|Institutional Source||Beutler Lab|
|Gene Name||selectin, platelet (p-selectin) ligand|
|Synonyms||Psgl-1, CD162, Psgl1|
|Essential gene?||Probably non essential (E-score: 0.059)|
|Stock #||R0375 (G1)|
|Chromosomal Location||113818536-113832644 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||G to A at 113820008 bp (GRCm38)|
|Amino Acid Change||Threonine to Isoleucine at position 79 (T79I)|
|Ref Sequence||ENSEMBL: ENSMUSP00000098436 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000100874] [ENSMUST00000199109]|
|AlphaFold||no structure available at present|
AA Change: T79I
PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)
AA Change: T79I
|Meta Mutation Damage Score||0.6467|
|Coding Region Coverage||
|Validation Efficiency||100% (61/61)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]
PHENOTYPE: Mice homozygous for a null allele exhibit neutrophillia and impaired leukocyte adhesion and rolling. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Selplg||
(F):5'- AGGTCTCTACCTCTGTGGATGCTG -3'
(R):5'- GCAGGCTAAACCATATCTCTGCTCC -3'
(F):5'- CTGGTTGGACGGTCTCTAC -3'
(R):5'- TGGCAACAGCCTTCAGC -3'