Incidental Mutation 'R3876:Oprk1'
ID 276823
Institutional Source Beutler Lab
Gene Symbol Oprk1
Ensembl Gene ENSMUSG00000025905
Gene Name opioid receptor, kappa 1
Synonyms R21, KOR-1, Oprk2
MMRRC Submission 041606-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.155) question?
Stock # R3876 (G1)
Quality Score 225
Status Validated
Chromosome 1
Chromosomal Location 5588466-5606131 bp(+) (GRCm38)
Type of Mutation nonsense
DNA Base Change (assembly) T to A at 5602661 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Cysteine to Stop codon at position 340 (C340*)
Ref Sequence ENSEMBL: ENSMUSP00000125105 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000027038] [ENSMUST00000160339] [ENSMUST00000160777]
AlphaFold P33534
Predicted Effect probably null
Transcript: ENSMUST00000027038
AA Change: C340*
SMART Domains Protein: ENSMUSP00000027038
Gene: ENSMUSG00000025905
AA Change: C340*

DomainStartEndE-ValueType
Pfam:7TM_GPCR_Srx 67 280 4.8e-8 PFAM
Pfam:7TM_GPCR_Srsx 70 345 5.4e-14 PFAM
Pfam:7tm_1 76 330 2.6e-67 PFAM
Pfam:7TM_GPCR_Srv 79 345 6.8e-9 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000159083
Predicted Effect probably null
Transcript: ENSMUST00000160339
AA Change: C340*
SMART Domains Protein: ENSMUSP00000124030
Gene: ENSMUSG00000025905
AA Change: C340*

DomainStartEndE-ValueType
Pfam:7TM_GPCR_Srx 67 284 1.1e-7 PFAM
Pfam:7TM_GPCR_Srsx 70 345 5.4e-14 PFAM
Pfam:7tm_1 76 330 2.6e-60 PFAM
Pfam:7TM_GPCR_Srv 78 345 5.2e-9 PFAM
Predicted Effect probably null
Transcript: ENSMUST00000160777
AA Change: C340*
SMART Domains Protein: ENSMUSP00000125105
Gene: ENSMUSG00000025905
AA Change: C340*

DomainStartEndE-ValueType
Pfam:7TM_GPCR_Srx 67 280 4.8e-8 PFAM
Pfam:7TM_GPCR_Srsx 70 345 5.4e-14 PFAM
Pfam:7tm_1 76 330 2.6e-67 PFAM
Pfam:7TM_GPCR_Srv 79 345 6.8e-9 PFAM
Meta Mutation Damage Score 0.9755 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.7%
  • 10x: 97.5%
  • 20x: 95.9%
Validation Efficiency 100% (53/53)
MGI Phenotype FUNCTION: This gene encodes an opioid receptor, which is a member of the 7 transmembrane-spanning G protein-coupled receptor family. It functions as a receptor for endogenous ligands, as well as a receptor for various synthetic opioids. Ligand binding results in inhibition of adenylate cyclase activity and neurotransmitter release. This opioid receptor plays a role in the perception of pain and mediating the hypolocomotor, analgesic and aversive actions of synthetic opioids. Variations in this gene have also been associated with alcohol dependence and opiate addiction. Alternatively spliced transcript variants have been found for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Jan 2016]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit impaired response to morphine and an opioid agonist, abnormal pain threshold, and increased litter size. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 50 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4931414P19Rik G A 14: 54,591,400 (GRCm38) R215* probably null Het
Brinp2 C A 1: 158,246,846 (GRCm38) L568F probably damaging Het
Brip1 T C 11: 86,152,790 (GRCm38) Y316C probably damaging Het
Cdk5rap2 ATGTG ATG 4: 70,289,977 (GRCm38) probably null Het
Cfap69 G T 5: 5,584,645 (GRCm38) probably benign Het
Chrnb4 T C 9: 55,043,898 (GRCm38) E27G probably damaging Het
Clec14a A G 12: 58,268,644 (GRCm38) V64A possibly damaging Het
Crygs A G 16: 22,806,512 (GRCm38) Y60H probably damaging Het
Dpp10 T A 1: 123,353,487 (GRCm38) Q611L probably damaging Het
Entpd1 T C 19: 40,736,820 (GRCm38) L450P probably damaging Het
Eogt G A 6: 97,120,190 (GRCm38) S317L probably damaging Het
Exosc10 T A 4: 148,572,919 (GRCm38) S584T probably benign Het
Fam184a C T 10: 53,699,061 (GRCm38) V151I probably damaging Het
Fbxo43 A G 15: 36,152,112 (GRCm38) V517A probably damaging Het
Flii T C 11: 60,719,872 (GRCm38) T533A possibly damaging Het
Frmpd1 T G 4: 45,284,093 (GRCm38) H971Q probably benign Het
Gata3 A T 2: 9,863,143 (GRCm38) N333K probably damaging Het
Hectd1 A G 12: 51,768,730 (GRCm38) S1525P probably damaging Het
Ibtk A G 9: 85,718,426 (GRCm38) I816T probably benign Het
Kirrel C T 3: 87,089,151 (GRCm38) M380I probably null Het
Krt34 T C 11: 100,040,965 (GRCm38) T143A probably benign Het
Lipn G T 19: 34,069,428 (GRCm38) M43I probably benign Het
Lrp1b A G 2: 41,445,194 (GRCm38) C779R probably damaging Het
Mios G A 6: 8,233,189 (GRCm38) R779Q probably damaging Het
Mme A T 3: 63,362,059 (GRCm38) probably benign Het
Ncstn A G 1: 172,070,073 (GRCm38) S418P probably benign Het
Olfr1209 T C 2: 88,909,608 (GRCm38) T262A possibly damaging Het
Olfr1285 T C 2: 111,408,622 (GRCm38) V69A possibly damaging Het
Olfr5 G A 7: 6,481,132 (GRCm38) A8V probably benign Het
Olfr800 C T 10: 129,660,274 (GRCm38) P156L probably benign Het
Olfr934 T A 9: 38,982,870 (GRCm38) Y58F probably damaging Het
Pald1 T A 10: 61,347,487 (GRCm38) N323Y probably damaging Het
Pcdhac1 C A 18: 37,091,892 (GRCm38) A586E probably damaging Het
Pcnx2 C T 8: 125,888,158 (GRCm38) A185T probably benign Het
Pik3r1 G A 13: 101,684,957 (GRCm38) H430Y probably benign Het
Polr3b G A 10: 84,720,518 (GRCm38) probably null Het
Prl8a6 A T 13: 27,433,032 (GRCm38) L225* probably null Het
Psme4 T C 11: 30,856,068 (GRCm38) S89P probably damaging Het
Pygl G A 12: 70,201,339 (GRCm38) T250I probably damaging Het
Rgs13 T A 1: 144,140,790 (GRCm38) K72* probably null Het
Ryr2 T A 13: 11,588,159 (GRCm38) I4514F probably damaging Het
Sept3 A T 15: 82,285,801 (GRCm38) D32V probably damaging Het
Sfrp2 C T 3: 83,767,028 (GRCm38) P163S possibly damaging Het
Spata31 A G 13: 64,920,931 (GRCm38) T298A probably benign Het
Stxbp2 T C 8: 3,633,369 (GRCm38) probably null Het
Syne1 A T 10: 5,052,345 (GRCm38) M282K possibly damaging Het
Timd2 T C 11: 46,671,020 (GRCm38) probably null Het
Tlr11 G A 14: 50,363,154 (GRCm38) V866I probably benign Het
Trappc10 T C 10: 78,220,186 (GRCm38) probably null Het
Zfp512b AG AGG 2: 181,588,763 (GRCm38) probably null Het
Other mutations in Oprk1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00926:Oprk1 APN 1 5,598,905 (GRCm38) missense probably damaging 0.99
IGL02049:Oprk1 APN 1 5,598,844 (GRCm38) missense probably damaging 1.00
IGL02076:Oprk1 APN 1 5,602,289 (GRCm38) missense probably damaging 1.00
IGL02265:Oprk1 APN 1 5,602,648 (GRCm38) missense probably damaging 1.00
IGL02294:Oprk1 APN 1 5,602,387 (GRCm38) missense probably damaging 1.00
IGL02584:Oprk1 APN 1 5,598,604 (GRCm38) missense probably damaging 1.00
IGL03164:Oprk1 APN 1 5,598,864 (GRCm38) missense probably damaging 1.00
R0295:Oprk1 UTSW 1 5,598,850 (GRCm38) missense possibly damaging 0.78
R1209:Oprk1 UTSW 1 5,602,261 (GRCm38) missense probably benign 0.00
R1420:Oprk1 UTSW 1 5,602,321 (GRCm38) missense probably damaging 1.00
R2994:Oprk1 UTSW 1 5,602,732 (GRCm38) missense probably benign 0.00
R4026:Oprk1 UTSW 1 5,598,685 (GRCm38) missense probably benign 0.04
R4096:Oprk1 UTSW 1 5,602,811 (GRCm38) utr 3 prime probably benign
R4097:Oprk1 UTSW 1 5,602,811 (GRCm38) utr 3 prime probably benign
R4475:Oprk1 UTSW 1 5,602,601 (GRCm38) nonsense probably null
R5177:Oprk1 UTSW 1 5,602,674 (GRCm38) missense probably damaging 1.00
R5223:Oprk1 UTSW 1 5,589,296 (GRCm38) missense probably benign 0.30
R6397:Oprk1 UTSW 1 5,598,748 (GRCm38) missense probably damaging 1.00
R6647:Oprk1 UTSW 1 5,602,284 (GRCm38) missense probably damaging 1.00
R7169:Oprk1 UTSW 1 5,589,081 (GRCm38) missense probably benign
R7170:Oprk1 UTSW 1 5,602,396 (GRCm38) missense probably damaging 1.00
R8186:Oprk1 UTSW 1 5,602,317 (GRCm38) missense probably benign 0.16
R9712:Oprk1 UTSW 1 5,598,873 (GRCm38) missense probably damaging 0.98
Z1176:Oprk1 UTSW 1 5,602,702 (GRCm38) missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- GCAGTCTTCATCATCTGTTGGAC -3'
(R):5'- ACCTTTCCACTTCAGACTGCAG -3'

Sequencing Primer
(F):5'- GTTGGACCCCCATTCACATC -3'
(R):5'- CACTTCAGACTGCAGTTGTAATCTGG -3'
Posted On 2015-04-06