|Institutional Source||Beutler Lab|
|Gene Name||anterior gradient 3|
|Is this an essential gene?||Probably non essential (E-score: 0.062)|
|Stock #||R3946 (G1)|
|Chromosomal Location||35925620-35949736 bp(+) (GRCm38)|
|Type of Mutation||splice site|
|DNA Base Change (assembly)||C to A at 35947513 bp|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000049212 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000042101]|
|Predicted Effect||probably benign
|Coding Region Coverage||
|Validation Efficiency||98% (60/61)|
|MGI Phenotype||FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, a catalytically active thioredoxin domain, and a C-terminal ER-retention sequence. This gene is expressed in ciliated airway epithelial cells and, in mouse, plays a role in ciliary beat frequency in multiciliated cells. This gene is also over-expressed in breast, ovarian, and prostrate cancers. [provided by RefSeq, Dec 2016]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Agr3||
(F):5'- GTCTGTTGACATCTCCACAAGC -3'
(R):5'- TGGTCTCATGCTAGCAGAAG -3'
(F):5'- TGTTGACATCTCCACAAGCAAACTC -3'
(R):5'- GCATTCTTTATGGAAGCTAAATTTCC -3'