Mutagenetix > Incidental Mutations

Incidental Mutation 'R3838:Zfp618'

276977

Institutional Source

Beutler Lab

Gene Symbol

Zfp618

Ensembl Gene

ENSMUSG00000028358

Gene Name

zinc finger protein 618

Synonyms

D430033D05Rik, 2810040O04Rik, 2810031P15Rik, Nedd10

MMRRC Submission

040779-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.172) question?

Stock #

R3838 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

62965573-63139708 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 63133564 bp

Zygosity

Heterozygous

Amino Acid Change

Alanine to Threonine at position 861 (A861T)

Ref Sequence

ENSEMBL: ENSMUSP00000103038 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000064814] [ENSMUST00000107415]

Predicted Effect

probably benign
Transcript: ENSMUST00000064814
AA Change: A768T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000069275
Gene: ENSMUSG00000028358
AA Change: A768T

Domain	Start	End	E-Value	Type
ZnF_C2H2	114	136	5.06e-2	SMART
ZnF_C2H2	155	177	8.81e-2	SMART
ZnF_C2H2	243	265	2.91e-2	SMART
low complexity region	288	295	N/A	INTRINSIC
ZnF_C2H2	298	320	2.53e-2	SMART
PDB:2BW3\|A	377	690	5e-8	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000107415
AA Change: A861T

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000103038
Gene: ENSMUSG00000028358
AA Change: A861T

Domain	Start	End	E-Value	Type
ZnF_C2H2	146	168	5.06e-2	SMART
ZnF_C2H2	187	209	8.81e-2	SMART
ZnF_C2H2	255	277	2.91e-2	SMART
low complexity region	381	388	N/A	INTRINSIC
ZnF_C2H2	391	413	2.53e-2	SMART
PDB:2BW3\|A	479	783	9e-8	PDB

Meta Mutation Damage Score

0.0723

Coding Region Coverage

1x: 99.3%
3x: 98.8%
10x: 97.8%
20x: 96.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700042G07Rik	A	G	4: 116,173,521	T41A	probably benign	Het
4930486L24Rik	A	G	13: 60,845,227	Y213H	probably damaging	Het
Alg8	C	T	7: 97,388,545	H379Y	probably damaging	Het
Arhgef25	T	C	10: 127,189,736	T12A	probably benign	Het
Arid4a	A	G	12: 71,075,785	E980G	possibly damaging	Het
Aspm	C	T	1: 139,478,054	H1560Y	probably benign	Het
Atg10	A	T	13: 90,937,380	I150K	probably damaging	Het
Bud13	A	C	9: 46,290,192	Q387P	possibly damaging	Het
Champ1	A	G	8: 13,879,939	Y699C	probably damaging	Het
Clstn1	A	G	4: 149,638,333	E476G	probably damaging	Het
Col2a1	T	C	15: 97,988,976	D345G	unknown	Het
Col2a1	A	T	15: 98,000,581		probably benign	Het
Col9a2	C	G	4: 121,054,258	R599G	probably damaging	Het
Dnajb2	G	A	1: 75,241,480		probably null	Het
Dock4	G	T	12: 40,794,624		probably null	Het
Epx	A	T	11: 87,874,830	L101Q	probably damaging	Het
F13a1	A	T	13: 37,047,424	N21K	probably damaging	Het
Fam13c	C	T	10: 70,542,648	S336L	probably damaging	Het
Fam35a	T	C	14: 34,245,368	D77G	probably benign	Het
Foxj3	T	A	4: 119,616,624	H215Q	possibly damaging	Het
Gcnt4	A	T	13: 96,947,014	R273*	probably null	Het
Gpam	T	C	19: 55,080,458	N450S	probably benign	Het
Hivep2	C	A	10: 14,128,969	T437K	probably benign	Het
Hmcn2	T	C	2: 31,413,407	L3020P	probably damaging	Het
Hmgcr	A	G	13: 96,659,089	I324T	probably benign	Het
Ighmbp2	T	C	19: 3,271,658	Y367C	probably benign	Het
Lrrc14b	A	G	13: 74,363,545	C139R	possibly damaging	Het
Lsamp	A	T	16: 42,134,312	E174V	possibly damaging	Het
Mid1ip1	T	C	X: 10,718,381	V51A	possibly damaging	Het
Mrc2	G	A	11: 105,348,431		probably null	Het
Msn	C	A	X: 96,160,199	Q303K	probably damaging	Het
Myh7b	A	G	2: 155,632,989	K1816E	probably damaging	Het
Nap1l1	T	A	10: 111,495,322		probably null	Het
Nme2	T	A	11: 93,949,977	E252D	probably benign	Het
Ntpcr	G	A	8: 125,737,372	V79M	probably damaging	Het
Ogdh	C	T	11: 6,338,627	R235*	probably null	Het
Olfr1490	A	G	19: 13,654,957	D176G	probably benign	Het
Olfr825	C	A	10: 130,162,406	E307*	probably null	Het
Olfr959	A	G	9: 39,572,971	V96A	probably benign	Het
Pcdh20	T	C	14: 88,468,463	N467S	probably benign	Het
Pkhd1	G	A	1: 20,534,629	T1154I	possibly damaging	Het
Polq	G	T	16: 37,078,349	R2157I	probably damaging	Het
Reep6	C	A	10: 80,335,889	A533E	probably damaging	Het
Senp2	T	C	16: 22,009,735	S32P	probably damaging	Het
Sept11	T	A	5: 93,148,399	I52N	probably damaging	Het
Slc17a4	C	T	13: 23,901,769	R387H	probably benign	Het
Spdye4b	C	T	5: 143,192,329	T11I	probably benign	Het
Srrt	C	T	5: 137,302,125		probably null	Het
Sspo	T	A	6: 48,480,820	C3085S	probably damaging	Het
Stim1	C	T	7: 102,411,296	T182I	possibly damaging	Het
Thbs2	C	A	17: 14,687,851	V217L	probably benign	Het
Thpo	G	A	16: 20,728,748	R38C	probably damaging	Het
Tmem210	A	G	2: 25,288,432	E35G	possibly damaging	Het
Trim12c	T	A	7: 104,340,868		probably benign	Het
Tvp23b	A	G	11: 62,883,629	H33R	possibly damaging	Het
Usp14	A	G	18: 10,024,532		probably null	Het
Vmn2r73	A	G	7: 85,858,050	W685R	probably benign	Het
Zfp715	A	G	7: 43,299,756	V260A	probably benign	Het

Other mutations in Zfp618

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01301:Zfp618	APN	4	63132826	missense	probably damaging	1.00
IGL01563:Zfp618	APN	4	63079896	missense	probably benign	0.38
IGL01726:Zfp618	APN	4	63132635	missense	probably damaging	1.00
IGL02139:Zfp618	APN	4	63133536	missense	probably damaging	1.00
IGL02182:Zfp618	APN	4	63095561	splice site	probably benign
IGL02533:Zfp618	APN	4	63089405	missense	probably damaging	1.00
IGL03231:Zfp618	APN	4	63094479	missense	probably damaging	1.00
IGL03257:Zfp618	APN	4	63132671	missense	probably damaging	1.00
ANU18:Zfp618	UTSW	4	63132826	missense	probably damaging	1.00
IGL03014:Zfp618	UTSW	4	63080088	missense	probably damaging	1.00
R0288:Zfp618	UTSW	4	63132934	missense	possibly damaging	0.57
R0408:Zfp618	UTSW	4	63086572	missense	probably damaging	0.97
R0685:Zfp618	UTSW	4	63133774	missense	probably benign	0.21
R1482:Zfp618	UTSW	4	63115448	missense	possibly damaging	0.64
R1585:Zfp618	UTSW	4	63132938	missense	probably damaging	1.00
R1649:Zfp618	UTSW	4	63095537	missense	probably damaging	1.00
R1744:Zfp618	UTSW	4	63086634	splice site	probably benign
R1793:Zfp618	UTSW	4	63133237	missense	probably damaging	0.97
R1952:Zfp618	UTSW	4	63132318	splice site	probably null
R1996:Zfp618	UTSW	4	63131215	splice site	probably null
R3792:Zfp618	UTSW	4	63115491	intron	probably benign
R3803:Zfp618	UTSW	4	63133019	missense	probably damaging	1.00
R3821:Zfp618	UTSW	4	63133564	missense	probably benign	0.00
R4009:Zfp618	UTSW	4	63133564	missense	probably benign	0.00
R4010:Zfp618	UTSW	4	63133564	missense	probably benign	0.00
R4565:Zfp618	UTSW	4	63121351	missense	probably damaging	1.00
R4611:Zfp618	UTSW	4	63132979	missense	probably damaging	1.00
R5019:Zfp618	UTSW	4	63103552	missense	probably damaging	1.00
R5154:Zfp618	UTSW	4	63133209	missense	probably damaging	1.00
R5183:Zfp618	UTSW	4	63099282	missense	probably benign
R5354:Zfp618	UTSW	4	63080028	missense	probably damaging	1.00
R5383:Zfp618	UTSW	4	63095492	missense	probably benign	0.33
R5774:Zfp618	UTSW	4	63132562	missense	probably damaging	1.00
R5932:Zfp618	UTSW	4	63118566	nonsense	probably null
R6101:Zfp618	UTSW	4	63133241	missense	probably benign	0.09
R6105:Zfp618	UTSW	4	63133241	missense	probably benign	0.09
R6478:Zfp618	UTSW	4	63132706	missense	probably damaging	1.00
R6598:Zfp618	UTSW	4	63089399	missense	probably damaging	1.00
R7386:Zfp618	UTSW	4	63095385	critical splice donor site	probably null
R7666:Zfp618	UTSW	4	63132717	nonsense	probably null
R7678:Zfp618	UTSW	4	63086621	missense	probably benign	0.07
R7975:Zfp618	UTSW	4	63131115	missense	possibly damaging	0.93
R8276:Zfp618	UTSW	4	63132956	missense	probably damaging	1.00
R8421:Zfp618	UTSW	4	63133246	missense	probably damaging	1.00
X0011:Zfp618	UTSW	4	63080006	missense	probably damaging	0.99
Z1176:Zfp618	UTSW	4	63095497	missense	probably benign	0.29
Z1176:Zfp618	UTSW	4	63132763	missense	probably benign	0.12

Predicted Primers

PCR Primer
(F):5'- GCTCAAGGAGAACTTCAAGGTG -3'
(R):5'- ATTTACACACCCGCTCCGAG -3'

Sequencing Primer
(F):5'- GGAGAACTTCAAGGTGCACCC -3'
(R):5'- GAGCCCCGACAGCAGGAAC -3'

Posted On

2015-04-06