On

2015-04-06