Mutagenetix > Incidental Mutation

Incidental Mutation 'R3902:Taar4'

309242

Institutional Source

Beutler Lab

Gene Symbol

Taar4

Ensembl Gene

ENSMUSG00000069707

Gene Name

trace amine-associated receptor 4

Synonyms

MMRRC Submission

040811-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.122) question?

Stock #

R3902 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

23836392-23837435 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 23836913 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 174 (N174K)

Ref Sequence

ENSEMBL: ENSMUSP00000090330 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000092660]

AlphaFold

Q5QD15

Predicted Effect

probably damaging
Transcript: ENSMUST00000092660
AA Change: N174K

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000090330
Gene: ENSMUSG00000069707
AA Change: N174K

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	44	328	9.2e-11	PFAM
Pfam:7tm_1	50	313	4.6e-63	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.0%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit impaired olfactory response and aversion to PEA and puma urine. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Atmin	A	G	8: 117,683,036 (GRCm39)	N232S	probably benign	Het
Brat1	A	C	5: 140,703,751 (GRCm39)	D668A	possibly damaging	Het
Eif2b3	C	T	4: 116,879,404 (GRCm39)	R15W	probably damaging	Het
Eprs1	G	A	1: 185,111,939 (GRCm39)		probably null	Het
F5	A	G	1: 164,003,798 (GRCm39)	T198A	probably benign	Het
Fbxl20	T	A	11: 97,987,861 (GRCm39)	T61S	probably benign	Het
Fry	A	G	5: 150,269,392 (GRCm39)	E211G	probably damaging	Het
Gatd1	A	G	7: 140,989,014 (GRCm39)	L215P	probably damaging	Het
Gys2	A	G	6: 142,418,526 (GRCm39)	M1T	probably null	Het
Hacd4	A	C	4: 88,355,738 (GRCm39)	I49R	probably damaging	Het
Jph3	G	T	8: 122,480,158 (GRCm39)	D279Y	possibly damaging	Het
Klhl26	T	C	8: 70,905,016 (GRCm39)	D217G	probably damaging	Het
Kmt2e	A	G	5: 23,706,640 (GRCm39)	N1401S	probably benign	Het
Mettl21a	C	T	1: 64,647,240 (GRCm39)	V106I	probably benign	Het
Mpdz	A	G	4: 81,225,353 (GRCm39)	V1427A	probably damaging	Het
Mug2	A	T	6: 122,052,526 (GRCm39)	D1024V	probably damaging	Het
Myl7	T	A	11: 5,848,430 (GRCm39)	K38M	probably damaging	Het
Myl7	T	G	11: 5,848,431 (GRCm39)	K38Q	probably damaging	Het
Myom2	T	C	8: 15,154,165 (GRCm39)	V701A	probably benign	Het
Nipbl	A	G	15: 8,379,730 (GRCm39)	S1021P	possibly damaging	Het
Optc	A	G	1: 133,825,701 (GRCm39)	M275T	probably benign	Het
Pclo	A	T	5: 14,762,536 (GRCm39)	T385S	probably benign	Het
Pdgfra	T	A	5: 75,353,169 (GRCm39)	N986K	probably benign	Het
Pira1	G	A	7: 3,740,276 (GRCm39)	T315I	probably damaging	Het
Plekhn1	T	A	4: 156,310,126 (GRCm39)	I63F	possibly damaging	Het
Pogk	C	T	1: 166,231,193 (GRCm39)	V45I	probably damaging	Het
Rassf1	A	G	9: 107,432,039 (GRCm39)	Y21C	probably damaging	Het
Slc12a1	A	T	2: 125,030,113 (GRCm39)	I562F	probably damaging	Het
Slc16a6	A	G	11: 109,349,387 (GRCm39)	S141P	probably damaging	Het
Sntn	T	C	14: 13,679,084 (GRCm38)	L86P	probably damaging	Het
Styx-ps	A	G	X: 67,865,253 (GRCm39)	M101V	probably benign	Het
Trav12-3	G	T	14: 53,859,486 (GRCm39)	C44F	probably damaging	Het
Triml2	A	G	8: 43,643,397 (GRCm39)	R240G	probably benign	Het
Vmn1r3	A	G	4: 3,185,241 (GRCm39)	M22T	probably benign	Het
Vmn2r7	C	A	3: 64,626,937 (GRCm39)	Q26H	possibly damaging	Het
Vmn2r72	T	C	7: 85,398,943 (GRCm39)	D470G	possibly damaging	Het
Xpo6	A	G	7: 125,719,581 (GRCm39)	Y602H	probably damaging	Het
Zfp287	T	C	11: 62,603,028 (GRCm39)	T241A	probably benign	Het
Zfp386	A	G	12: 116,023,775 (GRCm39)	K498E	probably damaging	Het
Zzef1	T	C	11: 72,799,326 (GRCm39)	L2392P	probably damaging	Het

Other mutations in Taar4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02576:Taar4	APN	10	23,836,909 (GRCm39)	missense	probably damaging	1.00
IGL03202:Taar4	APN	10	23,836,692 (GRCm39)	missense	probably damaging	1.00
I2288:Taar4	UTSW	10	23,836,818 (GRCm39)	missense	probably benign	0.03
R0103:Taar4	UTSW	10	23,837,304 (GRCm39)	missense	probably damaging	1.00
R0103:Taar4	UTSW	10	23,837,304 (GRCm39)	missense	probably damaging	1.00
R0514:Taar4	UTSW	10	23,836,780 (GRCm39)	missense	probably damaging	1.00
R1222:Taar4	UTSW	10	23,837,230 (GRCm39)	missense	probably benign	0.05
R1248:Taar4	UTSW	10	23,836,936 (GRCm39)	missense	possibly damaging	0.95
R1514:Taar4	UTSW	10	23,836,510 (GRCm39)	missense	possibly damaging	0.71
R1921:Taar4	UTSW	10	23,837,239 (GRCm39)	missense	probably damaging	1.00
R2074:Taar4	UTSW	10	23,837,071 (GRCm39)	missense	probably benign	0.18
R2354:Taar4	UTSW	10	23,836,912 (GRCm39)	missense	probably damaging	1.00
R2392:Taar4	UTSW	10	23,837,172 (GRCm39)	missense	possibly damaging	0.94
R2698:Taar4	UTSW	10	23,837,328 (GRCm39)	missense	probably damaging	1.00
R4688:Taar4	UTSW	10	23,836,731 (GRCm39)	missense	probably damaging	1.00
R5495:Taar4	UTSW	10	23,837,181 (GRCm39)	missense	possibly damaging	0.95
R5595:Taar4	UTSW	10	23,836,639 (GRCm39)	missense	probably damaging	1.00
R5773:Taar4	UTSW	10	23,837,056 (GRCm39)	missense	probably damaging	1.00
R7403:Taar4	UTSW	10	23,836,957 (GRCm39)	missense	probably damaging	1.00
R7581:Taar4	UTSW	10	23,837,052 (GRCm39)	missense	probably damaging	0.97
R7736:Taar4	UTSW	10	23,836,897 (GRCm39)	missense	probably damaging	1.00
R7859:Taar4	UTSW	10	23,837,032 (GRCm39)	missense	probably benign	0.35
R8676:Taar4	UTSW	10	23,836,801 (GRCm39)	missense	possibly damaging	0.56

Predicted Primers

PCR Primer
(F):5'- TGCAAAGTTCACAGCTGCTG -3'
(R):5'- TTGTCCTAGGACCTGTACCAATC -3'

Sequencing Primer
(F):5'- ACAGCTGCTGTGACATCATG -3'
(R):5'- AGGACCTGTACCAATCTGCCTG -3'

Posted On

2015-04-17