Mutagenetix > Incidental Mutations

Incidental Mutation 'IGL02343:Cry2'

289171

Institutional Source

Australian Phenomics Network (link to record)

Gene Symbol

Cry2

Ensembl Gene

ENSMUSG00000068742

Gene Name

cryptochrome 2 (photolyase-like)

Synonyms

Accession Numbers

Is this an essential gene?

Probably essential (E-score: 0.752) question?

Stock #

IGL02343

Quality Score

Status

Chromosome

Chromosomal Location

92403646-92434043 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 92426921 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 101 (V101E)

Ref Sequence

ENSEMBL: ENSMUSP00000106909 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090559] [ENSMUST00000111278]

AlphaFold

Q9R194

Predicted Effect

possibly damaging
Transcript: ENSMUST00000090559
AA Change: V101E

PolyPhen 2 Score 0.899 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000088047
Gene: ENSMUSG00000068742
AA Change: V101E

Domain	Start	End	E-Value	Type
low complexity region	2	13	N/A	INTRINSIC
Pfam:DNA_photolyase	23	187	2.4e-50	PFAM
Pfam:FAD_binding_7	231	504	4.4e-89	PFAM
low complexity region	562	573	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000111278
AA Change: V101E

PolyPhen 2 Score 0.899 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000106909
Gene: ENSMUSG00000068742
AA Change: V101E

Domain	Start	End	E-Value	Type
low complexity region	2	13	N/A	INTRINSIC
Pfam:DNA_photolyase	23	189	3.6e-50	PFAM
Pfam:FAD_binding_7	230	506	1.4e-105	PFAM
low complexity region	562	573	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125488

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000126002

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000144842

Coding Region Coverage

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014]
PHENOTYPE: Homozygotes for targeted null mutations exhibit a one-hour longer circadian period under constant darkness, and reduced expression of another circadian gene in the suprachiasmatic nucleus in response to acute light exposure. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5830473C10Rik	C	A	5: 90,579,614	H364N	probably damaging	Het
Acbd5	T	A	2: 23,087,495	N168K	possibly damaging	Het
Aig1	T	C	10: 13,868,674	M29V	probably damaging	Het
Apba2	A	G	7: 64,695,146	E28G	probably damaging	Het
Bglap3	T	C	3: 88,369,116	N45S	possibly damaging	Het
Cables2	A	G	2: 180,260,279		probably null	Het
Cd109	A	G	9: 78,688,955		probably benign	Het
Cdk1	T	C	10: 69,340,501	N259D	probably benign	Het
Ces1h	A	G	8: 93,352,026	*563R	probably null	Het
Cfap46	C	T	7: 139,682,509	A115T	probably damaging	Het
Cyp4a31	A	G	4: 115,563,829	Y38C	probably damaging	Het
Dip2a	T	C	10: 76,319,478	Y130C	probably benign	Het
Eps8l1	A	G	7: 4,472,124	T366A	probably benign	Het
Esr2	C	T	12: 76,145,345	R303H	probably benign	Het
Fbxo41	T	G	6: 85,478,171	L617F	possibly damaging	Het
Fbxw15	A	T	9: 109,552,655		probably benign	Het
Lrriq1	T	A	10: 103,234,163		probably benign	Het
Me1	A	T	9: 86,654,641		probably null	Het
Moxd1	G	A	10: 24,279,864	V289I	probably damaging	Het
Muc19	T	G	15: 91,894,234		noncoding transcript	Het
Myo15b	A	G	11: 115,873,400		probably benign	Het
Nav1	A	T	1: 135,454,752	L1264*	probably null	Het
Nhlrc4	T	C	17: 25,943,361	*137W	probably null	Het
Nrxn3	A	G	12: 88,795,353	T57A	probably damaging	Het
Olfr1512	T	A	14: 52,372,477	D192V	probably damaging	Het
Orc2	A	T	1: 58,469,666		probably null	Het
Rpap2	G	A	5: 107,618,181		probably null	Het
S100a9	T	C	3: 90,695,224	H21R	probably damaging	Het
Spz1	T	A	13: 92,575,546	M141L	probably benign	Het
Stk40	A	G	4: 126,136,902	Y305C	probably damaging	Het
Stra6l	A	G	4: 45,869,588	Q218R	probably damaging	Het
T	C	A	17: 8,439,900		probably benign	Het
Tep1	T	C	14: 50,829,247	D2298G	probably damaging	Het
Trim40	A	T	17: 36,889,138	L16Q	probably benign	Het
Tubb4a	T	C	17: 57,081,538	I163V	probably benign	Het
Vwc2	T	A	11: 11,116,532	C207S	probably damaging	Het
Zfp407	A	G	18: 84,209,724	I1920T	possibly damaging	Het
Zfp429	T	C	13: 67,390,725	E200G	probably damaging	Het
Zfp451	C	T	1: 33,776,493	C792Y	probably damaging	Het

Other mutations in Cry2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01995:Cry2	APN	2	92424632	missense	probably benign	0.15
IGL02167:Cry2	APN	2	92433821	missense	possibly damaging	0.93
IGL02183:Cry2	APN	2	92413039	missense	probably damaging	0.99
IGL02432:Cry2	APN	2	92413667	missense	probably damaging	0.99
IGL02725:Cry2	APN	2	92413260	splice site	probably benign
IGL02932:Cry2	APN	2	92413117	nonsense	probably null
IGL03122:Cry2	APN	2	92413295	missense	probably damaging	1.00
IGL03366:Cry2	APN	2	92413715	missense	probably damaging	1.00
R0679:Cry2	UTSW	2	92413715	missense	probably damaging	1.00
R1325:Cry2	UTSW	2	92413770	missense	probably damaging	1.00
R1862:Cry2	UTSW	2	92424566	missense	probably damaging	1.00
R1891:Cry2	UTSW	2	92413640	missense	possibly damaging	0.93
R2189:Cry2	UTSW	2	92411692	missense	possibly damaging	0.84
R4032:Cry2	UTSW	2	92413827	missense	probably benign	0.00
R4689:Cry2	UTSW	2	92424554	missense	probably benign	0.38
R5130:Cry2	UTSW	2	92424599	missense	probably benign	0.28
R5145:Cry2	UTSW	2	92413060	missense	probably benign
R5970:Cry2	UTSW	2	92412967	missense	probably benign	0.08
R6179:Cry2	UTSW	2	92413842	missense	probably damaging	0.98
R7102:Cry2	UTSW	2	92413093	missense	probably damaging	0.99
R7158:Cry2	UTSW	2	92413715	missense	probably damaging	1.00
R7213:Cry2	UTSW	2	92413659	missense	probably benign	0.00
R7257:Cry2	UTSW	2	92412981	missense	possibly damaging	0.67
R7378:Cry2	UTSW	2	92413664	missense	probably damaging	1.00
R7427:Cry2	UTSW	2	92413047	missense	possibly damaging	0.74
R7428:Cry2	UTSW	2	92413047	missense	possibly damaging	0.74
R7440:Cry2	UTSW	2	92413638	missense	probably damaging	1.00
R7531:Cry2	UTSW	2	92413005	missense	probably damaging	0.98
R8234:Cry2	UTSW	2	92412629	missense	probably benign
R8350:Cry2	UTSW	2	92413941	missense	probably benign	0.00
R8450:Cry2	UTSW	2	92413941	missense	probably benign	0.00
R8496:Cry2	UTSW	2	92426939	missense	probably damaging	1.00
R9172:Cry2	UTSW	2	92413648	missense	probably damaging	1.00
R9283:Cry2	UTSW	2	92413904	missense	probably damaging	1.00

Posted On

2015-04-16