Mutagenetix > Incidental Mutation

Incidental Mutation 'R0361:Ralgapa1'

30128

Institutional Source

Beutler Lab

Gene Symbol

Ralgapa1

Ensembl Gene

ENSMUSG00000021027

Gene Name

Ral GTPase activating protein, alpha subunit 1

Synonyms

Garnl1, 4930400K19Rik, 2310003F20Rik, Tulip1

MMRRC Submission

038567-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.755) question?

Stock #

R0361 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

55602896-55821167 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 55676569 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 1771 (I1771T)

Ref Sequence

ENSEMBL: ENSMUSP00000151857 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000085385] [ENSMUST00000110687] [ENSMUST00000219432] [ENSMUST00000220367] [ENSMUST00000226244]

AlphaFold

Q6GYP7

Predicted Effect

possibly damaging
Transcript: ENSMUST00000085385
AA Change: I1724T

PolyPhen 2 Score 0.887 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000082503
Gene: ENSMUSG00000021027
AA Change: I1724T

Domain	Start	End	E-Value	Type
low complexity region	644	651	N/A	INTRINSIC
low complexity region	676	690	N/A	INTRINSIC
low complexity region	692	704	N/A	INTRINSIC
low complexity region	894	915	N/A	INTRINSIC
low complexity region	1386	1395	N/A	INTRINSIC
low complexity region	1784	1798	N/A	INTRINSIC
Pfam:Rap_GAP	1824	2003	7.4e-66	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000110687
AA Change: I1724T

PolyPhen 2 Score 0.887 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000106315
Gene: ENSMUSG00000021027
AA Change: I1724T

Domain	Start	End	E-Value	Type
low complexity region	644	651	N/A	INTRINSIC
low complexity region	676	690	N/A	INTRINSIC
low complexity region	692	704	N/A	INTRINSIC
low complexity region	894	915	N/A	INTRINSIC
low complexity region	1386	1395	N/A	INTRINSIC
low complexity region	1784	1798	N/A	INTRINSIC
Pfam:Rap_GAP	1824	2001	1.9e-48	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000219432
AA Change: I1771T

PolyPhen 2 Score 0.933 (Sensitivity: 0.80; Specificity: 0.94)

Predicted Effect

probably benign
Transcript: ENSMUST00000219566

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000220017

Predicted Effect

possibly damaging
Transcript: ENSMUST00000220367
AA Change: I1724T

PolyPhen 2 Score 0.881 (Sensitivity: 0.82; Specificity: 0.94)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000226244
AA Change: I2180T

PolyPhen 2 Score 0.462 (Sensitivity: 0.89; Specificity: 0.90)

Coding Region Coverage

1x: 99.1%
3x: 98.2%
10x: 96.0%
20x: 91.7%

Validation Efficiency

100% (1/1)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a major subunit of the RAL-GTPase activating protein. A similar protein in mouse binds E12, a transcriptional regulator of immunoglobulin genes. The mouse protein also functions in skeletal muscle by binding to the regulatory 14-3-3 proteins upon stimulation with insulin or muscle contraction. A pseudogene of this gene has been identified on chromosome 9. [provided by RefSeq, Oct 2016]

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700006A11Rik	T	G	3: 124,413,634 (GRCm38)	T303P	possibly damaging	Het
1700010I14Rik	G	T	17: 8,992,546 (GRCm38)	V176L	probably benign	Het
1700034J05Rik	T	C	6: 146,952,371 (GRCm38)	T262A	possibly damaging	Het
Adgrl3	A	T	5: 81,760,697 (GRCm38)	I1165F	probably damaging	Het
Ankhd1	T	C	18: 36,647,214 (GRCm38)	I1773T	probably damaging	Het
Api5	A	T	2: 94,423,497 (GRCm38)	L287*	probably null	Het
Apol10b	A	T	15: 77,585,386 (GRCm38)	M197K	possibly damaging	Het
Bcl2	G	A	1: 106,712,694 (GRCm38)	R63W	probably damaging	Het
Cacna1h	A	G	17: 25,389,422 (GRCm38)	M731T	probably damaging	Het
Cav1	C	A	6: 17,339,353 (GRCm38)	R146S	possibly damaging	Het
Cdhr2	A	T	13: 54,734,007 (GRCm38)	I1118F	probably damaging	Het
Cdk7	A	T	13: 100,711,554 (GRCm38)	Y153*	probably null	Het
Cemip	A	G	7: 83,964,010 (GRCm38)	I660T	probably benign	Het
Cfap65	A	T	1: 74,925,440 (GRCm38)	L518Q	probably damaging	Het
Cngb3	A	G	4: 19,366,467 (GRCm38)	H176R	probably benign	Het
Cux1	T	A	5: 136,279,497 (GRCm38)	I1263F	probably damaging	Het
Dnajc13	A	G	9: 104,167,059 (GRCm38)	M1867T	probably benign	Het
Dock2	A	G	11: 34,438,327 (GRCm38)	L202P	probably damaging	Het
Dyrk3	A	G	1: 131,130,032 (GRCm38)	S100P	probably benign	Het
Efr3b	A	T	12: 3,977,923 (GRCm38)	S376T	probably benign	Het
Eps8l2	A	C	7: 141,356,199 (GRCm38)	N222T	probably benign	Het
Ermp1	A	T	19: 29,631,406 (GRCm38)	Y158N	probably damaging	Het
Fam13a	A	G	6: 58,987,174 (GRCm38)	V91A	probably benign	Het
Fat3	A	G	9: 15,998,403 (GRCm38)	V2101A	possibly damaging	Het
Fsip2	T	C	2: 82,975,505 (GRCm38)	S723P	possibly damaging	Het
Garem1	G	T	18: 21,299,744 (GRCm38)	C9*	probably null	Het
Gdpd5	A	G	7: 99,458,790 (GRCm38)	I530V	possibly damaging	Het
Gm15217	T	A	14: 46,380,384 (GRCm38)		probably benign	Het
Gm4922	T	C	10: 18,783,541 (GRCm38)	T478A	probably benign	Het
Gm5483	T	C	16: 36,184,278 (GRCm38)	S7P	probably damaging	Het
H2-M5	A	G	17: 36,987,436 (GRCm38)	I329T	possibly damaging	Het
Ing4	G	A	6: 125,047,894 (GRCm38)	C200Y	probably damaging	Het
Kcnip1	A	T	11: 33,843,177 (GRCm38)	M5K	probably benign	Het
Kdsr	T	C	1: 106,747,787 (GRCm38)	E102G	probably damaging	Het
Krt15	C	T	11: 100,133,181 (GRCm38)	V346M	probably benign	Het
Lgr6	C	T	1: 134,994,010 (GRCm38)	A199T	probably damaging	Het
Lrrc55	A	T	2: 85,196,245 (GRCm38)	M145K	probably damaging	Het
Lrrtm2	A	G	18: 35,212,932 (GRCm38)	I439T	probably benign	Het
Map2k6	T	C	11: 110,499,509 (GRCm38)	F290L	probably damaging	Het
Mb21d1	T	A	9: 78,433,252 (GRCm38)	K399N	probably damaging	Het
Me1	T	A	9: 86,651,002 (GRCm38)	I136F	probably damaging	Het
Mfap2	A	G	4: 141,014,983 (GRCm38)	D98G	probably damaging	Het
Mfsd13a	C	T	19: 46,366,504 (GRCm38)	T40I	probably benign	Het
Mst1	T	C	9: 108,084,897 (GRCm38)	F696L	probably damaging	Het
Mta1	A	G	12: 113,133,341 (GRCm38)		probably null	Het
Myh15	A	T	16: 49,114,005 (GRCm38)	N645I	probably benign	Het
Myo7b	T	A	18: 32,014,209 (GRCm38)	I94F	probably damaging	Het
Nefh	A	T	11: 4,940,799 (GRCm38)	S607T	probably benign	Het
Noa1	G	A	5: 77,297,173 (GRCm38)	Q600*	probably null	Het
Nr2f2	A	G	7: 70,358,062 (GRCm38)	V71A	possibly damaging	Het
Oas2	A	T	5: 120,738,401 (GRCm38)	F492L	probably damaging	Het
Olfm3	T	A	3: 115,120,973 (GRCm38)	D211E	probably damaging	Het
Olfr1390	A	T	11: 49,340,814 (GRCm38)	Y94F	probably benign	Het
Osmr	A	G	15: 6,841,951 (GRCm38)		probably null	Het
Plagl2	A	T	2: 153,231,603 (GRCm38)	D459E	probably benign	Het
Plch2	T	C	4: 155,006,711 (GRCm38)	D148G	possibly damaging	Het
Plxnc1	C	A	10: 94,865,007 (GRCm38)	C605F	probably damaging	Het
Ppm1m	T	C	9: 106,198,126 (GRCm38)	E108G	probably damaging	Het
Prr14l	A	C	5: 32,793,641 (GRCm38)	L1936R	probably damaging	Het
Rhobtb2	T	C	14: 69,795,908 (GRCm38)	T538A	probably benign	Het
Rictor	A	G	15: 6,784,107 (GRCm38)	N1025D	possibly damaging	Het
Sec23a	T	G	12: 58,991,018 (GRCm38)	D324A	probably damaging	Het
Srgap1	A	T	10: 122,047,192 (GRCm38)	M1K	probably null	Het
Syne2	T	A	12: 75,918,610 (GRCm38)	F801I	probably benign	Het
Synrg	T	A	11: 84,024,337 (GRCm38)		probably null	Het
Tas2r137	T	G	6: 40,491,298 (GRCm38)	F21V	probably benign	Het
Tmem260	A	T	14: 48,452,047 (GRCm38)	T108S	possibly damaging	Het
Trim2	T	C	3: 84,190,776 (GRCm38)	Y406C	probably damaging	Het
Ttn	T	C	2: 76,843,402 (GRCm38)		probably benign	Het
Vmn1r53	A	T	6: 90,224,082 (GRCm38)	S87T	possibly damaging	Het
Vmn2r115	T	A	17: 23,345,222 (GRCm38)	Y123N	probably benign	Het
Vmn2r28	T	A	7: 5,493,716 (GRCm38)	I46F	probably benign	Het
Zan	T	C	5: 137,396,766 (GRCm38)	T4381A	unknown	Het
Zfp457	A	G	13: 67,292,646 (GRCm38)	F622L	probably damaging	Het
Zfp994	A	T	17: 22,200,110 (GRCm38)	N619K	probably benign	Het
Zfy1	T	C	Y: 726,121 (GRCm38)	H548R	possibly damaging	Het
Zmym4	A	T	4: 126,911,145 (GRCm38)	S441T	probably benign	Het

Other mutations in Ralgapa1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Ralgapa1	APN	12	55,722,773 (GRCm38)	missense	probably damaging	0.98
IGL00494:Ralgapa1	APN	12	55,747,185 (GRCm38)	missense	probably damaging	1.00
IGL00731:Ralgapa1	APN	12	55,702,452 (GRCm38)	missense	possibly damaging	0.94
IGL00851:Ralgapa1	APN	12	55,709,575 (GRCm38)	missense	possibly damaging	0.93
IGL01133:Ralgapa1	APN	12	55,642,359 (GRCm38)	missense	probably damaging	0.99
IGL01133:Ralgapa1	APN	12	55,642,348 (GRCm38)	missense	probably damaging	1.00
IGL01354:Ralgapa1	APN	12	55,777,316 (GRCm38)	missense	possibly damaging	0.68
IGL01514:Ralgapa1	APN	12	55,719,657 (GRCm38)	missense	probably damaging	0.97
IGL02033:Ralgapa1	APN	12	55,642,477 (GRCm38)	missense	possibly damaging	0.69
IGL02064:Ralgapa1	APN	12	55,708,077 (GRCm38)	missense	probably damaging	1.00
IGL02556:Ralgapa1	APN	12	55,642,449 (GRCm38)	missense	possibly damaging	0.80
IGL02605:Ralgapa1	APN	12	55,712,665 (GRCm38)	missense	possibly damaging	0.90
IGL02657:Ralgapa1	APN	12	55,673,507 (GRCm38)	missense	probably damaging	1.00
IGL02676:Ralgapa1	APN	12	55,676,417 (GRCm38)	missense	probably damaging	1.00
IGL02894:Ralgapa1	APN	12	55,717,069 (GRCm38)	missense	possibly damaging	0.79
IGL02944:Ralgapa1	APN	12	55,757,951 (GRCm38)	missense	probably benign	0.01
Anhydrous	UTSW	12	55,795,778 (GRCm38)	critical splice acceptor site	probably null
Aqueous	UTSW	12	55,698,854 (GRCm38)	missense	probably damaging	1.00
bantam	UTSW	12	55,722,773 (GRCm38)	critical splice donor site	probably null
Deliquescent	UTSW	12	55,782,900 (GRCm38)	splice site	probably benign
wickedwarlock	UTSW	12	55,777,292 (GRCm38)	missense	probably null	0.99
F5770:Ralgapa1	UTSW	12	55,795,653 (GRCm38)	splice site	probably benign
IGL03046:Ralgapa1	UTSW	12	55,695,157 (GRCm38)	missense	probably damaging	1.00
R0011:Ralgapa1	UTSW	12	55,786,263 (GRCm38)	missense	probably damaging	0.99
R0096:Ralgapa1	UTSW	12	55,739,505 (GRCm38)	missense	probably damaging	1.00
R0277:Ralgapa1	UTSW	12	55,677,238 (GRCm38)	missense	probably damaging	0.99
R0323:Ralgapa1	UTSW	12	55,677,238 (GRCm38)	missense	probably damaging	0.99
R0333:Ralgapa1	UTSW	12	55,782,900 (GRCm38)	splice site	probably benign
R0385:Ralgapa1	UTSW	12	55,677,038 (GRCm38)	missense	probably damaging	1.00
R0386:Ralgapa1	UTSW	12	55,708,067 (GRCm38)	missense	probably benign	0.03
R0498:Ralgapa1	UTSW	12	55,689,791 (GRCm38)	missense	possibly damaging	0.66
R0552:Ralgapa1	UTSW	12	55,676,765 (GRCm38)	missense	probably benign	0.27
R0564:Ralgapa1	UTSW	12	55,782,885 (GRCm38)	missense	possibly damaging	0.84
R0611:Ralgapa1	UTSW	12	55,795,698 (GRCm38)	missense	probably damaging	0.99
R0730:Ralgapa1	UTSW	12	55,665,663 (GRCm38)	missense	probably damaging	1.00
R0741:Ralgapa1	UTSW	12	55,676,581 (GRCm38)	missense	probably damaging	0.99
R0815:Ralgapa1	UTSW	12	55,782,777 (GRCm38)	splice site	probably benign
R0815:Ralgapa1	UTSW	12	55,762,681 (GRCm38)	nonsense	probably null
R0863:Ralgapa1	UTSW	12	55,782,777 (GRCm38)	splice site	probably benign
R0863:Ralgapa1	UTSW	12	55,762,681 (GRCm38)	nonsense	probably null
R1068:Ralgapa1	UTSW	12	55,790,310 (GRCm38)	critical splice donor site	probably null
R1147:Ralgapa1	UTSW	12	55,702,480 (GRCm38)	missense	probably damaging	1.00
R1147:Ralgapa1	UTSW	12	55,702,480 (GRCm38)	missense	probably damaging	1.00
R1256:Ralgapa1	UTSW	12	55,762,661 (GRCm38)	missense	possibly damaging	0.94
R1343:Ralgapa1	UTSW	12	55,707,978 (GRCm38)	missense	probably damaging	1.00
R1378:Ralgapa1	UTSW	12	55,676,926 (GRCm38)	missense	probably damaging	1.00
R1474:Ralgapa1	UTSW	12	55,741,480 (GRCm38)	missense	probably benign	0.09
R1494:Ralgapa1	UTSW	12	55,684,524 (GRCm38)	missense	probably damaging	0.99
R1593:Ralgapa1	UTSW	12	55,770,703 (GRCm38)	missense	probably damaging	1.00
R1607:Ralgapa1	UTSW	12	55,741,536 (GRCm38)	missense	probably damaging	1.00
R1681:Ralgapa1	UTSW	12	55,762,603 (GRCm38)	missense	probably benign	0.35
R1689:Ralgapa1	UTSW	12	55,676,767 (GRCm38)	missense	possibly damaging	0.79
R1714:Ralgapa1	UTSW	12	55,642,389 (GRCm38)	missense	probably damaging	1.00
R1832:Ralgapa1	UTSW	12	55,757,967 (GRCm38)	missense	probably benign	0.03
R1870:Ralgapa1	UTSW	12	55,677,032 (GRCm38)	missense	possibly damaging	0.66
R2040:Ralgapa1	UTSW	12	55,786,322 (GRCm38)	missense	probably damaging	1.00
R2043:Ralgapa1	UTSW	12	55,677,026 (GRCm38)	missense	probably damaging	0.99
R2046:Ralgapa1	UTSW	12	55,695,160 (GRCm38)	missense	probably damaging	1.00
R2109:Ralgapa1	UTSW	12	55,776,188 (GRCm38)	missense	possibly damaging	0.90
R2114:Ralgapa1	UTSW	12	55,786,349 (GRCm38)	critical splice acceptor site	probably null
R2115:Ralgapa1	UTSW	12	55,786,349 (GRCm38)	critical splice acceptor site	probably null
R2202:Ralgapa1	UTSW	12	55,612,800 (GRCm38)	splice site	probably null
R2203:Ralgapa1	UTSW	12	55,612,800 (GRCm38)	splice site	probably null
R2233:Ralgapa1	UTSW	12	55,717,071 (GRCm38)	missense	probably benign	0.13
R2235:Ralgapa1	UTSW	12	55,717,071 (GRCm38)	missense	probably benign	0.13
R2341:Ralgapa1	UTSW	12	55,677,124 (GRCm38)	missense	possibly damaging	0.66
R2507:Ralgapa1	UTSW	12	55,718,201 (GRCm38)	missense	probably damaging	1.00
R2508:Ralgapa1	UTSW	12	55,718,201 (GRCm38)	missense	probably damaging	1.00
R2972:Ralgapa1	UTSW	12	55,820,755 (GRCm38)	missense	possibly damaging	0.61
R3160:Ralgapa1	UTSW	12	55,709,586 (GRCm38)	missense	probably damaging	1.00
R3162:Ralgapa1	UTSW	12	55,709,586 (GRCm38)	missense	probably damaging	1.00
R3401:Ralgapa1	UTSW	12	55,659,137 (GRCm38)	missense	possibly damaging	0.66
R3416:Ralgapa1	UTSW	12	55,770,613 (GRCm38)	splice site	probably benign
R3499:Ralgapa1	UTSW	12	55,695,143 (GRCm38)	splice site	probably benign
R3799:Ralgapa1	UTSW	12	55,659,130 (GRCm38)	missense	probably damaging	1.00
R3948:Ralgapa1	UTSW	12	55,698,767 (GRCm38)	missense	probably damaging	1.00
R4039:Ralgapa1	UTSW	12	55,795,701 (GRCm38)	missense	probably damaging	0.99
R4120:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4165:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4166:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4212:Ralgapa1	UTSW	12	55,739,330 (GRCm38)	critical splice donor site	probably null
R4232:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4233:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4234:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4235:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4399:Ralgapa1	UTSW	12	55,795,778 (GRCm38)	critical splice acceptor site	probably null
R4698:Ralgapa1	UTSW	12	55,677,276 (GRCm38)	splice site	probably null
R4715:Ralgapa1	UTSW	12	55,693,458 (GRCm38)	missense	probably damaging	1.00
R4755:Ralgapa1	UTSW	12	55,712,748 (GRCm38)	missense	probably damaging	1.00
R4810:Ralgapa1	UTSW	12	55,794,993 (GRCm38)	critical splice donor site	probably null
R4827:Ralgapa1	UTSW	12	55,676,437 (GRCm38)	missense	probably damaging	1.00
R4849:Ralgapa1	UTSW	12	55,698,803 (GRCm38)	missense	probably damaging	0.99
R4934:Ralgapa1	UTSW	12	55,762,574 (GRCm38)	missense	possibly damaging	0.94
R5006:Ralgapa1	UTSW	12	55,718,114 (GRCm38)	missense	probably benign	0.02
R5114:Ralgapa1	UTSW	12	55,612,723 (GRCm38)	missense	possibly damaging	0.84
R5140:Ralgapa1	UTSW	12	55,665,674 (GRCm38)	missense	probably damaging	1.00
R5140:Ralgapa1	UTSW	12	55,776,152 (GRCm38)	missense	probably damaging	1.00
R5168:Ralgapa1	UTSW	12	55,758,032 (GRCm38)	missense	probably benign	0.05
R5407:Ralgapa1	UTSW	12	55,676,797 (GRCm38)	missense	possibly damaging	0.93
R5441:Ralgapa1	UTSW	12	55,719,623 (GRCm38)	missense	probably damaging	1.00
R5473:Ralgapa1	UTSW	12	55,676,710 (GRCm38)	missense	probably benign	0.41
R5624:Ralgapa1	UTSW	12	55,612,738 (GRCm38)	missense	probably damaging	1.00
R5766:Ralgapa1	UTSW	12	55,820,766 (GRCm38)	start codon destroyed	probably null	0.99
R5826:Ralgapa1	UTSW	12	55,677,113 (GRCm38)	missense	probably damaging	1.00
R5950:Ralgapa1	UTSW	12	55,738,265 (GRCm38)	missense	possibly damaging	0.58
R5980:Ralgapa1	UTSW	12	55,770,616 (GRCm38)	splice site	probably null
R6019:Ralgapa1	UTSW	12	55,684,042 (GRCm38)	missense	possibly damaging	0.92
R6065:Ralgapa1	UTSW	12	55,757,924 (GRCm38)	critical splice donor site	probably null
R6326:Ralgapa1	UTSW	12	55,747,146 (GRCm38)	missense	probably damaging	1.00
R6355:Ralgapa1	UTSW	12	55,698,854 (GRCm38)	missense	probably damaging	1.00
R6408:Ralgapa1	UTSW	12	55,683,910 (GRCm38)	nonsense	probably null
R6448:Ralgapa1	UTSW	12	55,719,661 (GRCm38)	missense	probably benign	0.14
R6453:Ralgapa1	UTSW	12	55,738,319 (GRCm38)	missense	probably damaging	1.00
R6590:Ralgapa1	UTSW	12	55,722,773 (GRCm38)	critical splice donor site	probably null
R6690:Ralgapa1	UTSW	12	55,722,773 (GRCm38)	critical splice donor site	probably null
R6738:Ralgapa1	UTSW	12	55,762,727 (GRCm38)	missense	probably damaging	1.00
R6836:Ralgapa1	UTSW	12	55,604,273 (GRCm38)	splice site	probably null
R6936:Ralgapa1	UTSW	12	55,786,212 (GRCm38)	missense	probably damaging	0.99
R6945:Ralgapa1	UTSW	12	55,776,191 (GRCm38)	missense	possibly damaging	0.64
R7028:Ralgapa1	UTSW	12	55,758,059 (GRCm38)	missense	probably damaging	1.00
R7075:Ralgapa1	UTSW	12	55,820,723 (GRCm38)	missense	possibly damaging	0.66
R7076:Ralgapa1	UTSW	12	55,721,576 (GRCm38)	missense	possibly damaging	0.82
R7098:Ralgapa1	UTSW	12	55,790,310 (GRCm38)	critical splice donor site	probably null
R7231:Ralgapa1	UTSW	12	55,604,191 (GRCm38)	missense	probably damaging	1.00
R7254:Ralgapa1	UTSW	12	55,695,193 (GRCm38)	missense	probably damaging	1.00
R7326:Ralgapa1	UTSW	12	55,709,004 (GRCm38)	missense	probably damaging	1.00
R7485:Ralgapa1	UTSW	12	55,712,672 (GRCm38)	missense	probably damaging	1.00
R7580:Ralgapa1	UTSW	12	55,718,228 (GRCm38)	missense	probably benign	0.00
R7677:Ralgapa1	UTSW	12	55,659,143 (GRCm38)	missense	probably damaging	0.96
R7702:Ralgapa1	UTSW	12	55,709,556 (GRCm38)	missense	probably damaging	1.00
R7702:Ralgapa1	UTSW	12	55,709,555 (GRCm38)	missense	probably damaging	1.00
R7707:Ralgapa1	UTSW	12	55,777,292 (GRCm38)	missense	probably null	0.99
R7723:Ralgapa1	UTSW	12	55,741,513 (GRCm38)	missense	probably benign
R7763:Ralgapa1	UTSW	12	55,757,955 (GRCm38)	missense	probably benign	0.28
R7791:Ralgapa1	UTSW	12	55,741,519 (GRCm38)	missense	probably damaging	0.97
R7812:Ralgapa1	UTSW	12	55,719,628 (GRCm38)	missense	possibly damaging	0.67
R7868:Ralgapa1	UTSW	12	55,612,638 (GRCm38)	missense	probably benign	0.00
R7895:Ralgapa1	UTSW	12	55,747,149 (GRCm38)	missense	probably benign	0.44
R7896:Ralgapa1	UTSW	12	55,697,878 (GRCm38)	missense	probably benign	0.01
R8004:Ralgapa1	UTSW	12	55,702,457 (GRCm38)	missense	probably damaging	0.99
R8094:Ralgapa1	UTSW	12	55,782,846 (GRCm38)	missense	probably damaging	0.99
R8213:Ralgapa1	UTSW	12	55,722,914 (GRCm38)	missense	probably damaging	0.99
R8307:Ralgapa1	UTSW	12	55,741,523 (GRCm38)	missense	probably damaging	0.99
R8423:Ralgapa1	UTSW	12	55,659,062 (GRCm38)	missense	probably damaging	0.99
R8462:Ralgapa1	UTSW	12	55,676,518 (GRCm38)	missense	possibly damaging	0.90
R8469:Ralgapa1	UTSW	12	55,739,413 (GRCm38)	missense	probably damaging	1.00
R8675:Ralgapa1	UTSW	12	55,738,217 (GRCm38)	missense	possibly damaging	0.93
R8802:Ralgapa1	UTSW	12	55,738,316 (GRCm38)	missense	probably damaging	0.99
R8937:Ralgapa1	UTSW	12	55,702,560 (GRCm38)	missense	probably damaging	0.96
R8953:Ralgapa1	UTSW	12	55,820,761 (GRCm38)	missense	probably damaging	0.99
R8974:Ralgapa1	UTSW	12	55,677,006 (GRCm38)	missense	probably benign
R9011:Ralgapa1	UTSW	12	55,605,529 (GRCm38)	intron	probably benign
R9089:Ralgapa1	UTSW	12	55,676,566 (GRCm38)	missense	probably damaging	0.97
R9124:Ralgapa1	UTSW	12	55,735,096 (GRCm38)	missense	probably damaging	1.00
R9254:Ralgapa1	UTSW	12	55,722,798 (GRCm38)	missense	probably damaging	1.00
R9320:Ralgapa1	UTSW	12	55,709,058 (GRCm38)	missense	possibly damaging	0.59
R9379:Ralgapa1	UTSW	12	55,722,798 (GRCm38)	missense	probably damaging	1.00
R9446:Ralgapa1	UTSW	12	55,708,023 (GRCm38)	missense	probably damaging	0.97
R9684:Ralgapa1	UTSW	12	55,612,700 (GRCm38)	missense	possibly damaging	0.63
Z1176:Ralgapa1	UTSW	12	55,709,080 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CGTTTGTCCCAGGAATTCATTCCCA -3'
(R):5'- CGGAAGAGCCTCCTACATCTAACGA -3'

Sequencing Primer
(F):5'- GTCCCAGGAATTCATTCCCAATATAC -3'
(R):5'- CGCAGTAAAAGATGGGCTTTCTC -3'

Posted On

2013-04-24