Incidental Mutation 'R0375:Or4a68'
| ID |
30674 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Or4a68
|
| Ensembl Gene |
ENSMUSG00000111174 |
| Gene Name |
olfactory receptor family 4 subfamily A member 68 |
| Synonyms |
Olfr1240, MOR231-8, GA_x6K02T2Q125-50883183-50882239 |
| MMRRC Submission |
038581-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.093)
|
| Stock # |
R0375 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
2 |
| Chromosomal Location |
89269614-89270687 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
G to T
at 89269740 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Asparagine to Lysine
at position 294
(N294K)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000150256
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000215362]
[ENSMUST00000216123]
|
| AlphaFold |
Q8VG73 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000099779
AA Change: N294K
PolyPhen 2
Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
|
| SMART Domains |
Protein: ENSMUSP00000097367
Gene: ENSMUSG00000075087
AA Change: N294K
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:7tm_4
|
29 |
303 |
4e-45 |
PFAM |
|
Pfam:7tm_1
|
39 |
285 |
1.7e-18 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000187363
AA Change: N294K
PolyPhen 2
Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
|
| SMART Domains |
Protein: ENSMUSP00000139968
Gene: ENSMUSG00000099909
AA Change: N294K
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:7tm_1
|
39 |
285 |
2e-28 |
PFAM |
|
Pfam:7tm_4
|
137 |
278 |
1.2e-37 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000215362
AA Change: N294K
PolyPhen 2
Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000216123
AA Change: N294K
PolyPhen 2
Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
|
| Meta Mutation Damage Score |
0.0898 |
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.3%
- 10x: 96.2%
- 20x: 92.7%
|
| Validation Efficiency |
100% (61/61) |
| MGI Phenotype |
FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 60 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 5730522E02Rik |
T |
A |
11: 25,719,092 (GRCm39) |
Y17F |
unknown |
Het |
| Aars2 |
A |
G |
17: 45,825,476 (GRCm39) |
D313G |
probably damaging |
Het |
| Abca9 |
A |
T |
11: 110,006,273 (GRCm39) |
D1277E |
probably benign |
Het |
| Adgrl1 |
G |
T |
8: 84,661,530 (GRCm39) |
A981S |
probably damaging |
Het |
| Aff3 |
T |
G |
1: 38,244,021 (GRCm39) |
K917Q |
possibly damaging |
Het |
| BC049715 |
A |
T |
6: 136,816,994 (GRCm39) |
H78L |
probably benign |
Het |
| Bltp1 |
C |
T |
3: 37,100,401 (GRCm39) |
T4707I |
probably damaging |
Het |
| Cacna1g |
C |
A |
11: 94,301,880 (GRCm39) |
A2027S |
possibly damaging |
Het |
| Camk1g |
G |
A |
1: 193,038,709 (GRCm39) |
|
probably benign |
Het |
| Carf |
T |
C |
1: 60,183,161 (GRCm39) |
V386A |
probably damaging |
Het |
| Cd46 |
A |
G |
1: 194,768,472 (GRCm39) |
S82P |
probably benign |
Het |
| Clic5 |
A |
G |
17: 44,581,510 (GRCm39) |
E180G |
possibly damaging |
Het |
| Col27a1 |
A |
G |
4: 63,143,898 (GRCm39) |
T529A |
probably benign |
Het |
| Col7a1 |
C |
T |
9: 108,809,305 (GRCm39) |
R2627C |
unknown |
Het |
| Cuzd1 |
G |
A |
7: 130,913,637 (GRCm39) |
|
probably benign |
Het |
| Cwc27 |
T |
C |
13: 104,944,331 (GRCm39) |
D50G |
possibly damaging |
Het |
| Dhx38 |
A |
G |
8: 110,281,813 (GRCm39) |
V735A |
possibly damaging |
Het |
| Dhx57 |
T |
G |
17: 80,565,550 (GRCm39) |
E834A |
probably damaging |
Het |
| Dsg4 |
A |
G |
18: 20,603,936 (GRCm39) |
D801G |
probably damaging |
Het |
| Dtx1 |
T |
C |
5: 120,819,464 (GRCm39) |
E578G |
probably damaging |
Het |
| F830016B08Rik |
A |
T |
18: 60,433,265 (GRCm39) |
H116L |
probably damaging |
Het |
| Fbxw18 |
T |
C |
9: 109,517,907 (GRCm39) |
I360V |
possibly damaging |
Het |
| Fignl2 |
G |
A |
15: 100,951,974 (GRCm39) |
P103S |
probably benign |
Het |
| Frmpd1 |
A |
G |
4: 45,284,196 (GRCm39) |
T1006A |
probably benign |
Het |
| Ggnbp2 |
G |
T |
11: 84,727,200 (GRCm39) |
C545* |
probably null |
Het |
| Gm3336 |
A |
G |
8: 71,171,294 (GRCm39) |
|
probably benign |
Het |
| Gpr37 |
T |
C |
6: 25,669,290 (GRCm39) |
N518S |
probably benign |
Het |
| Hbs1l |
T |
A |
10: 21,218,440 (GRCm39) |
D312E |
possibly damaging |
Het |
| Hoxd10 |
C |
A |
2: 74,523,064 (GRCm39) |
S247R |
probably benign |
Het |
| Ifnb1 |
A |
T |
4: 88,440,981 (GRCm39) |
F11I |
probably benign |
Het |
| Marf1 |
T |
C |
16: 13,969,184 (GRCm39) |
|
probably benign |
Het |
| Myo1f |
T |
A |
17: 33,820,930 (GRCm39) |
V879E |
probably benign |
Het |
| Naip1 |
A |
G |
13: 100,545,656 (GRCm39) |
F1291L |
probably benign |
Het |
| Nckap1l |
A |
G |
15: 103,382,586 (GRCm39) |
E529G |
probably damaging |
Het |
| Npm3 |
T |
C |
19: 45,736,668 (GRCm39) |
E157G |
probably damaging |
Het |
| Or10x4 |
A |
G |
1: 174,218,775 (GRCm39) |
T47A |
probably damaging |
Het |
| Or4a67 |
T |
A |
2: 88,597,985 (GRCm39) |
T225S |
possibly damaging |
Het |
| Pex16 |
G |
A |
2: 92,210,802 (GRCm39) |
G312D |
probably damaging |
Het |
| Ppp6r1 |
A |
G |
7: 4,636,286 (GRCm39) |
V768A |
probably benign |
Het |
| Prrc1 |
A |
G |
18: 57,495,564 (GRCm39) |
T14A |
probably damaging |
Het |
| Ranbp2 |
T |
C |
10: 58,313,105 (GRCm39) |
L1275P |
probably damaging |
Het |
| Rnf214 |
C |
A |
9: 45,811,121 (GRCm39) |
V181F |
probably damaging |
Het |
| Ror2 |
T |
C |
13: 53,286,040 (GRCm39) |
N58S |
probably damaging |
Het |
| Selplg |
G |
A |
5: 113,958,069 (GRCm39) |
T79I |
probably damaging |
Het |
| Setd1b |
G |
A |
5: 123,295,500 (GRCm39) |
G1023S |
unknown |
Het |
| Sf3b2 |
C |
T |
19: 5,324,852 (GRCm39) |
D845N |
probably damaging |
Het |
| Skint5 |
A |
G |
4: 113,562,793 (GRCm39) |
V803A |
unknown |
Het |
| Slc25a46 |
T |
C |
18: 31,716,319 (GRCm39) |
I394M |
possibly damaging |
Het |
| Snrnp27 |
A |
T |
6: 86,657,935 (GRCm39) |
I101K |
possibly damaging |
Het |
| Spag17 |
C |
A |
3: 99,934,906 (GRCm39) |
T704K |
probably benign |
Het |
| Tas2r144 |
T |
A |
6: 42,193,058 (GRCm39) |
M266K |
possibly damaging |
Het |
| Tasor2 |
A |
G |
13: 3,646,842 (GRCm39) |
V61A |
possibly damaging |
Het |
| Tbc1d31 |
T |
A |
15: 57,818,746 (GRCm39) |
L783H |
probably benign |
Het |
| Tbck |
C |
A |
3: 132,456,993 (GRCm39) |
|
probably benign |
Het |
| Vcan |
A |
G |
13: 89,839,394 (GRCm39) |
V2050A |
probably damaging |
Het |
| Vmn1r70 |
T |
A |
7: 10,367,987 (GRCm39) |
N158K |
probably damaging |
Het |
| Vmn2r103 |
T |
A |
17: 20,013,121 (GRCm39) |
Y81N |
probably benign |
Het |
| Vmn2r103 |
A |
G |
17: 20,013,726 (GRCm39) |
T173A |
probably benign |
Het |
| Ylpm1 |
T |
G |
12: 85,061,754 (GRCm39) |
S552A |
unknown |
Het |
| Zdhhc17 |
A |
T |
10: 110,817,967 (GRCm39) |
Y58* |
probably null |
Het |
|
| Other mutations in Or4a68 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01765:Or4a68
|
APN |
2 |
89,270,144 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL01767:Or4a68
|
APN |
2 |
89,270,144 (GRCm39) |
missense |
probably benign |
0.00 |
|
R0417:Or4a68
|
UTSW |
2 |
89,270,519 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R0501:Or4a68
|
UTSW |
2 |
89,270,060 (GRCm39) |
missense |
probably benign |
0.09 |
|
R0586:Or4a68
|
UTSW |
2 |
89,269,698 (GRCm39) |
missense |
possibly damaging |
0.55 |
|
R0624:Or4a68
|
UTSW |
2 |
89,270,482 (GRCm39) |
missense |
possibly damaging |
0.94 |
|
R1168:Or4a68
|
UTSW |
2 |
89,270,213 (GRCm39) |
nonsense |
probably null |
|
|
R1728:Or4a68
|
UTSW |
2 |
89,269,927 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1729:Or4a68
|
UTSW |
2 |
89,269,927 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1730:Or4a68
|
UTSW |
2 |
89,269,927 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1739:Or4a68
|
UTSW |
2 |
89,269,927 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1762:Or4a68
|
UTSW |
2 |
89,269,927 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1783:Or4a68
|
UTSW |
2 |
89,269,927 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1785:Or4a68
|
UTSW |
2 |
89,269,927 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2038:Or4a68
|
UTSW |
2 |
89,269,689 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2049:Or4a68
|
UTSW |
2 |
89,269,927 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2069:Or4a68
|
UTSW |
2 |
89,269,927 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2140:Or4a68
|
UTSW |
2 |
89,269,927 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2142:Or4a68
|
UTSW |
2 |
89,269,927 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2215:Or4a68
|
UTSW |
2 |
89,270,381 (GRCm39) |
missense |
probably benign |
0.00 |
|
R3714:Or4a68
|
UTSW |
2 |
89,269,727 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4304:Or4a68
|
UTSW |
2 |
89,270,542 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R4437:Or4a68
|
UTSW |
2 |
89,269,698 (GRCm39) |
missense |
possibly damaging |
0.55 |
|
R4825:Or4a68
|
UTSW |
2 |
89,270,209 (GRCm39) |
missense |
probably benign |
0.01 |
|
R4857:Or4a68
|
UTSW |
2 |
89,269,967 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4865:Or4a68
|
UTSW |
2 |
89,270,003 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R5588:Or4a68
|
UTSW |
2 |
89,269,760 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5730:Or4a68
|
UTSW |
2 |
89,269,780 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7206:Or4a68
|
UTSW |
2 |
89,270,801 (GRCm39) |
start gained |
probably benign |
|
|
R7365:Or4a68
|
UTSW |
2 |
89,270,542 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7574:Or4a68
|
UTSW |
2 |
89,269,745 (GRCm39) |
missense |
possibly damaging |
0.94 |
|
R8007:Or4a68
|
UTSW |
2 |
89,270,684 (GRCm39) |
|
|
|
|
R8268:Or4a68
|
UTSW |
2 |
89,269,780 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8559:Or4a68
|
UTSW |
2 |
89,270,528 (GRCm39) |
missense |
probably benign |
0.03 |
|
R8812:Or4a68
|
UTSW |
2 |
89,270,209 (GRCm39) |
missense |
probably benign |
0.01 |
|
R9269:Or4a68
|
UTSW |
2 |
89,270,276 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R9534:Or4a68
|
UTSW |
2 |
89,269,999 (GRCm39) |
missense |
probably benign |
0.03 |
|
| Predicted Primers |
PCR Primer
(F):5'- AGGAATCCCCAAACTGAGCAGGTA -3'
(R):5'- GCTCCCACATCATAGTGGTTGTCC -3'
Sequencing Primer
(F):5'- GGTATAAAAATCTGCCATGCAGAC -3'
(R):5'- CATCATAGTGGTTGTCCTCTTTTTTG -3'
|
| Posted On |
2013-04-24 |
Incidental Mutation