Incidental Mutation 'R3945:Trim66'
ID 307648
Institutional Source Beutler Lab
Gene Symbol Trim66
Ensembl Gene ENSMUSG00000031026
Gene Name tripartite motif-containing 66
Synonyms Tif1d, D7H11orf29
MMRRC Submission 040926-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.169) question?
Stock # R3945 (G1)
Quality Score 225
Status Validated
Chromosome 7
Chromosomal Location 109048213-109107341 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to A at 109071475 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Isoleucine at position 608 (T608I)
Ref Sequence ENSEMBL: ENSMUSP00000102350 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000033339] [ENSMUST00000106739] [ENSMUST00000106741]
AlphaFold Q924W6
Predicted Effect possibly damaging
Transcript: ENSMUST00000033339
AA Change: T608I

PolyPhen 2 Score 0.940 (Sensitivity: 0.80; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000033339
Gene: ENSMUSG00000031026
AA Change: T608I

DomainStartEndE-ValueType
PHD 4 69 7.77e0 SMART
BBC 108 234 1.61e-39 SMART
low complexity region 318 333 N/A INTRINSIC
low complexity region 452 486 N/A INTRINSIC
low complexity region 517 530 N/A INTRINSIC
low complexity region 568 581 N/A INTRINSIC
PHD 998 1041 4.09e-10 SMART
BROMO 1069 1175 8.22e-27 SMART
low complexity region 1185 1199 N/A INTRINSIC
Predicted Effect possibly damaging
Transcript: ENSMUST00000106739
AA Change: T608I

PolyPhen 2 Score 0.940 (Sensitivity: 0.80; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000102350
Gene: ENSMUSG00000031026
AA Change: T608I

DomainStartEndE-ValueType
PHD 4 69 7.77e0 SMART
BBC 108 234 1.61e-39 SMART
low complexity region 318 333 N/A INTRINSIC
low complexity region 452 486 N/A INTRINSIC
low complexity region 517 530 N/A INTRINSIC
low complexity region 568 581 N/A INTRINSIC
PHD 998 1041 4.09e-10 SMART
BROMO 1069 1175 8.22e-27 SMART
low complexity region 1185 1199 N/A INTRINSIC
Predicted Effect possibly damaging
Transcript: ENSMUST00000106741
AA Change: T710I

PolyPhen 2 Score 0.628 (Sensitivity: 0.87; Specificity: 0.91)
SMART Domains Protein: ENSMUSP00000102352
Gene: ENSMUSG00000031026
AA Change: T710I

DomainStartEndE-ValueType
RING 28 78 2.38e-2 SMART
BBOX 102 140 1.48e0 SMART
PHD 106 171 7.77e0 SMART
RING 107 170 4.38e0 SMART
BBOX 162 203 4.21e-3 SMART
BBC 210 336 1.61e-39 SMART
low complexity region 420 435 N/A INTRINSIC
low complexity region 554 588 N/A INTRINSIC
low complexity region 619 632 N/A INTRINSIC
low complexity region 670 683 N/A INTRINSIC
PHD 1100 1143 4.09e-10 SMART
BROMO 1171 1277 8.22e-27 SMART
low complexity region 1287 1301 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000137704
Meta Mutation Damage Score 0.0724 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.7%
  • 10x: 97.4%
  • 20x: 95.4%
Validation Efficiency 100% (54/54)
Allele List at MGI
Other mutations in this stock
Total: 49 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930407I10Rik T C 15: 81,949,601 (GRCm39) L1166P probably damaging Het
Actn4 T C 7: 28,611,661 (GRCm39) probably null Het
Adamts17 A T 7: 66,770,687 (GRCm39) E905V probably benign Het
Adck5 A G 15: 76,479,400 (GRCm39) N485S probably damaging Het
Agr3 C A 12: 35,997,512 (GRCm39) probably benign Het
Ankrd12 G A 17: 66,283,098 (GRCm39) T1921I probably damaging Het
Ascl2 T C 7: 142,521,708 (GRCm39) S247G probably benign Het
Atp7b T C 8: 22,510,880 (GRCm39) E422G probably benign Het
Bltp2 T C 11: 78,180,790 (GRCm39) I2229T probably damaging Het
C630050I24Rik C T 8: 107,845,894 (GRCm39) R15* probably null Het
Cabin1 T G 10: 75,581,093 (GRCm39) Q411P probably damaging Het
Chrne T C 11: 70,507,869 (GRCm39) I277V possibly damaging Het
Coch A G 12: 51,648,595 (GRCm39) probably null Het
Corin A G 5: 72,515,767 (GRCm39) V429A probably damaging Het
Cpa3 A T 3: 20,279,281 (GRCm39) N219K probably damaging Het
Creb3l1 T C 2: 91,821,556 (GRCm39) E273G probably damaging Het
Csmd1 A C 8: 15,960,619 (GRCm39) probably null Het
Ddx59 A G 1: 136,362,356 (GRCm39) D527G probably damaging Het
Defa25 G A 8: 21,574,506 (GRCm39) V17I probably null Het
Efs A G 14: 55,158,108 (GRCm39) probably benign Het
Ern2 A G 7: 121,775,753 (GRCm39) M447T probably benign Het
Fgfr2 C T 7: 129,779,485 (GRCm39) E596K possibly damaging Het
Filip1 T C 9: 79,725,649 (GRCm39) K990R probably benign Het
Ipo8 T A 6: 148,719,615 (GRCm39) Q110L probably damaging Het
Kank4 T A 4: 98,659,517 (GRCm39) I854F probably damaging Het
Mst1 G A 9: 107,962,052 (GRCm39) C681Y probably damaging Het
Nr2c2 C T 6: 92,140,119 (GRCm39) R464W probably damaging Het
Or1s2 A G 19: 13,758,786 (GRCm39) E270G probably benign Het
Or4f4b C T 2: 111,314,032 (GRCm39) Q86* probably null Het
Pde11a T C 2: 75,906,275 (GRCm39) probably benign Het
Ptprq A G 10: 107,522,253 (GRCm39) probably benign Het
Rcbtb1 G A 14: 59,462,225 (GRCm39) probably null Het
Rpl37 G A 15: 5,147,176 (GRCm39) R72H probably benign Het
Samd9l A T 6: 3,377,029 (GRCm39) S77R possibly damaging Het
Sin3b A G 8: 73,460,067 (GRCm39) D218G possibly damaging Het
Slc22a23 A G 13: 34,367,109 (GRCm39) I633T probably damaging Het
Spen T C 4: 141,204,664 (GRCm39) D1321G unknown Het
Ssh2 T C 11: 77,345,494 (GRCm39) S1160P possibly damaging Het
Synrg T A 11: 83,914,232 (GRCm39) D952E probably damaging Het
Tigd3 A G 19: 5,942,461 (GRCm39) F223S probably damaging Het
Trmt13 A G 3: 116,375,167 (GRCm39) F447S probably damaging Het
Trpc2 T C 7: 101,737,486 (GRCm39) I800T possibly damaging Het
Ugt3a1 A T 15: 9,370,184 (GRCm39) I443F possibly damaging Het
Vamp2 C A 11: 68,980,000 (GRCm39) P24Q unknown Het
Vmn1r113 A G 7: 20,521,637 (GRCm39) Y143C probably benign Het
Vmn1r14 T A 6: 57,211,254 (GRCm39) N277K probably benign Het
Vmn1r181 T A 7: 23,683,577 (GRCm39) V14E probably damaging Het
Wdfy4 A T 14: 32,688,352 (GRCm39) I3086N probably damaging Het
Zfp988 A C 4: 147,417,242 (GRCm39) K559Q probably benign Het
Other mutations in Trim66
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01539:Trim66 APN 7 109,054,273 (GRCm39) missense probably benign 0.02
IGL01758:Trim66 APN 7 109,085,252 (GRCm39) critical splice donor site probably null
IGL01982:Trim66 APN 7 109,057,970 (GRCm39) missense probably benign 0.00
IGL01983:Trim66 APN 7 109,057,458 (GRCm39) nonsense probably null
IGL02149:Trim66 APN 7 109,060,109 (GRCm39) missense possibly damaging 0.66
IGL02392:Trim66 APN 7 109,059,481 (GRCm39) missense probably benign 0.01
IGL02483:Trim66 APN 7 109,076,837 (GRCm39) splice site probably benign
IGL02832:Trim66 APN 7 109,059,704 (GRCm39) missense probably damaging 1.00
IGL02945:Trim66 APN 7 109,059,383 (GRCm39) nonsense probably null
IGL03085:Trim66 APN 7 109,057,952 (GRCm39) missense probably benign 0.17
PIT1430001:Trim66 UTSW 7 109,074,454 (GRCm39) missense probably damaging 0.99
R0326:Trim66 UTSW 7 109,059,379 (GRCm39) missense probably benign 0.00
R0358:Trim66 UTSW 7 109,059,383 (GRCm39) nonsense probably null
R0401:Trim66 UTSW 7 109,074,471 (GRCm39) missense probably damaging 0.98
R0470:Trim66 UTSW 7 109,056,749 (GRCm39) splice site probably benign
R0568:Trim66 UTSW 7 109,059,902 (GRCm39) missense probably benign 0.00
R0669:Trim66 UTSW 7 109,054,199 (GRCm39) intron probably benign
R0980:Trim66 UTSW 7 109,054,877 (GRCm39) missense probably damaging 1.00
R1015:Trim66 UTSW 7 109,054,440 (GRCm39) missense probably damaging 1.00
R1078:Trim66 UTSW 7 109,071,526 (GRCm39) missense probably damaging 1.00
R1099:Trim66 UTSW 7 109,074,661 (GRCm39) missense probably benign 0.34
R1181:Trim66 UTSW 7 109,083,784 (GRCm39) critical splice donor site probably null
R1497:Trim66 UTSW 7 109,083,826 (GRCm39) missense probably benign 0.00
R1583:Trim66 UTSW 7 109,054,287 (GRCm39) missense probably damaging 1.00
R1843:Trim66 UTSW 7 109,075,046 (GRCm39) missense probably damaging 0.99
R1998:Trim66 UTSW 7 109,083,784 (GRCm39) critical splice donor site probably null
R2016:Trim66 UTSW 7 109,071,439 (GRCm39) critical splice donor site probably null
R2143:Trim66 UTSW 7 109,074,320 (GRCm39) missense probably damaging 0.98
R2144:Trim66 UTSW 7 109,074,320 (GRCm39) missense probably damaging 0.98
R2145:Trim66 UTSW 7 109,074,320 (GRCm39) missense probably damaging 0.98
R4012:Trim66 UTSW 7 109,057,338 (GRCm39) missense probably damaging 0.98
R4464:Trim66 UTSW 7 109,076,897 (GRCm39) missense possibly damaging 0.51
R4473:Trim66 UTSW 7 109,081,202 (GRCm39) missense probably damaging 1.00
R4729:Trim66 UTSW 7 109,055,267 (GRCm39) critical splice donor site probably null
R4730:Trim66 UTSW 7 109,082,276 (GRCm39) missense probably damaging 1.00
R4775:Trim66 UTSW 7 109,056,796 (GRCm39) nonsense probably null
R4819:Trim66 UTSW 7 109,056,793 (GRCm39) missense probably damaging 1.00
R5269:Trim66 UTSW 7 109,056,797 (GRCm39) missense probably benign 0.00
R5557:Trim66 UTSW 7 109,082,944 (GRCm39) missense probably benign 0.06
R5832:Trim66 UTSW 7 109,054,409 (GRCm39) missense probably damaging 1.00
R6220:Trim66 UTSW 7 109,082,300 (GRCm39) missense probably damaging 0.97
R6243:Trim66 UTSW 7 109,059,481 (GRCm39) missense probably benign 0.01
R6374:Trim66 UTSW 7 109,085,269 (GRCm39) missense probably benign
R6450:Trim66 UTSW 7 109,059,945 (GRCm39) missense probably benign 0.09
R6543:Trim66 UTSW 7 109,075,086 (GRCm39) missense probably benign 0.01
R6788:Trim66 UTSW 7 109,076,961 (GRCm39) missense probably damaging 1.00
R6842:Trim66 UTSW 7 109,059,983 (GRCm39) missense probably benign 0.00
R7169:Trim66 UTSW 7 109,054,328 (GRCm39) missense probably benign 0.25
R7257:Trim66 UTSW 7 109,059,451 (GRCm39) missense probably damaging 1.00
R7328:Trim66 UTSW 7 109,056,958 (GRCm39) missense probably damaging 0.99
R7616:Trim66 UTSW 7 109,082,956 (GRCm39) missense probably damaging 0.99
R8423:Trim66 UTSW 7 109,074,599 (GRCm39) missense possibly damaging 0.77
R8855:Trim66 UTSW 7 109,081,188 (GRCm39) missense probably damaging 1.00
R9130:Trim66 UTSW 7 109,076,896 (GRCm39) missense possibly damaging 0.90
R9137:Trim66 UTSW 7 109,074,330 (GRCm39) missense probably damaging 0.99
R9640:Trim66 UTSW 7 109,074,825 (GRCm39) missense probably damaging 1.00
RF013:Trim66 UTSW 7 109,059,960 (GRCm39) missense probably damaging 0.99
RF024:Trim66 UTSW 7 109,059,947 (GRCm39) missense possibly damaging 0.62
Predicted Primers PCR Primer
(F):5'- CACTCTAAATGAAGCCCAGTGAG -3'
(R):5'- TGGTATGCTCTCTGGGGAAAC -3'

Sequencing Primer
(F):5'- TGAAGCCCAGTGAGCCCATATG -3'
(R):5'- AAGCCAGCTCTACCTGTT -3'
Posted On 2015-04-17