Mutagenetix > Incidental Mutation

Incidental Mutation 'R0361:Mta1'

30131

Institutional Source

Beutler Lab

Gene Symbol

Mta1

Ensembl Gene

ENSMUSG00000021144

Gene Name

metastasis associated 1

Synonyms

MMRRC Submission

038567-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0361 (G1)

Quality Score

218

Status

Not validated

Chromosome

Chromosomal Location

113061898-113100826 bp(+) (GRCm39)

Type of Mutation

splice site (4 bp from exon)

DNA Base Change (assembly)

A to G at 113096961 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000105349 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000009099] [ENSMUST00000069690] [ENSMUST00000109723] [ENSMUST00000109726] [ENSMUST00000109727]

AlphaFold

Q8K4B0

Predicted Effect

probably null
Transcript: ENSMUST00000009099

SMART Domains

Protein: ENSMUSP00000009099
Gene: ENSMUSG00000021144

Domain	Start	End	E-Value	Type
BAH	4	164	1.85e-30	SMART
ELM2	167	221	2.36e-13	SMART
SANT	284	333	2.62e-8	SMART
ZnF_GATA	387	441	2.6e-16	SMART
low complexity region	545	565	N/A	INTRINSIC
low complexity region	695	705	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000069690

SMART Domains

Protein: ENSMUSP00000064338
Gene: ENSMUSG00000021144

Domain	Start	End	E-Value	Type
BAH	4	147	2.7e-32	SMART
ELM2	150	204	2.36e-13	SMART
SANT	267	316	2.62e-8	SMART
ZnF_GATA	370	424	2.6e-16	SMART
low complexity region	528	548	N/A	INTRINSIC
low complexity region	678	688	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000109723

SMART Domains

Protein: ENSMUSP00000105345
Gene: ENSMUSG00000021144

Domain	Start	End	E-Value	Type
BAH	4	164	1.85e-30	SMART
ELM2	167	221	2.36e-13	SMART
SANT	284	333	2.62e-8	SMART
ZnF_GATA	387	441	2.6e-16	SMART
low complexity region	545	565	N/A	INTRINSIC
low complexity region	683	693	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000109726

SMART Domains

Protein: ENSMUSP00000105348
Gene: ENSMUSG00000021144

Domain	Start	End	E-Value	Type
BAH	4	147	2.7e-32	SMART
ELM2	150	204	2.36e-13	SMART
SANT	267	316	2.62e-8	SMART
ZnF_GATA	370	424	2.6e-16	SMART
low complexity region	528	548	N/A	INTRINSIC
low complexity region	678	688	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000109727

SMART Domains

Protein: ENSMUSP00000105349
Gene: ENSMUSG00000021144

Domain	Start	End	E-Value	Type
BAH	4	164	1.85e-30	SMART
ELM2	167	221	2.36e-13	SMART
SANT	284	333	2.62e-8	SMART
ZnF_GATA	387	441	2.6e-16	SMART
low complexity region	545	565	N/A	INTRINSIC
low complexity region	683	693	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130926

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000134488

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000156030

Coding Region Coverage

1x: 99.1%
3x: 98.2%
10x: 96.0%
20x: 91.7%

Validation Efficiency

100% (1/1)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased cellular sensitivity to ionizing radiation and increased retinal cell proliferation at E14.5. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700006A11Rik	T	G	3: 124,207,283 (GRCm39)	T303P	possibly damaging	Het
1700010I14Rik	G	T	17: 9,211,378 (GRCm39)	V176L	probably benign	Het
1700034J05Rik	T	C	6: 146,853,869 (GRCm39)	T262A	possibly damaging	Het
Adgrl3	A	T	5: 81,908,544 (GRCm39)	I1165F	probably damaging	Het
Ankhd1	T	C	18: 36,780,267 (GRCm39)	I1773T	probably damaging	Het
Api5	A	T	2: 94,253,842 (GRCm39)	L287*	probably null	Het
Apol10b	A	T	15: 77,469,586 (GRCm39)	M197K	possibly damaging	Het
Bcl2	G	A	1: 106,640,424 (GRCm39)	R63W	probably damaging	Het
Cacna1h	A	G	17: 25,608,396 (GRCm39)	M731T	probably damaging	Het
Cav1	C	A	6: 17,339,352 (GRCm39)	R146S	possibly damaging	Het
Cdhr2	A	T	13: 54,881,820 (GRCm39)	I1118F	probably damaging	Het
Cdk7	A	T	13: 100,848,062 (GRCm39)	Y153*	probably null	Het
Cemip	A	G	7: 83,613,218 (GRCm39)	I660T	probably benign	Het
Cfap65	A	T	1: 74,964,599 (GRCm39)	L518Q	probably damaging	Het
Cgas	T	A	9: 78,340,534 (GRCm39)	K399N	probably damaging	Het
Cngb3	A	G	4: 19,366,467 (GRCm39)	H176R	probably benign	Het
Cstdc4	T	C	16: 36,004,648 (GRCm39)	S7P	probably damaging	Het
Cux1	T	A	5: 136,308,351 (GRCm39)	I1263F	probably damaging	Het
Dnajc13	A	G	9: 104,044,258 (GRCm39)	M1867T	probably benign	Het
Dock2	A	G	11: 34,388,327 (GRCm39)	L202P	probably damaging	Het
Dyrk3	A	G	1: 131,057,769 (GRCm39)	S100P	probably benign	Het
Efr3b	A	T	12: 4,027,923 (GRCm39)	S376T	probably benign	Het
Eps8l2	A	C	7: 140,936,112 (GRCm39)	N222T	probably benign	Het
Ermp1	A	T	19: 29,608,806 (GRCm39)	Y158N	probably damaging	Het
Fam13a	A	G	6: 58,964,159 (GRCm39)	V91A	probably benign	Het
Fat3	A	G	9: 15,909,699 (GRCm39)	V2101A	possibly damaging	Het
Fsip2	T	C	2: 82,805,849 (GRCm39)	S723P	possibly damaging	Het
Garem1	G	T	18: 21,432,801 (GRCm39)	C9*	probably null	Het
Gdpd5	A	G	7: 99,107,997 (GRCm39)	I530V	possibly damaging	Het
Gm15217	T	A	14: 46,617,841 (GRCm39)		probably benign	Het
Gm4922	T	C	10: 18,659,289 (GRCm39)	T478A	probably benign	Het
H2-M5	A	G	17: 37,298,328 (GRCm39)	I329T	possibly damaging	Het
Ing4	G	A	6: 125,024,857 (GRCm39)	C200Y	probably damaging	Het
Kcnip1	A	T	11: 33,793,177 (GRCm39)	M5K	probably benign	Het
Kdsr	T	C	1: 106,675,517 (GRCm39)	E102G	probably damaging	Het
Krt15	C	T	11: 100,024,007 (GRCm39)	V346M	probably benign	Het
Lgr6	C	T	1: 134,921,748 (GRCm39)	A199T	probably damaging	Het
Lrrc55	A	T	2: 85,026,589 (GRCm39)	M145K	probably damaging	Het
Lrrtm2	A	G	18: 35,345,985 (GRCm39)	I439T	probably benign	Het
Map2k6	T	C	11: 110,390,335 (GRCm39)	F290L	probably damaging	Het
Me1	T	A	9: 86,533,055 (GRCm39)	I136F	probably damaging	Het
Mfap2	A	G	4: 140,742,294 (GRCm39)	D98G	probably damaging	Het
Mfsd13a	C	T	19: 46,354,943 (GRCm39)	T40I	probably benign	Het
Mst1	T	C	9: 107,962,096 (GRCm39)	F696L	probably damaging	Het
Myh15	A	T	16: 48,934,368 (GRCm39)	N645I	probably benign	Het
Myo7b	T	A	18: 32,147,262 (GRCm39)	I94F	probably damaging	Het
Nefh	A	T	11: 4,890,799 (GRCm39)	S607T	probably benign	Het
Noa1	G	A	5: 77,445,020 (GRCm39)	Q600*	probably null	Het
Nr2f2	A	G	7: 70,007,810 (GRCm39)	V71A	possibly damaging	Het
Oas2	A	T	5: 120,876,466 (GRCm39)	F492L	probably damaging	Het
Olfm3	T	A	3: 114,914,622 (GRCm39)	D211E	probably damaging	Het
Or2y17	A	T	11: 49,231,641 (GRCm39)	Y94F	probably benign	Het
Osmr	A	G	15: 6,871,432 (GRCm39)		probably null	Het
Plagl2	A	T	2: 153,073,523 (GRCm39)	D459E	probably benign	Het
Plch2	T	C	4: 155,091,168 (GRCm39)	D148G	possibly damaging	Het
Plxnc1	C	A	10: 94,700,869 (GRCm39)	C605F	probably damaging	Het
Ppm1m	T	C	9: 106,075,325 (GRCm39)	E108G	probably damaging	Het
Prr14l	A	C	5: 32,950,985 (GRCm39)	L1936R	probably damaging	Het
Ralgapa1	A	G	12: 55,723,354 (GRCm39)	I1771T	possibly damaging	Het
Rhobtb2	T	C	14: 70,033,357 (GRCm39)	T538A	probably benign	Het
Rictor	A	G	15: 6,813,588 (GRCm39)	N1025D	possibly damaging	Het
Sec23a	T	G	12: 59,037,804 (GRCm39)	D324A	probably damaging	Het
Srgap1	A	T	10: 121,883,097 (GRCm39)	M1K	probably null	Het
Syne2	T	A	12: 75,965,384 (GRCm39)	F801I	probably benign	Het
Synrg	T	A	11: 83,915,163 (GRCm39)		probably null	Het
Tas2r140	T	G	6: 40,468,232 (GRCm39)	F21V	probably benign	Het
Tmem260	A	T	14: 48,689,504 (GRCm39)	T108S	possibly damaging	Het
Trim2	T	C	3: 84,098,083 (GRCm39)	Y406C	probably damaging	Het
Ttn	T	C	2: 76,673,746 (GRCm39)		probably benign	Het
Vmn1r53	A	T	6: 90,201,064 (GRCm39)	S87T	possibly damaging	Het
Vmn2r115	T	A	17: 23,564,196 (GRCm39)	Y123N	probably benign	Het
Vmn2r28	T	A	7: 5,496,715 (GRCm39)	I46F	probably benign	Het
Zan	T	C	5: 137,395,028 (GRCm39)	T4381A	unknown	Het
Zfp457	A	G	13: 67,440,710 (GRCm39)	F622L	probably damaging	Het
Zfp994	A	T	17: 22,419,091 (GRCm39)	N619K	probably benign	Het
Zfy1	T	C	Y: 726,121 (GRCm39)	H548R	possibly damaging	Het
Zmym4	A	T	4: 126,804,938 (GRCm39)	S441T	probably benign	Het

Other mutations in Mta1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02227:Mta1	APN	12	113,084,528 (GRCm39)	missense	possibly damaging	0.94
IGL02250:Mta1	APN	12	113,090,418 (GRCm39)	missense	possibly damaging	0.59
IGL02391:Mta1	APN	12	113,100,203 (GRCm39)	missense	possibly damaging	0.79
IGL02670:Mta1	APN	12	113,093,741 (GRCm39)	missense	probably damaging	1.00
PIT4382001:Mta1	UTSW	12	113,096,870 (GRCm39)	missense	probably benign	0.06
R0496:Mta1	UTSW	12	113,094,941 (GRCm39)	nonsense	probably null
R1774:Mta1	UTSW	12	113,091,659 (GRCm39)	missense	probably damaging	1.00
R1870:Mta1	UTSW	12	113,091,694 (GRCm39)	missense	possibly damaging	0.73
R1976:Mta1	UTSW	12	113,099,926 (GRCm39)	missense	probably damaging	0.97
R2110:Mta1	UTSW	12	113,095,248 (GRCm39)	missense	probably damaging	1.00
R2111:Mta1	UTSW	12	113,095,248 (GRCm39)	missense	probably damaging	1.00
R2184:Mta1	UTSW	12	113,093,815 (GRCm39)	critical splice donor site	probably null
R2274:Mta1	UTSW	12	113,091,770 (GRCm39)	missense	probably damaging	1.00
R4087:Mta1	UTSW	12	113,075,802 (GRCm39)	missense	probably damaging	1.00
R4231:Mta1	UTSW	12	113,099,447 (GRCm39)	missense	possibly damaging	0.95
R4916:Mta1	UTSW	12	113,100,160 (GRCm39)	missense	probably benign	0.17
R5032:Mta1	UTSW	12	113,097,145 (GRCm39)	splice site	probably null
R5271:Mta1	UTSW	12	113,095,577 (GRCm39)	missense	probably damaging	0.99
R5344:Mta1	UTSW	12	113,095,186 (GRCm39)	splice site	probably benign
R5392:Mta1	UTSW	12	113,096,856 (GRCm39)	missense	probably benign
R5656:Mta1	UTSW	12	113,086,759 (GRCm39)	missense	probably damaging	1.00
R5903:Mta1	UTSW	12	113,100,239 (GRCm39)	missense	probably damaging	1.00
R6168:Mta1	UTSW	12	113,086,739 (GRCm39)	missense	probably damaging	0.96
R7091:Mta1	UTSW	12	113,100,022 (GRCm39)	missense	probably damaging	1.00
R7334:Mta1	UTSW	12	113,090,418 (GRCm39)	missense	possibly damaging	0.59
R7408:Mta1	UTSW	12	113,095,088 (GRCm39)	critical splice donor site	probably null
R7889:Mta1	UTSW	12	113,095,308 (GRCm39)	missense	probably benign	0.02
R8136:Mta1	UTSW	12	113,095,298 (GRCm39)	missense	probably damaging	1.00
R8176:Mta1	UTSW	12	113,084,456 (GRCm39)	missense	probably benign	0.00
R8385:Mta1	UTSW	12	113,095,085 (GRCm39)	missense	probably benign
R8398:Mta1	UTSW	12	113,095,242 (GRCm39)	missense	possibly damaging	0.83
R9132:Mta1	UTSW	12	113,100,025 (GRCm39)	missense	probably damaging	1.00
R9159:Mta1	UTSW	12	113,100,025 (GRCm39)	missense	probably damaging	1.00
R9418:Mta1	UTSW	12	113,094,987 (GRCm39)	missense	probably damaging	1.00
R9489:Mta1	UTSW	12	113,095,085 (GRCm39)	missense	probably benign
R9596:Mta1	UTSW	12	113,090,470 (GRCm39)	missense	probably damaging	0.99
R9682:Mta1	UTSW	12	113,095,384 (GRCm39)	critical splice donor site	probably null
Z1088:Mta1	UTSW	12	113,096,820 (GRCm39)	missense	probably benign	0.25

Predicted Primers

PCR Primer
(F):5'- GTACTGCGTTTCTGAACCACACCC -3'
(R):5'- ATGCGAGCAGTCACAGCGCAATAG -3'

Sequencing Primer
(F):5'- CTTACCCTTTCATTTCCACAGAGAC -3'
(R):5'- TCGTGCAAGGACAGTCCATC -3'

Posted On

2013-04-24