Mutagenetix > Incidental Mutation

Incidental Mutation 'R3876:Tlr11'

276867

Institutional Source

Beutler Lab

Gene Symbol

Tlr11

Ensembl Gene

ENSMUSG00000051969

Gene Name

toll-like receptor 11

Synonyms

LOC239081

MMRRC Submission

041606-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3876 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

50357914-50363663 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 50363154 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Isoleucine at position 866 (V866I)

Ref Sequence

ENSEMBL: ENSMUSP00000138814 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000063570] [ENSMUST00000185091]

AlphaFold

Q6R5P0

Predicted Effect

probably benign
Transcript: ENSMUST00000063570
AA Change: V861I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000068906
Gene: ENSMUSG00000051969
AA Change: V861I

Domain	Start	End	E-Value	Type
signal peptide	1	30	N/A	INTRINSIC
low complexity region	105	122	N/A	INTRINSIC
low complexity region	153	161	N/A	INTRINSIC
LRR	311	333	3.36e1	SMART
LRR	335	361	4.44e0	SMART
LRR	362	383	2.03e1	SMART
LRR_TYP	384	407	2.57e-3	SMART
LRR_TYP	408	431	2.75e-3	SMART
low complexity region	544	556	N/A	INTRINSIC
LRR	605	628	6.06e1	SMART
transmembrane domain	719	741	N/A	INTRINSIC
Pfam:TIR	773	922	2.1e-9	PFAM
Pfam:TIR_2	776	894	6.6e-13	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000185091
AA Change: V866I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000138814
Gene: ENSMUSG00000051969
AA Change: V866I

Domain	Start	End	E-Value	Type
transmembrane domain	16	38	N/A	INTRINSIC
low complexity region	110	127	N/A	INTRINSIC
low complexity region	158	166	N/A	INTRINSIC
Pfam:LRR_6	221	244	5.3e-2	PFAM
LRR	316	338	3.36e1	SMART
LRR	340	366	4.44e0	SMART
LRR	367	388	2.03e1	SMART
LRR_TYP	389	412	2.57e-3	SMART
LRR_TYP	413	436	2.75e-3	SMART
low complexity region	549	561	N/A	INTRINSIC
LRR	610	633	6.06e1	SMART
transmembrane domain	724	746	N/A	INTRINSIC
Pfam:TIR_2	781	898	1e-12	PFAM
Pfam:TIR	781	922	1.8e-13	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.5%
20x: 95.9%

Validation Efficiency

100% (53/53)

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4931414P19Rik	G	A	14: 54,591,400	R215*	probably null	Het
Brinp2	C	A	1: 158,246,846	L568F	probably damaging	Het
Brip1	T	C	11: 86,152,790	Y316C	probably damaging	Het
Cdk5rap2	ATGTG	ATG	4: 70,289,977		probably null	Het
Cfap69	G	T	5: 5,584,645		probably benign	Het
Chrnb4	T	C	9: 55,043,898	E27G	probably damaging	Het
Clec14a	A	G	12: 58,268,644	V64A	possibly damaging	Het
Crygs	A	G	16: 22,806,512	Y60H	probably damaging	Het
Dpp10	T	A	1: 123,353,487	Q611L	probably damaging	Het
Entpd1	T	C	19: 40,736,820	L450P	probably damaging	Het
Eogt	G	A	6: 97,120,190	S317L	probably damaging	Het
Exosc10	T	A	4: 148,572,919	S584T	probably benign	Het
Fam184a	C	T	10: 53,699,061	V151I	probably damaging	Het
Fbxo43	A	G	15: 36,152,112	V517A	probably damaging	Het
Flii	T	C	11: 60,719,872	T533A	possibly damaging	Het
Frmpd1	T	G	4: 45,284,093	H971Q	probably benign	Het
Gata3	A	T	2: 9,863,143	N333K	probably damaging	Het
Hectd1	A	G	12: 51,768,730	S1525P	probably damaging	Het
Ibtk	A	G	9: 85,718,426	I816T	probably benign	Het
Kirrel	C	T	3: 87,089,151	M380I	probably null	Het
Krt34	T	C	11: 100,040,965	T143A	probably benign	Het
Lipn	G	T	19: 34,069,428	M43I	probably benign	Het
Lrp1b	A	G	2: 41,445,194	C779R	probably damaging	Het
Mios	G	A	6: 8,233,189	R779Q	probably damaging	Het
Mme	A	T	3: 63,362,059		probably benign	Het
Ncstn	A	G	1: 172,070,073	S418P	probably benign	Het
Olfr1209	T	C	2: 88,909,608	T262A	possibly damaging	Het
Olfr1285	T	C	2: 111,408,622	V69A	possibly damaging	Het
Olfr5	G	A	7: 6,481,132	A8V	probably benign	Het
Olfr800	C	T	10: 129,660,274	P156L	probably benign	Het
Olfr934	T	A	9: 38,982,870	Y58F	probably damaging	Het
Oprk1	T	A	1: 5,602,661	C340*	probably null	Het
Pald1	T	A	10: 61,347,487	N323Y	probably damaging	Het
Pcdhac1	C	A	18: 37,091,892	A586E	probably damaging	Het
Pcnx2	C	T	8: 125,888,158	A185T	probably benign	Het
Pik3r1	G	A	13: 101,684,957	H430Y	probably benign	Het
Polr3b	G	A	10: 84,720,518		probably null	Het
Prl8a6	A	T	13: 27,433,032	L225*	probably null	Het
Psme4	T	C	11: 30,856,068	S89P	probably damaging	Het
Pygl	G	A	12: 70,201,339	T250I	probably damaging	Het
Rgs13	T	A	1: 144,140,790	K72*	probably null	Het
Ryr2	T	A	13: 11,588,159	I4514F	probably damaging	Het
Sept3	A	T	15: 82,285,801	D32V	probably damaging	Het
Sfrp2	C	T	3: 83,767,028	P163S	possibly damaging	Het
Spata31	A	G	13: 64,920,931	T298A	probably benign	Het
Stxbp2	T	C	8: 3,633,369		probably null	Het
Syne1	A	T	10: 5,052,345	M282K	possibly damaging	Het
Timd2	T	C	11: 46,671,020		probably null	Het
Trappc10	T	C	10: 78,220,186		probably null	Het
Zfp512b	AG	AGG	2: 181,588,763		probably null	Het

Other mutations in Tlr11

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00590:Tlr11	APN	14	50360916	missense	probably benign
IGL02090:Tlr11	APN	14	50363032	missense	probably damaging	0.99
IGL02286:Tlr11	APN	14	50360871	missense	possibly damaging	0.91
IGL02671:Tlr11	APN	14	50360692	missense	probably damaging	1.00
IGL03064:Tlr11	APN	14	50361100	missense	probably damaging	1.00
IGL03068:Tlr11	APN	14	50361484	missense	probably benign
R0099:Tlr11	UTSW	14	50360818	missense	probably benign	0.14
R0727:Tlr11	UTSW	14	50361469	missense	possibly damaging	0.67
R0944:Tlr11	UTSW	14	50362336	missense	probably benign	0.12
R1490:Tlr11	UTSW	14	50363176	missense	probably benign	0.00
R1726:Tlr11	UTSW	14	50361541	missense	probably benign	0.00
R1803:Tlr11	UTSW	14	50360647	missense	probably benign	0.00
R1908:Tlr11	UTSW	14	50361207	missense	probably benign	0.00
R1971:Tlr11	UTSW	14	50361234	missense	probably benign
R1981:Tlr11	UTSW	14	50361988	missense	possibly damaging	0.95
R2023:Tlr11	UTSW	14	50362569	missense	probably damaging	0.96
R2079:Tlr11	UTSW	14	50360980	missense	probably damaging	0.99
R2155:Tlr11	UTSW	14	50360682	missense	probably benign	0.01
R2251:Tlr11	UTSW	14	50360792	missense	probably benign	0.02
R3017:Tlr11	UTSW	14	50362721	nonsense	probably null
R3760:Tlr11	UTSW	14	50362243	missense	probably damaging	1.00
R3936:Tlr11	UTSW	14	50362735	missense	possibly damaging	0.78
R4002:Tlr11	UTSW	14	50362527	missense	probably benign
R4024:Tlr11	UTSW	14	50362846	missense	probably benign	0.02
R4118:Tlr11	UTSW	14	50363227	missense	probably damaging	1.00
R4222:Tlr11	UTSW	14	50361849	missense	probably damaging	0.99
R4365:Tlr11	UTSW	14	50361469	missense	probably damaging	0.98
R4678:Tlr11	UTSW	14	50360982	missense	possibly damaging	0.85
R4779:Tlr11	UTSW	14	50361250	missense	possibly damaging	0.76
R4910:Tlr11	UTSW	14	50362889	missense	probably benign	0.45
R4921:Tlr11	UTSW	14	50362885	missense	possibly damaging	0.48
R5114:Tlr11	UTSW	14	50363121	missense	possibly damaging	0.81
R5126:Tlr11	UTSW	14	50360830	missense	probably damaging	1.00
R5349:Tlr11	UTSW	14	50360880	missense	probably benign	0.45
R5606:Tlr11	UTSW	14	50362260	missense	probably benign	0.08
R5650:Tlr11	UTSW	14	50361201	missense	probably benign	0.03
R5958:Tlr11	UTSW	14	50360777	missense	probably damaging	0.99
R5966:Tlr11	UTSW	14	50362255	missense	probably benign	0.02
R6480:Tlr11	UTSW	14	50363055	missense	possibly damaging	0.62
R6484:Tlr11	UTSW	14	50362678	missense	probably damaging	0.99
R6679:Tlr11	UTSW	14	50362854	missense	probably benign	0.00
R6717:Tlr11	UTSW	14	50362104	missense	probably benign
R7085:Tlr11	UTSW	14	50362656	missense	probably damaging	0.99
R7241:Tlr11	UTSW	14	50362141	missense	possibly damaging	0.95
R7440:Tlr11	UTSW	14	50361344	missense	probably benign	0.00
R7482:Tlr11	UTSW	14	50362999	missense	probably damaging	0.99
R7582:Tlr11	UTSW	14	50361729	nonsense	probably null
R7790:Tlr11	UTSW	14	50361925	missense	probably benign
R7818:Tlr11	UTSW	14	50361828	missense	probably damaging	1.00
R7827:Tlr11	UTSW	14	50361154	missense	probably benign	0.00
R8144:Tlr11	UTSW	14	50362488	missense	probably damaging	0.99
R8847:Tlr11	UTSW	14	50362725	missense	possibly damaging	0.85
R9027:Tlr11	UTSW	14	50361292	missense	probably damaging	1.00
R9035:Tlr11	UTSW	14	50360977	missense	probably benign	0.00
R9393:Tlr11	UTSW	14	50362090	missense	probably benign	0.03
RF002:Tlr11	UTSW	14	50361225	missense	possibly damaging	0.63
Z1088:Tlr11	UTSW	14	50362338	missense	possibly damaging	0.48
Z1176:Tlr11	UTSW	14	50360662	missense	probably damaging	1.00
Z1176:Tlr11	UTSW	14	50362336	missense	probably benign	0.40

Predicted Primers

PCR Primer
(F):5'- TCCTGAAGGTGAAGGCTTGAG -3'
(R):5'- TCTGCACAACAGCATTCCTGC -3'

Sequencing Primer
(F):5'- AAGGCTTGAGGTTGTGCC -3'
(R):5'- AGCTTTGCTGAGCCGTC -3'

Posted On

2015-04-06