Mutagenetix > Incidental Mutation

Incidental Mutation 'R3874:Tnc'

276711

Institutional Source

Beutler Lab

Gene Symbol

Tnc

Ensembl Gene

ENSMUSG00000028364

Gene Name

tenascin C

Synonyms

TN, TN-C, hexabrachion, tenascin-C, C130033P17Rik, cytotactin, Hxb

MMRRC Submission

040792-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3874 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

63959785-64047015 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 64008710 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 860 (I860F)

Ref Sequence

ENSEMBL: ENSMUSP00000103000 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030056] [ENSMUST00000107371] [ENSMUST00000107372] [ENSMUST00000107377]

AlphaFold

Q80YX1

Predicted Effect

probably damaging
Transcript: ENSMUST00000030056
AA Change: I860F

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000030056
Gene: ENSMUSG00000028364
AA Change: I860F

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	3.4e-4	SMART
FN3	1528	1606	1.55e-7	SMART
FN3	1617	1694	1.53e-6	SMART
FN3	1705	1782	7.75e-8	SMART
FBG	1797	2007	4.08e-124	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000107371

SMART Domains

Protein: ENSMUSP00000102994
Gene: ENSMUSG00000028364

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
Pfam:hEGF	173	185	4e-4	PFAM
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000107372
AA Change: I860F

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000102995
Gene: ENSMUSG00000028364
AA Change: I860F

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	2.75e0	SMART
FN3	1529	1608	3.4e-4	SMART
FN3	1619	1697	1.55e-7	SMART
FN3	1708	1785	1.53e-6	SMART
FN3	1796	1873	7.75e-8	SMART
FBG	1888	2098	4.08e-124	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000107377
AA Change: I860F

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000103000
Gene: ENSMUSG00000028364
AA Change: I860F

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	3.4e-4	SMART
FN3	1528	1606	1.55e-7	SMART
FN3	1617	1694	1.53e-6	SMART
FN3	1705	1782	7.75e-8	SMART
FBG	1797	2007	4.08e-124	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000135042

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141428

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.5%
20x: 95.9%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration. [provided by RefSeq, Jul 2011]
PHENOTYPE: Mice homozygous for several different targeted mutations show variable behavioral and nervous system phenotypes such as abnormal circadian rhythm, anxiety behavior, novelty-induced activity, swimming, impaired synaptic plasticity, long term potentiation and serotonin and dopamine neurotransmission. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9130213A22Rik	C	A	11: 69,121,475 (GRCm38)	G26*	probably null	Het
Abca5	T	A	11: 110,310,233 (GRCm38)	Y447F	probably damaging	Het
Acox2	A	G	14: 8,248,061 (GRCm38)	I407T	probably benign	Het
Adam8	T	C	7: 139,987,607 (GRCm38)	N408D	probably damaging	Het
Adgre1	A	T	17: 57,401,925 (GRCm38)	T39S	probably benign	Het
Akap12	G	A	10: 4,357,590 (GRCm38)	V1467I	probably benign	Het
Arid1b	T	A	17: 5,336,515 (GRCm38)		probably null	Het
Atp2c2	A	G	8: 119,735,296 (GRCm38)	I303V	possibly damaging	Het
Bpifc	C	T	10: 85,991,254 (GRCm38)	V144I	probably benign	Het
Camk4	A	G	18: 33,158,854 (GRCm38)	E189G	possibly damaging	Het
Casz1	A	G	4: 148,939,589 (GRCm38)		probably benign	Het
Ccdc134	T	C	15: 82,131,442 (GRCm38)	V41A	possibly damaging	Het
Chrd	A	T	16: 20,738,910 (GRCm38)	T753S	probably damaging	Het
Cracd	A	G	5: 76,840,892 (GRCm38)	D30G	probably damaging	Het
Cyb561a3	T	C	19: 10,585,371 (GRCm38)	V125A	probably benign	Het
D630039A03Rik	T	A	4: 57,910,606 (GRCm38)	T69S	probably benign	Het
Dchs1	A	G	7: 105,761,635 (GRCm38)	F1687S	probably damaging	Het
Dlgap4	T	C	2: 156,749,347 (GRCm38)	S818P	probably benign	Het
Dnah2	A	G	11: 69,429,348 (GRCm38)	I3965T	probably damaging	Het
Dnai2	G	T	11: 114,732,955 (GRCm38)	G15W	probably damaging	Het
Dsg1c	A	G	18: 20,277,052 (GRCm38)	I526V	probably benign	Het
Far2	G	A	6: 148,150,591 (GRCm38)	E123K	probably benign	Het
Gli1	A	T	10: 127,330,219 (GRCm38)	V1055E	probably damaging	Het
Hand2	G	T	8: 57,321,976 (GRCm38)	A24S	probably benign	Het
Helb	T	C	10: 120,106,037 (GRCm38)	I249V	probably benign	Het
Hspa4l	G	A	3: 40,772,642 (GRCm38)	V492M	probably damaging	Het
Hspg2	T	C	4: 137,539,349 (GRCm38)	I1916T	probably damaging	Het
Igfals	G	A	17: 24,881,605 (GRCm38)	V557I	possibly damaging	Het
Itpa	T	G	2: 130,681,010 (GRCm38)	S176A	probably damaging	Het
Kcnu1	T	A	8: 25,885,317 (GRCm38)	L353H	probably damaging	Het
Klhl1	A	G	14: 96,518,179 (GRCm38)	F47L	probably benign	Het
Klhl13	T	C	X: 23,285,176 (GRCm38)	D21G	probably benign	Het
Krt26	T	C	11: 99,334,744 (GRCm38)	K304E	probably damaging	Het
Krt9	C	T	11: 100,190,849 (GRCm38)	V285I	probably damaging	Het
Mansc1	T	C	6: 134,610,183 (GRCm38)	R344G	possibly damaging	Het
Mier2	G	T	10: 79,541,797 (GRCm38)	P439T	possibly damaging	Het
Mppe1	T	A	18: 67,225,886 (GRCm38)		probably null	Het
Nedd4l	G	A	18: 65,167,535 (GRCm38)	A243T	probably benign	Het
Notch4	T	A	17: 34,578,069 (GRCm38)	C934*	probably null	Het
Nsmce3	C	T	7: 64,872,168 (GRCm38)	D251N	probably damaging	Het
Or10a49	C	T	7: 108,869,128 (GRCm38)	V9M	probably damaging	Het
Or12d12	T	A	17: 37,299,979 (GRCm38)	T148S	probably benign	Het
Or3a10	A	G	11: 74,044,699 (GRCm38)	C192R	probably damaging	Het
Or4f6	T	C	2: 112,008,323 (GRCm38)	T288A	possibly damaging	Het
Or5l13	T	A	2: 87,949,530 (GRCm38)	R234S	probably damaging	Het
Or8g23	A	G	9: 39,060,174 (GRCm38)	I164T	probably benign	Het
Pdzd7	T	C	19: 45,045,628 (GRCm38)	T6A	probably benign	Het
Picalm	T	A	7: 90,189,219 (GRCm38)	F493Y	probably damaging	Het
Prl7d1	A	G	13: 27,716,668 (GRCm38)	M1T	probably null	Het
Prl8a1	T	C	13: 27,575,458 (GRCm38)	K199E	possibly damaging	Het
Rims1	C	T	1: 22,428,489 (GRCm38)	R764H	probably damaging	Het
Rnf17	G	T	14: 56,475,413 (GRCm38)	R779L	possibly damaging	Het
Rufy2	T	C	10: 62,998,137 (GRCm38)	L294P	probably damaging	Het
Sgsh	A	G	11: 119,350,947 (GRCm38)	L111P	probably damaging	Het
Slc22a30	G	T	19: 8,336,849 (GRCm38)	T491K	probably benign	Het
Slc35b3	T	C	13: 38,943,068 (GRCm38)	N20D	possibly damaging	Het
Slc5a4a	T	C	10: 76,181,655 (GRCm38)	F429L	probably benign	Het
Sulf1	T	C	1: 12,817,412 (GRCm38)	I270T	probably damaging	Het
Tmem51	T	C	4: 142,031,748 (GRCm38)	T230A	probably damaging	Het
Trh	A	T	6: 92,243,698 (GRCm38)	V61E	possibly damaging	Het
Ttn	T	G	2: 76,754,099 (GRCm38)	T22222P	probably damaging	Het
Uroc1	A	T	6: 90,361,512 (GRCm38)	K652*	probably null	Het
Usp34	C	T	11: 23,489,033 (GRCm38)	P3532S	possibly damaging	Het
Vmn2r120	A	T	17: 57,524,954 (GRCm38)	F278L	probably benign	Het
Vmn2r7	A	G	3: 64,719,611 (GRCm38)	F86L	possibly damaging	Het
Vmn2r87	A	T	10: 130,479,987 (GRCm38)	I70K	possibly damaging	Het
Vps13a	C	T	19: 16,744,953 (GRCm38)	A332T	probably benign	Het
Zfp568	T	A	7: 30,023,396 (GRCm38)	C589S	probably damaging	Het

Other mutations in Tnc

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00229:Tnc	APN	4	64,016,824 (GRCm38)	splice site	probably benign
IGL00531:Tnc	APN	4	63,971,153 (GRCm38)	splice site	probably benign
IGL00674:Tnc	APN	4	63,965,607 (GRCm38)	missense	probably damaging	1.00
IGL01015:Tnc	APN	4	64,017,334 (GRCm38)	missense	probably benign	0.19
IGL01090:Tnc	APN	4	64,000,080 (GRCm38)	missense	probably damaging	1.00
IGL01310:Tnc	APN	4	64,013,077 (GRCm38)	missense	probably benign	0.03
IGL01331:Tnc	APN	4	63,982,875 (GRCm38)	missense	probably damaging	0.99
IGL01393:Tnc	APN	4	64,014,054 (GRCm38)	splice site	probably benign
IGL01411:Tnc	APN	4	64,000,722 (GRCm38)	missense	probably damaging	0.96
IGL01472:Tnc	APN	4	64,006,419 (GRCm38)	missense	probably benign	0.00
IGL01552:Tnc	APN	4	63,970,408 (GRCm38)	missense	probably damaging	1.00
IGL01661:Tnc	APN	4	63,970,307 (GRCm38)	splice site	probably benign
IGL01669:Tnc	APN	4	64,000,701 (GRCm38)	missense	probably damaging	1.00
IGL01912:Tnc	APN	4	64,008,740 (GRCm38)	missense	probably damaging	1.00
IGL02028:Tnc	APN	4	63,966,672 (GRCm38)	splice site	probably benign
IGL02100:Tnc	APN	4	64,000,161 (GRCm38)	missense	possibly damaging	0.84
IGL02549:Tnc	APN	4	64,015,072 (GRCm38)	missense	probably damaging	1.00
IGL02642:Tnc	APN	4	63,965,579 (GRCm38)	splice site	probably benign
IGL02712:Tnc	APN	4	63,975,256 (GRCm38)	missense	probably damaging	1.00
IGL02876:Tnc	APN	4	64,015,101 (GRCm38)	missense	possibly damaging	0.56
IGL02886:Tnc	APN	4	64,000,107 (GRCm38)	missense	probably damaging	0.96
IGL02972:Tnc	APN	4	63,976,478 (GRCm38)	missense	probably benign	0.11
IGL03073:Tnc	APN	4	63,971,224 (GRCm38)	missense	possibly damaging	0.58
IGL03116:Tnc	APN	4	64,014,033 (GRCm38)	missense	probably damaging	1.00
IGL03181:Tnc	APN	4	63,967,306 (GRCm38)	missense	possibly damaging	0.95
IGL03358:Tnc	APN	4	64,017,615 (GRCm38)	nonsense	probably null
tancredo	UTSW	4	63,993,297 (GRCm38)	nonsense	probably null
BB009:Tnc	UTSW	4	64,008,620 (GRCm38)	missense	probably benign
BB019:Tnc	UTSW	4	64,008,620 (GRCm38)	missense	probably benign
P0020:Tnc	UTSW	4	64,008,857 (GRCm38)	missense	possibly damaging	0.63
PIT4377001:Tnc	UTSW	4	64,017,736 (GRCm38)	missense	probably damaging	1.00
PIT4403001:Tnc	UTSW	4	63,964,667 (GRCm38)	missense	probably damaging	1.00
PIT4468001:Tnc	UTSW	4	63,964,667 (GRCm38)	missense	probably damaging	1.00
R0243:Tnc	UTSW	4	63,970,420 (GRCm38)	missense	probably damaging	0.98
R0362:Tnc	UTSW	4	64,017,442 (GRCm38)	missense	probably damaging	1.00
R0410:Tnc	UTSW	4	64,007,694 (GRCm38)	missense	probably benign	0.00
R0420:Tnc	UTSW	4	64,000,159 (GRCm38)	missense	probably benign	0.00
R0540:Tnc	UTSW	4	64,020,455 (GRCm38)	missense	probably damaging	1.00
R0650:Tnc	UTSW	4	64,008,734 (GRCm38)	missense	probably benign	0.00
R1019:Tnc	UTSW	4	63,962,082 (GRCm38)	missense	probably damaging	1.00
R1102:Tnc	UTSW	4	64,020,468 (GRCm38)	missense	probably benign	0.05
R1126:Tnc	UTSW	4	64,018,120 (GRCm38)	missense	probably damaging	0.99
R1141:Tnc	UTSW	4	64,013,994 (GRCm38)	missense	probably damaging	1.00
R1142:Tnc	UTSW	4	64,013,994 (GRCm38)	missense	probably damaging	1.00
R1307:Tnc	UTSW	4	64,008,859 (GRCm38)	missense	probably damaging	0.98
R1322:Tnc	UTSW	4	64,013,994 (GRCm38)	missense	probably damaging	1.00
R1414:Tnc	UTSW	4	63,965,695 (GRCm38)	splice site	probably benign
R1470:Tnc	UTSW	4	63,966,574 (GRCm38)	missense	probably damaging	1.00
R1470:Tnc	UTSW	4	63,966,574 (GRCm38)	missense	probably damaging	1.00
R1499:Tnc	UTSW	4	63,964,754 (GRCm38)	missense	probably benign	0.15
R1506:Tnc	UTSW	4	64,007,684 (GRCm38)	missense	possibly damaging	0.90
R1597:Tnc	UTSW	4	64,006,384 (GRCm38)	missense	probably benign
R1750:Tnc	UTSW	4	63,972,735 (GRCm38)	missense	probably damaging	1.00
R1765:Tnc	UTSW	4	64,013,994 (GRCm38)	missense	probably damaging	1.00
R1783:Tnc	UTSW	4	64,018,096 (GRCm38)	missense	probably damaging	0.98
R1808:Tnc	UTSW	4	63,999,931 (GRCm38)	missense	probably damaging	1.00
R1903:Tnc	UTSW	4	64,000,062 (GRCm38)	missense	probably benign	0.00
R1932:Tnc	UTSW	4	63,993,025 (GRCm38)	critical splice donor site	probably null
R1941:Tnc	UTSW	4	64,014,964 (GRCm38)	missense	probably damaging	1.00
R1983:Tnc	UTSW	4	63,984,630 (GRCm38)	missense	possibly damaging	0.95
R2024:Tnc	UTSW	4	63,964,621 (GRCm38)	missense	probably damaging	1.00
R2075:Tnc	UTSW	4	63,995,666 (GRCm38)	missense	possibly damaging	0.94
R2327:Tnc	UTSW	4	63,975,238 (GRCm38)	missense	possibly damaging	0.78
R2444:Tnc	UTSW	4	64,014,963 (GRCm38)	missense	probably damaging	1.00
R2982:Tnc	UTSW	4	64,020,519 (GRCm38)	missense	possibly damaging	0.81
R4110:Tnc	UTSW	4	64,014,951 (GRCm38)	missense	probably damaging	1.00
R4360:Tnc	UTSW	4	64,016,924 (GRCm38)	missense	probably benign	0.35
R4371:Tnc	UTSW	4	63,970,351 (GRCm38)	missense	probably damaging	1.00
R4434:Tnc	UTSW	4	64,007,829 (GRCm38)	missense	possibly damaging	0.91
R4438:Tnc	UTSW	4	64,007,829 (GRCm38)	missense	possibly damaging	0.91
R4570:Tnc	UTSW	4	63,995,672 (GRCm38)	missense	probably damaging	0.99
R4595:Tnc	UTSW	4	63,995,745 (GRCm38)	missense	probably damaging	1.00
R4749:Tnc	UTSW	4	63,995,639 (GRCm38)	missense	possibly damaging	0.56
R4756:Tnc	UTSW	4	63,967,343 (GRCm38)	missense	probably damaging	0.99
R4824:Tnc	UTSW	4	64,017,620 (GRCm38)	nonsense	probably null
R4957:Tnc	UTSW	4	63,976,556 (GRCm38)	missense	probably damaging	1.00
R4977:Tnc	UTSW	4	64,006,248 (GRCm38)	missense	possibly damaging	0.82
R5001:Tnc	UTSW	4	64,000,062 (GRCm38)	missense	probably benign	0.16
R5001:Tnc	UTSW	4	63,984,489 (GRCm38)	missense	probably damaging	1.00
R5015:Tnc	UTSW	4	64,006,502 (GRCm38)	missense	probably damaging	1.00
R5049:Tnc	UTSW	4	64,017,986 (GRCm38)	missense	probably damaging	1.00
R5066:Tnc	UTSW	4	63,975,229 (GRCm38)	missense	probably damaging	0.96
R5073:Tnc	UTSW	4	64,020,411 (GRCm38)	missense	probably damaging	1.00
R5116:Tnc	UTSW	4	63,967,215 (GRCm38)	critical splice donor site	probably null
R5195:Tnc	UTSW	4	63,967,252 (GRCm38)	missense	probably damaging	1.00
R5200:Tnc	UTSW	4	63,971,278 (GRCm38)	missense	probably damaging	1.00
R5221:Tnc	UTSW	4	63,993,297 (GRCm38)	nonsense	probably null
R5237:Tnc	UTSW	4	63,962,096 (GRCm38)	missense	probably damaging	1.00
R5265:Tnc	UTSW	4	63,993,206 (GRCm38)	missense	probably benign	0.00
R5275:Tnc	UTSW	4	63,964,730 (GRCm38)	nonsense	probably null
R5346:Tnc	UTSW	4	64,008,655 (GRCm38)	missense	probably benign
R5409:Tnc	UTSW	4	64,007,417 (GRCm38)	missense	probably damaging	1.00
R5409:Tnc	UTSW	4	63,966,536 (GRCm38)	missense	probably damaging	1.00
R5469:Tnc	UTSW	4	64,013,925 (GRCm38)	splice site	probably null
R5518:Tnc	UTSW	4	64,017,679 (GRCm38)	missense	probably damaging	1.00
R5560:Tnc	UTSW	4	64,008,709 (GRCm38)	missense	probably damaging	1.00
R5588:Tnc	UTSW	4	64,006,422 (GRCm38)	missense	possibly damaging	0.57
R5686:Tnc	UTSW	4	64,007,730 (GRCm38)	splice site	probably null
R5686:Tnc	UTSW	4	64,008,795 (GRCm38)	missense	possibly damaging	0.78
R5837:Tnc	UTSW	4	64,013,214 (GRCm38)	missense	probably damaging	1.00
R5976:Tnc	UTSW	4	64,018,166 (GRCm38)	missense	probably benign	0.17
R6156:Tnc	UTSW	4	63,970,352 (GRCm38)	missense	probably damaging	1.00
R6182:Tnc	UTSW	4	64,008,796 (GRCm38)	missense	probably damaging	0.99
R6360:Tnc	UTSW	4	64,000,733 (GRCm38)	missense	probably damaging	1.00
R6416:Tnc	UTSW	4	64,007,816 (GRCm38)	missense	probably benign	0.05
R6778:Tnc	UTSW	4	63,995,598 (GRCm38)	missense	probably benign	0.12
R6798:Tnc	UTSW	4	63,965,604 (GRCm38)	missense	probably benign	0.02
R6799:Tnc	UTSW	4	63,965,604 (GRCm38)	missense	probably benign	0.02
R6943:Tnc	UTSW	4	63,982,745 (GRCm38)	missense	probably damaging	0.97
R7027:Tnc	UTSW	4	63,984,589 (GRCm38)	missense	probably benign	0.02
R7183:Tnc	UTSW	4	64,013,128 (GRCm38)	missense	probably damaging	1.00
R7204:Tnc	UTSW	4	63,971,155 (GRCm38)	splice site	probably null
R7317:Tnc	UTSW	4	63,972,722 (GRCm38)	missense	probably damaging	0.99
R7323:Tnc	UTSW	4	63,971,232 (GRCm38)	missense	probably damaging	0.96
R7327:Tnc	UTSW	4	63,964,762 (GRCm38)	splice site	probably null
R7382:Tnc	UTSW	4	64,014,043 (GRCm38)	nonsense	probably null
R7399:Tnc	UTSW	4	64,020,657 (GRCm38)	start gained	probably benign
R7479:Tnc	UTSW	4	64,017,628 (GRCm38)	missense	possibly damaging	0.95
R7585:Tnc	UTSW	4	64,020,411 (GRCm38)	missense	probably damaging	1.00
R7932:Tnc	UTSW	4	64,008,620 (GRCm38)	missense	probably benign
R7947:Tnc	UTSW	4	64,017,343 (GRCm38)	missense	probably damaging	1.00
R7974:Tnc	UTSW	4	64,000,724 (GRCm38)	missense	possibly damaging	0.84
R7991:Tnc	UTSW	4	64,008,746 (GRCm38)	missense	probably benign	0.42
R8004:Tnc	UTSW	4	63,984,657 (GRCm38)	missense	probably benign	0.04
R8080:Tnc	UTSW	4	63,976,469 (GRCm38)	missense	possibly damaging	0.52
R8109:Tnc	UTSW	4	64,008,763 (GRCm38)	missense	probably benign	0.11
R8145:Tnc	UTSW	4	64,017,479 (GRCm38)	missense	probably benign
R8340:Tnc	UTSW	4	64,007,799 (GRCm38)	missense	probably damaging	1.00
R8360:Tnc	UTSW	4	63,967,274 (GRCm38)	missense	probably benign	0.00
R8671:Tnc	UTSW	4	64,017,446 (GRCm38)	missense	probably damaging	1.00
R8691:Tnc	UTSW	4	63,962,076 (GRCm38)	missense	probably damaging	1.00
R8759:Tnc	UTSW	4	64,006,264 (GRCm38)	missense	possibly damaging	0.86
R8864:Tnc	UTSW	4	63,993,059 (GRCm38)	missense	probably damaging	0.98
R8927:Tnc	UTSW	4	64,007,358 (GRCm38)	missense	probably damaging	1.00
R8928:Tnc	UTSW	4	64,007,358 (GRCm38)	missense	probably damaging	1.00
R8949:Tnc	UTSW	4	64,008,850 (GRCm38)	missense	probably damaging	1.00
R8956:Tnc	UTSW	4	64,000,733 (GRCm38)	missense	probably damaging	1.00
R9016:Tnc	UTSW	4	64,017,094 (GRCm38)	missense	probably benign	0.23
R9049:Tnc	UTSW	4	64,000,010 (GRCm38)	missense	possibly damaging	0.83
R9097:Tnc	UTSW	4	63,970,385 (GRCm38)	missense	possibly damaging	0.62
R9114:Tnc	UTSW	4	63,972,736 (GRCm38)	missense	probably benign	0.03
R9151:Tnc	UTSW	4	64,020,449 (GRCm38)	missense	possibly damaging	0.46
R9488:Tnc	UTSW	4	63,995,705 (GRCm38)	missense	probably damaging	0.99
R9537:Tnc	UTSW	4	63,966,584 (GRCm38)	missense	probably damaging	0.99
R9666:Tnc	UTSW	4	64,007,808 (GRCm38)	missense	probably damaging	1.00
R9700:Tnc	UTSW	4	64,014,949 (GRCm38)	missense	probably damaging	0.99
R9703:Tnc	UTSW	4	63,971,175 (GRCm38)	missense	probably benign	0.00
R9771:Tnc	UTSW	4	64,007,363 (GRCm38)	missense	probably damaging	1.00
S24628:Tnc	UTSW	4	64,018,012 (GRCm38)	missense	probably damaging	1.00
Z1177:Tnc	UTSW	4	64,007,426 (GRCm38)	nonsense	probably null
Z1177:Tnc	UTSW	4	63,960,544 (GRCm38)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- CCATCTTTAGTGCTATATGGGCC -3'
(R):5'- TGCTGCAAAGTCCCCACTAC -3'

Sequencing Primer
(F):5'- CTATATGGGCCTGGAGGACTC -3'
(R):5'- GCCGGCATTGACCCTTTG -3'

Posted On

2015-04-06