Mutagenetix > Incidental Mutation

Incidental Mutation 'R3938:Vmn1r35'

307270

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r35

Ensembl Gene

ENSMUSG00000060699

Gene Name

vomeronasal 1 receptor 35

Synonyms

V1rc12

MMRRC Submission

040825-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R3938 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

66655778-66656668 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 66656057 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Serine at position 204 (R204S)

Ref Sequence

ENSEMBL: ENSMUSP00000154707 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071414] [ENSMUST00000227346] [ENSMUST00000227354] [ENSMUST00000227749] [ENSMUST00000227961]

AlphaFold

Q8R2E2

Predicted Effect

probably damaging
Transcript: ENSMUST00000071414
AA Change: R204S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000071362
Gene: ENSMUSG00000060699
AA Change: R204S

Domain	Start	End	E-Value	Type
Pfam:V1R	28	293	1.2e-54	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000227346
AA Change: R37S

PolyPhen 2 Score 0.953 (Sensitivity: 0.79; Specificity: 0.95)

Predicted Effect

probably damaging
Transcript: ENSMUST00000227354
AA Change: R204S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000227749
AA Change: R204S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000227961
AA Change: R37S

PolyPhen 2 Score 0.953 (Sensitivity: 0.79; Specificity: 0.95)

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.1%

Validation Efficiency

98% (57/58)

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts1	C	T	16: 85,592,507 (GRCm39)	V634M	possibly damaging	Het
Arid4b	A	T	13: 14,361,513 (GRCm39)	N659I	probably benign	Het
BC049715	T	C	6: 136,817,453 (GRCm39)	I231T	possibly damaging	Het
Bmp4	T	C	14: 46,621,536 (GRCm39)	Y336C	probably damaging	Het
Ccdc30	G	A	4: 119,209,870 (GRCm39)	T293I	probably benign	Het
Chd5	A	T	4: 152,461,512 (GRCm39)	T1275S	probably benign	Het
Chtf8	A	T	8: 107,612,537 (GRCm39)	M134K	probably benign	Het
Col11a2	A	T	17: 34,258,599 (GRCm39)		probably benign	Het
Cyp8b1	A	G	9: 121,744,684 (GRCm39)	V216A	probably benign	Het
Dnah8	C	T	17: 31,073,911 (GRCm39)	T4527M	probably damaging	Het
Elavl3	A	G	9: 21,930,040 (GRCm39)	V288A	probably damaging	Het
Erap1	T	A	13: 74,816,147 (GRCm39)	L92Q	probably damaging	Het
Exoc3l	G	T	8: 106,020,037 (GRCm39)	P296H	probably damaging	Het
Fcgbpl1	C	T	7: 27,853,719 (GRCm39)	P1561L	probably damaging	Het
Foxl3	A	T	5: 138,806,723 (GRCm39)	N78Y	probably damaging	Het
Hacl1	T	C	14: 31,356,148 (GRCm39)		probably benign	Het
Hrnr	T	A	3: 93,230,162 (GRCm39)	N133K	probably benign	Het
Itgax	G	T	7: 127,735,445 (GRCm39)	R504S	possibly damaging	Het
Itgb4	A	G	11: 115,896,752 (GRCm39)	S1461G	possibly damaging	Het
Klkb1	G	T	8: 45,735,838 (GRCm39)	T175K	probably damaging	Het
Lrrk2	A	T	15: 91,596,983 (GRCm39)	D525V	possibly damaging	Het
Lrrk2	C	A	15: 91,662,707 (GRCm39)	T1912K	probably damaging	Het
Mdga1	G	T	17: 30,076,596 (GRCm39)	Q59K	probably damaging	Het
Mpp4	A	G	1: 59,163,842 (GRCm39)	V466A	possibly damaging	Het
Myh6	T	C	14: 55,200,512 (GRCm39)	D203G	probably benign	Het
Myo5b	T	C	18: 74,849,108 (GRCm39)	S1116P	probably damaging	Het
Nemp1	T	C	10: 127,531,342 (GRCm39)	L311P	probably damaging	Het
Nup205	A	G	6: 35,196,677 (GRCm39)	R1138G	probably damaging	Het
Nup54	A	G	5: 92,565,388 (GRCm39)	M443T	probably damaging	Het
Peak1	T	C	9: 56,167,649 (GRCm39)	E93G	probably benign	Het
Pirb	A	T	7: 3,720,637 (GRCm39)	L287Q	probably benign	Het
Plekhj1	A	G	10: 80,633,609 (GRCm39)	I76T	probably damaging	Het
Poll	A	G	19: 45,546,857 (GRCm39)		probably benign	Het
Ppp1r3a	A	T	6: 14,719,073 (GRCm39)	S614T	probably damaging	Het
Pygm	A	T	19: 6,442,980 (GRCm39)	I556F	probably benign	Het
Ranbp2	T	C	10: 58,312,294 (GRCm39)	F1005L	probably benign	Het
Rcor3	G	T	1: 191,785,385 (GRCm39)	T361K	possibly damaging	Het
Robo4	A	G	9: 37,313,313 (GRCm39)		probably benign	Het
Rps15	T	C	10: 80,129,673 (GRCm39)	V96A	probably benign	Het
Rrm2	T	A	12: 24,759,431 (GRCm39)	N55K	probably damaging	Het
Shank2	T	C	7: 143,682,112 (GRCm39)	Y382H	probably benign	Het
Slc25a10	G	T	11: 120,382,819 (GRCm39)	E3*	probably null	Het
Slc7a8	T	A	14: 54,973,298 (GRCm39)	E223V	probably benign	Het
Snx13	A	G	12: 35,194,096 (GRCm39)	K880E	probably benign	Het
Spinkl	T	G	18: 44,301,216 (GRCm39)	M41L	probably benign	Het
Srp54a	A	C	12: 55,136,042 (GRCm39)	N19T	probably benign	Het
Sun2	T	C	15: 79,618,356 (GRCm39)	K268E	probably benign	Het
Sytl1	G	A	4: 132,982,935 (GRCm39)	Q359*	probably null	Het
Tlr6	A	T	5: 65,110,938 (GRCm39)	F656L	probably damaging	Het
Tmem185a	C	T	X: 69,505,792 (GRCm39)		probably null	Het
Tmem45a2	C	T	16: 56,859,398 (GRCm39)	D278N	probably benign	Het
Trav7-1	T	C	14: 52,892,791 (GRCm39)		probably benign	Het
Trpc2	T	A	7: 101,742,781 (GRCm39)	M597K	probably damaging	Het
Ttc21a	A	G	9: 119,779,882 (GRCm39)		probably benign	Het
Usp9y	C	T	Y: 1,313,741 (GRCm39)	M2188I	probably damaging	Het
Utrn	C	A	10: 12,625,774 (GRCm39)		probably null	Het
Zfp955b	T	A	17: 33,524,390 (GRCm39)	Y59F	probably damaging	Het

Other mutations in Vmn1r35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00962:Vmn1r35	APN	6	66,656,361 (GRCm39)	missense	possibly damaging	0.90
IGL01417:Vmn1r35	APN	6	66,656,191 (GRCm39)	missense	probably benign	0.00
IGL01517:Vmn1r35	APN	6	66,656,434 (GRCm39)	missense	probably benign	0.01
IGL02142:Vmn1r35	APN	6	66,656,334 (GRCm39)	missense	probably damaging	1.00
IGL02178:Vmn1r35	APN	6	66,656,086 (GRCm39)	missense	probably damaging	1.00
IGL02383:Vmn1r35	APN	6	66,655,876 (GRCm39)	missense	probably damaging	1.00
IGL02383:Vmn1r35	APN	6	66,655,875 (GRCm39)	missense	probably damaging	1.00
IGL02493:Vmn1r35	APN	6	66,656,463 (GRCm39)	missense	possibly damaging	0.76
R0360:Vmn1r35	UTSW	6	66,655,827 (GRCm39)	missense	probably damaging	0.99
R0364:Vmn1r35	UTSW	6	66,655,827 (GRCm39)	missense	probably damaging	0.99
R0599:Vmn1r35	UTSW	6	66,656,497 (GRCm39)	missense	probably benign	0.06
R1447:Vmn1r35	UTSW	6	66,655,890 (GRCm39)	missense	probably benign	0.13
R1781:Vmn1r35	UTSW	6	66,656,550 (GRCm39)	missense	probably benign	0.24
R2096:Vmn1r35	UTSW	6	66,655,929 (GRCm39)	missense	possibly damaging	0.94
R2937:Vmn1r35	UTSW	6	66,655,950 (GRCm39)	missense	possibly damaging	0.78
R2938:Vmn1r35	UTSW	6	66,655,950 (GRCm39)	missense	possibly damaging	0.78
R3937:Vmn1r35	UTSW	6	66,656,057 (GRCm39)	missense	probably damaging	1.00
R4386:Vmn1r35	UTSW	6	66,656,573 (GRCm39)	nonsense	probably null
R5329:Vmn1r35	UTSW	6	66,656,490 (GRCm39)	nonsense	probably null
R6638:Vmn1r35	UTSW	6	66,655,848 (GRCm39)	missense	possibly damaging	0.63
R7175:Vmn1r35	UTSW	6	66,655,906 (GRCm39)	missense	probably benign	0.06
R7448:Vmn1r35	UTSW	6	66,656,219 (GRCm39)	start gained	probably benign
R7825:Vmn1r35	UTSW	6	66,656,443 (GRCm39)	missense	probably damaging	1.00
R9139:Vmn1r35	UTSW	6	66,655,933 (GRCm39)	missense	probably benign	0.10
R9415:Vmn1r35	UTSW	6	66,656,115 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TGTAGGATAGGCATTCATCACAAAC -3'
(R):5'- AGGATCATCTATACTGGTGCTTATACC -3'

Sequencing Primer
(F):5'- GGATAGGCATTCATCACAAACTTCTG -3'
(R):5'- ACATCATCAGGGGACTGA -3'

Posted On

2015-04-17