Mutagenetix > Incidental Mutation

Incidental Mutation 'R3901:Gm5444'

308990

Institutional Source

Beutler Lab

Gene Symbol

Gm5444

Ensembl Gene

ENSMUSG00000053499

Gene Name

predicted gene 5444

Synonyms

MMRRC Submission

040810-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.077) question?

Stock #

R3901 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

4821650-4886484 bp(+) (GRCm39)

Type of Mutation

exon

DNA Base Change (assembly)

T to C at 4884278 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s):

AlphaFold

no structure available at present

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000065956

SMART Domains

Protein: ENSMUSP00000070493
Gene: ENSMUSG00000053499

Domain	Start	End	E-Value	Type
transmembrane domain	7	29	N/A	INTRINSIC
low complexity region	83	103	N/A	INTRINSIC

Meta Mutation Damage Score

0.0869

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.7%

Validation Efficiency

98% (39/40)

Allele List at MGI

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts20	T	A	15: 94,226,726 (GRCm39)	D1109V	possibly damaging	Het
Apc2	G	C	10: 80,150,922 (GRCm39)	R1992P	possibly damaging	Het
Atmin	A	G	8: 117,683,036 (GRCm39)	N232S	probably benign	Het
Brat1	A	C	5: 140,703,751 (GRCm39)	D668A	possibly damaging	Het
Casp16	A	T	17: 23,771,922 (GRCm39)	V101E	probably damaging	Het
Cass4	C	T	2: 172,274,478 (GRCm39)	P753L	probably damaging	Het
Cimip3	AC	A	17: 47,744,348 (GRCm39)		probably benign	Het
Clec7a	A	G	6: 129,445,877 (GRCm39)	S98P	possibly damaging	Het
Cpne5	G	A	17: 29,378,082 (GRCm39)	R566C	unknown	Het
Csrnp1	C	T	9: 119,801,707 (GRCm39)	E451K	probably damaging	Het
Dlst	A	G	12: 85,179,465 (GRCm39)	T435A	possibly damaging	Het
Dock8	A	G	19: 25,078,269 (GRCm39)	T525A	possibly damaging	Het
Dync2li1	A	G	17: 84,939,070 (GRCm39)	T45A	probably damaging	Het
Efcab3	A	G	11: 104,974,713 (GRCm39)	N5307S	possibly damaging	Het
Epha4	A	G	1: 77,357,539 (GRCm39)	Y820H	probably damaging	Het
F5	A	G	1: 164,003,798 (GRCm39)	T198A	probably benign	Het
Fbn2	T	A	18: 58,199,083 (GRCm39)	N1395I	probably damaging	Het
Fcnb	T	A	2: 27,969,208 (GRCm39)	Y163F	probably damaging	Het
Jph3	G	T	8: 122,480,158 (GRCm39)	D279Y	possibly damaging	Het
Kcnj3	A	G	2: 55,327,360 (GRCm39)	N50D	possibly damaging	Het
Kcnma1	A	G	14: 23,555,323 (GRCm39)	I416T	probably damaging	Het
Kirrel1	C	T	3: 86,996,458 (GRCm39)	M380I	probably null	Het
Kmt2e	A	G	5: 23,706,640 (GRCm39)	N1401S	probably benign	Het
Lama5	C	T	2: 179,824,144 (GRCm39)		probably benign	Het
Lrp1b	A	G	2: 40,712,707 (GRCm39)	V3095A	probably damaging	Het
Mmp1a	A	G	9: 7,475,346 (GRCm39)	*372W	probably null	Het
Or7d11	C	T	9: 19,966,169 (GRCm39)	V197I	probably benign	Het
Pdgfra	T	A	5: 75,353,169 (GRCm39)	N986K	probably benign	Het
Pgap1	C	T	1: 54,532,507 (GRCm39)	V671I	probably benign	Het
Pla2g4e	T	C	2: 119,999,085 (GRCm39)	S760G	probably benign	Het
Plk3	T	C	4: 116,990,633 (GRCm39)	I94V	probably benign	Het
Pogk	C	T	1: 166,231,193 (GRCm39)	V45I	probably damaging	Het
Pou2f1	G	C	1: 165,722,538 (GRCm39)	P349R	probably damaging	Het
Rims1	T	A	1: 22,572,578 (GRCm39)	Q541L	probably benign	Het
Rptn	A	G	3: 93,305,664 (GRCm39)	Q999R	probably benign	Het
Stxbp5	A	G	10: 9,645,163 (GRCm39)	L911P	probably damaging	Het
Tgfbr3	A	G	5: 107,362,753 (GRCm39)		probably benign	Het
Trim7	A	T	11: 48,728,435 (GRCm39)	T28S	probably damaging	Het
Zfp595	T	C	13: 67,465,379 (GRCm39)	I295V	probably benign	Het

Other mutations in Gm5444

Allele	Source	Chr	Coord	Type	Predicted Effect
R0716:Gm5444	UTSW	13	4,884,192 (GRCm39)	exon	noncoding transcript
R2332:Gm5444	UTSW	13	4,883,624 (GRCm39)	exon	noncoding transcript
R2341:Gm5444	UTSW	13	4,884,344 (GRCm39)	exon	noncoding transcript
R4520:Gm5444	UTSW	13	4,884,225 (GRCm39)	exon	noncoding transcript
R5148:Gm5444	UTSW	13	4,884,314 (GRCm39)	exon	noncoding transcript
R5856:Gm5444	UTSW	13	4,821,683 (GRCm39)	exon	noncoding transcript

Predicted Primers

PCR Primer
(F):5'- CACTAGCTTCAGCCCATAGG -3'
(R):5'- TGAACCTGAAGACCTGAATTCAAC -3'

Sequencing Primer
(F):5'- ATGGTTGTAAGCCACCATGC -3'
(R):5'- GCTTATAACCCTTGTGCCAGAAAG -3'

Posted On

2015-04-17