Incidental Mutation 'R3932:Arl1'
ID308506
Institutional Source Beutler Lab
Gene Symbol Arl1
Ensembl Gene ENSMUSG00000060904
Gene NameADP-ribosylation factor-like 1
Synonyms
MMRRC Submission 040919-MU
Accession Numbers
Is this an essential gene? Possibly essential (E-score: 0.731) question?
Stock #R3932 (G1)
Quality Score225
Status Validated
Chromosome10
Chromosomal Location88730858-88744094 bp(+) (GRCm38)
Type of Mutationsplice site
DNA Base Change (assembly) C to T at 88733536 bp
ZygosityHeterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000127889 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000080379] [ENSMUST00000116234] [ENSMUST00000170137]
Predicted Effect probably benign
Transcript: ENSMUST00000080379
SMART Domains Protein: ENSMUSP00000079246
Gene: ENSMUSG00000060904

DomainStartEndE-ValueType
ARF 1 138 1.23e-55 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000092231
Predicted Effect probably benign
Transcript: ENSMUST00000116234
SMART Domains Protein: ENSMUSP00000111942
Gene: ENSMUSG00000060904

DomainStartEndE-ValueType
ARF 1 181 2.44e-91 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000168843
Predicted Effect probably benign
Transcript: ENSMUST00000170137
SMART Domains Protein: ENSMUSP00000127889
Gene: ENSMUSG00000060904

DomainStartEndE-ValueType
Pfam:Arf 5 52 4.6e-19 PFAM
Coding Region Coverage
  • 1x: 99.4%
  • 3x: 98.7%
  • 10x: 97.1%
  • 20x: 94.1%
Validation Efficiency 100% (52/52)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
Allele List at MGI
Other mutations in this stock
Total: 48 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ap3b2 C T 7: 81,473,850 probably benign Het
Arsi G A 18: 60,916,651 G202E probably benign Het
Atp10a T C 7: 58,827,104 M1189T possibly damaging Het
BC051142 A G 17: 34,443,443 T86A possibly damaging Het
Bcl2l14 A G 6: 134,423,808 D64G probably damaging Het
Cdc34b G A 11: 94,742,615 V214M probably benign Het
Cfap54 T C 10: 92,829,757 T2985A probably benign Het
Clcc1 T C 3: 108,673,366 M332T probably damaging Het
Coch T C 12: 51,603,338 I370T probably damaging Het
Ctdnep1 A G 11: 69,989,574 probably benign Het
Edar C T 10: 58,610,342 C221Y probably damaging Het
Fam135b T A 15: 71,450,431 Q1295L probably benign Het
Fam184a C T 10: 53,699,301 A71T probably damaging Het
Fbxw10 A G 11: 62,869,157 probably benign Het
Frmd4a T C 2: 4,537,260 W247R probably damaging Het
Gcn1l1 A G 5: 115,587,834 H553R probably benign Het
Grin3a C T 4: 49,672,472 probably null Het
H2-Q6 C T 17: 35,425,566 probably benign Het
Hivep2 C A 10: 14,128,969 T437K probably benign Het
Hspg2 T C 4: 137,515,568 V670A probably damaging Het
Med10 A G 13: 69,809,982 N18D probably damaging Het
Mgat4b T A 11: 50,233,338 H368Q possibly damaging Het
Morn5 C A 2: 36,053,023 T45N probably damaging Het
Mrc2 G A 11: 105,348,431 probably null Het
Msl3 C T X: 168,671,817 A87T probably damaging Het
Myrf T C 19: 10,218,151 T432A probably damaging Het
Nav3 T A 10: 109,694,035 E2148D probably damaging Het
Nfe2l3 A G 6: 51,456,615 T236A possibly damaging Het
Odc1 T A 12: 17,548,800 F227Y probably benign Het
Olfr1444 T C 19: 12,862,630 M285T possibly damaging Het
Opa1 A T 16: 29,610,880 E401D probably damaging Het
Pdcd1 T C 1: 94,041,264 I110V probably benign Het
Pde5a G A 3: 122,760,896 E212K probably damaging Het
Plin4 A G 17: 56,106,704 I307T probably benign Het
Rag1 T C 2: 101,643,039 Y586C probably damaging Het
Rgs7bp T C 13: 105,052,998 M98V probably benign Het
Rgs9 A G 11: 109,275,813 probably benign Het
Rin3 A G 12: 102,390,083 D961G probably damaging Het
Rubcn A G 16: 32,829,259 probably null Het
Slc13a2 T A 11: 78,398,400 Y495F probably damaging Het
Tfec T A 6: 16,845,459 D67V probably damaging Het
Tmem28 G A X: 99,821,864 V266M possibly damaging Het
Tmem94 T C 11: 115,789,254 M30T probably benign Het
Tubgcp6 A G 15: 89,104,414 probably benign Het
Vmn2r10 C A 5: 109,002,222 A319S possibly damaging Het
Vmn2r85 T C 10: 130,418,467 M783V probably damaging Het
Zfp422 T C 6: 116,626,459 K193R probably benign Het
Zfp94 T G 7: 24,303,687 D110A probably benign Het
Other mutations in Arl1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01670:Arl1 APN 10 88742022 missense probably damaging 1.00
IGL03181:Arl1 APN 10 88743059 utr 3 prime probably benign
R1851:Arl1 UTSW 10 88733546 utr 5 prime probably benign
R4597:Arl1 UTSW 10 88730746 unclassified probably benign
R4700:Arl1 UTSW 10 88730637 unclassified probably benign
R5419:Arl1 UTSW 10 88737104 missense probably damaging 1.00
R5875:Arl1 UTSW 10 88741979 missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- TGGGAACTTCACGTGTTACTG -3'
(R):5'- CCCATCATATGACTCGTTTATTCAG -3'

Sequencing Primer
(F):5'- GAACTTCACGTGTTACTGTTTGC -3'
(R):5'- TGACTCGTTTATTCAGAAAGCTG -3'
Posted On2015-04-17