Mutagenetix > Incidental Mutation

Incidental Mutation 'R3838:Or9k2'

277005

Institutional Source

Beutler Lab

Gene Symbol

Or9k2

Ensembl Gene

ENSMUSG00000058084

Gene Name

olfactory receptor family 9 subfamily K member 2

Synonyms

GA_x6K02T2PULF-11834065-11833103, Olfr825, MOR210-1

MMRRC Submission

040779-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.370) question?

Stock #

R3838 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

129998231-129999193 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 129998275 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Stop codon at position 307 (E307*)

Ref Sequence

ENSEMBL: ENSMUSP00000149981 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076814] [ENSMUST00000216530]

AlphaFold

Q8VFU7

Predicted Effect

probably null
Transcript: ENSMUST00000076814
AA Change: E307*

SMART Domains

Protein: ENSMUSP00000076091
Gene: ENSMUSG00000058084
AA Change: E307*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	34	309	8.8e-54	PFAM
Pfam:7TM_GPCR_Srsx	38	251	9.6e-6	PFAM
Pfam:7tm_1	44	291	2e-22	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000216434

Predicted Effect

probably null
Transcript: ENSMUST00000216530
AA Change: E307*

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000218167

Coding Region Coverage

1x: 99.3%
3x: 98.8%
10x: 97.8%
20x: 96.4%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930486L24Rik	A	G	13: 60,993,041 (GRCm39)	Y213H	probably damaging	Het
Alg8	C	T	7: 97,037,752 (GRCm39)	H379Y	probably damaging	Het
Arhgef25	T	C	10: 127,025,605 (GRCm39)	T12A	probably benign	Het
Arid4a	A	G	12: 71,122,559 (GRCm39)	E980G	possibly damaging	Het
Aspm	C	T	1: 139,405,792 (GRCm39)	H1560Y	probably benign	Het
Atg10	A	T	13: 91,085,499 (GRCm39)	I150K	probably damaging	Het
Bud13	A	C	9: 46,201,490 (GRCm39)	Q387P	possibly damaging	Het
Champ1	A	G	8: 13,929,939 (GRCm39)	Y699C	probably damaging	Het
Clstn1	A	G	4: 149,722,790 (GRCm39)	E476G	probably damaging	Het
Col2a1	T	C	15: 97,886,857 (GRCm39)	D345G	unknown	Het
Col2a1	A	T	15: 97,898,462 (GRCm39)		probably benign	Het
Col9a2	C	G	4: 120,911,455 (GRCm39)	R599G	probably damaging	Het
Dnajb2	G	A	1: 75,218,124 (GRCm39)		probably null	Het
Dock4	G	T	12: 40,844,623 (GRCm39)		probably null	Het
Epx	A	T	11: 87,765,656 (GRCm39)	L101Q	probably damaging	Het
F13a1	A	T	13: 37,231,398 (GRCm39)	N21K	probably damaging	Het
Fam13c	C	T	10: 70,378,478 (GRCm39)	S336L	probably damaging	Het
Foxj3	T	A	4: 119,473,821 (GRCm39)	H215Q	possibly damaging	Het
Gcnt4	A	T	13: 97,083,522 (GRCm39)	R273*	probably null	Het
Gpam	T	C	19: 55,068,890 (GRCm39)	N450S	probably benign	Het
Hivep2	C	A	10: 14,004,713 (GRCm39)	T437K	probably benign	Het
Hmcn2	T	C	2: 31,303,419 (GRCm39)	L3020P	probably damaging	Het
Hmgcr	A	G	13: 96,795,597 (GRCm39)	I324T	probably benign	Het
Ighmbp2	T	C	19: 3,321,658 (GRCm39)	Y367C	probably benign	Het
Lrrc14b	A	G	13: 74,511,664 (GRCm39)	C139R	possibly damaging	Het
Lsamp	A	T	16: 41,954,675 (GRCm39)	E174V	possibly damaging	Het
Mid1ip1	T	C	X: 10,584,620 (GRCm39)	V51A	possibly damaging	Het
Mrc2	G	A	11: 105,239,257 (GRCm39)		probably null	Het
Msn	C	A	X: 95,203,805 (GRCm39)	Q303K	probably damaging	Het
Myh7b	A	G	2: 155,474,909 (GRCm39)	K1816E	probably damaging	Het
Nap1l1	T	A	10: 111,331,183 (GRCm39)		probably null	Het
Nme2	T	A	11: 93,840,803 (GRCm39)	E252D	probably benign	Het
Ntpcr	G	A	8: 126,464,111 (GRCm39)	V79M	probably damaging	Het
Ogdh	C	T	11: 6,288,627 (GRCm39)	R235*	probably null	Het
Or10d1	A	G	9: 39,484,267 (GRCm39)	V96A	probably benign	Het
Or10w1	A	G	19: 13,632,321 (GRCm39)	D176G	probably benign	Het
P3r3urf	A	G	4: 116,030,718 (GRCm39)	T41A	probably benign	Het
Pcdh20	T	C	14: 88,705,899 (GRCm39)	N467S	probably benign	Het
Pkhd1	G	A	1: 20,604,853 (GRCm39)	T1154I	possibly damaging	Het
Polq	G	T	16: 36,898,711 (GRCm39)	R2157I	probably damaging	Het
Reep6	C	A	10: 80,171,723 (GRCm39)	A533E	probably damaging	Het
Senp2	T	C	16: 21,828,485 (GRCm39)	S32P	probably damaging	Het
Septin11	T	A	5: 93,296,258 (GRCm39)	I52N	probably damaging	Het
Shld2	T	C	14: 33,967,325 (GRCm39)	D77G	probably benign	Het
Slc17a4	C	T	13: 24,085,752 (GRCm39)	R387H	probably benign	Het
Spdye4b	C	T	5: 143,178,084 (GRCm39)	T11I	probably benign	Het
Srrt	C	T	5: 137,300,387 (GRCm39)		probably null	Het
Sspo	T	A	6: 48,457,754 (GRCm39)	C3085S	probably damaging	Het
Stim1	C	T	7: 102,060,503 (GRCm39)	T182I	possibly damaging	Het
Thbs2	C	A	17: 14,908,113 (GRCm39)	V217L	probably benign	Het
Thpo	G	A	16: 20,547,498 (GRCm39)	R38C	probably damaging	Het
Tmem210	A	G	2: 25,178,444 (GRCm39)	E35G	possibly damaging	Het
Trim12c	T	A	7: 103,990,075 (GRCm39)		probably benign	Het
Tvp23b	A	G	11: 62,774,455 (GRCm39)	H33R	possibly damaging	Het
Usp14	A	G	18: 10,024,532 (GRCm39)		probably null	Het
Vmn2r73	A	G	7: 85,507,258 (GRCm39)	W685R	probably benign	Het
Zfp618	G	A	4: 63,051,801 (GRCm39)	A861T	probably benign	Het
Zfp715	A	G	7: 42,949,180 (GRCm39)	V260A	probably benign	Het

Other mutations in Or9k2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03244:Or9k2	APN	10	129,998,269 (GRCm39)	nonsense	probably null
R1120:Or9k2	UTSW	10	129,998,406 (GRCm39)	missense	probably damaging	1.00
R1341:Or9k2	UTSW	10	129,999,185 (GRCm39)	missense	probably benign	0.00
R1754:Or9k2	UTSW	10	129,999,033 (GRCm39)	missense	probably benign	0.02
R2022:Or9k2	UTSW	10	129,999,049 (GRCm39)	missense	probably benign	0.00
R2027:Or9k2	UTSW	10	129,998,604 (GRCm39)	missense	probably benign	0.00
R3842:Or9k2	UTSW	10	129,998,770 (GRCm39)	missense	probably benign	0.00
R4737:Or9k2	UTSW	10	129,998,707 (GRCm39)	missense	probably benign	0.01
R5166:Or9k2	UTSW	10	129,998,430 (GRCm39)	missense	possibly damaging	0.60
R5744:Or9k2	UTSW	10	129,998,661 (GRCm39)	missense	possibly damaging	0.92
R5899:Or9k2	UTSW	10	129,998,542 (GRCm39)	missense	probably benign	0.00
R7754:Or9k2	UTSW	10	129,998,698 (GRCm39)	missense	probably damaging	1.00
R8098:Or9k2	UTSW	10	129,998,916 (GRCm39)	missense	probably benign	0.32
R9393:Or9k2	UTSW	10	129,999,016 (GRCm39)	missense	probably benign	0.39
X0026:Or9k2	UTSW	10	129,998,600 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CTCCCCAGGACATTGACATG -3'
(R):5'- AGAAAGCCTTCTCCACTTGC -3'

Sequencing Primer
(F):5'- ACATTGACATGTCACCGAATTTAAG -3'
(R):5'- ACTTGCAGCTCTCACCTGGG -3'

Posted On

2015-04-06