Incidental Mutation 'R3911:Tnfrsf11b'
ID 309414
Institutional Source Beutler Lab
Gene Symbol Tnfrsf11b
Ensembl Gene ENSMUSG00000063727
Gene Name tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
Synonyms OPG, OCIF, TR1, osteoclastogenesis inhibitory factor, Opg
MMRRC Submission 040909-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.133) question?
Stock # R3911 (G1)
Quality Score 225
Status Validated
Chromosome 15
Chromosomal Location 54114014-54141700 bp(-) (GRCm39)
Type of Mutation splice site
DNA Base Change (assembly) G to A at 54119578 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000078705 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000079772]
AlphaFold O08712
PDB Structure Crystal structure of mouse RANKL-OPG complex [X-RAY DIFFRACTION]
Predicted Effect probably benign
Transcript: ENSMUST00000079772
SMART Domains Protein: ENSMUSP00000078705
Gene: ENSMUSG00000063727

DomainStartEndE-ValueType
signal peptide 1 21 N/A INTRINSIC
TNFR 24 62 1.04e-2 SMART
TNFR 65 105 1.5e-8 SMART
TNFR 107 142 2.19e-10 SMART
TNFR 145 185 7.63e-1 SMART
DEATH 270 365 1.01e-9 SMART
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.3%
  • 20x: 95.0%
Validation Efficiency 100% (71/71)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygote null mice have abnormal bone remodeling that results in severe osteoperosis with increased risk of fractures and growth retardation. Progressive hearing loss also results due to abnormal remodeling of the otic capsule. [provided by MGI curators]
Allele List at MGI

All alleles(4) : Targeted(4)

Other mutations in this stock
Total: 69 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abcb9 T A 5: 124,227,909 (GRCm39) I111F probably benign Het
Abcc12 A C 8: 87,255,048 (GRCm39) probably benign Het
Adgre1 G T 17: 57,754,860 (GRCm39) V653L probably damaging Het
Aebp2 T A 6: 140,593,707 (GRCm39) D230E probably damaging Het
Asap3 A G 4: 135,956,768 (GRCm39) probably benign Het
Bod1l T C 5: 41,974,441 (GRCm39) E2291G probably damaging Het
Casp8 A T 1: 58,872,864 (GRCm39) S267C probably damaging Het
Cchcr1 T C 17: 35,836,233 (GRCm39) V341A probably damaging Het
Cd2ap T A 17: 43,126,980 (GRCm39) probably null Het
Cimip3 AC A 17: 47,744,348 (GRCm39) probably benign Het
Clip1 T A 5: 123,728,897 (GRCm39) E1155D probably damaging Het
Cnnm2 A G 19: 46,866,375 (GRCm39) E841G probably damaging Het
Cntrl G A 2: 35,010,061 (GRCm39) R245H probably damaging Het
Cyp2b23 A T 7: 26,380,842 (GRCm39) S128T probably benign Het
Dhrs11 A T 11: 84,712,579 (GRCm39) I196N probably damaging Het
Dnah17 C T 11: 117,971,675 (GRCm39) probably benign Het
Edc3 A T 9: 57,655,686 (GRCm39) I479F possibly damaging Het
Emg1 T A 6: 124,682,009 (GRCm39) M172L probably benign Het
Epha7 G A 4: 28,938,680 (GRCm39) V512I probably benign Het
Exoc7 T C 11: 116,197,731 (GRCm39) D27G probably benign Het
Fasl C A 1: 161,615,760 (GRCm39) C32F probably benign Het
Flg A T 3: 93,187,307 (GRCm39) H253L probably benign Het
Fnip2 A T 3: 79,386,812 (GRCm39) D971E possibly damaging Het
Gab2 A G 7: 96,948,280 (GRCm39) Y290C probably damaging Het
Gm17067 T A 7: 42,360,104 (GRCm39) I43L possibly damaging Het
Gpld1 A G 13: 25,146,305 (GRCm39) Y183C probably damaging Het
Gpr137c C A 14: 45,516,392 (GRCm39) P327T probably benign Het
Hepacam2 A G 6: 3,494,477 (GRCm39) M1T probably null Het
Ighv5-4 A T 12: 113,561,060 (GRCm39) probably benign Het
Iqca1l A T 5: 24,750,440 (GRCm39) probably benign Het
Itgb8 T A 12: 119,131,740 (GRCm39) E635V possibly damaging Het
Kat14 T C 2: 144,245,982 (GRCm39) V469A probably damaging Het
Kcnj15 C T 16: 95,097,329 (GRCm39) T317I probably damaging Het
Kirrel1 C T 3: 86,996,458 (GRCm39) M380I probably null Het
Klhl18 A T 9: 110,265,151 (GRCm39) L285Q probably damaging Het
Krt90 G A 15: 101,471,218 (GRCm39) R15W probably damaging Het
Krtap4-8 A T 11: 99,670,863 (GRCm39) C203S unknown Het
Lsp1 C T 7: 142,040,098 (GRCm39) S75F probably damaging Het
Map1b A G 13: 99,567,580 (GRCm39) S1714P unknown Het
Mcm2 A G 6: 88,865,234 (GRCm39) I481T probably damaging Het
Mcpt9 T C 14: 56,265,136 (GRCm39) T122A probably benign Het
Megf10 A T 18: 57,422,465 (GRCm39) R947W probably damaging Het
Or2ag18 A T 7: 106,405,072 (GRCm39) V199D probably damaging Het
Or51ai2 A T 7: 103,586,616 (GRCm39) N10Y possibly damaging Het
Pank4 A G 4: 155,054,058 (GRCm39) Y79C probably damaging Het
Pds5b T A 5: 150,670,171 (GRCm39) N386K probably benign Het
Pfkm A G 15: 98,022,928 (GRCm39) M362V probably benign Het
Phc2 T C 4: 128,637,351 (GRCm39) probably null Het
Pou4f1 C T 14: 104,703,611 (GRCm39) A274T unknown Het
Proc A G 18: 32,256,758 (GRCm39) L303P probably damaging Het
Ptk2b C T 14: 66,394,517 (GRCm39) G821D possibly damaging Het
Rlim T C X: 103,006,267 (GRCm39) T545A probably benign Het
Serpinb6c T C 13: 34,077,888 (GRCm39) N161D probably benign Het
Sf3b1 G A 1: 55,058,548 (GRCm39) Q14* probably null Het
Sh3d19 G A 3: 86,014,534 (GRCm39) V442M possibly damaging Het
Slc30a8 A G 15: 52,185,097 (GRCm39) I140V probably benign Het
Spata1 T A 3: 146,181,079 (GRCm39) N293I probably damaging Het
Strap T G 6: 137,712,380 (GRCm39) C10G probably damaging Het
Tcf7 C A 11: 52,173,793 (GRCm39) probably benign Het
Tmem114 A T 16: 8,230,054 (GRCm39) M116K probably damaging Het
Tmem63b A G 17: 45,988,884 (GRCm39) S113P probably damaging Het
Tmtc3 A C 10: 100,284,888 (GRCm39) N582K probably damaging Het
Tns2 T C 15: 102,022,272 (GRCm39) probably null Het
Trim30a A G 7: 104,060,348 (GRCm39) V476A probably damaging Het
Ush2a T A 1: 188,132,151 (GRCm39) V791D probably benign Het
Vmn2r99 T C 17: 19,614,635 (GRCm39) F785S possibly damaging Het
Vps35l A G 7: 118,345,613 (GRCm39) T49A possibly damaging Het
Wdr35 A T 12: 9,036,077 (GRCm39) I283F probably benign Het
Wnt10b G T 15: 98,672,219 (GRCm39) A166E possibly damaging Het
Other mutations in Tnfrsf11b
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00094:Tnfrsf11b APN 15 54,123,238 (GRCm39) missense probably damaging 1.00
IGL00770:Tnfrsf11b APN 15 54,117,468 (GRCm39) missense probably benign 0.16
IGL00774:Tnfrsf11b APN 15 54,117,468 (GRCm39) missense probably benign 0.16
IGL02355:Tnfrsf11b APN 15 54,115,778 (GRCm39) missense probably damaging 0.96
IGL02362:Tnfrsf11b APN 15 54,115,778 (GRCm39) missense probably damaging 0.96
IGL02711:Tnfrsf11b APN 15 54,119,532 (GRCm39) missense probably benign 0.01
IGL02870:Tnfrsf11b APN 15 54,119,423 (GRCm39) missense probably benign 0.05
IGL03219:Tnfrsf11b APN 15 54,117,574 (GRCm39) nonsense probably null
P0012:Tnfrsf11b UTSW 15 54,123,194 (GRCm39) splice site probably benign
R1550:Tnfrsf11b UTSW 15 54,117,454 (GRCm39) missense possibly damaging 0.94
R1813:Tnfrsf11b UTSW 15 54,119,493 (GRCm39) nonsense probably null
R3840:Tnfrsf11b UTSW 15 54,115,478 (GRCm39) missense probably damaging 0.99
R3910:Tnfrsf11b UTSW 15 54,119,578 (GRCm39) splice site probably benign
R3912:Tnfrsf11b UTSW 15 54,119,578 (GRCm39) splice site probably benign
R4299:Tnfrsf11b UTSW 15 54,115,491 (GRCm39) missense probably benign
R4362:Tnfrsf11b UTSW 15 54,119,555 (GRCm39) missense possibly damaging 0.94
R4363:Tnfrsf11b UTSW 15 54,119,555 (GRCm39) missense possibly damaging 0.94
R5288:Tnfrsf11b UTSW 15 54,141,622 (GRCm39) missense probably benign 0.00
R5653:Tnfrsf11b UTSW 15 54,123,262 (GRCm39) missense probably damaging 1.00
R5753:Tnfrsf11b UTSW 15 54,117,455 (GRCm39) missense possibly damaging 0.90
R6881:Tnfrsf11b UTSW 15 54,117,539 (GRCm39) missense probably benign 0.00
R6997:Tnfrsf11b UTSW 15 54,115,770 (GRCm39) missense probably damaging 0.99
R7704:Tnfrsf11b UTSW 15 54,123,497 (GRCm39) missense probably benign 0.30
R7730:Tnfrsf11b UTSW 15 54,117,470 (GRCm39) nonsense probably null
R8017:Tnfrsf11b UTSW 15 54,117,598 (GRCm39) nonsense probably null
R8052:Tnfrsf11b UTSW 15 54,115,502 (GRCm39) missense probably damaging 1.00
R8060:Tnfrsf11b UTSW 15 54,117,505 (GRCm39) missense probably benign 0.38
R8711:Tnfrsf11b UTSW 15 54,123,508 (GRCm39) missense possibly damaging 0.81
R9224:Tnfrsf11b UTSW 15 54,115,556 (GRCm39) missense possibly damaging 0.67
X0025:Tnfrsf11b UTSW 15 54,141,631 (GRCm39) missense probably benign 0.22
Predicted Primers PCR Primer
(F):5'- CTGTTTCCGGAACACACGTTG -3'
(R):5'- ATAGCGCAACAAACTTGTGTGG -3'

Sequencing Primer
(F):5'- TCCGGAACACACGTTGTCATG -3'
(R):5'- CGCAACAAACTTGTGTGGGTATTAC -3'
Posted On 2015-04-17