Mutagenetix > Incidental Mutation

Incidental Mutation 'IGL02133:Wls'

281066

Institutional Source

Australian Phenomics Network (link to record)

Gene Symbol

Wls

Ensembl Gene

ENSMUSG00000028173

Gene Name

wntless WNT ligand secretion mediator

Synonyms

5031439A09Rik, Gpr177

Accession Numbers

Is this an essential gene?

Essential (E-score: 1.000) question?

Stock #

IGL02133

Quality Score

Status

Chromosome

Chromosomal Location

159839672-159938664 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 159897370 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 140 (Y140C)

Ref Sequence

ENSEMBL: ENSMUSP00000143475 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000068952] [ENSMUST00000198878] [ENSMUST00000200191]

AlphaFold

Q6DID7

Predicted Effect

probably damaging
Transcript: ENSMUST00000068952
AA Change: Y140C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000067898
Gene: ENSMUSG00000028173
AA Change: Y140C

Domain	Start	End	E-Value	Type
transmembrane domain	13	35	N/A	INTRINSIC
low complexity region	36	47	N/A	INTRINSIC
Pfam:MIG-14_Wnt-bd	178	496	3.7e-97	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000197328

Predicted Effect

probably damaging
Transcript: ENSMUST00000198878
AA Change: Y140C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000143475
Gene: ENSMUSG00000028173
AA Change: Y140C

Domain	Start	End	E-Value	Type
transmembrane domain	13	35	N/A	INTRINSIC
low complexity region	36	47	N/A	INTRINSIC
Pfam:MIG-14_Wnt-bd	177	497	2.8e-94	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000200191

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000200571

Coding Region Coverage

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for null alleles exhibit impaired body axis and triploblastic development dying prior to E10.5. Mice homozygous for a floxed allele activated in keratinocytes exhibit a psoriasiform dermatitis-like phenotype. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 33 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700011H14Rik	T	A	14: 49,232,967	Q165L	probably benign	Het
9530068E07Rik	G	T	11: 52,403,510	A193S	probably damaging	Het
Adam4	A	G	12: 81,420,029	V606A	probably benign	Het
Atp2c2	A	G	8: 119,754,335	I821V	probably benign	Het
Bmi1	A	T	2: 18,683,677	R160W	probably damaging	Het
Ccdc88c	G	A	12: 100,940,090	R1062C	probably damaging	Het
Cntnap3	A	T	13: 64,751,673		probably benign	Het
Col20a1	C	T	2: 181,007,144	T940I	probably damaging	Het
Csmd3	A	C	15: 47,857,942	M1535R	possibly damaging	Het
Dhx38	C	A	8: 109,558,241	E487*	probably null	Het
Fam186b	A	G	15: 99,273,703	S888P	probably damaging	Het
Fancm	G	A	12: 65,106,475	G1235D	probably benign	Het
Grin3a	C	T	4: 49,792,946	W262*	probably null	Het
Gtdc1	A	T	2: 44,575,443	M305K	probably damaging	Het
H2-M9	T	C	17: 36,641,737	E139G	possibly damaging	Het
Haus1	A	T	18: 77,766,911	L53Q	probably damaging	Het
Hgfac	A	G	5: 35,046,587	Y483C	probably damaging	Het
Hipk2	C	T	6: 38,819,134	V67I	probably benign	Het
Kbtbd8	T	C	6: 95,121,732		probably benign	Het
Kndc1	A	G	7: 139,920,767	T683A	probably benign	Het
Ldhb	A	T	6: 142,492,500	M277K	probably benign	Het
Mgam	T	C	6: 40,643,076	L33P	probably damaging	Het
Ndst1	A	G	18: 60,699,546	F571L	probably benign	Het
Neb	G	T	2: 52,212,804		probably null	Het
Nrxn1	A	G	17: 90,643,243	S503P	probably damaging	Het
Paqr3	A	G	5: 97,095,931	V308A	probably benign	Het
Scnn1g	T	C	7: 121,743,699	F292L	probably damaging	Het
Stk3	A	G	15: 35,099,516	F88S	probably damaging	Het
Tcaf2	T	A	6: 42,627,396	E683V	probably benign	Het
Tprkb	T	C	6: 85,927,911	V123A	probably benign	Het
Ush2a	T	C	1: 188,443,343	Y1213H	probably damaging	Het
Vwa5b1	A	G	4: 138,586,557		probably null	Het
Zan	A	G	5: 137,411,498	S28P	possibly damaging	Het

Other mutations in Wls

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01924:Wls	APN	3	159901443	nonsense	probably null
IGL02065:Wls	APN	3	159911356	missense	probably damaging	0.99
IGL02073:Wls	APN	3	159907253	critical splice donor site	probably null
R0374:Wls	UTSW	3	159897437	nonsense	probably null
R0561:Wls	UTSW	3	159873068	missense	probably benign	0.32
R1697:Wls	UTSW	3	159897358	missense	probably benign	0.12
R1791:Wls	UTSW	3	159911813	missense	probably benign	0.17
R2444:Wls	UTSW	3	159907230	missense	probably damaging	1.00
R3161:Wls	UTSW	3	159897436	missense	probably damaging	1.00
R4285:Wls	UTSW	3	159934266	missense	probably benign
R4468:Wls	UTSW	3	159872927	missense	probably damaging	0.96
R4472:Wls	UTSW	3	159897383	missense	probably benign	0.01
R4782:Wls	UTSW	3	159897445	missense	probably benign	0.03
R4799:Wls	UTSW	3	159897445	missense	probably benign	0.03
R4809:Wls	UTSW	3	159897445	missense	probably benign	0.03
R5006:Wls	UTSW	3	159911791	missense	possibly damaging	0.68
R5212:Wls	UTSW	3	159873008	missense	probably benign	0.15
R5434:Wls	UTSW	3	159934340	missense	probably damaging	0.97
R5694:Wls	UTSW	3	159839987	missense	probably benign	0.01
R6315:Wls	UTSW	3	159934371	critical splice donor site	probably null
R7069:Wls	UTSW	3	159934329	missense	probably damaging	1.00
R7243:Wls	UTSW	3	159909765	missense	possibly damaging	0.49
R7529:Wls	UTSW	3	159873007	missense	probably benign	0.43
R7697:Wls	UTSW	3	159911318	missense	probably benign	0.21
R7842:Wls	UTSW	3	159873179	missense	probably benign	0.09
R8136:Wls	UTSW	3	159873124	missense	probably damaging	1.00
R8536:Wls	UTSW	3	159873111	missense	probably damaging	0.96
R8816:Wls	UTSW	3	159934292	missense	possibly damaging	0.89
R9074:Wls	UTSW	3	159909766	missense	possibly damaging	0.86

Posted On

2015-04-16