Incidental Mutation 'IGL02183:Wif1'
ID284295
Institutional Source Australian Phenomics Network (link to record)
Gene Symbol Wif1
Ensembl Gene ENSMUSG00000020218
Gene NameWnt inhibitory factor 1
SynonymsWIF-1
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #IGL02183
Quality Score
Status
Chromosome10
Chromosomal Location121033960-121100650 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) C to T at 121075276 bp
ZygosityHeterozygous
Amino Acid Change Arginine to Cysteine at position 107 (R107C)
Ref Sequence ENSEMBL: ENSMUSP00000020439 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000020439] [ENSMUST00000175867]
Predicted Effect probably damaging
Transcript: ENSMUST00000020439
AA Change: R107C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000020439
Gene: ENSMUSG00000020218
AA Change: R107C

DomainStartEndE-ValueType
signal peptide 1 28 N/A INTRINSIC
WIF 35 179 8.47e-90 SMART
EGF 181 210 3.88e-3 SMART
EGF 213 242 4.46e-3 SMART
EGF 245 274 4.7e-2 SMART
EGF 277 306 1.69e-3 SMART
EGF 309 338 7.95e0 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000175867
AA Change: R107C

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000135486
Gene: ENSMUSG00000020218
AA Change: R107C

DomainStartEndE-ValueType
signal peptide 1 28 N/A INTRINSIC
WIF 35 179 8.47e-90 SMART
EGF 181 210 3.88e-3 SMART
EGF 213 242 4.46e-3 SMART
EGF 245 274 4.7e-2 SMART
EGF 295 324 7.95e0 SMART
Coding Region Coverage
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers. [provided by RefSeq, Jun 2010]
PHENOTYPE: Homozygous null mice are viable and fertile but display increased susceptibility to spontaneous and induced osteosarcomas. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 64 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2010300C02Rik G A 1: 37,625,378 P480S possibly damaging Het
Ace2 T A X: 164,177,469 probably benign Het
Acsm4 A T 7: 119,693,852 probably null Het
Apcdd1 T C 18: 62,951,854 M374T probably damaging Het
Arid4b A G 13: 14,169,990 E551G possibly damaging Het
Bag4 A T 8: 25,768,030 L423Q probably damaging Het
Celf1 C T 2: 91,001,486 P96S probably damaging Het
Cry2 G A 2: 92,413,039 R486W probably damaging Het
Csn1s1 G A 5: 87,677,618 S228N possibly damaging Het
Ctnnd2 A G 15: 31,020,740 Y1124C probably damaging Het
Cyp2j7 T C 4: 96,230,147 probably benign Het
Dock7 A T 4: 98,958,991 C1695S possibly damaging Het
Elmo3 T C 8: 105,308,323 L415P probably benign Het
Entpd5 A G 12: 84,380,380 probably benign Het
Gm7534 A G 4: 134,201,980 L338S probably benign Het
Gpatch11 T C 17: 78,842,231 probably null Het
Gprin3 C A 6: 59,353,162 R720I possibly damaging Het
Gria4 T G 9: 4,502,460 T358P probably damaging Het
Hcn2 T C 10: 79,724,813 probably null Het
Hivep3 A G 4: 120,132,024 T1891A probably benign Het
Hmgcr A G 13: 96,663,127 V153A probably damaging Het
Ifnar1 T C 16: 91,505,146 V503A possibly damaging Het
Igf2r G A 17: 12,698,516 probably benign Het
Ighg2b A G 12: 113,307,829 S35P unknown Het
Jmy T C 13: 93,499,242 D22G possibly damaging Het
Kdm5b T C 1: 134,624,931 I1215T probably benign Het
Krt84 T C 15: 101,532,356 I134V unknown Het
Magi3 T A 3: 104,085,347 M270L probably benign Het
Maneal G A 4: 124,860,416 T198I probably benign Het
Map7d3 A G X: 56,822,231 probably benign Het
Myh7 T A 14: 54,974,731 T1519S probably benign Het
Myo5a T C 9: 75,167,236 probably benign Het
Naip5 T C 13: 100,221,642 S1029G probably benign Het
Olfr1162 T C 2: 88,049,989 T212A possibly damaging Het
Olfr1205 A T 2: 88,832,028 I304F probably benign Het
Olfr125 G T 17: 37,835,413 C138F probably damaging Het
Olfr1279 T A 2: 111,306,418 V71E probably damaging Het
Olfr178 T A 16: 58,889,821 Q133L probably benign Het
Pald1 A T 10: 61,347,141 probably benign Het
Pan2 T A 10: 128,309,075 H230Q possibly damaging Het
Pcdh9 T C 14: 93,886,284 I817V probably benign Het
Piezo2 A G 18: 63,020,634 S2547P probably benign Het
Ppargc1b T A 18: 61,309,096 probably null Het
Prdm6 T C 18: 53,464,677 probably benign Het
Prr5l A T 2: 101,772,120 probably benign Het
Rab3gap2 T A 1: 185,271,468 L1020* probably null Het
Rhbdf1 T C 11: 32,210,543 H669R probably damaging Het
Rnf150 A T 8: 83,003,605 I255F probably damaging Het
Rnf17 T A 14: 56,507,868 D1360E probably null Het
Sag T C 1: 87,828,475 probably null Het
Scn2a C T 2: 65,671,603 T90I probably benign Het
Scn9a T A 2: 66,484,611 probably benign Het
Serpinb13 T C 1: 106,998,910 M212T probably damaging Het
Slc39a14 C T 14: 70,306,685 G484E possibly damaging Het
Slco1a1 A T 6: 141,921,943 probably benign Het
Slitrk3 A T 3: 73,049,979 Y487N probably damaging Het
Stkld1 T C 2: 26,946,659 M279T probably benign Het
Tmem161b T C 13: 84,272,254 Y125H probably damaging Het
Tmem71 A G 15: 66,555,025 probably benign Het
Ubxn7 T C 16: 32,369,383 F142L probably damaging Het
Vmn2r22 A G 6: 123,638,004 L209P probably damaging Het
Wdr73 A T 7: 80,893,760 W136R probably damaging Het
Ythdf2 G T 4: 132,205,574 L92M probably benign Het
Zfp53 T A 17: 21,500,250 I34N possibly damaging Het
Other mutations in Wif1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01384:Wif1 APN 10 121084950 missense possibly damaging 0.55
IGL01707:Wif1 APN 10 121083985 critical splice donor site probably null
IGL01932:Wif1 APN 10 121096035 missense probably damaging 1.00
R0653:Wif1 UTSW 10 121099799 missense probably benign 0.01
R1858:Wif1 UTSW 10 121083883 splice site probably null
R1869:Wif1 UTSW 10 121084919 missense probably benign 0.00
R1871:Wif1 UTSW 10 121084919 missense probably benign 0.00
R4056:Wif1 UTSW 10 121082194 missense probably benign 0.04
R4057:Wif1 UTSW 10 121082194 missense probably benign 0.04
R5056:Wif1 UTSW 10 121099779 missense probably benign 0.00
R6242:Wif1 UTSW 10 121034461 missense possibly damaging 0.82
R6504:Wif1 UTSW 10 121035091 missense probably damaging 0.96
R7220:Wif1 UTSW 10 121090114 missense possibly damaging 0.76
R7365:Wif1 UTSW 10 121083909 missense possibly damaging 0.94
R7456:Wif1 UTSW 10 121096649 missense probably benign
R7707:Wif1 UTSW 10 121083959 missense probably damaging 0.97
R8283:Wif1 UTSW 10 121096047 missense probably benign
Z1176:Wif1 UTSW 10 121096656 missense probably damaging 1.00
Posted On2015-04-16