Incidental Mutation 'R0373:Opcml'
ID 30622
Institutional Source Beutler Lab
Gene Symbol Opcml
Ensembl Gene ENSMUSG00000062257
Gene Name opioid binding protein/cell adhesion molecule-like
Synonyms Obcam, B930023M13Rik, 2900075O15Rik, LOC235104
MMRRC Submission 038579-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.079) question?
Stock # R0373 (G1)
Quality Score 225
Status Not validated
Chromosome 9
Chromosomal Location 27790775-28925410 bp(+) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) A to G at 28813398 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Histidine to Arginine at position 164 (H164R)
Ref Sequence ENSEMBL: ENSMUSP00000110898 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000073822] [ENSMUST00000115243]
AlphaFold G5E8G3
Predicted Effect possibly damaging
Transcript: ENSMUST00000073822
AA Change: H171R

PolyPhen 2 Score 0.479 (Sensitivity: 0.89; Specificity: 0.90)
SMART Domains Protein: ENSMUSP00000073493
Gene: ENSMUSG00000062257
AA Change: H171R

signal peptide 1 18 N/A INTRINSIC
IG 42 133 2.94e-10 SMART
IGc2 148 209 2.91e-14 SMART
IGc2 235 303 2e-12 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000115243
AA Change: H164R

PolyPhen 2 Score 0.479 (Sensitivity: 0.89; Specificity: 0.90)
SMART Domains Protein: ENSMUSP00000110898
Gene: ENSMUSG00000062257
AA Change: H164R

IG 35 126 2.94e-10 SMART
IGc2 141 201 1.36e-14 SMART
IGc2 227 295 2e-12 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000126673
Predicted Effect noncoding transcript
Transcript: ENSMUST00000215483
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.1%
  • 10x: 95.8%
  • 20x: 91.5%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the IgLON subfamily in the immunoglobulin protein superfamily of proteins. The encoded preprotein is proteolytically processed to generate the mature protein. This protein is localized in the plasma membrane and may have an accessory role in opioid receptor function. This gene has an ortholog in rat and bovine. The opioid binding-cell adhesion molecule encoded by the rat gene binds opioid alkaloids in the presence of acidic lipids, exhibits selectivity for mu ligands and acts as a GPI-anchored protein. Since the encoded protein is highly conserved in species during evolution, it may have a fundamental role in mammalian systems. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]
Allele List at MGI
Other mutations in this stock
Total: 93 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930579F01Rik A T 3: 138,173,582 (GRCm38) L235Q probably damaging Het
Aadacl4fm4 A T 4: 144,686,220 (GRCm38) M50K possibly damaging Het
Adam6b T A 12: 113,490,655 (GRCm38) V364D probably benign Het
Akap13 T A 7: 75,730,500 (GRCm38) S2193T probably damaging Het
Akap13 T C 7: 75,609,929 (GRCm38) L767P probably benign Het
Anapc11 T C 11: 120,605,377 (GRCm38) V69A probably benign Het
Ankmy1 C T 1: 92,896,190 (GRCm38) R118Q probably damaging Het
Ankrd27 T C 7: 35,638,053 (GRCm38) S931P probably benign Het
Atp6v1c2 G A 12: 17,288,168 (GRCm38) R280C probably damaging Het
Bbs10 T A 10: 111,300,052 (GRCm38) I342N probably damaging Het
Calhm2 T C 19: 47,132,950 (GRCm38) D260G possibly damaging Het
Camk2a A G 18: 60,958,238 (GRCm38) E264G probably damaging Het
Ccdc146 T A 5: 21,319,545 (GRCm38) M270L probably benign Het
Cdc16 A G 8: 13,779,264 (GRCm38) T517A probably benign Het
Ces1g T C 8: 93,331,193 (GRCm38) H160R probably benign Het
Chst4 T C 8: 110,030,394 (GRCm38) N196S probably damaging Het
Ciz1 A T 2: 32,367,467 (GRCm38) N175Y probably damaging Het
Cyb5r4 G A 9: 87,027,040 (GRCm38) V57I probably damaging Het
Cyth3 A G 5: 143,684,426 (GRCm38) probably benign Het
Def6 A G 17: 28,220,180 (GRCm38) E255G probably damaging Het
Dhtkd1 T G 2: 5,911,870 (GRCm38) Q665P probably damaging Het
Dsg3 A C 18: 20,539,747 (GRCm38) D825A probably damaging Het
Eif3m T C 2: 105,005,000 (GRCm38) T242A probably benign Het
Emilin3 A G 2: 160,909,817 (GRCm38) F101L probably benign Het
Epha7 A G 4: 28,935,700 (GRCm38) probably null Het
Fbxo45 A T 16: 32,238,405 (GRCm38) Y224N probably damaging Het
Fhod3 A T 18: 25,090,104 (GRCm38) M836L possibly damaging Het
Fut4 C A 9: 14,751,210 (GRCm38) V263F probably damaging Het
Ggt1 C T 10: 75,579,270 (GRCm38) T206M probably benign Het
Gls T C 1: 52,188,699 (GRCm38) R79G probably damaging Het
Grhl1 T C 12: 24,581,515 (GRCm38) S156P probably benign Het
Ipo8 C T 6: 148,775,042 (GRCm38) S983N probably benign Het
Kcna7 C T 7: 45,409,444 (GRCm38) A385V probably damaging Het
Kpnb1 A T 11: 97,185,090 (GRCm38) L40Q probably damaging Het
Matn1 A T 4: 130,950,106 (GRCm38) S209C probably damaging Het
Mcc A G 18: 44,475,222 (GRCm38) I501T probably benign Het
Mdp1 A T 14: 55,659,375 (GRCm38) F104L probably damaging Het
Mib2 A T 4: 155,656,288 (GRCm38) N626K probably damaging Het
Mrgprh T C 17: 12,876,956 (GRCm38) S28P possibly damaging Het
Mup-ps23 T A 4: 61,856,149 (GRCm38) noncoding transcript Het
Myh15 A G 16: 49,182,959 (GRCm38) T1794A possibly damaging Het
Myo18a C G 11: 77,821,042 (GRCm38) P680A probably benign Het
Myom2 G T 8: 15,098,419 (GRCm38) D532Y possibly damaging Het
Ndufaf5 A G 2: 140,170,881 (GRCm38) N57S probably benign Het
Nectin3 C T 16: 46,458,187 (GRCm38) V282M probably damaging Het
Nup188 G T 2: 30,330,988 (GRCm38) D997Y probably damaging Het
Olfm3 T C 3: 115,122,805 (GRCm38) V462A probably damaging Het
Or14a259 A T 7: 86,363,805 (GRCm38) C177* probably null Het
Or4c120 A T 2: 89,170,413 (GRCm38) F266L probably benign Het
Or8u9 A C 2: 86,171,706 (GRCm38) F37C probably damaging Het
Pacrg A G 17: 10,403,418 (GRCm38) I209T probably damaging Het
Pcf11 T C 7: 92,661,215 (GRCm38) M522V probably benign Het
Pck1 T A 2: 173,153,390 (GRCm38) M1K probably null Het
Pcm1 G T 8: 41,276,111 (GRCm38) E707* probably null Het
Pcsk5 G A 19: 17,654,849 (GRCm38) R318W probably damaging Het
Phf11d A T 14: 59,353,344 (GRCm38) M188K possibly damaging Het
Ppip5k2 A T 1: 97,740,537 (GRCm38) C615* probably null Het
Prkdc T A 16: 15,791,927 (GRCm38) S3132T probably damaging Het
Prl2c5 A T 13: 13,183,024 (GRCm38) probably benign Het
Prpsap2 A G 11: 61,741,000 (GRCm38) I177T possibly damaging Het
Rad50 A G 11: 53,650,519 (GRCm38) S1297P probably damaging Het
Rasip1 T A 7: 45,635,244 (GRCm38) N678K possibly damaging Het
Rubcn A G 16: 32,835,980 (GRCm38) S544P probably damaging Het
Rwdd2a A T 9: 86,574,400 (GRCm38) T210S possibly damaging Het
Scd2 A G 19: 44,303,040 (GRCm38) D306G probably damaging Het
Sema3b T C 9: 107,602,918 (GRCm38) N207S probably benign Het
Sf3b2 C T 19: 5,274,824 (GRCm38) D845N probably damaging Het
Sipa1l2 C A 8: 125,464,410 (GRCm38) C947F probably damaging Het
Slc12a1 A T 2: 125,226,031 (GRCm38) T1013S probably damaging Het
Slc18a2 A T 19: 59,287,367 (GRCm38) I461L probably benign Het
Slc1a6 C A 10: 78,801,922 (GRCm38) Y427* probably null Het
Slc30a4 A T 2: 122,689,399 (GRCm38) I231K probably damaging Het
Sos1 G T 17: 80,453,763 (GRCm38) A168D probably damaging Het
Spata31f1a T C 4: 42,851,161 (GRCm38) I332V probably benign Het
Sptb T C 12: 76,621,371 (GRCm38) S651G probably benign Het
Stk36 T C 1: 74,633,620 (GRCm38) L1007P probably damaging Het
Tek A T 4: 94,804,341 (GRCm38) N229Y probably damaging Het
Tep1 A G 14: 50,836,768 (GRCm38) F1887L possibly damaging Het
Tet1 A T 10: 62,878,209 (GRCm38) C602* probably null Het
Tnfrsf19 A G 14: 60,972,036 (GRCm38) S262P possibly damaging Het
Trim5 T C 7: 104,265,684 (GRCm38) I393V probably benign Het
Trpm6 A G 19: 18,853,587 (GRCm38) E1272G probably benign Het
Ttc21b A T 2: 66,188,326 (GRCm38) Y1246N probably damaging Het
Ttll3 T A 6: 113,398,777 (GRCm38) L151H probably damaging Het
U2surp C T 9: 95,484,443 (GRCm38) V470I probably benign Het
Ubr1 A T 2: 120,946,657 (GRCm38) Y276N probably benign Het
Uggt1 A G 1: 36,179,670 (GRCm38) S59P probably benign Het
Unc45a T C 7: 80,326,344 (GRCm38) T796A probably damaging Het
Unc5b C A 10: 60,778,940 (GRCm38) V193F possibly damaging Het
Upp1 G T 11: 9,129,590 (GRCm38) M50I probably benign Het
Vps18 C T 2: 119,293,905 (GRCm38) R438C probably damaging Het
Zfp715 T C 7: 43,299,336 (GRCm38) Y400C possibly damaging Het
Zfp955b T C 17: 33,302,522 (GRCm38) Y322H probably benign Het
Other mutations in Opcml
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00864:Opcml APN 9 28,901,591 (GRCm38) missense probably damaging 1.00
IGL00864:Opcml APN 9 28,901,590 (GRCm38) missense probably damaging 1.00
IGL00956:Opcml APN 9 28,675,328 (GRCm38) missense possibly damaging 0.86
IGL02391:Opcml APN 9 28,675,264 (GRCm38) missense probably damaging 0.96
IGL03210:Opcml APN 9 28,901,537 (GRCm38) missense probably damaging 0.99
R1037:Opcml UTSW 9 28,903,299 (GRCm38) missense probably damaging 1.00
R1564:Opcml UTSW 9 28,903,316 (GRCm38) missense probably damaging 1.00
R2094:Opcml UTSW 9 28,901,590 (GRCm38) missense probably damaging 1.00
R2268:Opcml UTSW 9 28,903,355 (GRCm38) missense possibly damaging 0.91
R2426:Opcml UTSW 9 28,903,367 (GRCm38) critical splice donor site probably null
R2938:Opcml UTSW 9 27,791,386 (GRCm38) start codon destroyed probably null 0.00
R3746:Opcml UTSW 9 28,901,530 (GRCm38) missense possibly damaging 0.54
R4058:Opcml UTSW 9 28,901,588 (GRCm38) missense probably damaging 1.00
R4173:Opcml UTSW 9 28,903,358 (GRCm38) missense probably benign
R4882:Opcml UTSW 9 28,901,590 (GRCm38) missense probably damaging 1.00
R5335:Opcml UTSW 9 28,675,325 (GRCm38) missense possibly damaging 0.88
R7058:Opcml UTSW 9 28,675,211 (GRCm38) nonsense probably null
R7559:Opcml UTSW 9 28,903,324 (GRCm38) missense probably benign
R8050:Opcml UTSW 9 28,813,344 (GRCm38) missense probably damaging 0.97
R8250:Opcml UTSW 9 28,675,270 (GRCm38) missense probably damaging 1.00
R8350:Opcml UTSW 9 28,902,167 (GRCm38) missense probably benign 0.00
R8772:Opcml UTSW 9 27,791,411 (GRCm38) missense probably benign 0.04
R8879:Opcml UTSW 9 28,902,151 (GRCm38) missense probably damaging 1.00
R9355:Opcml UTSW 9 28,903,354 (GRCm38) missense probably benign 0.00
R9364:Opcml UTSW 9 28,903,328 (GRCm38) missense probably damaging 1.00
R9385:Opcml UTSW 9 28,675,163 (GRCm38) missense possibly damaging 0.62
Z1177:Opcml UTSW 9 28,404,377 (GRCm38) missense probably damaging 0.99
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2013-04-24