Mutagenetix > Incidental Mutation

Incidental Mutation 'R3847:Wnk1'

277428

Institutional Source

Beutler Lab

Gene Symbol

Wnk1

Ensembl Gene

ENSMUSG00000045962

Gene Name

WNK lysine deficient protein kinase 1

Synonyms

6430573H23Rik, Hsn2, EG406236, Prkwnk1

MMRRC Submission

040895-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R3847 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

119923969-120038672 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 119969354 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Serine at position 613 (G613S)

Ref Sequence

ENSEMBL: ENSMUSP00000145304 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000060043] [ENSMUST00000088644] [ENSMUST00000088646] [ENSMUST00000177761] [ENSMUST00000203030]

AlphaFold

P83741

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000035513

Predicted Effect

possibly damaging
Transcript: ENSMUST00000060043
AA Change: G613S

PolyPhen 2 Score 0.702 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000063001
Gene: ENSMUSG00000045962
AA Change: G613S

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.5e-44	PFAM
Pfam:Pkinase	221	479	4.4e-58	PFAM
Pfam:OSR1_C	500	537	2e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	865	876	N/A	INTRINSIC
low complexity region	1018	1028	N/A	INTRINSIC
low complexity region	1042	1058	N/A	INTRINSIC
internal_repeat_1	1136	1178	2.15e-5	PROSPERO
low complexity region	1289	1305	N/A	INTRINSIC
low complexity region	1345	1357	N/A	INTRINSIC
low complexity region	1379	1396	N/A	INTRINSIC
low complexity region	1398	1428	N/A	INTRINSIC
low complexity region	1429	1454	N/A	INTRINSIC
low complexity region	1496	1515	N/A	INTRINSIC
low complexity region	1542	1556	N/A	INTRINSIC
low complexity region	1586	1609	N/A	INTRINSIC
low complexity region	1670	1691	N/A	INTRINSIC
low complexity region	1698	1712	N/A	INTRINSIC
low complexity region	1738	1764	N/A	INTRINSIC
low complexity region	1882	1900	N/A	INTRINSIC
coiled coil region	2065	2092	N/A	INTRINSIC
low complexity region	2103	2114	N/A	INTRINSIC
low complexity region	2116	2140	N/A	INTRINSIC
low complexity region	2208	2232	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000088644
AA Change: G613S

PolyPhen 2 Score 0.702 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000086017
Gene: ENSMUSG00000045962
AA Change: G613S

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	5.5e-44	PFAM
Pfam:Pkinase	221	479	4.3e-56	PFAM
Pfam:OSR1_C	500	537	1.9e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	742	753	N/A	INTRINSIC
low complexity region	919	935	N/A	INTRINSIC
low complexity region	1050	1063	N/A	INTRINSIC
low complexity region	1067	1076	N/A	INTRINSIC
low complexity region	1146	1158	N/A	INTRINSIC
low complexity region	1276	1286	N/A	INTRINSIC
low complexity region	1300	1316	N/A	INTRINSIC
internal_repeat_1	1394	1436	2.19e-5	PROSPERO
low complexity region	1547	1563	N/A	INTRINSIC
low complexity region	1603	1615	N/A	INTRINSIC
low complexity region	1637	1654	N/A	INTRINSIC
low complexity region	1656	1686	N/A	INTRINSIC
low complexity region	1687	1712	N/A	INTRINSIC
low complexity region	1754	1773	N/A	INTRINSIC
low complexity region	1800	1814	N/A	INTRINSIC
low complexity region	1844	1867	N/A	INTRINSIC
low complexity region	1928	1949	N/A	INTRINSIC
low complexity region	1956	1970	N/A	INTRINSIC
low complexity region	1996	2022	N/A	INTRINSIC
low complexity region	2140	2158	N/A	INTRINSIC
coiled coil region	2323	2350	N/A	INTRINSIC
low complexity region	2361	2372	N/A	INTRINSIC
low complexity region	2374	2398	N/A	INTRINSIC
low complexity region	2466	2490	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000088646
AA Change: G613S

PolyPhen 2 Score 0.702 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000086019
Gene: ENSMUSG00000045962
AA Change: G613S

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.3e-44	PFAM
Pfam:Pkinase	221	479	4e-58	PFAM
Pfam:OSR1_C	500	537	1.8e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	864	874	N/A	INTRINSIC
low complexity region	888	904	N/A	INTRINSIC
internal_repeat_1	982	1024	7.56e-6	PROSPERO
low complexity region	1135	1151	N/A	INTRINSIC
low complexity region	1191	1203	N/A	INTRINSIC
low complexity region	1225	1242	N/A	INTRINSIC
low complexity region	1244	1274	N/A	INTRINSIC
low complexity region	1275	1300	N/A	INTRINSIC
low complexity region	1342	1361	N/A	INTRINSIC
low complexity region	1388	1402	N/A	INTRINSIC
low complexity region	1432	1455	N/A	INTRINSIC
low complexity region	1516	1537	N/A	INTRINSIC
low complexity region	1544	1558	N/A	INTRINSIC
low complexity region	1584	1610	N/A	INTRINSIC
low complexity region	1700	1718	N/A	INTRINSIC
coiled coil region	1883	1910	N/A	INTRINSIC
low complexity region	1921	1932	N/A	INTRINSIC
low complexity region	1934	1958	N/A	INTRINSIC
low complexity region	2026	2050	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000159151

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000159397

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000160769

Predicted Effect

unknown
Transcript: ENSMUST00000161048
AA Change: G201S

SMART Domains

Protein: ENSMUSP00000125345
Gene: ENSMUSG00000045962
AA Change: G201S

Domain	Start	End	E-Value	Type
Blast:S_TKc	2	68	3e-34	BLAST
SCOP:d1phk__	3	70	2e-7	SMART
Pfam:OSR1_C	89	126	9.5e-21	PFAM
coiled coil region	151	185	N/A	INTRINSIC
low complexity region	323	335	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000161392

Predicted Effect

probably benign
Transcript: ENSMUST00000162368

SMART Domains

Protein: ENSMUSP00000125383
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
low complexity region	83	95	N/A	INTRINSIC
low complexity region	142	158	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000177761
AA Change: G613S

PolyPhen 2 Score 0.702 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000136777
Gene: ENSMUSG00000045962
AA Change: G613S

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.7e-44	PFAM
Pfam:Pkinase	221	479	5.1e-58	PFAM
Pfam:OSR1_C	500	537	2.2e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	710	722	N/A	INTRINSIC
low complexity region	828	839	N/A	INTRINSIC
low complexity region	1005	1021	N/A	INTRINSIC
low complexity region	1136	1149	N/A	INTRINSIC
low complexity region	1153	1162	N/A	INTRINSIC
low complexity region	1232	1244	N/A	INTRINSIC
low complexity region	1291	1307	N/A	INTRINSIC
internal_repeat_1	1385	1427	1.91e-5	PROSPERO
low complexity region	1538	1554	N/A	INTRINSIC
low complexity region	1594	1606	N/A	INTRINSIC
low complexity region	1628	1645	N/A	INTRINSIC
low complexity region	1647	1677	N/A	INTRINSIC
low complexity region	1678	1703	N/A	INTRINSIC
low complexity region	1745	1764	N/A	INTRINSIC
low complexity region	1791	1805	N/A	INTRINSIC
low complexity region	1835	1858	N/A	INTRINSIC
low complexity region	1919	1940	N/A	INTRINSIC
low complexity region	1947	1961	N/A	INTRINSIC
low complexity region	1987	2013	N/A	INTRINSIC
low complexity region	2131	2149	N/A	INTRINSIC
coiled coil region	2314	2341	N/A	INTRINSIC
low complexity region	2352	2363	N/A	INTRINSIC
low complexity region	2365	2389	N/A	INTRINSIC
low complexity region	2457	2481	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000203030
AA Change: G613S

PolyPhen 2 Score 0.702 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000145304
Gene: ENSMUSG00000045962
AA Change: G613S

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	4.1e-44	PFAM
Pfam:Pkinase	221	479	3.2e-56	PFAM
Pfam:OSR1_C	500	537	1.5e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	793	809	N/A	INTRINSIC
internal_repeat_1	887	929	5.8e-6	PROSPERO
low complexity region	1040	1056	N/A	INTRINSIC
low complexity region	1096	1108	N/A	INTRINSIC
low complexity region	1130	1147	N/A	INTRINSIC
low complexity region	1149	1179	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1247	1266	N/A	INTRINSIC
low complexity region	1293	1307	N/A	INTRINSIC
low complexity region	1337	1360	N/A	INTRINSIC
low complexity region	1421	1442	N/A	INTRINSIC
low complexity region	1449	1463	N/A	INTRINSIC
low complexity region	1489	1515	N/A	INTRINSIC
low complexity region	1633	1651	N/A	INTRINSIC
coiled coil region	1816	1843	N/A	INTRINSIC
low complexity region	1854	1865	N/A	INTRINSIC
low complexity region	1867	1891	N/A	INTRINSIC
low complexity region	1959	1983	N/A	INTRINSIC

Meta Mutation Damage Score

0.0674

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.7%
20x: 96.1%

Validation Efficiency

100% (66/66)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq, May 2010]
PHENOTYPE: Homozygous mutant mice die before birth, whereas heterozygotes survive and exhibit decreased blood pressure. Mice homozygous for an allele that does not produce the kidney isoform exhibit a slight increase in systemic arterial diastolic blood pressure and reduced sensitivity to amiloride. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930522L14Rik	T	C	5: 109,736,324		probably null	Het
Abcc12	C	T	8: 86,553,391	E338K	probably benign	Het
Abcc5	A	T	16: 20,372,156	S815T	probably benign	Het
Ankrd16	G	A	2: 11,789,808	E335K	probably benign	Het
Atm	A	G	9: 53,503,075	F905S	possibly damaging	Het
Bckdha	C	T	7: 25,631,652	R281H	probably damaging	Het
Blvra	C	T	2: 127,095,191	T188I	probably damaging	Het
Bmp8b	C	A	4: 123,116,168		probably benign	Het
Cacna2d3	A	G	14: 29,347,120		probably null	Het
Cacng8	T	C	7: 3,394,474	S84P	probably damaging	Het
Cad	T	A	5: 31,061,650	V605E	probably damaging	Het
Ccny	A	G	18: 9,449,641	S11P	probably benign	Het
Col3a1	C	A	1: 45,321,990	P112T	unknown	Het
Corin	T	C	5: 72,422,165	E155G	probably benign	Het
Cul9	T	C	17: 46,525,135	Y1195C	probably damaging	Het
Cyp27a1	A	G	1: 74,737,559	E501G	probably damaging	Het
Dennd3	T	A	15: 73,542,732	N457K	possibly damaging	Het
Dnah7a	T	G	1: 53,501,656	I2520L	probably benign	Het
Dst	C	T	1: 34,212,319	S4165F	probably damaging	Het
Fgf14	A	C	14: 123,980,389	I234R	probably benign	Het
Foxp4	A	G	17: 47,875,528	I442T	unknown	Het
Gad2	T	G	2: 22,684,988	D472E	probably benign	Het
Garnl3	G	T	2: 32,992,228	H832N	probably benign	Het
Gm13941	T	C	2: 111,104,853	M11V	unknown	Het
Gnptab	T	A	10: 88,433,577	L714*	probably null	Het
Golgb1	C	A	16: 36,898,733	Q334K	probably benign	Het
Gp9	A	G	6: 87,779,151	I49M	probably benign	Het
Guf1	A	G	5: 69,561,157	N213S	probably damaging	Het
H2-K1	T	G	17: 33,997,329	H281P	probably damaging	Het
Hhip	T	A	8: 79,997,495	M373L	probably benign	Het
Ifi203	C	T	1: 173,933,796	C229Y	possibly damaging	Het
Ighv13-2	A	G	12: 114,357,798	L88S	probably damaging	Het
Kcna3	T	C	3: 107,036,696	Y92H	possibly damaging	Het
Lmo7	A	G	14: 101,922,095		probably null	Het
Lrguk	A	G	6: 34,073,768	D387G	probably damaging	Het
Mki67	A	C	7: 135,696,130	S2392A	probably benign	Het
Mknk2	C	T	10: 80,667,975	W367*	probably null	Het
Mocos	A	G	18: 24,676,662	K441E	probably damaging	Het
Mpnd	T	C	17: 56,011,692	S150P	probably damaging	Het
Naip2	A	T	13: 100,179,432	L280Q	probably damaging	Het
Naip2	G	C	13: 100,179,433	L280V	probably damaging	Het
Neurod4	C	T	10: 130,270,482	V308I	probably benign	Het
Nxf3	C	T	X: 136,073,983	V474I	probably benign	Het
Olfr1037	A	T	2: 86,085,407	Y123*	probably null	Het
Olfr976	A	T	9: 39,956,581	M118K	probably damaging	Het
Pam	T	A	1: 97,854,756		probably benign	Het
Pde5a	T	C	3: 122,803,160	Y467H	probably damaging	Het
Pibf1	T	A	14: 99,137,121	V332E	possibly damaging	Het
Plxna1	C	A	6: 89,356,519	R376L	probably damaging	Het
Proz	A	G	8: 13,073,533	E268G	probably benign	Het
Rimbp3	A	T	16: 17,210,299	H529L	probably benign	Het
Rnf103	C	G	6: 71,508,875	C163W	probably damaging	Het
Rnf17	T	C	14: 56,512,296	V1433A	probably damaging	Het
Sept11	G	T	5: 93,162,167	M276I	probably damaging	Het
Slc30a4	A	G	2: 122,702,272	V50A	probably damaging	Het
Sorbs1	A	G	19: 40,314,443	V570A	probably damaging	Het
Spout1	T	C	2: 30,177,407		probably null	Het
Syne2	T	C	12: 76,048,622	L5241P	probably damaging	Het
Tas2r110	T	A	6: 132,868,675	I223N	probably damaging	Het
Tead2	T	A	7: 45,232,328		probably null	Het
Thsd1	A	G	8: 22,259,411	H713R	probably damaging	Het
Ttc21b	A	G	2: 66,242,679	L221S	probably damaging	Het
Ugcg	C	T	4: 59,207,798	P46S	probably benign	Het
Vmn2r105	A	G	17: 20,208,690	I708T	possibly damaging	Het
Vmn2r117	G	T	17: 23,460,415	H612N	probably damaging	Het
Zbtb41	T	A	1: 139,423,996	H282Q	probably benign	Het
Zfp335	C	A	2: 164,900,106		probably null	Het
Zmym2	T	A	14: 56,921,499		probably benign	Het

Other mutations in Wnk1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00901:Wnk1	APN	6	119960708	missense	probably damaging	1.00
IGL01364:Wnk1	APN	6	119937411	missense	probably damaging	1.00
IGL01715:Wnk1	APN	6	119948397	missense	probably damaging	1.00
IGL01951:Wnk1	APN	6	119963485	missense	probably damaging	0.99
IGL02043:Wnk1	APN	6	119949078	unclassified	probably benign
IGL02268:Wnk1	APN	6	119937373	nonsense	probably null
IGL02348:Wnk1	APN	6	119963328	missense	probably damaging	0.98
IGL02425:Wnk1	APN	6	119963454	missense	probably damaging	1.00
IGL02850:Wnk1	APN	6	119937862	missense	probably benign	0.26
IGL03160:Wnk1	APN	6	119926633	missense	probably damaging	1.00
IGL03387:Wnk1	APN	6	119954187	missense	possibly damaging	0.76
IGL03405:Wnk1	APN	6	119953895	missense	probably benign	0.41
Actor	UTSW	6	119970978	missense	probably damaging	1.00
Bad	UTSW	6	119965678	intron	probably benign
Blink	UTSW	6	120002273	nonsense	probably null
Knock	UTSW	6	119943878	splice site	probably null
Narrow	UTSW	6	119992408	missense	probably damaging	1.00
nictitate	UTSW	6	120002280	missense	possibly damaging	0.88
opportunity	UTSW	6	119951068	missense	probably damaging	1.00
path	UTSW	6	120037149	missense	probably damaging	0.99
Stormy	UTSW	6	119992447	missense	probably damaging	1.00
tear	UTSW	6	119963470	missense	probably damaging	0.98
Tic	UTSW	6	119950083	missense	probably damaging	1.00
Unforgiving	UTSW	6	119948282	missense	probably damaging	1.00
Window	UTSW	6	119992453	missense	probably damaging	1.00
Woke	UTSW	6	119962955	missense	probably benign	0.12
IGL03052:Wnk1	UTSW	6	119944799	splice site	probably benign
PIT4480001:Wnk1	UTSW	6	119963367	nonsense	probably null
R0044:Wnk1	UTSW	6	120037149	missense	probably damaging	0.99
R0207:Wnk1	UTSW	6	119952733	missense	probably damaging	1.00
R0333:Wnk1	UTSW	6	119928163	intron	probably benign
R0453:Wnk1	UTSW	6	119963151	missense	probably damaging	0.99
R0457:Wnk1	UTSW	6	119969332	missense	probably damaging	1.00
R0501:Wnk1	UTSW	6	119962803	missense	probably damaging	1.00
R0525:Wnk1	UTSW	6	119926564	missense	probably damaging	1.00
R0526:Wnk1	UTSW	6	119951992	missense	probably damaging	0.99
R0606:Wnk1	UTSW	6	119926683	missense	probably damaging	1.00
R0658:Wnk1	UTSW	6	119948505	missense	probably damaging	0.98
R1148:Wnk1	UTSW	6	119952006	splice site	probably benign
R1188:Wnk1	UTSW	6	119948709	nonsense	probably null
R1245:Wnk1	UTSW	6	119948457	missense	probably benign	0.26
R1449:Wnk1	UTSW	6	119952818	missense	probably damaging	1.00
R1469:Wnk1	UTSW	6	119950684	splice site	probably benign
R1869:Wnk1	UTSW	6	119951089	missense	probably damaging	1.00
R1871:Wnk1	UTSW	6	119951089	missense	probably damaging	1.00
R1928:Wnk1	UTSW	6	119952923	missense	probably damaging	1.00
R1959:Wnk1	UTSW	6	119969247	missense	probably damaging	0.98
R1961:Wnk1	UTSW	6	119969247	missense	probably damaging	0.98
R1964:Wnk1	UTSW	6	119934382	missense	possibly damaging	0.86
R1983:Wnk1	UTSW	6	119937578	missense	probably damaging	1.00
R2062:Wnk1	UTSW	6	119928157	splice site	probably null
R2144:Wnk1	UTSW	6	119948988	unclassified	probably benign
R2186:Wnk1	UTSW	6	119948567	missense	probably benign	0.26
R2281:Wnk1	UTSW	6	119963640	splice site	probably null
R2338:Wnk1	UTSW	6	119969534	missense	probably benign	0.42
R2420:Wnk1	UTSW	6	119936367	critical splice donor site	probably null
R3727:Wnk1	UTSW	6	119992453	missense	probably damaging	1.00
R3773:Wnk1	UTSW	6	120002280	missense	possibly damaging	0.88
R3836:Wnk1	UTSW	6	119950043	missense	probably damaging	1.00
R3837:Wnk1	UTSW	6	119950043	missense	probably damaging	1.00
R3903:Wnk1	UTSW	6	119949051	missense	probably damaging	1.00
R4031:Wnk1	UTSW	6	119951068	missense	probably damaging	1.00
R4095:Wnk1	UTSW	6	119948126	missense	probably damaging	1.00
R4232:Wnk1	UTSW	6	119949261	missense	possibly damaging	0.90
R4422:Wnk1	UTSW	6	119953895	missense	probably benign	0.41
R4423:Wnk1	UTSW	6	119926426	missense	probably damaging	1.00
R4572:Wnk1	UTSW	6	119951911	missense	possibly damaging	0.49
R4704:Wnk1	UTSW	6	119965744	missense	possibly damaging	0.83
R4755:Wnk1	UTSW	6	119963470	missense	probably damaging	0.98
R4812:Wnk1	UTSW	6	119952771	missense	probably benign	0.16
R4822:Wnk1	UTSW	6	119962438	missense	probably benign	0.02
R4879:Wnk1	UTSW	6	119949377	missense	probably damaging	1.00
R4970:Wnk1	UTSW	6	119965735	intron	probably benign
R5002:Wnk1	UTSW	6	119937963	missense	probably benign	0.13
R5037:Wnk1	UTSW	6	119965735	intron	probably benign
R5152:Wnk1	UTSW	6	120002280	missense	possibly damaging	0.88
R5257:Wnk1	UTSW	6	120037188	missense	probably benign	0.00
R5354:Wnk1	UTSW	6	119968523	missense	probably benign	0.01
R5421:Wnk1	UTSW	6	119952818	missense	probably damaging	1.00
R5564:Wnk1	UTSW	6	119948891	unclassified	probably benign
R5600:Wnk1	UTSW	6	119949358	missense	probably damaging	1.00
R5847:Wnk1	UTSW	6	119992408	missense	probably damaging	1.00
R6083:Wnk1	UTSW	6	120037601	missense	probably damaging	0.99
R6110:Wnk1	UTSW	6	119972997	intron	probably benign
R6128:Wnk1	UTSW	6	119963786	splice site	probably null
R6237:Wnk1	UTSW	6	119952767	missense	probably damaging	1.00
R6341:Wnk1	UTSW	6	119948585	missense	probably damaging	1.00
R6467:Wnk1	UTSW	6	119962955	missense	probably benign	0.12
R6696:Wnk1	UTSW	6	119948282	missense	probably damaging	1.00
R6888:Wnk1	UTSW	6	119948781	missense	probably benign	0.26
R6923:Wnk1	UTSW	6	119965678	intron	probably benign
R7024:Wnk1	UTSW	6	119965726	intron	probably benign
R7072:Wnk1	UTSW	6	119937861	missense	unknown
R7087:Wnk1	UTSW	6	120037530	missense	possibly damaging	0.94
R7102:Wnk1	UTSW	6	119948307	missense	unknown
R7134:Wnk1	UTSW	6	119926428	missense	unknown
R7137:Wnk1	UTSW	6	120038212	unclassified	probably benign
R7142:Wnk1	UTSW	6	119949279	missense	probably benign	0.09
R7174:Wnk1	UTSW	6	119970978	missense	probably damaging	1.00
R7205:Wnk1	UTSW	6	119943878	splice site	probably null
R7218:Wnk1	UTSW	6	120002273	nonsense	probably null
R7498:Wnk1	UTSW	6	119927196	missense	unknown
R7599:Wnk1	UTSW	6	119929828	missense	possibly damaging	0.83
R7615:Wnk1	UTSW	6	119932738	missense	probably benign	0.27
R7799:Wnk1	UTSW	6	119949176	missense	probably benign	0.04
R7979:Wnk1	UTSW	6	120037448	missense	probably damaging	1.00
R8075:Wnk1	UTSW	6	119932714	missense	probably damaging	0.99
R8331:Wnk1	UTSW	6	119953833	missense	probably benign	0.09
R8343:Wnk1	UTSW	6	119963493	missense	probably damaging	0.99
R8348:Wnk1	UTSW	6	119929999	splice site	probably null
R8359:Wnk1	UTSW	6	119992447	missense	probably damaging	1.00
R8424:Wnk1	UTSW	6	119934427	missense	unknown
R8519:Wnk1	UTSW	6	119950083	missense	probably damaging	1.00
R8930:Wnk1	UTSW	6	119963265	missense	probably damaging	0.99
R8932:Wnk1	UTSW	6	119963265	missense	probably damaging	0.99
R8933:Wnk1	UTSW	6	120036998	missense	probably damaging	1.00
R9005:Wnk1	UTSW	6	119962432	missense	probably damaging	1.00
R9147:Wnk1	UTSW	6	119948670	missense	unknown
R9148:Wnk1	UTSW	6	119948670	missense	unknown
R9354:Wnk1	UTSW	6	119965699	missense	unknown
R9379:Wnk1	UTSW	6	119951717	missense	probably damaging	1.00
X0064:Wnk1	UTSW	6	120037032	missense	possibly damaging	0.93
Z1177:Wnk1	UTSW	6	119948205	missense	unknown

Predicted Primers

PCR Primer
(F):5'- GCTCAAAATGTCAAGATACTGGAAG -3'
(R):5'- GGTGATCACAAGACCATGGC -3'

Sequencing Primer
(F):5'- CCCTTCTATTAATCATGGGTACAAG -3'
(R):5'- CTAAAGCTATCAAAGATAGGGTGTCC -3'

Posted On

2015-04-06