Mutagenetix > Incidental Mutation

Incidental Mutation 'R3871:Tas2r122'

276560

Institutional Source

Beutler Lab

Gene Symbol

Tas2r122

Ensembl Gene

ENSMUSG00000078280

Gene Name

taste receptor, type 2, member 122

Synonyms

Tas2r22, T2R22, mGR22

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.048) question?

Stock #

R3871 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

132710999-132711928 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 132711580 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 117 (S117P)

Ref Sequence

ENSEMBL: ENSMUSP00000100699 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000105077]

AlphaFold

D3YU55

Predicted Effect

probably benign
Transcript: ENSMUST00000105077
AA Change: S117P

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000100699
Gene: ENSMUSG00000078280
AA Change: S117P

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	302	2.3e-73	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aldh1a1	T	A	19: 20,624,753	Y225*	probably null	Het
Bard1	T	C	1: 71,074,940	K294R	probably benign	Het
Bcap29	A	T	12: 31,617,081	S194T	probably benign	Het
Ccdc40	G	A	11: 119,264,281	V1116M	probably damaging	Het
Cntnap5a	A	G	1: 116,060,249	E170G	probably damaging	Het
Crygs	C	T	16: 22,805,551	G102D	possibly damaging	Het
Cyp2c54	T	C	19: 40,072,423	D92G	probably benign	Het
Dpp6	T	C	5: 27,469,465	F197L	probably benign	Het
Filip1l	G	T	16: 57,513,286	K147N	probably damaging	Het
Hrnr	T	A	3: 93,331,874	S3140T	unknown	Het
Igfn1	G	T	1: 135,968,836	H1331N	probably benign	Het
Kalrn	C	T	16: 34,203,856		probably null	Het
Kmt2d	TTGCTGCTGCTGCTGCTGCTGCTGC	TTGCTGCTGCTGCTGCTGC	15: 98,851,021		probably benign	Het
Mfng	A	G	15: 78,756,621	L308P	probably damaging	Het
Nt5e	C	A	9: 88,364,693	N327K	probably benign	Het
Olfr1025-ps1	T	G	2: 85,918,582		probably null	Het
Pgbd1	C	T	13: 21,434,370	R39H	possibly damaging	Het
Phactr4	T	C	4: 132,377,249	T256A	probably benign	Het
Rab24	T	C	13: 55,321,179	D63G	probably damaging	Het
Rubcnl	C	T	14: 75,040,916	P380L	probably benign	Het
Satb2	A	G	1: 56,891,220	S215P	probably damaging	Het
Serpina3b	A	T	12: 104,138,788	I408F	probably damaging	Het
Setx	A	G	2: 29,145,741	D746G	probably damaging	Het
Sf3a2	A	C	10: 80,804,693		probably benign	Het
Snx33	T	C	9: 56,926,740	N15S	probably benign	Het
Snx9	C	T	17: 5,891,781	P61L	probably benign	Het
Sult2a6	T	C	7: 14,254,776	K20E	probably benign	Het
Tmem26	G	A	10: 68,778,732	E326K	probably benign	Het
Tnpo2	T	A	8: 85,054,751	C789S	probably null	Het
Togaram1	A	C	12: 65,002,645	E1285D	probably benign	Het
Ubxn7	T	A	16: 32,381,430	S335T	possibly damaging	Het
Unc119b	G	A	5: 115,130,508	T106M	probably damaging	Het
Usp14	A	G	18: 10,002,370	S314P	possibly damaging	Het
Usp32	T	C	11: 85,081,156	D129G	probably null	Het

Other mutations in Tas2r122

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01712:Tas2r122	APN	6	132711762	missense	possibly damaging	0.87
IGL02478:Tas2r122	APN	6	132711615	missense	possibly damaging	0.81
IGL02605:Tas2r122	APN	6	132711609	missense	probably damaging	0.99
IGL02646:Tas2r122	APN	6	132711790	missense	probably damaging	1.00
IGL02716:Tas2r122	APN	6	132711264	missense	probably damaging	1.00
R0318:Tas2r122	UTSW	6	132711832	missense	possibly damaging	0.90
R0462:Tas2r122	UTSW	6	132711178	missense	probably benign	0.06
R0532:Tas2r122	UTSW	6	132711828	missense	possibly damaging	0.94
R0538:Tas2r122	UTSW	6	132711815	missense	probably benign	0.06
R0570:Tas2r122	UTSW	6	132711811	missense	probably damaging	1.00
R1863:Tas2r122	UTSW	6	132711102	nonsense	probably null
R1966:Tas2r122	UTSW	6	132711194	nonsense	probably null
R2001:Tas2r122	UTSW	6	132711622	missense	possibly damaging	0.91
R3500:Tas2r122	UTSW	6	132711560	missense	probably damaging	1.00
R4174:Tas2r122	UTSW	6	132711876	missense	probably damaging	0.96
R5533:Tas2r122	UTSW	6	132711430	missense	probably damaging	1.00
R5567:Tas2r122	UTSW	6	132711372	missense	probably benign	0.28
R6455:Tas2r122	UTSW	6	132711663	nonsense	probably null
R6716:Tas2r122	UTSW	6	132711897	missense	probably damaging	0.98
R8812:Tas2r122	UTSW	6	132711739	missense	probably benign	0.09
R9139:Tas2r122	UTSW	6	132711816	missense	probably benign	0.33
R9720:Tas2r122	UTSW	6	132711671	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- AGGCTATGGTAGACTAAGAAACCAC -3'
(R):5'- TTGGTTGGCCATCTCCAGAATG -3'

Sequencing Primer
(F):5'- ACCACTTAGAGTATCTAAAACAGGG -3'
(R):5'- CCAGAATGTGTGTTCTATGGACAAC -3'

Posted On

2015-04-06