Mutagenetix > Incidental Mutation

Incidental Mutation 'R3943:Gpr158'

307525

Institutional Source

Beutler Lab

Gene Symbol

Gpr158

Ensembl Gene

ENSMUSG00000045967

Gene Name

G protein-coupled receptor 158

Synonyms

5330427M13Rik

MMRRC Submission

040924-MU

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3943 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

21367542-21830547 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 21368559 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Serine at position 102 (R102S)

Ref Sequence

ENSEMBL: ENSMUSP00000049708 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055946]

AlphaFold

Q8C419

Predicted Effect

possibly damaging
Transcript: ENSMUST00000055946
AA Change: R102S

PolyPhen 2 Score 0.650 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000049708
Gene: ENSMUSG00000045967
AA Change: R102S

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
low complexity region	110	125	N/A	INTRINSIC
SCOP:d1edmb_	313	359	5e-4	SMART
Blast:EGF	318	365	2e-27	BLAST
Pfam:7tm_3	426	669	1.2e-35	PFAM
low complexity region	840	863	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000133391

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000155760

Meta Mutation Damage Score

0.1232

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.7%

Validation Efficiency

98% (49/50)

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610507B11Rik	T	A	11: 78,269,524	L603*	probably null	Het
6030419C18Rik	AGAGGAGGAGGAGGAGG	AGAGGAGGAGGAGG	9: 58,499,432		probably benign	Het
Adnp	A	G	2: 168,185,060	I105T	possibly damaging	Het
Atf3	A	G	1: 191,171,516	I171T	possibly damaging	Het
Atp6v0a1	A	G	11: 101,055,517	I773V	probably benign	Het
Cad	T	A	5: 31,072,385		probably null	Het
Camkv	T	A	9: 107,947,983	D366E	possibly damaging	Het
Dnah8	T	A	17: 30,694,065		probably benign	Het
Dock7	A	G	4: 98,992,431	S932P	probably damaging	Het
Fam227a	C	T	15: 79,620,859		probably benign	Het
Fbp2	A	G	13: 62,841,793	F233S	possibly damaging	Het
Gm7534	T	C	4: 134,200,345	T366A	probably benign	Het
Gnptab	G	T	10: 88,433,894	V820L	probably benign	Het
Gpat4	G	A	8: 23,180,155	P286L	probably damaging	Het
Gramd1b	A	T	9: 40,531,044	M1K	probably null	Het
H2-T23	A	G	17: 36,030,643	V312A	probably benign	Het
Hltf	A	T	3: 20,092,744	I494F	probably damaging	Het
Il16	G	A	7: 83,652,015	L300F	probably damaging	Het
Kcnq1	T	C	7: 143,426,088	L657P	probably damaging	Het
Krt4	G	A	15: 101,921,250	T281M	probably benign	Het
Mia3	A	G	1: 183,358,783	F196S	possibly damaging	Het
Naip6	T	C	13: 100,294,739	T1197A	probably benign	Het
Nrxn2	T	A	19: 6,473,335	N204K	probably damaging	Het
Olfr845	T	A	9: 19,339,075	M205K	probably benign	Het
Rassf6	G	T	5: 90,604,326	Q258K	possibly damaging	Het
Rhpn1	T	C	15: 75,711,806	L380P	probably damaging	Het
Ribc2	A	G	15: 85,135,250	M78V	probably benign	Het
Shroom3	G	T	5: 92,943,086	V1151F	probably damaging	Het
Skint5	T	A	4: 113,942,753	H73L	probably damaging	Het
Smg6	G	A	11: 74,929,541	G213R	probably damaging	Het
Sspn	T	A	6: 145,934,357	W62R	probably damaging	Het
Stard9	A	G	2: 120,698,229	S1656G	probably benign	Het
Svep1	T	A	4: 58,084,807		probably null	Het
Tab1	T	C	15: 80,153,740	L258P	probably damaging	Het
Tmem59l	A	G	8: 70,487,301	L6S	unknown	Het
Tnrc6c	C	T	11: 117,723,529	R838W	probably damaging	Het
Trf	T	A	9: 103,222,952	I271F	probably benign	Het
Trpm7	A	T	2: 126,831,218	N538K	possibly damaging	Het
Usp28	T	A	9: 49,000,366	L25I	probably benign	Het
Vmn2r14	A	T	5: 109,216,064	I662N	probably damaging	Het
Vmn2r42	A	T	7: 8,194,780	F280Y	possibly damaging	Het
Vps8	A	T	16: 21,470,123	N411Y	probably damaging	Het
Vwa2	T	G	19: 56,909,315	L684R	probably damaging	Het
Zfp712	T	C	13: 67,041,332	Q377R	probably benign	Het
Zfp947	C	A	17: 22,145,820	G291V	probably damaging	Het

Other mutations in Gpr158

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00340:Gpr158	APN	2	21368683	missense	probably damaging	1.00
IGL00469:Gpr158	APN	2	21746795	splice site	probably benign
IGL00706:Gpr158	APN	2	21746773	missense	probably damaging	1.00
IGL00780:Gpr158	APN	2	21826818	nonsense	probably null
IGL00885:Gpr158	APN	2	21649021	missense	probably damaging	1.00
IGL01339:Gpr158	APN	2	21369031	missense	possibly damaging	0.73
IGL01368:Gpr158	APN	2	21827098	missense	probably damaging	1.00
IGL02141:Gpr158	APN	2	21783290	missense	probably damaging	0.99
IGL02455:Gpr158	APN	2	21368700	missense	probably benign	0.00
IGL02554:Gpr158	APN	2	21826596	missense	probably benign
IGL02681:Gpr158	APN	2	21815630	missense	probably damaging	1.00
IGL02752:Gpr158	APN	2	21826827	missense	possibly damaging	0.95
IGL02756:Gpr158	APN	2	21827079	missense	possibly damaging	0.47
IGL03181:Gpr158	APN	2	21783161	missense	probably benign	0.02
IGL03258:Gpr158	APN	2	21825274	missense	probably damaging	1.00
IGL03386:Gpr158	APN	2	21826246	missense	probably damaging	1.00
PIT4810001:Gpr158	UTSW	2	21826871	missense	probably benign	0.01
R0071:Gpr158	UTSW	2	21810668	missense	probably benign	0.08
R0081:Gpr158	UTSW	2	21826717	missense	probably damaging	1.00
R0528:Gpr158	UTSW	2	21825208	missense	probably damaging	1.00
R0560:Gpr158	UTSW	2	21825274	missense	probably damaging	1.00
R0603:Gpr158	UTSW	2	21815669	missense	possibly damaging	0.67
R1560:Gpr158	UTSW	2	21826314	missense	probably damaging	1.00
R1561:Gpr158	UTSW	2	21815694	splice site	probably null
R1609:Gpr158	UTSW	2	21783293	missense	possibly damaging	0.61
R1741:Gpr158	UTSW	2	21827548	missense	probably benign	0.00
R1827:Gpr158	UTSW	2	21827318	missense	probably benign
R1854:Gpr158	UTSW	2	21369124	missense	probably damaging	1.00
R1871:Gpr158	UTSW	2	21815615	missense	probably damaging	1.00
R2151:Gpr158	UTSW	2	21827514	missense	possibly damaging	0.82
R2273:Gpr158	UTSW	2	21826863	missense	probably benign
R2275:Gpr158	UTSW	2	21826863	missense	probably benign
R3004:Gpr158	UTSW	2	21826999	missense	probably damaging	0.99
R3151:Gpr158	UTSW	2	21576960	missense	possibly damaging	0.68
R4238:Gpr158	UTSW	2	21368551	missense	probably damaging	1.00
R4379:Gpr158	UTSW	2	21825214	missense	probably damaging	1.00
R4381:Gpr158	UTSW	2	21827592	missense	probably damaging	1.00
R4464:Gpr158	UTSW	2	21826999	missense	probably damaging	0.99
R4467:Gpr158	UTSW	2	21826999	missense	probably damaging	0.99
R4496:Gpr158	UTSW	2	21826999	missense	probably damaging	0.99
R4506:Gpr158	UTSW	2	21826999	missense	probably damaging	0.99
R4530:Gpr158	UTSW	2	21369000	missense	probably benign	0.03
R4646:Gpr158	UTSW	2	21827053	missense	probably benign
R4798:Gpr158	UTSW	2	21783182	missense	probably damaging	1.00
R4882:Gpr158	UTSW	2	21825248	missense	probably damaging	0.98
R4943:Gpr158	UTSW	2	21827157	missense	probably damaging	1.00
R5334:Gpr158	UTSW	2	21827505	missense	probably benign	0.01
R5560:Gpr158	UTSW	2	21826290	missense	possibly damaging	0.67
R5600:Gpr158	UTSW	2	21827235	missense	probably benign
R5637:Gpr158	UTSW	2	21783272	missense	probably benign	0.00
R5701:Gpr158	UTSW	2	21746709	missense	probably damaging	1.00
R5744:Gpr158	UTSW	2	21368520	missense	probably damaging	1.00
R5911:Gpr158	UTSW	2	21369121	missense	possibly damaging	0.95
R5991:Gpr158	UTSW	2	21368508	missense	probably damaging	0.99
R6200:Gpr158	UTSW	2	21399416	missense	probably damaging	0.97
R6306:Gpr158	UTSW	2	21815611	missense	possibly damaging	0.84
R6324:Gpr158	UTSW	2	21810554	missense	probably damaging	1.00
R6384:Gpr158	UTSW	2	21826288	missense	probably damaging	1.00
R6698:Gpr158	UTSW	2	21827110	missense	probably damaging	1.00
R6997:Gpr158	UTSW	2	21648991	missense	possibly damaging	0.46
R7086:Gpr158	UTSW	2	21826575	missense	probably benign	0.01
R7175:Gpr158	UTSW	2	21368302	missense	probably benign	0.13
R7197:Gpr158	UTSW	2	21810601	missense	probably damaging	0.99
R7293:Gpr158	UTSW	2	21576939	missense	possibly damaging	0.47
R7427:Gpr158	UTSW	2	21827318	missense	probably benign
R7515:Gpr158	UTSW	2	21368281	missense	probably damaging	1.00
R7730:Gpr158	UTSW	2	21826347	missense	probably damaging	1.00
R8122:Gpr158	UTSW	2	21826863	missense	probably benign
R8311:Gpr158	UTSW	2	21368890	missense	probably benign	0.00
R8754:Gpr158	UTSW	2	21576882	missense	probably benign	0.00
R8782:Gpr158	UTSW	2	21399338	missense	probably damaging	1.00
R8792:Gpr158	UTSW	2	21553326	missense	probably damaging	1.00
R8842:Gpr158	UTSW	2	21576940	missense	possibly damaging	0.88
R9009:Gpr158	UTSW	2	21576949	missense	probably damaging	1.00
R9102:Gpr158	UTSW	2	21825267	missense	probably damaging	1.00
R9150:Gpr158	UTSW	2	21826440	missense	probably benign	0.17
R9254:Gpr158	UTSW	2	21368231	start gained	probably benign
R9317:Gpr158	UTSW	2	21827226	missense	probably benign
R9379:Gpr158	UTSW	2	21368231	start gained	probably benign
R9428:Gpr158	UTSW	2	21783161	missense	probably benign
R9497:Gpr158	UTSW	2	21827014	missense	probably benign	0.00
R9667:Gpr158	UTSW	2	21825243	missense	probably damaging	0.99
R9681:Gpr158	UTSW	2	21826504	missense	probably damaging	0.99
X0062:Gpr158	UTSW	2	21826369	missense	probably damaging	1.00
Z1176:Gpr158	UTSW	2	21810690	critical splice donor site	probably null
Z1177:Gpr158	UTSW	2	21827272	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- ATGCTAGACAGATGGGAGCC -3'
(R):5'- TGGTACCACTGCAGGTCATC -3'

Sequencing Primer
(F):5'- CTGCTCGCTCATCTGGGATTG -3'
(R):5'- AGGTCATCCTGCACGGTCTG -3'

Posted On

2015-04-17