Mutagenetix > Incidental Mutation

Incidental Mutation 'R0373:Akap13'

30610

Institutional Source

Beutler Lab

Gene Symbol

Akap13

Ensembl Gene

ENSMUSG00000066406

Gene Name

A kinase (PRKA) anchor protein 13

Synonyms

AKAP-Lbc, 5730522G15Rik, 1700026G02Rik, PROTO-LBC, PROTO-LB, Ht31, 5830460E08Rik

MMRRC Submission

038579-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0373 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

75455534-75754609 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 75730500 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 2193 (S2193T)

Ref Sequence

ENSEMBL: ENSMUSP00000147237 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166315] [ENSMUST00000207239] [ENSMUST00000207750]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000147005
AA Change: S2193T

PolyPhen 2 Score 0.018 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000117686
Gene: ENSMUSG00000066406
AA Change: S2193T

Domain	Start	End	E-Value	Type
low complexity region	174	184	N/A	INTRINSIC
internal_repeat_1	485	695	4.85e-5	PROSPERO
low complexity region	773	789	N/A	INTRINSIC
low complexity region	896	908	N/A	INTRINSIC
low complexity region	1021	1032	N/A	INTRINSIC
Pfam:RII_binding_1	1218	1235	4.3e-6	PFAM
low complexity region	1429	1444	N/A	INTRINSIC
low complexity region	1479	1501	N/A	INTRINSIC
low complexity region	1601	1612	N/A	INTRINSIC
low complexity region	1738	1768	N/A	INTRINSIC
C1	1773	1819	1.95e-4	SMART
low complexity region	1876	1887	N/A	INTRINSIC
RhoGEF	1979	2171	1.28e-61	SMART
PH	2213	2316	2.94e-11	SMART
coiled coil region	2326	2363	N/A	INTRINSIC
low complexity region	2411	2430	N/A	INTRINSIC
low complexity region	2462	2472	N/A	INTRINSIC
coiled coil region	2551	2664	N/A	INTRINSIC
low complexity region	2746	2752	N/A	INTRINSIC
low complexity region	2758	2771	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000166315
AA Change: S2175T

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000129784
Gene: ENSMUSG00000066406
AA Change: S2175T

Domain	Start	End	E-Value	Type
low complexity region	174	184	N/A	INTRINSIC
low complexity region	773	789	N/A	INTRINSIC
low complexity region	896	908	N/A	INTRINSIC
low complexity region	1021	1032	N/A	INTRINSIC
low complexity region	1433	1448	N/A	INTRINSIC
low complexity region	1483	1505	N/A	INTRINSIC
low complexity region	1583	1594	N/A	INTRINSIC
low complexity region	1720	1750	N/A	INTRINSIC
C1	1755	1801	1.95e-4	SMART
low complexity region	1858	1869	N/A	INTRINSIC
RhoGEF	1961	2153	1.28e-61	SMART
PH	2195	2298	2.94e-11	SMART
coiled coil region	2308	2345	N/A	INTRINSIC
low complexity region	2393	2412	N/A	INTRINSIC
low complexity region	2444	2454	N/A	INTRINSIC
coiled coil region	2533	2646	N/A	INTRINSIC
low complexity region	2728	2734	N/A	INTRINSIC
low complexity region	2740	2753	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000207239
AA Change: S474T

PolyPhen 2 Score 0.978 (Sensitivity: 0.76; Specificity: 0.96)

Predicted Effect

probably damaging
Transcript: ENSMUST00000207750
AA Change: S2193T

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

Coding Region Coverage

1x: 99.1%
3x: 98.1%
10x: 95.8%
20x: 91.5%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms containing c-terminal dbl oncogene homology (DH) and pleckstrin homology (PH) domains. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins, resulting in the conversion of the inactive GTPase to the active form capable of transducing signals. The PH domain has multiple functions. Therefore, these isoforms function as scaffolding proteins to coordinate a Rho signaling pathway, function as protein kinase A-anchoring proteins and, in addition, enhance ligand-dependent activity of estrogen receptors alpha and beta. [provided by RefSeq, Jul 2012]
PHENOTYPE: Mice homozygous for a null allele exhibit embryonic lethality during organogenesis, arrested heart development, and forebrain hypoplasia. Heterozygous mice exhibit small spleen, impaired lymphocyte response to osmotic stress, decreased response to glucocorticoid, osteoporosis and impared osteogenesis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 92 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930579F01Rik	A	T	3: 138,173,582 (GRCm38)	L235Q	probably damaging	Het
Aadacl4fm4	A	T	4: 144,686,220 (GRCm38)	M50K	possibly damaging	Het
Adam6b	T	A	12: 113,490,655 (GRCm38)	V364D	probably benign	Het
Anapc11	T	C	11: 120,605,377 (GRCm38)	V69A	probably benign	Het
Ankmy1	C	T	1: 92,896,190 (GRCm38)	R118Q	probably damaging	Het
Ankrd27	T	C	7: 35,638,053 (GRCm38)	S931P	probably benign	Het
Atp6v1c2	G	A	12: 17,288,168 (GRCm38)	R280C	probably damaging	Het
Bbs10	T	A	10: 111,300,052 (GRCm38)	I342N	probably damaging	Het
Calhm2	T	C	19: 47,132,950 (GRCm38)	D260G	possibly damaging	Het
Camk2a	A	G	18: 60,958,238 (GRCm38)	E264G	probably damaging	Het
Ccdc146	T	A	5: 21,319,545 (GRCm38)	M270L	probably benign	Het
Cdc16	A	G	8: 13,779,264 (GRCm38)	T517A	probably benign	Het
Ces1g	T	C	8: 93,331,193 (GRCm38)	H160R	probably benign	Het
Chst4	T	C	8: 110,030,394 (GRCm38)	N196S	probably damaging	Het
Ciz1	A	T	2: 32,367,467 (GRCm38)	N175Y	probably damaging	Het
Cyb5r4	G	A	9: 87,027,040 (GRCm38)	V57I	probably damaging	Het
Cyth3	A	G	5: 143,684,426 (GRCm38)		probably benign	Het
Def6	A	G	17: 28,220,180 (GRCm38)	E255G	probably damaging	Het
Dhtkd1	T	G	2: 5,911,870 (GRCm38)	Q665P	probably damaging	Het
Dsg3	A	C	18: 20,539,747 (GRCm38)	D825A	probably damaging	Het
Eif3m	T	C	2: 105,005,000 (GRCm38)	T242A	probably benign	Het
Emilin3	A	G	2: 160,909,817 (GRCm38)	F101L	probably benign	Het
Epha7	A	G	4: 28,935,700 (GRCm38)		probably null	Het
Fbxo45	A	T	16: 32,238,405 (GRCm38)	Y224N	probably damaging	Het
Fhod3	A	T	18: 25,090,104 (GRCm38)	M836L	possibly damaging	Het
Fut4	C	A	9: 14,751,210 (GRCm38)	V263F	probably damaging	Het
Ggt1	C	T	10: 75,579,270 (GRCm38)	T206M	probably benign	Het
Gls	T	C	1: 52,188,699 (GRCm38)	R79G	probably damaging	Het
Grhl1	T	C	12: 24,581,515 (GRCm38)	S156P	probably benign	Het
Ipo8	C	T	6: 148,775,042 (GRCm38)	S983N	probably benign	Het
Kcna7	C	T	7: 45,409,444 (GRCm38)	A385V	probably damaging	Het
Kpnb1	A	T	11: 97,185,090 (GRCm38)	L40Q	probably damaging	Het
Matn1	A	T	4: 130,950,106 (GRCm38)	S209C	probably damaging	Het
Mcc	A	G	18: 44,475,222 (GRCm38)	I501T	probably benign	Het
Mdp1	A	T	14: 55,659,375 (GRCm38)	F104L	probably damaging	Het
Mib2	A	T	4: 155,656,288 (GRCm38)	N626K	probably damaging	Het
Mrgprh	T	C	17: 12,876,956 (GRCm38)	S28P	possibly damaging	Het
Mup-ps23	T	A	4: 61,856,149 (GRCm38)		noncoding transcript	Het
Myh15	A	G	16: 49,182,959 (GRCm38)	T1794A	possibly damaging	Het
Myo18a	C	G	11: 77,821,042 (GRCm38)	P680A	probably benign	Het
Myom2	G	T	8: 15,098,419 (GRCm38)	D532Y	possibly damaging	Het
Ndufaf5	A	G	2: 140,170,881 (GRCm38)	N57S	probably benign	Het
Nectin3	C	T	16: 46,458,187 (GRCm38)	V282M	probably damaging	Het
Nup188	G	T	2: 30,330,988 (GRCm38)	D997Y	probably damaging	Het
Olfm3	T	C	3: 115,122,805 (GRCm38)	V462A	probably damaging	Het
Opcml	A	G	9: 28,813,398 (GRCm38)	H164R	possibly damaging	Het
Or14a259	A	T	7: 86,363,805 (GRCm38)	C177*	probably null	Het
Or4c120	A	T	2: 89,170,413 (GRCm38)	F266L	probably benign	Het
Or8u9	A	C	2: 86,171,706 (GRCm38)	F37C	probably damaging	Het
Pacrg	A	G	17: 10,403,418 (GRCm38)	I209T	probably damaging	Het
Pcf11	T	C	7: 92,661,215 (GRCm38)	M522V	probably benign	Het
Pck1	T	A	2: 173,153,390 (GRCm38)	M1K	probably null	Het
Pcm1	G	T	8: 41,276,111 (GRCm38)	E707*	probably null	Het
Pcsk5	G	A	19: 17,654,849 (GRCm38)	R318W	probably damaging	Het
Phf11d	A	T	14: 59,353,344 (GRCm38)	M188K	possibly damaging	Het
Ppip5k2	A	T	1: 97,740,537 (GRCm38)	C615*	probably null	Het
Prkdc	T	A	16: 15,791,927 (GRCm38)	S3132T	probably damaging	Het
Prl2c5	A	T	13: 13,183,024 (GRCm38)		probably benign	Het
Prpsap2	A	G	11: 61,741,000 (GRCm38)	I177T	possibly damaging	Het
Rad50	A	G	11: 53,650,519 (GRCm38)	S1297P	probably damaging	Het
Rasip1	T	A	7: 45,635,244 (GRCm38)	N678K	possibly damaging	Het
Rubcn	A	G	16: 32,835,980 (GRCm38)	S544P	probably damaging	Het
Rwdd2a	A	T	9: 86,574,400 (GRCm38)	T210S	possibly damaging	Het
Scd2	A	G	19: 44,303,040 (GRCm38)	D306G	probably damaging	Het
Sema3b	T	C	9: 107,602,918 (GRCm38)	N207S	probably benign	Het
Sf3b2	C	T	19: 5,274,824 (GRCm38)	D845N	probably damaging	Het
Sipa1l2	C	A	8: 125,464,410 (GRCm38)	C947F	probably damaging	Het
Slc12a1	A	T	2: 125,226,031 (GRCm38)	T1013S	probably damaging	Het
Slc18a2	A	T	19: 59,287,367 (GRCm38)	I461L	probably benign	Het
Slc1a6	C	A	10: 78,801,922 (GRCm38)	Y427*	probably null	Het
Slc30a4	A	T	2: 122,689,399 (GRCm38)	I231K	probably damaging	Het
Sos1	G	T	17: 80,453,763 (GRCm38)	A168D	probably damaging	Het
Spata31f1a	T	C	4: 42,851,161 (GRCm38)	I332V	probably benign	Het
Sptb	T	C	12: 76,621,371 (GRCm38)	S651G	probably benign	Het
Stk36	T	C	1: 74,633,620 (GRCm38)	L1007P	probably damaging	Het
Tek	A	T	4: 94,804,341 (GRCm38)	N229Y	probably damaging	Het
Tep1	A	G	14: 50,836,768 (GRCm38)	F1887L	possibly damaging	Het
Tet1	A	T	10: 62,878,209 (GRCm38)	C602*	probably null	Het
Tnfrsf19	A	G	14: 60,972,036 (GRCm38)	S262P	possibly damaging	Het
Trim5	T	C	7: 104,265,684 (GRCm38)	I393V	probably benign	Het
Trpm6	A	G	19: 18,853,587 (GRCm38)	E1272G	probably benign	Het
Ttc21b	A	T	2: 66,188,326 (GRCm38)	Y1246N	probably damaging	Het
Ttll3	T	A	6: 113,398,777 (GRCm38)	L151H	probably damaging	Het
U2surp	C	T	9: 95,484,443 (GRCm38)	V470I	probably benign	Het
Ubr1	A	T	2: 120,946,657 (GRCm38)	Y276N	probably benign	Het
Uggt1	A	G	1: 36,179,670 (GRCm38)	S59P	probably benign	Het
Unc45a	T	C	7: 80,326,344 (GRCm38)	T796A	probably damaging	Het
Unc5b	C	A	10: 60,778,940 (GRCm38)	V193F	possibly damaging	Het
Upp1	G	T	11: 9,129,590 (GRCm38)	M50I	probably benign	Het
Vps18	C	T	2: 119,293,905 (GRCm38)	R438C	probably damaging	Het
Zfp715	T	C	7: 43,299,336 (GRCm38)	Y400C	possibly damaging	Het
Zfp955b	T	C	17: 33,302,522 (GRCm38)	Y322H	probably benign	Het

Other mutations in Akap13

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00161:Akap13	APN	7	75,725,971 (GRCm38)	missense	probably damaging	0.99
IGL00332:Akap13	APN	7	75,728,919 (GRCm38)	missense	probably damaging	1.00
IGL00481:Akap13	APN	7	75,723,895 (GRCm38)	missense	probably damaging	1.00
IGL00590:Akap13	APN	7	75,610,669 (GRCm38)	missense	probably benign	0.01
IGL00655:Akap13	APN	7	75,704,398 (GRCm38)	missense	probably damaging	0.99
IGL00766:Akap13	APN	7	75,704,512 (GRCm38)	missense	probably damaging	0.96
IGL00818:Akap13	APN	7	75,609,727 (GRCm38)	missense	probably benign	0.00
IGL00826:Akap13	APN	7	75,677,447 (GRCm38)	missense	probably damaging	1.00
IGL01014:Akap13	APN	7	75,750,633 (GRCm38)	utr 3 prime	probably benign
IGL01090:Akap13	APN	7	75,666,531 (GRCm38)	missense	probably benign	0.44
IGL01155:Akap13	APN	7	75,569,936 (GRCm38)	missense	probably damaging	1.00
IGL01326:Akap13	APN	7	75,725,348 (GRCm38)	missense	probably benign	0.30
IGL01456:Akap13	APN	7	75,602,847 (GRCm38)	missense	probably damaging	0.98
IGL01460:Akap13	APN	7	75,747,846 (GRCm38)	missense	probably benign	0.29
IGL01568:Akap13	APN	7	75,608,522 (GRCm38)	nonsense	probably null	0.00
IGL01610:Akap13	APN	7	75,747,605 (GRCm38)	missense	probably damaging	1.00
IGL01610:Akap13	APN	7	75,720,180 (GRCm38)	missense	possibly damaging	0.71
IGL01615:Akap13	APN	7	75,697,393 (GRCm38)	missense	probably damaging	1.00
IGL01667:Akap13	APN	7	75,570,019 (GRCm38)	missense	probably damaging	1.00
IGL01705:Akap13	APN	7	75,746,767 (GRCm38)	missense	possibly damaging	0.86
IGL02070:Akap13	APN	7	75,666,545 (GRCm38)	missense	probably benign	0.27
IGL02269:Akap13	APN	7	75,602,911 (GRCm38)	missense	probably benign
IGL02421:Akap13	APN	7	75,717,806 (GRCm38)	missense	possibly damaging	0.66
IGL02870:Akap13	APN	7	75,609,188 (GRCm38)	missense	probably damaging	0.96
IGL02944:Akap13	APN	7	75,608,657 (GRCm38)	missense	probably benign
IGL03051:Akap13	APN	7	75,610,485 (GRCm38)	nonsense	probably null
IGL03160:Akap13	APN	7	75,730,417 (GRCm38)	missense	probably damaging	1.00
IGL03245:Akap13	APN	7	75,609,752 (GRCm38)	missense	probably damaging	0.99
R0254:Akap13	UTSW	7	75,736,604 (GRCm38)	splice site	probably benign
R0310:Akap13	UTSW	7	75,614,930 (GRCm38)	missense	probably damaging	0.99
R0373:Akap13	UTSW	7	75,609,929 (GRCm38)	missense	probably benign	0.00
R0408:Akap13	UTSW	7	75,746,796 (GRCm38)	missense	probably damaging	1.00
R0631:Akap13	UTSW	7	75,614,996 (GRCm38)	missense	probably damaging	0.99
R0646:Akap13	UTSW	7	75,747,746 (GRCm38)	missense	probably damaging	1.00
R0781:Akap13	UTSW	7	75,611,377 (GRCm38)	missense	possibly damaging	0.56
R0845:Akap13	UTSW	7	75,725,380 (GRCm38)	missense	probably damaging	1.00
R1004:Akap13	UTSW	7	75,687,286 (GRCm38)	missense	probably damaging	0.99
R1024:Akap13	UTSW	7	75,677,409 (GRCm38)	missense	probably damaging	1.00
R1110:Akap13	UTSW	7	75,611,377 (GRCm38)	missense	possibly damaging	0.56
R1346:Akap13	UTSW	7	75,609,592 (GRCm38)	missense	possibly damaging	0.67
R1349:Akap13	UTSW	7	75,609,592 (GRCm38)	missense	possibly damaging	0.67
R1372:Akap13	UTSW	7	75,609,592 (GRCm38)	missense	possibly damaging	0.67
R1387:Akap13	UTSW	7	75,586,193 (GRCm38)	missense	probably damaging	0.97
R1442:Akap13	UTSW	7	75,735,778 (GRCm38)	missense	probably damaging	0.99
R1466:Akap13	UTSW	7	75,729,049 (GRCm38)	missense	possibly damaging	0.79
R1466:Akap13	UTSW	7	75,729,049 (GRCm38)	missense	possibly damaging	0.79
R1584:Akap13	UTSW	7	75,729,049 (GRCm38)	missense	possibly damaging	0.79
R1696:Akap13	UTSW	7	75,609,592 (GRCm38)	missense	possibly damaging	0.67
R1738:Akap13	UTSW	7	75,677,194 (GRCm38)	missense	probably damaging	1.00
R1773:Akap13	UTSW	7	75,683,451 (GRCm38)	missense	possibly damaging	0.80
R1785:Akap13	UTSW	7	75,611,434 (GRCm38)	missense	probably benign	0.16
R1786:Akap13	UTSW	7	75,611,434 (GRCm38)	missense	probably benign	0.16
R1791:Akap13	UTSW	7	75,611,035 (GRCm38)	missense	probably benign	0.00
R1819:Akap13	UTSW	7	75,608,705 (GRCm38)	missense	probably benign	0.04
R1879:Akap13	UTSW	7	75,610,727 (GRCm38)	missense	probably benign	0.01
R1989:Akap13	UTSW	7	75,704,516 (GRCm38)	missense	probably benign	0.01
R2016:Akap13	UTSW	7	75,704,531 (GRCm38)	missense	probably damaging	0.99
R2092:Akap13	UTSW	7	75,610,570 (GRCm38)	missense	probably benign	0.05
R2126:Akap13	UTSW	7	75,725,304 (GRCm38)	missense	possibly damaging	0.95
R2131:Akap13	UTSW	7	75,611,434 (GRCm38)	missense	probably benign	0.16
R2132:Akap13	UTSW	7	75,611,434 (GRCm38)	missense	probably benign	0.16
R2133:Akap13	UTSW	7	75,611,434 (GRCm38)	missense	probably benign	0.16
R2251:Akap13	UTSW	7	75,739,477 (GRCm38)	missense	possibly damaging	0.50
R3704:Akap13	UTSW	7	75,666,550 (GRCm38)	missense	probably damaging	1.00
R3713:Akap13	UTSW	7	75,586,181 (GRCm38)	missense	probably damaging	0.98
R3731:Akap13	UTSW	7	75,611,377 (GRCm38)	missense	probably benign	0.39
R3765:Akap13	UTSW	7	75,608,837 (GRCm38)	missense	probably benign	0.04
R3788:Akap13	UTSW	7	75,702,153 (GRCm38)	critical splice donor site	probably null
R3793:Akap13	UTSW	7	75,610,141 (GRCm38)	missense	probably benign	0.00
R3970:Akap13	UTSW	7	75,569,951 (GRCm38)	nonsense	probably null
R4205:Akap13	UTSW	7	75,610,919 (GRCm38)	missense	probably benign	0.05
R4257:Akap13	UTSW	7	75,611,285 (GRCm38)	missense	probably damaging	0.98
R4374:Akap13	UTSW	7	75,608,984 (GRCm38)	missense	probably damaging	0.96
R4448:Akap13	UTSW	7	75,742,760 (GRCm38)	missense	probably damaging	1.00
R4450:Akap13	UTSW	7	75,742,760 (GRCm38)	missense	probably damaging	1.00
R4457:Akap13	UTSW	7	75,739,465 (GRCm38)	missense	probably damaging	0.99
R4458:Akap13	UTSW	7	75,739,465 (GRCm38)	missense	probably damaging	0.99
R4466:Akap13	UTSW	7	75,602,773 (GRCm38)	splice site	probably null
R4632:Akap13	UTSW	7	75,666,553 (GRCm38)	missense	possibly damaging	0.91
R4667:Akap13	UTSW	7	75,729,094 (GRCm38)	missense	probably damaging	1.00
R4669:Akap13	UTSW	7	75,729,094 (GRCm38)	missense	probably damaging	1.00
R4671:Akap13	UTSW	7	75,579,564 (GRCm38)	nonsense	probably null
R4821:Akap13	UTSW	7	75,677,507 (GRCm38)	intron	probably benign
R4868:Akap13	UTSW	7	75,743,504 (GRCm38)	missense	probably damaging	1.00
R4894:Akap13	UTSW	7	75,725,320 (GRCm38)	missense	possibly damaging	0.76
R4943:Akap13	UTSW	7	75,749,240 (GRCm38)	missense	probably benign	0.22
R4962:Akap13	UTSW	7	75,749,430 (GRCm38)	missense	probably damaging	0.98
R4988:Akap13	UTSW	7	75,730,528 (GRCm38)	missense	probably damaging	1.00
R5119:Akap13	UTSW	7	75,687,252 (GRCm38)	missense	probably damaging	0.98
R5141:Akap13	UTSW	7	75,609,614 (GRCm38)	missense	probably benign	0.18
R5419:Akap13	UTSW	7	75,610,243 (GRCm38)	missense	probably benign	0.01
R5427:Akap13	UTSW	7	75,728,869 (GRCm38)	missense	possibly damaging	0.89
R5429:Akap13	UTSW	7	75,602,904 (GRCm38)	missense	possibly damaging	0.70
R5432:Akap13	UTSW	7	75,602,830 (GRCm38)	missense	probably damaging	1.00
R5458:Akap13	UTSW	7	75,586,301 (GRCm38)	missense	probably damaging	1.00
R5636:Akap13	UTSW	7	75,704,372 (GRCm38)	missense	probably damaging	0.96
R5643:Akap13	UTSW	7	75,702,154 (GRCm38)	critical splice donor site	probably null
R5898:Akap13	UTSW	7	75,729,146 (GRCm38)	missense	probably damaging	1.00
R5932:Akap13	UTSW	7	75,610,184 (GRCm38)	missense	probably damaging	1.00
R6135:Akap13	UTSW	7	75,609,908 (GRCm38)	missense	possibly damaging	0.94
R6137:Akap13	UTSW	7	75,677,416 (GRCm38)	missense	probably damaging	1.00
R6182:Akap13	UTSW	7	75,586,280 (GRCm38)	missense	probably benign	0.45
R6310:Akap13	UTSW	7	75,749,193 (GRCm38)	missense	probably damaging	0.99
R6346:Akap13	UTSW	7	75,685,254 (GRCm38)	missense	probably damaging	1.00
R6466:Akap13	UTSW	7	75,727,044 (GRCm38)	missense	probably benign	0.01
R6605:Akap13	UTSW	7	75,579,768 (GRCm38)	missense	probably damaging	0.98
R6617:Akap13	UTSW	7	75,730,363 (GRCm38)	missense	possibly damaging	0.95
R6621:Akap13	UTSW	7	75,569,981 (GRCm38)	missense	probably damaging	1.00
R6703:Akap13	UTSW	7	75,602,898 (GRCm38)	missense	probably damaging	1.00
R6750:Akap13	UTSW	7	75,739,458 (GRCm38)	missense	probably benign	0.03
R7069:Akap13	UTSW	7	75,610,262 (GRCm38)	missense	probably benign	0.29
R7116:Akap13	UTSW	7	75,720,195 (GRCm38)	missense	probably benign	0.00
R7158:Akap13	UTSW	7	75,579,594 (GRCm38)	missense	probably damaging	0.97
R7159:Akap13	UTSW	7	75,730,579 (GRCm38)	missense	possibly damaging	0.72
R7467:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R7468:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R7471:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R7472:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R7477:Akap13	UTSW	7	75,749,247 (GRCm38)	missense	probably benign
R7636:Akap13	UTSW	7	75,609,873 (GRCm38)	missense	probably benign	0.04
R7650:Akap13	UTSW	7	75,643,454 (GRCm38)	missense	probably benign	0.20
R7671:Akap13	UTSW	7	75,569,900 (GRCm38)	missense	probably damaging	1.00
R7681:Akap13	UTSW	7	75,728,796 (GRCm38)	missense	possibly damaging	0.91
R7752:Akap13	UTSW	7	75,677,258 (GRCm38)	missense	possibly damaging	0.74
R7784:Akap13	UTSW	7	75,610,328 (GRCm38)	missense	probably benign	0.00
R7816:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R7817:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R7834:Akap13	UTSW	7	75,742,642 (GRCm38)	missense	possibly damaging	0.85
R7880:Akap13	UTSW	7	75,586,216 (GRCm38)	missense	probably damaging	0.97
R7942:Akap13	UTSW	7	75,611,470 (GRCm38)	missense	possibly damaging	0.50
R8006:Akap13	UTSW	7	75,579,696 (GRCm38)	missense	probably damaging	1.00
R8009:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R8011:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R8012:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R8013:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R8016:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R8089:Akap13	UTSW	7	75,610,592 (GRCm38)	missense	possibly damaging	0.94
R8138:Akap13	UTSW	7	75,702,231 (GRCm38)	splice site	probably null
R8174:Akap13	UTSW	7	75,728,869 (GRCm38)	missense	possibly damaging	0.89
R8298:Akap13	UTSW	7	75,747,804 (GRCm38)	missense	probably damaging	1.00
R8444:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R8445:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R8465:Akap13	UTSW	7	75,727,038 (GRCm38)	missense	probably benign	0.11
R8512:Akap13	UTSW	7	75,611,086 (GRCm38)	missense	probably damaging	0.99
R8523:Akap13	UTSW	7	75,730,465 (GRCm38)	missense	probably damaging	1.00
R8793:Akap13	UTSW	7	75,725,328 (GRCm38)	missense	probably benign	0.35
R8907:Akap13	UTSW	7	75,610,708 (GRCm38)	missense	probably damaging	0.99
R8907:Akap13	UTSW	7	75,610,696 (GRCm38)	missense	probably benign	0.08
R8928:Akap13	UTSW	7	75,609,858 (GRCm38)	missense	probably benign	0.00
R8929:Akap13	UTSW	7	75,609,004 (GRCm38)	missense	probably benign	0.00
R8937:Akap13	UTSW	7	75,534,853 (GRCm38)	critical splice donor site	probably null
R8967:Akap13	UTSW	7	75,729,134 (GRCm38)	missense	possibly damaging	0.80
R8986:Akap13	UTSW	7	75,609,326 (GRCm38)	missense	probably benign
R9152:Akap13	UTSW	7	75,611,285 (GRCm38)	missense	probably damaging	0.98
R9153:Akap13	UTSW	7	75,609,481 (GRCm38)	missense	probably benign	0.00
R9160:Akap13	UTSW	7	75,735,778 (GRCm38)	missense	possibly damaging	0.88
R9192:Akap13	UTSW	7	75,704,501 (GRCm38)	missense	probably benign	0.06
R9319:Akap13	UTSW	7	75,609,088 (GRCm38)	missense	probably benign	0.01
R9513:Akap13	UTSW	7	75,704,527 (GRCm38)	missense	probably benign	0.01
R9515:Akap13	UTSW	7	75,704,527 (GRCm38)	missense	probably benign	0.01
R9516:Akap13	UTSW	7	75,704,527 (GRCm38)	missense	probably benign	0.01
R9523:Akap13	UTSW	7	75,643,445 (GRCm38)	missense
R9564:Akap13	UTSW	7	75,609,413 (GRCm38)	missense	probably benign
R9621:Akap13	UTSW	7	75,736,342 (GRCm38)	missense	probably benign	0.09
R9686:Akap13	UTSW	7	75,586,336 (GRCm38)	missense	probably damaging	1.00
Z1176:Akap13	UTSW	7	75,730,552 (GRCm38)	missense	probably damaging	0.99
Z1177:Akap13	UTSW	7	75,615,005 (GRCm38)	missense	probably benign	0.17

Predicted Primers

PCR Primer
(F):5'- CTCTCCCAAGACAATGAAGTGGAGC -3'
(R):5'- TGCCACTGACCTGATCCCACAATG -3'

Sequencing Primer
(F):5'- GGAGCAGGAAGACCTCACTC -3'
(R):5'- GGAGTATGTCTTCCCCAACAG -3'

Posted On

2013-04-24