Mutagenetix > Incidental Mutation

Incidental Mutation 'R3873:Prl7d1'

276687

Institutional Source

Beutler Lab

Gene Symbol

Prl7d1

Ensembl Gene

ENSMUSG00000021348

Gene Name

prolactin family 7, subfamily d, member 1

Synonyms

Plfr, PLF-RP, PRP

MMRRC Submission

040791-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3873 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

27892981-27900720 bp(-) (GRCm39)

Type of Mutation

start codon destroyed

DNA Base Change (assembly)

A to G at 27900651 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Threonine at position 1 (M1T)

Ref Sequence

ENSEMBL: ENSMUSP00000021776 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000021776] [ENSMUST00000224026]

AlphaFold

P04769

Predicted Effect

probably null
Transcript: ENSMUST00000021776
AA Change: M1T

PolyPhen 2 Score 0.915 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000021776
Gene: ENSMUSG00000021348
AA Change: M1T

Domain	Start	End	E-Value	Type
Pfam:Hormone_1	17	240	1.1e-78	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145879

Predicted Effect

probably null
Transcript: ENSMUST00000224026
AA Change: M1T

PolyPhen 2 Score 0.533 (Sensitivity: 0.88; Specificity: 0.90)

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.6%
20x: 95.9%

Validation Efficiency

100% (50/50)

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca5	T	A	11: 110,201,059 (GRCm39)	Y447F	probably damaging	Het
Acaca	A	G	11: 84,203,547 (GRCm39)		probably benign	Het
Adam5	T	C	8: 25,305,125 (GRCm39)	T110A	probably benign	Het
Agtpbp1	T	C	13: 59,608,410 (GRCm39)	M175V	possibly damaging	Het
Akt1	T	C	12: 112,622,967 (GRCm39)	N367S	probably benign	Het
Ankrd61	T	A	5: 143,828,646 (GRCm39)	T67S	probably damaging	Het
Arl8a	T	C	1: 135,080,610 (GRCm39)		probably null	Het
Arvcf	G	A	16: 18,221,783 (GRCm39)	R736Q	probably damaging	Het
Baz2a	T	A	10: 127,959,979 (GRCm39)	M1419K	probably damaging	Het
Cep295	A	T	9: 15,244,661 (GRCm39)	V1265E	probably damaging	Het
Cfap299	A	T	5: 98,885,482 (GRCm39)	I130F	probably damaging	Het
Cyp2c29	A	G	19: 39,317,588 (GRCm39)	D397G	probably damaging	Het
Dlgap4	T	C	2: 156,591,267 (GRCm39)	S818P	probably benign	Het
Dnah2	A	G	11: 69,320,174 (GRCm39)	I3965T	probably damaging	Het
Dst	A	G	1: 34,328,701 (GRCm39)	T4590A	probably damaging	Het
Eif1ad10	T	C	12: 88,216,476 (GRCm39)	D132G	unknown	Het
Fscb	G	T	12: 64,519,906 (GRCm39)	P520Q	unknown	Het
Gli1	T	A	10: 127,167,225 (GRCm39)	N676I	probably damaging	Het
Hspg2	T	C	4: 137,266,660 (GRCm39)	I1916T	probably damaging	Het
Igfals	G	A	17: 25,100,579 (GRCm39)	V557I	possibly damaging	Het
Il7	A	T	3: 7,669,224 (GRCm39)	V4D	probably damaging	Het
Itgae	T	A	11: 73,004,442 (GRCm39)	I243N	probably damaging	Het
Itpa	T	G	2: 130,522,930 (GRCm39)	S176A	probably damaging	Het
Klhl13	T	C	X: 23,151,415 (GRCm39)	D21G	probably benign	Het
Krt26	T	C	11: 99,225,570 (GRCm39)	K304E	probably damaging	Het
Ly6g6e	T	C	17: 35,296,159 (GRCm39)	V10A	probably benign	Het
Morc3	G	A	16: 93,659,324 (GRCm39)	V411I	probably damaging	Het
Mrpl32	G	T	13: 14,787,630 (GRCm39)		probably benign	Het
Ncald	G	A	15: 37,397,497 (GRCm39)	A61V	probably damaging	Het
Nek2	T	C	1: 191,559,320 (GRCm39)	V275A	probably benign	Het
Or12d12	T	A	17: 37,610,870 (GRCm39)	T148S	probably benign	Het
Or4f6	T	C	2: 111,838,668 (GRCm39)	T288A	possibly damaging	Het
Or5l13	T	A	2: 87,779,874 (GRCm39)	R234S	probably damaging	Het
Or5m10b	T	A	2: 85,699,306 (GRCm39)	Y123*	probably null	Het
Pgam5	T	C	5: 110,413,465 (GRCm39)	Y210C	probably damaging	Het
Phf5a	T	C	15: 81,754,628 (GRCm39)	N50D	probably benign	Het
Sacs	A	G	14: 61,429,735 (GRCm39)	K595R	possibly damaging	Het
Scyl3	A	G	1: 163,778,206 (GRCm39)	N448S	probably benign	Het
Serpinb9g	A	T	13: 33,670,518 (GRCm39)	D2V	probably benign	Het
Sgsh	A	G	11: 119,241,773 (GRCm39)	L111P	probably damaging	Het
Smg1	T	C	7: 117,753,885 (GRCm39)		probably benign	Het
Taar1	T	C	10: 23,796,482 (GRCm39)	L60P	probably damaging	Het
Tmem51	T	C	4: 141,759,059 (GRCm39)	T230A	probably damaging	Het
Ubr4	T	C	4: 139,151,301 (GRCm39)	V623A	probably damaging	Het
Usp34	C	T	11: 23,439,033 (GRCm39)	P3532S	possibly damaging	Het
Vipr2	T	C	12: 116,099,724 (GRCm39)		probably benign	Het
Vmn2r16	T	C	5: 109,488,177 (GRCm39)	M350T	probably benign	Het
Vmn2r22	T	C	6: 123,614,339 (GRCm39)	E417G	possibly damaging	Het
Vmn2r9	C	A	5: 108,995,701 (GRCm39)	V316F	probably benign	Het
Zfp53	C	T	17: 21,728,893 (GRCm39)	P309S	probably damaging	Het

Other mutations in Prl7d1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01390:Prl7d1	APN	13	27,894,149 (GRCm39)	missense	possibly damaging	0.91
IGL01735:Prl7d1	APN	13	27,898,372 (GRCm39)	missense	possibly damaging	0.83
IGL02139:Prl7d1	APN	13	27,896,066 (GRCm39)	missense	probably damaging	1.00
PIT4305001:Prl7d1	UTSW	13	27,898,320 (GRCm39)	missense	possibly damaging	0.90
PIT4531001:Prl7d1	UTSW	13	27,894,161 (GRCm39)	missense	probably damaging	1.00
R0555:Prl7d1	UTSW	13	27,896,038 (GRCm39)	missense	probably benign	0.13
R0625:Prl7d1	UTSW	13	27,894,123 (GRCm39)	missense	probably benign	0.10
R0631:Prl7d1	UTSW	13	27,894,165 (GRCm39)	missense	probably benign	0.32
R0837:Prl7d1	UTSW	13	27,898,321 (GRCm39)	missense	probably benign	0.06
R1403:Prl7d1	UTSW	13	27,893,180 (GRCm39)	missense	possibly damaging	0.89
R1403:Prl7d1	UTSW	13	27,893,180 (GRCm39)	missense	possibly damaging	0.89
R1459:Prl7d1	UTSW	13	27,893,240 (GRCm39)	missense	possibly damaging	0.81
R1643:Prl7d1	UTSW	13	27,896,114 (GRCm39)	missense	possibly damaging	0.53
R1691:Prl7d1	UTSW	13	27,893,365 (GRCm39)	missense	probably damaging	0.97
R2016:Prl7d1	UTSW	13	27,894,156 (GRCm39)	missense	probably damaging	1.00
R3874:Prl7d1	UTSW	13	27,900,651 (GRCm39)	start codon destroyed	probably null	0.92
R5251:Prl7d1	UTSW	13	27,893,227 (GRCm39)	missense	probably benign	0.33
R5275:Prl7d1	UTSW	13	27,893,230 (GRCm39)	missense	probably damaging	0.99
R5295:Prl7d1	UTSW	13	27,893,230 (GRCm39)	missense	probably damaging	0.99
R5398:Prl7d1	UTSW	13	27,894,057 (GRCm39)	missense	probably damaging	1.00
R6798:Prl7d1	UTSW	13	27,893,380 (GRCm39)	critical splice acceptor site	probably null
R6818:Prl7d1	UTSW	13	27,898,454 (GRCm39)	missense	probably benign	0.07
R6825:Prl7d1	UTSW	13	27,894,125 (GRCm39)	missense	probably benign	0.01
R7478:Prl7d1	UTSW	13	27,894,168 (GRCm39)	nonsense	probably null
R7795:Prl7d1	UTSW	13	27,893,263 (GRCm39)	missense	probably damaging	1.00
R7995:Prl7d1	UTSW	13	27,894,054 (GRCm39)	missense	probably benign
R8193:Prl7d1	UTSW	13	27,893,230 (GRCm39)	missense
R9313:Prl7d1	UTSW	13	27,893,182 (GRCm39)	missense	probably benign
R9424:Prl7d1	UTSW	13	27,894,185 (GRCm39)	missense	probably benign	0.00
R9430:Prl7d1	UTSW	13	27,898,360 (GRCm39)	missense	possibly damaging	0.80
R9733:Prl7d1	UTSW	13	27,898,339 (GRCm39)	missense	probably benign	0.00
R9758:Prl7d1	UTSW	13	27,893,260 (GRCm39)	missense	possibly damaging	0.91

Predicted Primers

PCR Primer
(F):5'- GAAAGGTTTTCTCAGATCCACTGG -3'
(R):5'- GTTCATGGTATCTAGGCTACTTAACC -3'

Sequencing Primer
(F):5'- TCAGATCCACTGGGCTCTAC -3'
(R):5'- GGTATCTAGGCTACTTAACCCTGAG -3'

Posted On

2015-04-06