Mutagenetix > Incidental Mutation

Incidental Mutation 'R3876:Oprk1'

276823

Institutional Source

Beutler Lab

Gene Symbol

Oprk1

Ensembl Gene

ENSMUSG00000025905

Gene Name

opioid receptor, kappa 1

Synonyms

Oprk2, R21, KOR-1

MMRRC Submission

041606-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.266) question?

Stock #

R3876 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

5658689-5676354 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 5672884 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 340 (C340*)

Ref Sequence

ENSEMBL: ENSMUSP00000125105 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000027038] [ENSMUST00000160339] [ENSMUST00000160777]

AlphaFold

P33534

Predicted Effect

probably null
Transcript: ENSMUST00000027038
AA Change: C340*

SMART Domains

Protein: ENSMUSP00000027038
Gene: ENSMUSG00000025905
AA Change: C340*

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	67	280	4.8e-8	PFAM
Pfam:7TM_GPCR_Srsx	70	345	5.4e-14	PFAM
Pfam:7tm_1	76	330	2.6e-67	PFAM
Pfam:7TM_GPCR_Srv	79	345	6.8e-9	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000159083

Predicted Effect

probably null
Transcript: ENSMUST00000160339
AA Change: C340*

SMART Domains

Protein: ENSMUSP00000124030
Gene: ENSMUSG00000025905
AA Change: C340*

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	67	284	1.1e-7	PFAM
Pfam:7TM_GPCR_Srsx	70	345	5.4e-14	PFAM
Pfam:7tm_1	76	330	2.6e-60	PFAM
Pfam:7TM_GPCR_Srv	78	345	5.2e-9	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000160777
AA Change: C340*

SMART Domains

Protein: ENSMUSP00000125105
Gene: ENSMUSG00000025905
AA Change: C340*

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	67	280	4.8e-8	PFAM
Pfam:7TM_GPCR_Srsx	70	345	5.4e-14	PFAM
Pfam:7tm_1	76	330	2.6e-67	PFAM
Pfam:7TM_GPCR_Srv	79	345	6.8e-9	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.5%
20x: 95.9%

Validation Efficiency

100% (53/53)

MGI Phenotype

FUNCTION: This gene encodes an opioid receptor, which is a member of the 7 transmembrane-spanning G protein-coupled receptor family. It functions as a receptor for endogenous ligands, as well as a receptor for various synthetic opioids. Ligand binding results in inhibition of adenylate cyclase activity and neurotransmitter release. This opioid receptor plays a role in the perception of pain and mediating the hypolocomotor, analgesic and aversive actions of synthetic opioids. Variations in this gene have also been associated with alcohol dependence and opiate addiction. Alternatively spliced transcript variants have been found for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Jan 2016]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit impaired response to morphine and an opioid agonist, abnormal pain threshold, and increased litter size. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4931414P19Rik	G	A	14: 54,828,857 (GRCm39)	R215*	probably null	Het
Brinp2	C	A	1: 158,074,416 (GRCm39)	L568F	probably damaging	Het
Brip1	T	C	11: 86,043,616 (GRCm39)	Y316C	probably damaging	Het
Cdk5rap2	ATGTG	ATG	4: 70,208,214 (GRCm39)		probably null	Het
Cfap69	G	T	5: 5,634,645 (GRCm39)		probably benign	Het
Chrnb4	T	C	9: 54,951,182 (GRCm39)	E27G	probably damaging	Het
Clec14a	A	G	12: 58,315,430 (GRCm39)	V64A	possibly damaging	Het
Crygs	A	G	16: 22,625,262 (GRCm39)	Y60H	probably damaging	Het
Dpp10	T	A	1: 123,281,216 (GRCm39)	Q611L	probably damaging	Het
Entpd1	T	C	19: 40,725,264 (GRCm39)	L450P	probably damaging	Het
Eogt	G	A	6: 97,097,151 (GRCm39)	S317L	probably damaging	Het
Exosc10	T	A	4: 148,657,376 (GRCm39)	S584T	probably benign	Het
Fam184a	C	T	10: 53,575,157 (GRCm39)	V151I	probably damaging	Het
Fbxo43	A	G	15: 36,152,258 (GRCm39)	V517A	probably damaging	Het
Flii	T	C	11: 60,610,698 (GRCm39)	T533A	possibly damaging	Het
Frmpd1	T	G	4: 45,284,093 (GRCm39)	H971Q	probably benign	Het
Gata3	A	T	2: 9,867,954 (GRCm39)	N333K	probably damaging	Het
Hectd1	A	G	12: 51,815,513 (GRCm39)	S1525P	probably damaging	Het
Ibtk	A	G	9: 85,600,479 (GRCm39)	I816T	probably benign	Het
Kirrel1	C	T	3: 86,996,458 (GRCm39)	M380I	probably null	Het
Krt34	T	C	11: 99,931,791 (GRCm39)	T143A	probably benign	Het
Lipn	G	T	19: 34,046,828 (GRCm39)	M43I	probably benign	Het
Lrp1b	A	G	2: 41,335,206 (GRCm39)	C779R	probably damaging	Het
Mios	G	A	6: 8,233,189 (GRCm39)	R779Q	probably damaging	Het
Mme	A	T	3: 63,269,480 (GRCm39)		probably benign	Het
Ncstn	A	G	1: 171,897,640 (GRCm39)	S418P	probably benign	Het
Or10d1c	T	A	9: 38,894,166 (GRCm39)	Y58F	probably damaging	Het
Or4c29	T	C	2: 88,739,952 (GRCm39)	T262A	possibly damaging	Het
Or4k39	T	C	2: 111,238,967 (GRCm39)	V69A	possibly damaging	Het
Or6c210	C	T	10: 129,496,143 (GRCm39)	P156L	probably benign	Het
Or6z7	G	A	7: 6,484,131 (GRCm39)	A8V	probably benign	Het
Pald1	T	A	10: 61,183,266 (GRCm39)	N323Y	probably damaging	Het
Pcdhac1	C	A	18: 37,224,945 (GRCm39)	A586E	probably damaging	Het
Pcnx2	C	T	8: 126,614,897 (GRCm39)	A185T	probably benign	Het
Pik3r1	G	A	13: 101,821,465 (GRCm39)	H430Y	probably benign	Het
Polr3b	G	A	10: 84,556,382 (GRCm39)		probably null	Het
Prl8a6	A	T	13: 27,617,015 (GRCm39)	L225*	probably null	Het
Psme4	T	C	11: 30,806,068 (GRCm39)	S89P	probably damaging	Het
Pygl	G	A	12: 70,248,113 (GRCm39)	T250I	probably damaging	Het
Rgs13	T	A	1: 144,016,528 (GRCm39)	K72*	probably null	Het
Ryr2	T	A	13: 11,603,045 (GRCm39)	I4514F	probably damaging	Het
Septin3	A	T	15: 82,170,002 (GRCm39)	D32V	probably damaging	Het
Sfrp2	C	T	3: 83,674,335 (GRCm39)	P163S	possibly damaging	Het
Spata31	A	G	13: 65,068,745 (GRCm39)	T298A	probably benign	Het
Stxbp2	T	C	8: 3,683,369 (GRCm39)		probably null	Het
Syne1	A	T	10: 5,002,345 (GRCm39)	M282K	possibly damaging	Het
Timd2	T	C	11: 46,561,847 (GRCm39)		probably null	Het
Tlr11	G	A	14: 50,600,611 (GRCm39)	V866I	probably benign	Het
Trappc10	T	C	10: 78,056,020 (GRCm39)		probably null	Het
Zfp512b	AG	AGG	2: 181,230,556 (GRCm39)		probably null	Het

Other mutations in Oprk1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00926:Oprk1	APN	1	5,669,128 (GRCm39)	missense	probably damaging	0.99
IGL02049:Oprk1	APN	1	5,669,067 (GRCm39)	missense	probably damaging	1.00
IGL02076:Oprk1	APN	1	5,672,512 (GRCm39)	missense	probably damaging	1.00
IGL02265:Oprk1	APN	1	5,672,871 (GRCm39)	missense	probably damaging	1.00
IGL02294:Oprk1	APN	1	5,672,610 (GRCm39)	missense	probably damaging	1.00
IGL02584:Oprk1	APN	1	5,668,827 (GRCm39)	missense	probably damaging	1.00
IGL03164:Oprk1	APN	1	5,669,087 (GRCm39)	missense	probably damaging	1.00
R0295:Oprk1	UTSW	1	5,669,073 (GRCm39)	missense	possibly damaging	0.78
R1209:Oprk1	UTSW	1	5,672,484 (GRCm39)	missense	probably benign	0.00
R1420:Oprk1	UTSW	1	5,672,544 (GRCm39)	missense	probably damaging	1.00
R2994:Oprk1	UTSW	1	5,672,955 (GRCm39)	missense	probably benign	0.00
R4026:Oprk1	UTSW	1	5,668,908 (GRCm39)	missense	probably benign	0.04
R4096:Oprk1	UTSW	1	5,673,034 (GRCm39)	utr 3 prime	probably benign
R4097:Oprk1	UTSW	1	5,673,034 (GRCm39)	utr 3 prime	probably benign
R4475:Oprk1	UTSW	1	5,672,824 (GRCm39)	nonsense	probably null
R5177:Oprk1	UTSW	1	5,672,897 (GRCm39)	missense	probably damaging	1.00
R5223:Oprk1	UTSW	1	5,659,519 (GRCm39)	missense	probably benign	0.30
R6397:Oprk1	UTSW	1	5,668,971 (GRCm39)	missense	probably damaging	1.00
R6647:Oprk1	UTSW	1	5,672,507 (GRCm39)	missense	probably damaging	1.00
R7169:Oprk1	UTSW	1	5,659,304 (GRCm39)	missense	probably benign
R7170:Oprk1	UTSW	1	5,672,619 (GRCm39)	missense	probably damaging	1.00
R8186:Oprk1	UTSW	1	5,672,540 (GRCm39)	missense	probably benign	0.16
R9712:Oprk1	UTSW	1	5,669,096 (GRCm39)	missense	probably damaging	0.98
Z1176:Oprk1	UTSW	1	5,672,925 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GCAGTCTTCATCATCTGTTGGAC -3'
(R):5'- ACCTTTCCACTTCAGACTGCAG -3'

Sequencing Primer
(F):5'- GTTGGACCCCCATTCACATC -3'
(R):5'- CACTTCAGACTGCAGTTGTAATCTGG -3'

Posted On

2015-04-06