Incidental Mutation 'R3887:Ncapg'
ID 309688
Institutional Source Beutler Lab
Gene Symbol Ncapg
Ensembl Gene ENSMUSG00000015880
Gene Name non-SMC condensin I complex, subunit G
Synonyms MFT.M05.13, Hcapg, 5730507H05Rik
MMRRC Submission 040799-MU
Accession Numbers
Essential gene? Probably essential (E-score: 0.952) question?
Stock # R3887 (G1)
Quality Score 225
Status Validated
Chromosome 5
Chromosomal Location 45827261-45857888 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to A at 45831705 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Valine to Isoleucine at position 184 (V184I)
Ref Sequence ENSEMBL: ENSMUSP00000112871 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000117396]
AlphaFold E9PWG6
Predicted Effect probably benign
Transcript: ENSMUST00000117396
AA Change: V184I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000112871
Gene: ENSMUSG00000015880
AA Change: V184I

DomainStartEndE-ValueType
Pfam:Cnd3 557 863 7.4e-87 PFAM
low complexity region 864 874 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000129562
Predicted Effect noncoding transcript
Transcript: ENSMUST00000134309
Predicted Effect noncoding transcript
Transcript: ENSMUST00000196242
Predicted Effect noncoding transcript
Transcript: ENSMUST00000198274
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.4%
  • 20x: 95.4%
Validation Efficiency 100% (40/40)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a subunit of the condensin complex, which is responsible for the condensation and stabilization of chromosomes during mitosis and meiosis. Phosphorylation of the encoded protein activates the condensin complex. There are pseudogenes for this gene on chromosomes 8 and 15. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Allele List at MGI
Other mutations in this stock
Total: 37 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4933407L21Rik T A 1: 85,868,273 (GRCm39) probably null Het
Ankdd1a C A 9: 65,409,530 (GRCm39) G469W probably damaging Het
Ano6 A C 15: 95,792,330 (GRCm39) T65P possibly damaging Het
Arhgap26 G A 18: 39,363,019 (GRCm39) probably null Het
Ccdc175 A G 12: 72,182,822 (GRCm39) I399T possibly damaging Het
Ceacam14 T A 7: 17,548,063 (GRCm39) V51D probably damaging Het
Cerk A T 15: 86,033,532 (GRCm39) I297N possibly damaging Het
Cps1 C A 1: 67,204,659 (GRCm39) T493K possibly damaging Het
Dmxl1 T A 18: 50,011,326 (GRCm39) M1161K probably damaging Het
Efcab14 T A 4: 115,595,857 (GRCm39) M1K probably null Het
Etl4 C T 2: 20,534,772 (GRCm39) Q76* probably null Het
Fmc1 T C 6: 38,516,223 (GRCm39) S90P probably benign Het
Fn1 T C 1: 71,679,465 (GRCm39) Y511C probably damaging Het
Foxd2 T C 4: 114,765,483 (GRCm39) H179R unknown Het
Hk2 C T 6: 82,711,942 (GRCm39) D548N possibly damaging Het
Lct T C 1: 128,231,963 (GRCm39) M629V probably damaging Het
Lrp5 G A 19: 3,662,330 (GRCm39) R173C probably damaging Het
Mapk12 A T 15: 89,019,840 (GRCm39) H122Q possibly damaging Het
Mdfic C T 6: 15,799,710 (GRCm39) T279I probably damaging Het
Mycbp2 A G 14: 103,412,233 (GRCm39) V2580A probably damaging Het
Mylk3 A G 8: 86,078,676 (GRCm39) I476T probably damaging Het
Or4a71 T A 2: 89,358,076 (GRCm39) H226L possibly damaging Het
Pla2g15 A G 8: 106,887,767 (GRCm39) Y185C probably damaging Het
Ptpro T A 6: 137,420,592 (GRCm39) V1007D probably damaging Het
Rbm45 A G 2: 76,205,768 (GRCm39) S207G probably benign Het
Reln T C 5: 22,115,847 (GRCm39) I3054V possibly damaging Het
Rreb1 G T 13: 38,077,941 (GRCm39) R51L probably damaging Het
Scube2 A T 7: 109,442,383 (GRCm39) probably benign Het
Slc15a2 A G 16: 36,602,666 (GRCm39) F65S probably damaging Het
Slc17a8 G T 10: 89,427,000 (GRCm39) probably benign Het
Snapc4 G A 2: 26,255,510 (GRCm39) Q1005* probably null Het
Stag3 A G 5: 138,297,101 (GRCm39) I550M probably damaging Het
Steap4 G T 5: 8,030,494 (GRCm39) R450L probably damaging Het
Strn4 T C 7: 16,556,923 (GRCm39) probably benign Het
Stxbp3 C T 3: 108,712,549 (GRCm39) probably null Het
Syngr1 A G 15: 80,000,240 (GRCm39) D117G probably damaging Het
Tbc1d10b A T 7: 126,798,967 (GRCm39) I513N possibly damaging Het
Other mutations in Ncapg
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00662:Ncapg APN 5 45,850,502 (GRCm39) missense possibly damaging 0.53
IGL00777:Ncapg APN 5 45,853,107 (GRCm39) missense possibly damaging 0.93
IGL00857:Ncapg APN 5 45,833,927 (GRCm39) splice site probably null
IGL00916:Ncapg APN 5 45,828,534 (GRCm39) missense probably benign 0.37
IGL01293:Ncapg APN 5 45,839,196 (GRCm39) missense probably benign 0.01
IGL01360:Ncapg APN 5 45,831,727 (GRCm39) nonsense probably null
IGL01462:Ncapg APN 5 45,828,477 (GRCm39) missense probably benign 0.02
IGL01527:Ncapg APN 5 45,829,726 (GRCm39) missense possibly damaging 0.71
IGL01732:Ncapg APN 5 45,851,195 (GRCm39) missense probably damaging 1.00
IGL01788:Ncapg APN 5 45,828,423 (GRCm39) missense probably damaging 0.97
IGL01871:Ncapg APN 5 45,845,923 (GRCm39) missense probably benign 0.09
IGL03106:Ncapg APN 5 45,853,010 (GRCm39) missense probably damaging 1.00
IGL03124:Ncapg APN 5 45,828,551 (GRCm39) missense probably benign
R0086:Ncapg UTSW 5 45,834,086 (GRCm39) splice site probably null
R0109:Ncapg UTSW 5 45,851,090 (GRCm39) splice site probably null
R0110:Ncapg UTSW 5 45,850,489 (GRCm39) unclassified probably benign
R0377:Ncapg UTSW 5 45,851,159 (GRCm39) missense probably benign
R0432:Ncapg UTSW 5 45,829,770 (GRCm39) missense probably damaging 0.99
R0637:Ncapg UTSW 5 45,844,666 (GRCm39) missense probably damaging 1.00
R0835:Ncapg UTSW 5 45,838,790 (GRCm39) missense probably damaging 0.96
R0894:Ncapg UTSW 5 45,837,236 (GRCm39) missense probably null 0.24
R1069:Ncapg UTSW 5 45,833,272 (GRCm39) intron probably benign
R1216:Ncapg UTSW 5 45,857,261 (GRCm39) missense possibly damaging 0.68
R1967:Ncapg UTSW 5 45,857,252 (GRCm39) missense probably damaging 0.99
R2396:Ncapg UTSW 5 45,835,715 (GRCm39) missense probably benign 0.00
R3157:Ncapg UTSW 5 45,833,400 (GRCm39) missense probably benign
R3735:Ncapg UTSW 5 45,853,469 (GRCm39) missense probably benign 0.00
R3736:Ncapg UTSW 5 45,853,469 (GRCm39) missense probably benign 0.00
R4371:Ncapg UTSW 5 45,835,797 (GRCm39) missense probably benign
R4545:Ncapg UTSW 5 45,828,554 (GRCm39) missense probably damaging 1.00
R4546:Ncapg UTSW 5 45,828,554 (GRCm39) missense probably damaging 1.00
R4558:Ncapg UTSW 5 45,833,986 (GRCm39) missense probably benign 0.00
R4615:Ncapg UTSW 5 45,844,741 (GRCm39) missense probably benign 0.00
R4938:Ncapg UTSW 5 45,828,551 (GRCm39) missense probably benign
R5839:Ncapg UTSW 5 45,829,620 (GRCm39) missense probably damaging 0.99
R5871:Ncapg UTSW 5 45,853,039 (GRCm39) missense probably damaging 1.00
R6086:Ncapg UTSW 5 45,850,578 (GRCm39) missense probably damaging 1.00
R6418:Ncapg UTSW 5 45,839,158 (GRCm39) missense probably damaging 1.00
R6617:Ncapg UTSW 5 45,827,474 (GRCm39) missense probably benign 0.03
R7145:Ncapg UTSW 5 45,827,372 (GRCm39) missense possibly damaging 0.82
R7408:Ncapg UTSW 5 45,853,135 (GRCm39) missense probably benign 0.00
R7443:Ncapg UTSW 5 45,829,652 (GRCm39) missense probably benign 0.31
R7463:Ncapg UTSW 5 45,851,434 (GRCm39) splice site probably null
R7509:Ncapg UTSW 5 45,853,450 (GRCm39) missense probably benign 0.01
R7687:Ncapg UTSW 5 45,857,227 (GRCm39) missense probably benign 0.03
R7919:Ncapg UTSW 5 45,853,390 (GRCm39) missense probably benign 0.00
R8022:Ncapg UTSW 5 45,839,136 (GRCm39) missense probably damaging 1.00
R8177:Ncapg UTSW 5 45,851,095 (GRCm39) missense probably benign 0.00
R8261:Ncapg UTSW 5 45,844,730 (GRCm39) missense possibly damaging 0.90
R8263:Ncapg UTSW 5 45,849,134 (GRCm39) missense probably benign 0.44
R8324:Ncapg UTSW 5 45,853,010 (GRCm39) missense probably damaging 1.00
R8333:Ncapg UTSW 5 45,831,805 (GRCm39) missense probably damaging 0.96
R8742:Ncapg UTSW 5 45,851,216 (GRCm39) missense probably damaging 1.00
R9026:Ncapg UTSW 5 45,853,115 (GRCm39) missense probably benign 0.00
R9051:Ncapg UTSW 5 45,853,140 (GRCm39) missense probably damaging 1.00
R9076:Ncapg UTSW 5 45,833,983 (GRCm39) missense probably benign
R9122:Ncapg UTSW 5 45,846,015 (GRCm39) missense possibly damaging 0.95
R9751:Ncapg UTSW 5 45,851,195 (GRCm39) missense probably damaging 1.00
R9776:Ncapg UTSW 5 45,829,834 (GRCm39) missense probably damaging 0.96
RF019:Ncapg UTSW 5 45,856,198 (GRCm39) missense probably benign 0.00
Z1088:Ncapg UTSW 5 45,837,222 (GRCm39) missense probably damaging 1.00
Z1177:Ncapg UTSW 5 45,829,844 (GRCm39) critical splice donor site probably null
Predicted Primers PCR Primer
(F):5'- TCGTCATCTGACCTTTGGGG -3'
(R):5'- CTGAGCAATAGACATAGCTCTCATATG -3'

Sequencing Primer
(F):5'- TTTCAGAAGGAAATGCTAGT -3'
(R):5'- GGTTCATCTTTCAAAAACATGCC -3'
Posted On 2015-04-17