Mutagenetix > Incidental Mutation

Incidental Mutation 'R3911:Vmn2r99'

309421

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r99

Ensembl Gene

ENSMUSG00000090304

Gene Name

vomeronasal 2, receptor 99

Synonyms

EG665376

MMRRC Submission

040909-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.131) question?

Stock #

R3911 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

19361949-19401098 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 19394373 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 785 (F785S)

Ref Sequence

ENSEMBL: ENSMUSP00000135236 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000176107] [ENSMUST00000231989]

AlphaFold

H3BK37

Predicted Effect

possibly damaging
Transcript: ENSMUST00000176107
AA Change: F785S

PolyPhen 2 Score 0.921 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000135236
Gene: ENSMUSG00000090304
AA Change: F785S

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	81	448	5.7e-33	PFAM
Pfam:NCD3G	508	561	1.8e-21	PFAM
Pfam:7tm_3	593	829	4.6e-52	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000231989

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.0%

Validation Efficiency

100% (71/71)

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700001C19Rik	AC	A	17: 47,433,423 (GRCm38)		probably benign	Het
4931409K22Rik	A	T	5: 24,545,442 (GRCm38)		probably benign	Het
9030624J02Rik	A	G	7: 118,746,390 (GRCm38)	T49A	possibly damaging	Het
Abcb9	T	A	5: 124,089,846 (GRCm38)	I111F	probably benign	Het
Abcc12	A	C	8: 86,528,419 (GRCm38)		probably benign	Het
Adgre1	G	T	17: 57,447,860 (GRCm38)	V653L	probably damaging	Het
Aebp2	T	A	6: 140,647,981 (GRCm38)	D230E	probably damaging	Het
Asap3	A	G	4: 136,229,457 (GRCm38)		probably benign	Het
Bod1l	T	C	5: 41,817,098 (GRCm38)	E2291G	probably damaging	Het
Casp8	A	T	1: 58,833,705 (GRCm38)	S267C	probably damaging	Het
Cchcr1	T	C	17: 35,525,336 (GRCm38)	V341A	probably damaging	Het
Cd2ap	T	A	17: 42,816,089 (GRCm38)		probably null	Het
Clip1	T	A	5: 123,590,834 (GRCm38)	E1155D	probably damaging	Het
Cnnm2	A	G	19: 46,877,936 (GRCm38)	E841G	probably damaging	Het
Cntrl	G	A	2: 35,120,049 (GRCm38)	R245H	probably damaging	Het
Cyp2b23	A	T	7: 26,681,417 (GRCm38)	S128T	probably benign	Het
Dhrs11	A	T	11: 84,821,753 (GRCm38)	I196N	probably damaging	Het
Dnah17	C	T	11: 118,080,849 (GRCm38)		probably benign	Het
Edc3	A	T	9: 57,748,403 (GRCm38)	I479F	possibly damaging	Het
Emg1	T	A	6: 124,705,046 (GRCm38)	M172L	probably benign	Het
Epha7	G	A	4: 28,938,680 (GRCm38)	V512I	probably benign	Het
Exoc7	T	C	11: 116,306,905 (GRCm38)	D27G	probably benign	Het
Fasl	C	A	1: 161,788,191 (GRCm38)	C32F	probably benign	Het
Flg	A	T	3: 93,280,000 (GRCm38)	H253L	probably benign	Het
Fnip2	A	T	3: 79,479,505 (GRCm38)	D971E	possibly damaging	Het
Gab2	A	G	7: 97,299,073 (GRCm38)	Y290C	probably damaging	Het
Gm17067	T	A	7: 42,710,680 (GRCm38)	I43L	possibly damaging	Het
Gpld1	A	G	13: 24,962,322 (GRCm38)	Y183C	probably damaging	Het
Gpr137c	C	A	14: 45,278,935 (GRCm38)	P327T	probably benign	Het
Hepacam2	A	G	6: 3,494,477 (GRCm38)	M1T	probably null	Het
Ighv5-4	A	T	12: 113,597,440 (GRCm38)		probably benign	Het
Itgb8	T	A	12: 119,168,005 (GRCm38)	E635V	possibly damaging	Het
Kat14	T	C	2: 144,404,062 (GRCm38)	V469A	probably damaging	Het
Kcnj15	C	T	16: 95,296,470 (GRCm38)	T317I	probably damaging	Het
Kirrel	C	T	3: 87,089,151 (GRCm38)	M380I	probably null	Het
Klhl18	A	T	9: 110,436,083 (GRCm38)	L285Q	probably damaging	Het
Krt90	G	A	15: 101,562,783 (GRCm38)	R15W	probably damaging	Het
Krtap4-8	A	T	11: 99,780,037 (GRCm38)	C203S	unknown	Het
Lsp1	C	T	7: 142,486,361 (GRCm38)	S75F	probably damaging	Het
Map1b	A	G	13: 99,431,072 (GRCm38)	S1714P	unknown	Het
Mcm2	A	G	6: 88,888,252 (GRCm38)	I481T	probably damaging	Het
Mcpt9	T	C	14: 56,027,679 (GRCm38)	T122A	probably benign	Het
Megf10	A	T	18: 57,289,393 (GRCm38)	R947W	probably damaging	Het
Olfr632	A	T	7: 103,937,409 (GRCm38)	N10Y	possibly damaging	Het
Olfr700	A	T	7: 106,805,865 (GRCm38)	V199D	probably damaging	Het
Pank4	A	G	4: 154,969,601 (GRCm38)	Y79C	probably damaging	Het
Pds5b	T	A	5: 150,746,706 (GRCm38)	N386K	probably benign	Het
Pfkm	A	G	15: 98,125,047 (GRCm38)	M362V	probably benign	Het
Phc2	T	C	4: 128,743,558 (GRCm38)		probably null	Het
Pou4f1	C	T	14: 104,466,175 (GRCm38)	A274T	unknown	Het
Proc	A	G	18: 32,123,705 (GRCm38)	L303P	probably damaging	Het
Ptk2b	C	T	14: 66,157,068 (GRCm38)	G821D	possibly damaging	Het
Rlim	T	C	X: 103,962,661 (GRCm38)	T545A	probably benign	Het
Serpinb6c	T	C	13: 33,893,905 (GRCm38)	N161D	probably benign	Het
Sf3b1	G	A	1: 55,019,389 (GRCm38)	Q14*	probably null	Het
Sh3d19	G	A	3: 86,107,227 (GRCm38)	V442M	possibly damaging	Het
Slc30a8	A	G	15: 52,321,701 (GRCm38)	I140V	probably benign	Het
Spata1	T	A	3: 146,475,324 (GRCm38)	N293I	probably damaging	Het
Strap	T	G	6: 137,735,382 (GRCm38)	C10G	probably damaging	Het
Tcf7	C	A	11: 52,282,966 (GRCm38)		probably benign	Het
Tmem114	A	T	16: 8,412,190 (GRCm38)	M116K	probably damaging	Het
Tmem63b	A	G	17: 45,677,958 (GRCm38)	S113P	probably damaging	Het
Tmtc3	A	C	10: 100,449,026 (GRCm38)	N582K	probably damaging	Het
Tnfrsf11b	G	A	15: 54,256,182 (GRCm38)		probably benign	Het
Tns2	T	C	15: 102,113,837 (GRCm38)		probably null	Het
Trim30a	A	G	7: 104,411,141 (GRCm38)	V476A	probably damaging	Het
Ush2a	T	A	1: 188,399,954 (GRCm38)	V791D	probably benign	Het
Wdr35	A	T	12: 8,986,077 (GRCm38)	I283F	probably benign	Het
Wnt10b	G	T	15: 98,774,338 (GRCm38)	A166E	possibly damaging	Het

Other mutations in Vmn2r99

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00503:Vmn2r99	APN	17	19,378,854 (GRCm38)	missense	probably benign	0.01
IGL01113:Vmn2r99	APN	17	19,394,256 (GRCm38)	missense	probably benign	0.20
IGL01138:Vmn2r99	APN	17	19,382,623 (GRCm38)	missense	probably damaging	0.97
IGL01646:Vmn2r99	APN	17	19,393,658 (GRCm38)	splice site	probably benign
IGL01769:Vmn2r99	APN	17	19,380,115 (GRCm38)	missense	probably damaging	1.00
IGL02112:Vmn2r99	APN	17	19,380,232 (GRCm38)	missense	probably null	0.99
IGL02891:Vmn2r99	APN	17	19,378,690 (GRCm38)	nonsense	probably null
IGL03132:Vmn2r99	APN	17	19,378,223 (GRCm38)	nonsense	probably null
FR4548:Vmn2r99	UTSW	17	19,394,285 (GRCm38)	missense	probably damaging	0.97
FR4976:Vmn2r99	UTSW	17	19,394,285 (GRCm38)	missense	probably damaging	0.97
PIT4382001:Vmn2r99	UTSW	17	19,394,343 (GRCm38)	missense	probably damaging	1.00
R0196:Vmn2r99	UTSW	17	19,394,573 (GRCm38)	missense	probably benign	0.00
R0720:Vmn2r99	UTSW	17	19,379,043 (GRCm38)	missense	probably benign	0.00
R1501:Vmn2r99	UTSW	17	19,362,259 (GRCm38)	missense	possibly damaging	0.93
R1519:Vmn2r99	UTSW	17	19,380,060 (GRCm38)	missense	probably benign	0.00
R1670:Vmn2r99	UTSW	17	19,362,252 (GRCm38)	missense	probably benign	0.37
R1682:Vmn2r99	UTSW	17	19,377,945 (GRCm38)	missense	probably damaging	0.97
R1873:Vmn2r99	UTSW	17	19,362,153 (GRCm38)	missense	probably benign	0.25
R1967:Vmn2r99	UTSW	17	19,378,815 (GRCm38)	missense	probably benign	0.01
R2101:Vmn2r99	UTSW	17	19,377,991 (GRCm38)	missense	probably damaging	1.00
R2474:Vmn2r99	UTSW	17	19,378,629 (GRCm38)	missense	probably benign	0.04
R2519:Vmn2r99	UTSW	17	19,378,708 (GRCm38)	missense	probably damaging	0.99
R3947:Vmn2r99	UTSW	17	19,378,990 (GRCm38)	missense	probably benign	0.40
R3949:Vmn2r99	UTSW	17	19,378,990 (GRCm38)	missense	probably benign	0.40
R4016:Vmn2r99	UTSW	17	19,378,570 (GRCm38)	missense	possibly damaging	0.86
R4413:Vmn2r99	UTSW	17	19,379,260 (GRCm38)	missense	probably damaging	1.00
R4594:Vmn2r99	UTSW	17	19,393,662 (GRCm38)	missense	probably damaging	1.00
R4999:Vmn2r99	UTSW	17	19,362,135 (GRCm38)	start codon destroyed	probably null	0.96
R5206:Vmn2r99	UTSW	17	19,378,606 (GRCm38)	missense	probably benign	0.40
R5362:Vmn2r99	UTSW	17	19,379,339 (GRCm38)	missense	probably benign	0.00
R5377:Vmn2r99	UTSW	17	19,379,269 (GRCm38)	missense	probably damaging	1.00
R5455:Vmn2r99	UTSW	17	19,394,146 (GRCm38)	nonsense	probably null
R6021:Vmn2r99	UTSW	17	19,377,948 (GRCm38)	missense	probably damaging	1.00
R6059:Vmn2r99	UTSW	17	19,378,980 (GRCm38)	missense	probably benign	0.00
R6214:Vmn2r99	UTSW	17	19,382,558 (GRCm38)	missense	probably benign	0.19
R6215:Vmn2r99	UTSW	17	19,382,558 (GRCm38)	missense	probably benign	0.19
R6313:Vmn2r99	UTSW	17	19,382,605 (GRCm38)	missense	probably damaging	1.00
R6646:Vmn2r99	UTSW	17	19,380,031 (GRCm38)	missense	probably damaging	1.00
R6810:Vmn2r99	UTSW	17	19,380,034 (GRCm38)	missense	probably benign	0.20
R6885:Vmn2r99	UTSW	17	19,380,195 (GRCm38)	missense	possibly damaging	0.52
R6991:Vmn2r99	UTSW	17	19,378,110 (GRCm38)	missense	probably benign	0.03
R7060:Vmn2r99	UTSW	17	19,394,564 (GRCm38)	nonsense	probably null
R7090:Vmn2r99	UTSW	17	19,393,710 (GRCm38)	missense	possibly damaging	0.83
R7094:Vmn2r99	UTSW	17	19,379,311 (GRCm38)	missense	probably benign	0.00
R7449:Vmn2r99	UTSW	17	19,379,145 (GRCm38)	missense	probably benign	0.01
R7789:Vmn2r99	UTSW	17	19,393,817 (GRCm38)	missense	possibly damaging	0.91
R8039:Vmn2r99	UTSW	17	19,380,040 (GRCm38)	missense	probably benign	0.00
R8493:Vmn2r99	UTSW	17	19,393,758 (GRCm38)	missense	probably benign	0.15
R8511:Vmn2r99	UTSW	17	19,394,181 (GRCm38)	missense	probably damaging	1.00
R8715:Vmn2r99	UTSW	17	19,393,660 (GRCm38)	critical splice acceptor site	probably benign
R9462:Vmn2r99	UTSW	17	19,378,126 (GRCm38)	nonsense	probably null
R9681:Vmn2r99	UTSW	17	19,378,627 (GRCm38)	missense	probably damaging	1.00
R9737:Vmn2r99	UTSW	17	19,362,301 (GRCm38)	missense	probably benign
Z1088:Vmn2r99	UTSW	17	19,379,301 (GRCm38)	missense	probably benign	0.18

Predicted Primers

PCR Primer
(F):5'- TGGGCATATCATCATTTTGTGC -3'
(R):5'- GGAATTGAGCCATCAGGTCTAAG -3'

Sequencing Primer
(F):5'- CATTTTGTGCAACAAGGGCTCAG -3'
(R):5'- TTGAGCCATCAGGTCTAAGACTCAG -3'

Posted On

2015-04-17