Mutagenetix > Incidental Mutation

Incidental Mutation 'R0357:Olfr424'

29851

Institutional Source

Beutler Lab

Gene Symbol

Olfr424

Ensembl Gene

ENSMUSG00000051528

Gene Name

olfactory receptor 424

Synonyms

MOR105-2, GA_x6K02T2P20D-21025190-21024243

MMRRC Submission

038563-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.240) question?

Stock #

R0357 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

174131517-174138257 bp(+) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 174137299 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Stop codon at position 185 (L185*)

Ref Sequence

ENSEMBL: ENSMUSP00000150840 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053941] [ENSMUST00000214751]

AlphaFold

E9Q0Q2

Predicted Effect

probably null
Transcript: ENSMUST00000053941
AA Change: L185*

SMART Domains

Protein: ENSMUSP00000054305
Gene: ENSMUSG00000051528
AA Change: L185*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	6.1e-63	PFAM
Pfam:7tm_1	41	289	1.8e-21	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000192471

Predicted Effect

probably null
Transcript: ENSMUST00000214751
AA Change: L185*

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000218625

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.0%
3x: 97.9%
10x: 95.3%
20x: 89.3%

Validation Efficiency

99% (74/75)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3110002H16Rik	T	C	18: 12,179,209	S169P	possibly damaging	Het
4930402H24Rik	A	T	2: 130,712,946		probably benign	Het
4931428F04Rik	A	T	8: 105,285,067	V222E	probably damaging	Het
4932431P20Rik	A	T	7: 29,535,582		noncoding transcript	Het
9230019H11Rik	A	T	10: 3,120,307		noncoding transcript	Het
9230019H11Rik	A	G	10: 3,125,788		noncoding transcript	Het
Abcf2	T	C	5: 24,573,465	K232E	probably benign	Het
AI837181	C	T	19: 5,426,703	T298I	possibly damaging	Het
Alox12	T	C	11: 70,242,536	Y614C	probably damaging	Het
Amn	A	T	12: 111,274,141		probably null	Het
Ankrd33b	A	G	15: 31,305,126	S121P	probably benign	Het
Aox1	A	G	1: 58,092,516	Y1028C	probably damaging	Het
Asph	C	A	4: 9,453,314	R736L	probably benign	Het
Atp2a3	G	A	11: 72,970,931		probably null	Het
Cables2	T	C	2: 180,262,232		probably benign	Het
Catsperg2	A	T	7: 29,714,901	Y360N	possibly damaging	Het
Ccdc129	T	A	6: 55,968,034	M580K	probably benign	Het
Cd163l1	T	G	7: 140,227,895	C660G	probably damaging	Het
Cdh4	T	C	2: 179,847,340	S282P	probably damaging	Het
Col5a3	C	T	9: 20,807,768		probably benign	Het
Ctso	A	T	3: 81,951,543		probably benign	Het
Cyp4f13	A	T	17: 32,932,651	Y125*	probably null	Het
Dapk1	T	A	13: 60,729,558	L537*	probably null	Het
Ddit4l	G	A	3: 137,626,185	R104Q	probably benign	Het
Def6	C	T	17: 28,223,935	H322Y	probably damaging	Het
Dnah6	T	C	6: 73,188,359	N588D	probably benign	Het
Dzip1	T	A	14: 118,909,538	I320F	probably damaging	Het
Epb41l5	T	C	1: 119,609,204	H319R	probably damaging	Het
Erc2	A	G	14: 27,777,022	E285G	probably damaging	Het
Fam109b	C	T	15: 82,343,316	A12V	probably damaging	Het
Fat4	G	A	3: 38,891,227	G1423E	probably damaging	Het
Foxp2	C	T	6: 15,409,840	P480S	probably damaging	Het
Gadd45gip1	G	A	8: 84,834,133	A126T	probably damaging	Het
Gbp5	G	A	3: 142,505,411	D301N	probably benign	Het
Gm10360	T	C	6: 70,424,313		noncoding transcript	Het
Gm6471	T	A	7: 142,833,867		noncoding transcript	Het
Gm8674	T	A	13: 49,902,113		noncoding transcript	Het
Hist2h2bb	A	C	3: 96,269,788	K13Q	probably null	Het
Ift172	A	G	5: 31,257,900	S1322P	possibly damaging	Het
Ift80	A	T	3: 68,914,653	Y686*	probably null	Het
Insrr	A	C	3: 87,808,646		probably null	Het
Krt83	C	T	15: 101,487,019	V399M	probably benign	Het
Macf1	T	C	4: 123,457,983	N3708S	probably damaging	Het
Mogat1	T	C	1: 78,512,040	S27P	probably benign	Het
Mrgpra4	A	T	7: 47,981,826	M9K	probably benign	Het
Mtus1	A	T	8: 41,083,526	S384R	possibly damaging	Het
Myo1a	T	A	10: 127,710,902	M306K	probably benign	Het
Noxa1	G	T	2: 25,085,850	D403E	probably damaging	Het
Ogdhl	T	C	14: 32,346,458	V884A	possibly damaging	Het
Olfr1335	A	C	4: 118,809,417	L149R	probably damaging	Het
Olfr1392	A	G	11: 49,293,786	N155S	probably damaging	Het
Olfr429	T	C	1: 174,089,109	V23A	possibly damaging	Het
Paxip1	G	A	5: 27,758,623		probably benign	Het
Paxx	T	A	2: 25,460,067	E145D	probably damaging	Het
Pde4d	T	C	13: 109,951,268	V560A	possibly damaging	Het
Plxnd1	C	T	6: 115,969,460	V847M	probably benign	Het
Polk	T	A	13: 96,504,597	M151L	probably damaging	Het
Ptprq	C	T	10: 107,686,199		probably benign	Het
Pum2	A	G	12: 8,721,785	Q371R	possibly damaging	Het
Reln	G	A	5: 21,950,822	A2224V	probably damaging	Het
Shroom1	A	G	11: 53,465,208	T362A	probably damaging	Het
Smarcd2	A	G	11: 106,267,332		probably null	Het
Spg11	A	C	2: 122,066,232		probably benign	Het
Tcaf3	T	A	6: 42,589,827	Y776F	probably damaging	Het
Thada	A	G	17: 84,230,936	V1548A	probably damaging	Het
Trpv2	C	T	11: 62,590,304	P410S	probably damaging	Het
Ube2u	G	T	4: 100,481,654	E39*	probably null	Het
Vmn2r2	C	T	3: 64,133,899		probably null	Het
Vmn2r24	TCC	TC	6: 123,815,410		probably null	Het
Zfp110	A	G	7: 12,836,375	Y43C	probably damaging	Het
Zfp605	A	G	5: 110,124,379	T55A	probably benign	Het

Other mutations in Olfr424

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01453:Olfr424	APN	1	174137113	missense	possibly damaging	0.46
IGL01651:Olfr424	APN	1	174137341	missense	probably damaging	0.96
R0194:Olfr424	UTSW	1	174136761	missense	probably benign	0.04
R0732:Olfr424	UTSW	1	174137415	missense	possibly damaging	0.85
R1103:Olfr424	UTSW	1	174136891	missense	probably benign	0.07
R1623:Olfr424	UTSW	1	174137317	missense	probably damaging	0.98
R1829:Olfr424	UTSW	1	174137194	missense	probably benign	0.12
R6617:Olfr424	UTSW	1	174137248	missense	probably damaging	1.00
R7060:Olfr424	UTSW	1	174136810	missense	probably benign	0.00
R7203:Olfr424	UTSW	1	174137114	nonsense	probably null
R7625:Olfr424	UTSW	1	174137167	missense	probably benign	0.13
R7994:Olfr424	UTSW	1	174136707	start gained	probably benign
R8035:Olfr424	UTSW	1	174136924	missense	probably damaging	1.00
R8127:Olfr424	UTSW	1	174137589	missense	probably damaging	1.00
R8802:Olfr424	UTSW	1	174137050	missense	probably damaging	1.00
R9102:Olfr424	UTSW	1	174136756	missense
R9296:Olfr424	UTSW	1	174137269	missense	probably benign	0.02
R9374:Olfr424	UTSW	1	174137319	missense	probably benign	0.34
R9551:Olfr424	UTSW	1	174137319	missense	probably benign	0.34
R9552:Olfr424	UTSW	1	174137319	missense	probably benign	0.34

Predicted Primers

PCR Primer
(F):5'- GATGGATACCCGTCTTCACAACCC -3'
(R):5'- GCAGCACAAGTAGAGAAAGCCTTCC -3'

Sequencing Primer
(F):5'- CCATTCACTTGGAAATTCAGAGGG -3'
(R):5'- CGACGGCCTTCACCAGAG -3'

Posted On

2013-04-24