Incidental Mutation 'R3886:Fn1'
ID 309626
Institutional Source Beutler Lab
Gene Symbol Fn1
Ensembl Gene ENSMUSG00000026193
Gene Name fibronectin 1
Synonyms Fn-1, Fn
MMRRC Submission 040798-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R3886 (G1)
Quality Score 225
Status Not validated
Chromosome 1
Chromosomal Location 71585520-71653200 bp(-) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) T to C at 71640306 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Tyrosine to Cysteine at position 511 (Y511C)
Ref Sequence ENSEMBL: ENSMUSP00000140816 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000055226] [ENSMUST00000186129] [ENSMUST00000186940] [ENSMUST00000187938] [ENSMUST00000188674] [ENSMUST00000188894] [ENSMUST00000189821] [ENSMUST00000190780]
AlphaFold P11276
Predicted Effect probably damaging
Transcript: ENSMUST00000055226
AA Change: Y511C

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000054499
Gene: ENSMUSG00000026193
AA Change: Y511C

DomainStartEndE-ValueType
low complexity region 2 19 N/A INTRINSIC
FN1 53 93 6.38e-18 SMART
FN1 98 141 6.04e-19 SMART
FN1 142 185 6.94e-19 SMART
FN1 187 231 3.47e-19 SMART
FN1 232 276 6.04e-19 SMART
FN1 308 347 5.91e-13 SMART
FN2 353 401 6.66e-30 SMART
FN2 413 461 5.05e-30 SMART
FN1 470 513 2.59e-18 SMART
FN1 518 560 1.68e-17 SMART
FN1 561 604 5.69e-15 SMART
FN3 609 688 3.79e-2 SMART
FN3 719 798 1.27e-3 SMART
FN3 810 888 7.92e-14 SMART
FN3 906 985 4.28e-10 SMART
FN3 996 1074 2.53e-12 SMART
FN3 1086 1160 1.31e-5 SMART
FN3 1173 1255 1.13e-9 SMART
FN3 1264 1346 4.22e-9 SMART
FN3 1357 1437 9.6e-13 SMART
FN3 1448 1527 1.82e-13 SMART
FN3 1538 1617 6.69e-12 SMART
FN3 1632 1711 2.72e-12 SMART
FN3 1722 1801 8.9e-8 SMART
FN3 1812 1891 1.66e-7 SMART
FN3 1904 1983 4.92e-10 SMART
FN3 1993 2074 3.64e-13 SMART
low complexity region 2148 2165 N/A INTRINSIC
FN3 2193 2272 2.9e0 SMART
FN1 2296 2340 3.72e-19 SMART
FN1 2341 2383 2.49e-20 SMART
FN1 2385 2425 2.69e-16 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000186129
AA Change: Y511C

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000141123
Gene: ENSMUSG00000026193
AA Change: Y511C

DomainStartEndE-ValueType
low complexity region 2 19 N/A INTRINSIC
FN1 53 93 6.38e-18 SMART
FN1 98 141 6.04e-19 SMART
FN1 142 185 6.94e-19 SMART
FN1 187 231 3.47e-19 SMART
FN1 232 276 6.04e-19 SMART
FN1 308 347 5.91e-13 SMART
FN2 353 401 6.66e-30 SMART
FN2 413 461 5.05e-30 SMART
FN1 470 513 2.59e-18 SMART
FN1 518 560 1.68e-17 SMART
FN1 561 604 5.69e-15 SMART
FN3 609 688 3.79e-2 SMART
FN3 719 798 1.27e-3 SMART
FN3 810 888 7.92e-14 SMART
FN3 906 985 4.28e-10 SMART
FN3 996 1074 2.53e-12 SMART
FN3 1086 1160 1.31e-5 SMART
FN3 1173 1255 1.13e-9 SMART
FN3 1266 1346 9.6e-13 SMART
FN3 1357 1436 1.82e-13 SMART
FN3 1447 1526 6.69e-12 SMART
FN3 1541 1620 2.72e-12 SMART
FN3 1631 1710 1.66e-7 SMART
FN3 1723 1802 4.92e-10 SMART
FN3 1812 1893 3.64e-13 SMART
low complexity region 1967 1984 N/A INTRINSIC
FN3 2012 2091 2.9e0 SMART
FN1 2115 2159 3.72e-19 SMART
FN1 2160 2202 2.49e-20 SMART
FN1 2204 2244 2.69e-16 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000186613
Predicted Effect noncoding transcript
Transcript: ENSMUST00000186879
Predicted Effect probably benign
Transcript: ENSMUST00000186940
SMART Domains Protein: ENSMUSP00000140315
Gene: ENSMUSG00000026193

DomainStartEndE-ValueType
signal peptide 1 27 N/A INTRINSIC
FN1 53 93 6.38e-18 SMART
FN1 98 141 6.04e-19 SMART
FN1 142 185 6.94e-19 SMART
FN1 187 231 3.47e-19 SMART
FN1 232 276 6.04e-19 SMART
FN1 308 347 1.77e-11 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000187938
AA Change: Y511C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000140975
Gene: ENSMUSG00000026193
AA Change: Y511C

DomainStartEndE-ValueType
low complexity region 2 19 N/A INTRINSIC
FN1 53 93 6.38e-18 SMART
FN1 98 141 6.04e-19 SMART
FN1 142 185 6.94e-19 SMART
FN1 187 231 3.47e-19 SMART
FN1 232 276 6.04e-19 SMART
FN1 308 347 5.91e-13 SMART
FN2 353 401 6.66e-30 SMART
FN2 413 461 5.05e-30 SMART
FN1 470 513 2.59e-18 SMART
FN1 518 560 1.68e-17 SMART
FN1 561 604 5.69e-15 SMART
FN3 609 688 3.79e-2 SMART
FN3 719 798 1.27e-3 SMART
FN3 810 888 7.92e-14 SMART
FN3 906 985 4.28e-10 SMART
FN3 996 1074 2.53e-12 SMART
FN3 1086 1160 1.31e-5 SMART
FN3 1173 1255 1.13e-9 SMART
FN3 1266 1346 9.6e-13 SMART
FN3 1357 1436 1.82e-13 SMART
FN3 1447 1526 6.69e-12 SMART
FN3 1541 1620 2.72e-12 SMART
FN3 1631 1710 8.9e-8 SMART
FN3 1721 1800 1.66e-7 SMART
FN3 1813 1892 4.92e-10 SMART
FN3 1902 1983 3.64e-13 SMART
low complexity region 2032 2049 N/A INTRINSIC
FN3 2077 2156 2.9e0 SMART
FN1 2180 2224 3.72e-19 SMART
FN1 2225 2267 2.49e-20 SMART
FN1 2269 2309 2.69e-16 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000188674
AA Change: Y511C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000140907
Gene: ENSMUSG00000026193
AA Change: Y511C

DomainStartEndE-ValueType
low complexity region 2 19 N/A INTRINSIC
FN1 53 93 6.38e-18 SMART
FN1 98 141 6.04e-19 SMART
FN1 142 185 6.94e-19 SMART
FN1 187 231 3.47e-19 SMART
FN1 232 276 6.04e-19 SMART
FN1 308 347 5.91e-13 SMART
FN2 353 401 6.66e-30 SMART
FN2 413 461 5.05e-30 SMART
FN1 470 513 2.59e-18 SMART
FN1 518 560 1.68e-17 SMART
FN1 561 604 5.69e-15 SMART
FN3 609 688 3.79e-2 SMART
FN3 719 798 1.27e-3 SMART
FN3 810 888 7.92e-14 SMART
FN3 906 985 4.28e-10 SMART
FN3 996 1074 2.53e-12 SMART
FN3 1086 1160 1.31e-5 SMART
FN3 1173 1255 1.13e-9 SMART
FN3 1266 1346 9.6e-13 SMART
FN3 1357 1436 1.82e-13 SMART
FN3 1447 1526 6.69e-12 SMART
FN3 1541 1620 2.72e-12 SMART
FN3 1631 1710 8.9e-8 SMART
FN3 1721 1800 1.66e-7 SMART
FN3 1813 1892 4.92e-10 SMART
FN3 1902 1981 6.79e-13 SMART
FN3 1983 2061 1.01e1 SMART
FN1 2085 2129 3.72e-19 SMART
FN1 2130 2172 2.49e-20 SMART
FN1 2174 2214 2.69e-16 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000188894
AA Change: Y511C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000140471
Gene: ENSMUSG00000026193
AA Change: Y511C

DomainStartEndE-ValueType
low complexity region 2 19 N/A INTRINSIC
FN1 53 93 6.38e-18 SMART
FN1 98 141 6.04e-19 SMART
FN1 142 185 6.94e-19 SMART
FN1 187 231 3.47e-19 SMART
FN1 232 276 6.04e-19 SMART
FN1 308 347 5.91e-13 SMART
FN2 353 401 6.66e-30 SMART
FN2 413 461 5.05e-30 SMART
FN1 470 513 2.59e-18 SMART
FN1 518 560 1.68e-17 SMART
FN1 561 604 5.69e-15 SMART
FN3 609 688 3.79e-2 SMART
FN3 719 798 1.27e-3 SMART
FN3 810 888 7.92e-14 SMART
FN3 906 985 4.28e-10 SMART
FN3 996 1074 2.53e-12 SMART
FN3 1086 1160 1.31e-5 SMART
FN3 1173 1255 1.13e-9 SMART
FN3 1266 1346 9.6e-13 SMART
FN3 1357 1436 1.82e-13 SMART
FN3 1447 1526 6.69e-12 SMART
FN3 1541 1620 2.72e-12 SMART
FN3 1631 1710 8.9e-8 SMART
FN3 1721 1800 1.66e-7 SMART
FN3 1813 1892 4.92e-10 SMART
FN3 1902 1983 3.64e-13 SMART
low complexity region 2057 2074 N/A INTRINSIC
FN3 2102 2181 2.9e0 SMART
FN1 2205 2249 3.72e-19 SMART
FN1 2250 2292 2.49e-20 SMART
FN1 2294 2334 2.69e-16 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000189821
AA Change: Y511C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000139702
Gene: ENSMUSG00000026193
AA Change: Y511C

DomainStartEndE-ValueType
low complexity region 2 19 N/A INTRINSIC
FN1 53 93 6.38e-18 SMART
FN1 98 141 6.04e-19 SMART
FN1 142 185 6.94e-19 SMART
FN1 187 231 3.47e-19 SMART
FN1 232 276 6.04e-19 SMART
FN1 308 347 5.91e-13 SMART
FN2 353 401 6.66e-30 SMART
FN2 413 461 5.05e-30 SMART
FN1 470 513 2.59e-18 SMART
FN1 518 560 1.68e-17 SMART
FN1 561 604 5.69e-15 SMART
FN3 609 688 3.79e-2 SMART
FN3 719 798 1.27e-3 SMART
FN3 810 888 7.92e-14 SMART
FN3 906 985 4.28e-10 SMART
FN3 996 1074 2.53e-12 SMART
FN3 1086 1160 1.31e-5 SMART
FN3 1173 1255 1.13e-9 SMART
FN3 1266 1346 9.6e-13 SMART
FN3 1357 1436 1.82e-13 SMART
FN3 1447 1526 6.69e-12 SMART
FN3 1541 1620 2.72e-12 SMART
FN3 1631 1710 1.66e-7 SMART
FN3 1723 1802 4.92e-10 SMART
FN3 1812 1891 6.79e-13 SMART
FN3 1893 1971 1.01e1 SMART
FN1 1995 2039 3.72e-19 SMART
FN1 2040 2082 2.49e-20 SMART
FN1 2084 2124 2.69e-16 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000190780
AA Change: Y511C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000140816
Gene: ENSMUSG00000026193
AA Change: Y511C

DomainStartEndE-ValueType
low complexity region 2 19 N/A INTRINSIC
FN1 53 93 6.38e-18 SMART
FN1 98 141 6.04e-19 SMART
FN1 142 185 6.94e-19 SMART
FN1 187 231 3.47e-19 SMART
FN1 232 276 6.04e-19 SMART
FN1 308 347 5.91e-13 SMART
FN2 353 401 6.66e-30 SMART
FN2 413 461 5.05e-30 SMART
FN1 470 513 2.59e-18 SMART
FN1 518 560 1.68e-17 SMART
FN1 561 604 5.69e-15 SMART
FN3 609 688 3.79e-2 SMART
FN3 719 798 1.27e-3 SMART
FN3 810 888 7.92e-14 SMART
FN3 906 985 4.28e-10 SMART
FN3 996 1074 2.53e-12 SMART
FN3 1086 1160 1.31e-5 SMART
FN3 1173 1255 1.13e-9 SMART
FN3 1266 1346 9.6e-13 SMART
FN3 1357 1436 1.82e-13 SMART
FN3 1447 1526 6.69e-12 SMART
FN3 1541 1620 2.72e-12 SMART
FN3 1631 1710 1.66e-7 SMART
FN3 1723 1802 4.92e-10 SMART
FN3 1812 1893 3.64e-13 SMART
low complexity region 1942 1959 N/A INTRINSIC
FN3 1987 2066 2.9e0 SMART
FN1 2090 2134 3.72e-19 SMART
FN1 2135 2177 2.49e-20 SMART
FN1 2179 2219 2.69e-16 SMART
Meta Mutation Damage Score 0.7327 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.7%
  • 10x: 97.4%
  • 20x: 95.6%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]
PHENOTYPE: Mutants are defective in mesodermal function. Null mutants are embryonic lethal with major patterning and organizational defects. Conditional mutants live and show increased neuronal apoptosis and susceptibility to induced cerebral ischemia. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 42 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4933407L21Rik T A 1: 85,940,551 (GRCm38) probably null Het
Adam17 G A 12: 21,325,587 (GRCm38) R744C probably damaging Het
Adss2 A G 1: 177,767,769 (GRCm38) Y402H probably damaging Het
Ccdc73 A T 2: 104,991,343 (GRCm38) T546S possibly damaging Het
Cd22 T C 7: 30,870,107 (GRCm38) D354G possibly damaging Het
Chchd6 T C 6: 89,467,451 (GRCm38) E183G probably damaging Het
Col6a5 A G 9: 105,930,930 (GRCm38) L973P unknown Het
Cp T C 3: 19,989,111 (GRCm38) L1021P probably damaging Het
Cplane1 T A 15: 8,171,805 (GRCm38) V22E probably damaging Het
Cps1 C A 1: 67,165,500 (GRCm38) T493K possibly damaging Het
D630045J12Rik A G 6: 38,142,698 (GRCm38) V1703A possibly damaging Het
Dennd1a T C 2: 37,858,077 (GRCm38) N376S possibly damaging Het
Dmxl1 T A 18: 49,878,259 (GRCm38) M1161K probably damaging Het
Ect2l C T 10: 18,168,458 (GRCm38) V310M probably damaging Het
Foxd2 T C 4: 114,908,286 (GRCm38) H179R unknown Het
Gm8674 T C 13: 49,902,163 (GRCm38) noncoding transcript Het
Ice1 T C 13: 70,605,370 (GRCm38) T866A probably benign Het
Jade2 C T 11: 51,830,499 (GRCm38) V201I possibly damaging Het
Kcnb2 T C 1: 15,710,415 (GRCm38) S504P probably damaging Het
Kcng4 T C 8: 119,633,247 (GRCm38) K130R probably benign Het
Lcor T A 19: 41,558,356 (GRCm38) S126R probably damaging Het
Lct T C 1: 128,304,226 (GRCm38) M629V probably damaging Het
Lrch2 C G X: 147,473,007 (GRCm38) A437P probably damaging Het
Lrrk1 C T 7: 66,292,364 (GRCm38) V709I probably damaging Het
Mdh1 A G 11: 21,559,832 (GRCm38) V181A probably damaging Het
Or10g7 A G 9: 39,994,539 (GRCm38) H243R probably damaging Het
Or4a71 T A 2: 89,527,732 (GRCm38) H226L possibly damaging Het
Ppp1r3a A C 6: 14,719,912 (GRCm38) D334E possibly damaging Het
Pramel17 T C 4: 101,835,723 (GRCm38) K360R probably benign Het
Ptpro T A 6: 137,443,594 (GRCm38) V1007D probably damaging Het
Rbm45 A G 2: 76,375,424 (GRCm38) S207G probably benign Het
Relch A G 1: 105,692,213 (GRCm38) N331S probably benign Het
Robo3 G A 9: 37,422,181 (GRCm38) Q723* probably null Het
Rreb1 G A 13: 37,898,506 (GRCm38) probably null Het
Slc35f2 T A 9: 53,816,957 (GRCm38) S372T probably benign Het
Slitrk5 T A 14: 111,679,797 (GRCm38) C284* probably null Het
Snapc4 G A 2: 26,365,498 (GRCm38) Q1005* probably null Het
Tent4b C A 8: 88,200,415 (GRCm38) A151E probably benign Het
Tnxb A G 17: 34,718,911 (GRCm38) D3896G probably damaging Het
Tti1 A G 2: 158,008,950 (GRCm38) V123A possibly damaging Het
Usp1 T C 4: 98,929,736 (GRCm38) C147R probably damaging Het
Vill A G 9: 119,066,714 (GRCm38) N106S probably benign Het
Other mutations in Fn1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00236:Fn1 APN 1 71,652,873 (GRCm38) missense probably benign 0.28
IGL00402:Fn1 APN 1 71,641,163 (GRCm38) missense probably damaging 1.00
IGL00946:Fn1 APN 1 71,645,540 (GRCm38) splice site probably benign
IGL01311:Fn1 APN 1 71,628,140 (GRCm38) missense probably damaging 1.00
IGL01338:Fn1 APN 1 71,626,210 (GRCm38) missense probably damaging 0.98
IGL01353:Fn1 APN 1 71,586,939 (GRCm38) missense probably damaging 1.00
IGL01674:Fn1 APN 1 71,606,741 (GRCm38) missense probably damaging 1.00
IGL01701:Fn1 APN 1 71,629,853 (GRCm38) splice site probably benign
IGL01734:Fn1 APN 1 71,619,485 (GRCm38) missense probably damaging 1.00
IGL01788:Fn1 APN 1 71,613,837 (GRCm38) missense probably damaging 1.00
IGL02186:Fn1 APN 1 71,638,534 (GRCm38) missense probably damaging 1.00
IGL02398:Fn1 APN 1 71,618,670 (GRCm38) splice site probably null
IGL02425:Fn1 APN 1 71,641,143 (GRCm38) splice site probably benign
IGL02516:Fn1 APN 1 71,637,323 (GRCm38) missense possibly damaging 0.78
IGL02593:Fn1 APN 1 71,602,432 (GRCm38) missense probably benign
IGL02651:Fn1 APN 1 71,597,676 (GRCm38) missense possibly damaging 0.65
IGL02681:Fn1 APN 1 71,619,482 (GRCm38) missense probably damaging 1.00
IGL02890:Fn1 APN 1 71,598,372 (GRCm38) critical splice donor site probably null
IGL02929:Fn1 APN 1 71,595,662 (GRCm38) critical splice donor site probably null
IGL03036:Fn1 APN 1 71,629,773 (GRCm38) missense probably damaging 1.00
IGL03088:Fn1 APN 1 71,614,038 (GRCm38) splice site probably null
IGL03142:Fn1 APN 1 71,637,296 (GRCm38) missense probably damaging 1.00
IGL03172:Fn1 APN 1 71,641,262 (GRCm38) missense probably damaging 0.99
IGL03184:Fn1 APN 1 71,609,497 (GRCm38) missense probably benign 0.02
IGL03212:Fn1 APN 1 71,641,325 (GRCm38) nonsense probably null
IGL03246:Fn1 APN 1 71,624,296 (GRCm38) missense possibly damaging 0.89
IGL03367:Fn1 APN 1 71,597,553 (GRCm38) missense probably benign 0.27
depth UTSW 1 71,607,913 (GRCm38) missense probably damaging 1.00
flooded UTSW 1 71,597,516 (GRCm38) missense probably benign 0.01
R0684_Fn1_062 UTSW 1 71,595,809 (GRCm38) splice site probably null
series UTSW 1 71,595,786 (GRCm38) missense probably damaging 1.00
PIT4514001:Fn1 UTSW 1 71,628,456 (GRCm38) missense probably benign 0.01
R0008:Fn1 UTSW 1 71,595,720 (GRCm38) missense probably damaging 0.98
R0112:Fn1 UTSW 1 71,609,653 (GRCm38) missense probably damaging 1.00
R0138:Fn1 UTSW 1 71,624,110 (GRCm38) missense possibly damaging 0.82
R0383:Fn1 UTSW 1 71,597,685 (GRCm38) missense probably damaging 0.99
R0386:Fn1 UTSW 1 71,595,786 (GRCm38) missense probably damaging 1.00
R0648:Fn1 UTSW 1 71,597,585 (GRCm38) missense possibly damaging 0.79
R0684:Fn1 UTSW 1 71,595,809 (GRCm38) splice site probably null
R1054:Fn1 UTSW 1 71,586,214 (GRCm38) makesense probably null
R1183:Fn1 UTSW 1 71,586,245 (GRCm38) missense probably damaging 0.98
R1405:Fn1 UTSW 1 71,642,078 (GRCm38) missense probably damaging 1.00
R1405:Fn1 UTSW 1 71,642,078 (GRCm38) missense probably damaging 1.00
R1414:Fn1 UTSW 1 71,601,303 (GRCm38) splice site probably benign
R1677:Fn1 UTSW 1 71,597,655 (GRCm38) missense probably benign 0.00
R1773:Fn1 UTSW 1 71,637,383 (GRCm38) missense probably damaging 1.00
R1830:Fn1 UTSW 1 71,624,259 (GRCm38) missense probably damaging 1.00
R1987:Fn1 UTSW 1 71,651,625 (GRCm38) missense probably damaging 1.00
R1989:Fn1 UTSW 1 71,651,625 (GRCm38) missense probably damaging 1.00
R2068:Fn1 UTSW 1 71,600,439 (GRCm38) missense probably damaging 1.00
R2113:Fn1 UTSW 1 71,626,164 (GRCm38) missense probably damaging 1.00
R2145:Fn1 UTSW 1 71,606,004 (GRCm38) missense probably damaging 1.00
R2246:Fn1 UTSW 1 71,628,535 (GRCm38) missense probably benign 0.10
R2273:Fn1 UTSW 1 71,613,943 (GRCm38) missense probably null 1.00
R2274:Fn1 UTSW 1 71,613,943 (GRCm38) missense probably null 1.00
R2275:Fn1 UTSW 1 71,613,943 (GRCm38) missense probably null 1.00
R2303:Fn1 UTSW 1 71,614,036 (GRCm38) critical splice acceptor site probably null
R2379:Fn1 UTSW 1 71,649,284 (GRCm38) nonsense probably null
R2382:Fn1 UTSW 1 71,648,119 (GRCm38) missense probably damaging 1.00
R2567:Fn1 UTSW 1 71,597,736 (GRCm38) nonsense probably null
R2864:Fn1 UTSW 1 71,602,419 (GRCm38) missense probably damaging 0.99
R3154:Fn1 UTSW 1 71,593,083 (GRCm38) missense probably damaging 1.00
R3837:Fn1 UTSW 1 71,653,155 (GRCm38) splice site probably null
R3844:Fn1 UTSW 1 71,609,574 (GRCm38) missense possibly damaging 0.61
R3887:Fn1 UTSW 1 71,640,306 (GRCm38) missense probably damaging 1.00
R3888:Fn1 UTSW 1 71,640,306 (GRCm38) missense probably damaging 1.00
R3889:Fn1 UTSW 1 71,640,306 (GRCm38) missense probably damaging 1.00
R3905:Fn1 UTSW 1 71,607,913 (GRCm38) missense probably damaging 1.00
R3906:Fn1 UTSW 1 71,607,913 (GRCm38) missense probably damaging 1.00
R3907:Fn1 UTSW 1 71,607,913 (GRCm38) missense probably damaging 1.00
R3909:Fn1 UTSW 1 71,607,913 (GRCm38) missense probably damaging 1.00
R4611:Fn1 UTSW 1 71,624,178 (GRCm38) nonsense probably null
R4724:Fn1 UTSW 1 71,648,148 (GRCm38) critical splice acceptor site probably null
R4732:Fn1 UTSW 1 71,602,512 (GRCm38) splice site probably null
R4733:Fn1 UTSW 1 71,602,512 (GRCm38) splice site probably null
R4756:Fn1 UTSW 1 71,590,808 (GRCm38) missense probably damaging 1.00
R4809:Fn1 UTSW 1 71,652,800 (GRCm38) intron probably benign
R4839:Fn1 UTSW 1 71,642,083 (GRCm38) missense probably damaging 1.00
R4915:Fn1 UTSW 1 71,595,809 (GRCm38) splice site probably null
R4917:Fn1 UTSW 1 71,595,809 (GRCm38) splice site probably null
R4918:Fn1 UTSW 1 71,595,809 (GRCm38) splice site probably null
R5002:Fn1 UTSW 1 71,629,728 (GRCm38) missense possibly damaging 0.48
R5015:Fn1 UTSW 1 71,626,177 (GRCm38) missense probably damaging 0.98
R5022:Fn1 UTSW 1 71,624,179 (GRCm38) missense probably damaging 1.00
R5109:Fn1 UTSW 1 71,649,235 (GRCm38) missense probably damaging 1.00
R5267:Fn1 UTSW 1 71,629,704 (GRCm38) missense probably damaging 1.00
R5323:Fn1 UTSW 1 71,597,432 (GRCm38) missense probably benign 0.09
R5333:Fn1 UTSW 1 71,624,180 (GRCm38) missense probably damaging 1.00
R5631:Fn1 UTSW 1 71,590,196 (GRCm38) missense probably damaging 1.00
R5644:Fn1 UTSW 1 71,627,250 (GRCm38) missense probably damaging 1.00
R5754:Fn1 UTSW 1 71,600,322 (GRCm38) missense probably damaging 1.00
R5807:Fn1 UTSW 1 71,648,059 (GRCm38) missense probably damaging 1.00
R6053:Fn1 UTSW 1 71,599,290 (GRCm38) missense probably damaging 1.00
R6133:Fn1 UTSW 1 71,597,727 (GRCm38) missense probably damaging 1.00
R6186:Fn1 UTSW 1 71,637,290 (GRCm38) missense probably damaging 1.00
R6270:Fn1 UTSW 1 71,637,275 (GRCm38) missense probably damaging 1.00
R6332:Fn1 UTSW 1 71,628,071 (GRCm38) missense probably benign 0.01
R6431:Fn1 UTSW 1 71,647,844 (GRCm38) splice site probably null
R6571:Fn1 UTSW 1 71,626,190 (GRCm38) missense probably damaging 1.00
R6596:Fn1 UTSW 1 71,609,482 (GRCm38) missense probably damaging 1.00
R6862:Fn1 UTSW 1 71,613,907 (GRCm38) missense probably benign 0.43
R6898:Fn1 UTSW 1 71,600,413 (GRCm38) missense probably damaging 1.00
R6984:Fn1 UTSW 1 71,626,079 (GRCm38) missense probably damaging 1.00
R7107:Fn1 UTSW 1 71,627,249 (GRCm38) missense probably damaging 1.00
R7121:Fn1 UTSW 1 71,600,538 (GRCm38) intron probably benign
R7127:Fn1 UTSW 1 71,597,544 (GRCm38) missense probably benign 0.16
R7194:Fn1 UTSW 1 71,602,323 (GRCm38) missense probably damaging 1.00
R7274:Fn1 UTSW 1 71,628,113 (GRCm38) missense probably benign
R7285:Fn1 UTSW 1 71,637,339 (GRCm38) missense probably damaging 1.00
R7426:Fn1 UTSW 1 71,649,225 (GRCm38) missense probably damaging 1.00
R7453:Fn1 UTSW 1 71,590,880 (GRCm38) missense probably damaging 1.00
R7508:Fn1 UTSW 1 71,597,516 (GRCm38) missense probably benign 0.01
R7724:Fn1 UTSW 1 71,603,735 (GRCm38) missense probably benign 0.02
R7848:Fn1 UTSW 1 71,650,601 (GRCm38) missense probably damaging 1.00
R7992:Fn1 UTSW 1 71,599,666 (GRCm38) missense probably benign 0.34
R8036:Fn1 UTSW 1 71,590,151 (GRCm38) nonsense probably null
R8077:Fn1 UTSW 1 71,612,602 (GRCm38) missense probably damaging 1.00
R8175:Fn1 UTSW 1 71,599,665 (GRCm38) missense probably damaging 1.00
R8177:Fn1 UTSW 1 71,609,587 (GRCm38) missense probably benign
R8212:Fn1 UTSW 1 71,642,905 (GRCm38) missense probably benign 0.01
R8322:Fn1 UTSW 1 71,628,459 (GRCm38) missense probably benign 0.04
R8745:Fn1 UTSW 1 71,637,369 (GRCm38) missense probably benign 0.00
R8780:Fn1 UTSW 1 71,643,149 (GRCm38) missense probably benign 0.00
R8805:Fn1 UTSW 1 71,605,080 (GRCm38) missense probably benign 0.27
R8927:Fn1 UTSW 1 71,599,376 (GRCm38) missense probably benign 0.16
R8928:Fn1 UTSW 1 71,599,376 (GRCm38) missense probably benign 0.16
R8928:Fn1 UTSW 1 71,602,618 (GRCm38) intron probably benign
R8989:Fn1 UTSW 1 71,624,287 (GRCm38) missense possibly damaging 0.95
R8991:Fn1 UTSW 1 71,637,332 (GRCm38) missense probably benign 0.05
R9095:Fn1 UTSW 1 71,607,990 (GRCm38) missense probably null 0.02
R9455:Fn1 UTSW 1 71,607,953 (GRCm38) missense probably benign
R9589:Fn1 UTSW 1 71,629,782 (GRCm38) missense probably damaging 1.00
R9631:Fn1 UTSW 1 71,586,228 (GRCm38) missense probably benign 0.01
R9645:Fn1 UTSW 1 71,628,470 (GRCm38) missense probably benign 0.35
R9723:Fn1 UTSW 1 71,624,210 (GRCm38) missense possibly damaging 0.95
X0023:Fn1 UTSW 1 71,598,373 (GRCm38) critical splice donor site probably null
Z1088:Fn1 UTSW 1 71,649,292 (GRCm38) missense probably damaging 1.00
Z1176:Fn1 UTSW 1 71,597,411 (GRCm38) missense probably benign 0.10
Predicted Primers PCR Primer
(F):5'- GATCTCTCTTTGGGAAAGCCATC -3'
(R):5'- GTTCAAAACTCCACCTTCATCCA -3'

Sequencing Primer
(F):5'- GGCTCAACCTAAATGCTTAC -3'
(R):5'- CAGCTACATGCATGCAAGTC -3'
Posted On 2015-04-17