Mutagenetix > Incidental Mutation

Incidental Mutation 'R0375:Or10x4'

30669

Institutional Source

Beutler Lab

Gene Symbol

Or10x4

Ensembl Gene

ENSMUSG00000059503

Gene Name

olfactory receptor family 10 subfamily X member 4

Synonyms

MOR267-7, GA_x6K02T2MFC0-1145-1312, GA_x6K02T2P20D-20771141-20770212, Olfr415, Olfr248

MMRRC Submission

038581-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.104) question?

Stock #

R0375 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

174218612-174219621 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 174218775 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 47 (T47A)

Ref Sequence

ENSEMBL: ENSMUSP00000074799 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000075329]

AlphaFold

E9Q1L6

Predicted Effect

probably damaging
Transcript: ENSMUST00000075329
AA Change: T47A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000074799
Gene: ENSMUSG00000059503
AA Change: T47A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	304	1.2e-40	PFAM
Pfam:7tm_1	39	303	7.5e-22	PFAM

Meta Mutation Damage Score

0.2296

Coding Region Coverage

1x: 99.2%
3x: 98.3%
10x: 96.2%
20x: 92.7%

Validation Efficiency

100% (61/61)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5730522E02Rik	T	A	11: 25,719,092 (GRCm39)	Y17F	unknown	Het
Aars2	A	G	17: 45,825,476 (GRCm39)	D313G	probably damaging	Het
Abca9	A	T	11: 110,006,273 (GRCm39)	D1277E	probably benign	Het
Adgrl1	G	T	8: 84,661,530 (GRCm39)	A981S	probably damaging	Het
Aff3	T	G	1: 38,244,021 (GRCm39)	K917Q	possibly damaging	Het
BC049715	A	T	6: 136,816,994 (GRCm39)	H78L	probably benign	Het
Bltp1	C	T	3: 37,100,401 (GRCm39)	T4707I	probably damaging	Het
Cacna1g	C	A	11: 94,301,880 (GRCm39)	A2027S	possibly damaging	Het
Camk1g	G	A	1: 193,038,709 (GRCm39)		probably benign	Het
Carf	T	C	1: 60,183,161 (GRCm39)	V386A	probably damaging	Het
Cd46	A	G	1: 194,768,472 (GRCm39)	S82P	probably benign	Het
Clic5	A	G	17: 44,581,510 (GRCm39)	E180G	possibly damaging	Het
Col27a1	A	G	4: 63,143,898 (GRCm39)	T529A	probably benign	Het
Col7a1	C	T	9: 108,809,305 (GRCm39)	R2627C	unknown	Het
Cuzd1	G	A	7: 130,913,637 (GRCm39)		probably benign	Het
Cwc27	T	C	13: 104,944,331 (GRCm39)	D50G	possibly damaging	Het
Dhx38	A	G	8: 110,281,813 (GRCm39)	V735A	possibly damaging	Het
Dhx57	T	G	17: 80,565,550 (GRCm39)	E834A	probably damaging	Het
Dsg4	A	G	18: 20,603,936 (GRCm39)	D801G	probably damaging	Het
Dtx1	T	C	5: 120,819,464 (GRCm39)	E578G	probably damaging	Het
F830016B08Rik	A	T	18: 60,433,265 (GRCm39)	H116L	probably damaging	Het
Fbxw18	T	C	9: 109,517,907 (GRCm39)	I360V	possibly damaging	Het
Fignl2	G	A	15: 100,951,974 (GRCm39)	P103S	probably benign	Het
Frmpd1	A	G	4: 45,284,196 (GRCm39)	T1006A	probably benign	Het
Ggnbp2	G	T	11: 84,727,200 (GRCm39)	C545*	probably null	Het
Gm3336	A	G	8: 71,171,294 (GRCm39)		probably benign	Het
Gpr37	T	C	6: 25,669,290 (GRCm39)	N518S	probably benign	Het
Hbs1l	T	A	10: 21,218,440 (GRCm39)	D312E	possibly damaging	Het
Hoxd10	C	A	2: 74,523,064 (GRCm39)	S247R	probably benign	Het
Ifnb1	A	T	4: 88,440,981 (GRCm39)	F11I	probably benign	Het
Marf1	T	C	16: 13,969,184 (GRCm39)		probably benign	Het
Myo1f	T	A	17: 33,820,930 (GRCm39)	V879E	probably benign	Het
Naip1	A	G	13: 100,545,656 (GRCm39)	F1291L	probably benign	Het
Nckap1l	A	G	15: 103,382,586 (GRCm39)	E529G	probably damaging	Het
Npm3	T	C	19: 45,736,668 (GRCm39)	E157G	probably damaging	Het
Or4a67	T	A	2: 88,597,985 (GRCm39)	T225S	possibly damaging	Het
Or4a68	G	T	2: 89,269,740 (GRCm39)	N294K	probably benign	Het
Pex16	G	A	2: 92,210,802 (GRCm39)	G312D	probably damaging	Het
Ppp6r1	A	G	7: 4,636,286 (GRCm39)	V768A	probably benign	Het
Prrc1	A	G	18: 57,495,564 (GRCm39)	T14A	probably damaging	Het
Ranbp2	T	C	10: 58,313,105 (GRCm39)	L1275P	probably damaging	Het
Rnf214	C	A	9: 45,811,121 (GRCm39)	V181F	probably damaging	Het
Ror2	T	C	13: 53,286,040 (GRCm39)	N58S	probably damaging	Het
Selplg	G	A	5: 113,958,069 (GRCm39)	T79I	probably damaging	Het
Setd1b	G	A	5: 123,295,500 (GRCm39)	G1023S	unknown	Het
Sf3b2	C	T	19: 5,324,852 (GRCm39)	D845N	probably damaging	Het
Skint5	A	G	4: 113,562,793 (GRCm39)	V803A	unknown	Het
Slc25a46	T	C	18: 31,716,319 (GRCm39)	I394M	possibly damaging	Het
Snrnp27	A	T	6: 86,657,935 (GRCm39)	I101K	possibly damaging	Het
Spag17	C	A	3: 99,934,906 (GRCm39)	T704K	probably benign	Het
Tas2r144	T	A	6: 42,193,058 (GRCm39)	M266K	possibly damaging	Het
Tasor2	A	G	13: 3,646,842 (GRCm39)	V61A	possibly damaging	Het
Tbc1d31	T	A	15: 57,818,746 (GRCm39)	L783H	probably benign	Het
Tbck	C	A	3: 132,456,993 (GRCm39)		probably benign	Het
Vcan	A	G	13: 89,839,394 (GRCm39)	V2050A	probably damaging	Het
Vmn1r70	T	A	7: 10,367,987 (GRCm39)	N158K	probably damaging	Het
Vmn2r103	T	A	17: 20,013,121 (GRCm39)	Y81N	probably benign	Het
Vmn2r103	A	G	17: 20,013,726 (GRCm39)	T173A	probably benign	Het
Ylpm1	T	G	12: 85,061,754 (GRCm39)	S552A	unknown	Het
Zdhhc17	A	T	10: 110,817,967 (GRCm39)	Y58*	probably null	Het

Other mutations in Or10x4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01649:Or10x4	APN	1	174,218,974 (GRCm39)	missense	probably damaging	1.00
R1720:Or10x4	UTSW	1	174,219,486 (GRCm39)	missense	probably benign	0.00
R1906:Or10x4	UTSW	1	174,218,730 (GRCm39)	missense	probably damaging	1.00
R1996:Or10x4	UTSW	1	174,218,983 (GRCm39)	missense	probably damaging	1.00
R2009:Or10x4	UTSW	1	174,218,995 (GRCm39)	missense	possibly damaging	0.94
R4745:Or10x4	UTSW	1	174,219,442 (GRCm39)	missense	probably damaging	1.00
R5294:Or10x4	UTSW	1	174,218,791 (GRCm39)	missense	probably benign
R5297:Or10x4	UTSW	1	174,218,766 (GRCm39)	missense	probably benign	0.21
R5665:Or10x4	UTSW	1	174,218,941 (GRCm39)	missense	probably damaging	1.00
R5784:Or10x4	UTSW	1	174,218,965 (GRCm39)	missense	probably damaging	1.00
R5857:Or10x4	UTSW	1	174,218,674 (GRCm39)	missense	possibly damaging	0.59
R6943:Or10x4	UTSW	1	174,219,407 (GRCm39)	nonsense	probably null
R6975:Or10x4	UTSW	1	174,219,243 (GRCm39)	missense	probably benign	0.10
R7114:Or10x4	UTSW	1	174,218,805 (GRCm39)	missense	probably damaging	0.96
R7614:Or10x4	UTSW	1	174,219,220 (GRCm39)	missense	probably damaging	0.99
R8036:Or10x4	UTSW	1	174,219,382 (GRCm39)	missense	probably damaging	1.00
R8674:Or10x4	UTSW	1	174,219,258 (GRCm39)	missense	probably damaging	1.00
R8777:Or10x4	UTSW	1	174,218,848 (GRCm39)	missense	probably damaging	1.00
R8777-TAIL:Or10x4	UTSW	1	174,218,848 (GRCm39)	missense	probably damaging	1.00
R9139:Or10x4	UTSW	1	174,218,649 (GRCm39)	missense	probably damaging	1.00
R9178:Or10x4	UTSW	1	174,219,229 (GRCm39)	missense	probably benign	0.36
R9256:Or10x4	UTSW	1	174,219,109 (GRCm39)	missense	probably benign	0.11
R9449:Or10x4	UTSW	1	174,218,742 (GRCm39)	missense	probably benign
R9668:Or10x4	UTSW	1	174,218,898 (GRCm39)	missense	probably benign	0.04

Predicted Primers

PCR Primer
(F):5'- CGTCTACATACAGGGTTGAGTTTTGCC -3'
(R):5'- AGCCACAGCCTATGTCTGAAATGC -3'

Sequencing Primer
(F):5'- GGTTGAGTTTTGCCAAACTGAATAG -3'
(R):5'- CCTATGTCTGAAATGCGGTTGTC -3'

Posted On

2013-04-24