Mutagenetix > Incidental Mutation

Incidental Mutation 'R3750:Zfhx4'

310130

Institutional Source

Beutler Lab

Gene Symbol

Zfhx4

Ensembl Gene

ENSMUSG00000025255

Gene Name

zinc finger homeodomain 4

Synonyms

Zfh-4, Zfh4, C130041O22Rik

MMRRC Submission

040735-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.685) question?

Stock #

R3750 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

5283586-5480917 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 5308225 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Lysine at position 484 (E484K)

Ref Sequence

ENSEMBL: ENSMUSP00000135289 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000026284] [ENSMUST00000175866] [ENSMUST00000176175] [ENSMUST00000176383]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000026284
AA Change: E484K

PolyPhen 2 Score 0.816 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000026284
Gene: ENSMUSG00000025255
AA Change: E484K

Domain	Start	End	E-Value	Type
ZnF_C2H2	80	99	1.78e2	SMART
low complexity region	110	122	N/A	INTRINSIC
ZnF_C2H2	277	300	1.55e1	SMART
low complexity region	421	438	N/A	INTRINSIC
low complexity region	470	475	N/A	INTRINSIC
low complexity region	590	610	N/A	INTRINSIC
ZnF_C2H2	611	634	2.45e0	SMART
ZnF_C2H2	642	665	6.78e-3	SMART
ZnF_U1	694	728	1.8e-1	SMART
ZnF_C2H2	697	721	4.87e-4	SMART
low complexity region	754	763	N/A	INTRINSIC
ZnF_C2H2	765	789	6.67e-2	SMART
ZnF_C2H2	876	897	2.44e2	SMART
ZnF_U1	912	946	2.88e0	SMART
ZnF_C2H2	915	939	1.23e0	SMART
ZnF_C2H2	971	993	7.05e-1	SMART
ZnF_U1	1016	1050	3.73e0	SMART
ZnF_C2H2	1019	1043	4.98e-1	SMART
ZnF_C2H2	1188	1211	1.1e-2	SMART
ZnF_C2H2	1217	1240	4.94e0	SMART
ZnF_C2H2	1368	1390	7.67e-2	SMART
ZnF_C2H2	1396	1419	1.33e-1	SMART
ZnF_U1	1509	1543	7.4e-1	SMART
ZnF_C2H2	1512	1536	8.22e-2	SMART
ZnF_U1	1561	1595	3.73e0	SMART
ZnF_C2H2	1564	1588	1.16e-1	SMART
low complexity region	1664	1692	N/A	INTRINSIC
low complexity region	1701	1713	N/A	INTRINSIC
low complexity region	1762	1808	N/A	INTRINSIC
ZnF_C2H2	1916	1939	3.07e-1	SMART
low complexity region	1964	1990	N/A	INTRINSIC
low complexity region	2008	2032	N/A	INTRINSIC
low complexity region	2055	2072	N/A	INTRINSIC
HOX	2100	2162	4.23e-16	SMART
HOX	2197	2259	5.62e-21	SMART
ZnF_C2H2	2283	2303	1.13e1	SMART
low complexity region	2364	2376	N/A	INTRINSIC
low complexity region	2408	2425	N/A	INTRINSIC
low complexity region	2449	2460	N/A	INTRINSIC
ZnF_C2H2	2461	2483	2.17e-1	SMART
HOX	2573	2635	3.18e-20	SMART
ZnF_C2H2	2643	2666	6.67e-2	SMART
low complexity region	2874	2886	N/A	INTRINSIC
HOX	2896	2958	4.54e-16	SMART
ZnF_U1	2971	3005	6.59e-1	SMART
ZnF_C2H2	2974	2998	1.36e1	SMART
low complexity region	3066	3078	N/A	INTRINSIC
low complexity region	3106	3119	N/A	INTRINSIC
low complexity region	3163	3186	N/A	INTRINSIC
coiled coil region	3279	3308	N/A	INTRINSIC
ZnF_C2H2	3368	3388	1.12e2	SMART
ZnF_U1	3409	3443	6.16e-2	SMART
ZnF_C2H2	3412	3436	6.57e0	SMART
low complexity region	3461	3479	N/A	INTRINSIC
low complexity region	3505	3527	N/A	INTRINSIC
low complexity region	3536	3547	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000175641

Predicted Effect

possibly damaging
Transcript: ENSMUST00000175866
AA Change: E484K

PolyPhen 2 Score 0.816 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000135827
Gene: ENSMUSG00000025255
AA Change: E484K

Domain	Start	End	E-Value	Type
ZnF_C2H2	80	99	1.78e2	SMART
low complexity region	110	122	N/A	INTRINSIC
ZnF_C2H2	277	300	1.55e1	SMART
low complexity region	421	438	N/A	INTRINSIC
low complexity region	470	475	N/A	INTRINSIC
low complexity region	590	610	N/A	INTRINSIC
ZnF_C2H2	611	634	2.45e0	SMART
ZnF_C2H2	642	665	6.78e-3	SMART
ZnF_U1	694	728	1.8e-1	SMART
ZnF_C2H2	697	721	4.87e-4	SMART
low complexity region	754	763	N/A	INTRINSIC
ZnF_C2H2	765	789	6.67e-2	SMART
ZnF_C2H2	902	923	2.44e2	SMART
ZnF_U1	938	972	2.88e0	SMART
ZnF_C2H2	941	965	1.23e0	SMART
ZnF_C2H2	997	1019	7.05e-1	SMART
ZnF_U1	1042	1076	3.73e0	SMART
ZnF_C2H2	1045	1069	4.98e-1	SMART
ZnF_C2H2	1213	1236	1.1e-2	SMART
ZnF_C2H2	1242	1265	4.94e0	SMART
ZnF_C2H2	1393	1415	7.67e-2	SMART
ZnF_C2H2	1421	1444	1.33e-1	SMART
ZnF_U1	1534	1568	7.4e-1	SMART
ZnF_C2H2	1537	1561	8.22e-2	SMART
ZnF_U1	1586	1620	3.73e0	SMART
ZnF_C2H2	1589	1613	1.16e-1	SMART
low complexity region	1689	1717	N/A	INTRINSIC
low complexity region	1726	1738	N/A	INTRINSIC
low complexity region	1787	1833	N/A	INTRINSIC
ZnF_C2H2	1941	1964	3.07e-1	SMART
low complexity region	1989	2015	N/A	INTRINSIC
low complexity region	2033	2057	N/A	INTRINSIC
low complexity region	2080	2097	N/A	INTRINSIC
HOX	2125	2187	4.23e-16	SMART
HOX	2222	2284	5.62e-21	SMART
ZnF_C2H2	2308	2328	1.13e1	SMART
low complexity region	2389	2401	N/A	INTRINSIC
low complexity region	2433	2450	N/A	INTRINSIC
low complexity region	2474	2485	N/A	INTRINSIC
ZnF_C2H2	2486	2508	2.17e-1	SMART
HOX	2598	2660	3.18e-20	SMART
ZnF_C2H2	2668	2691	6.67e-2	SMART
low complexity region	2899	2911	N/A	INTRINSIC
HOX	2921	2983	4.54e-16	SMART
ZnF_U1	2996	3030	6.59e-1	SMART
ZnF_C2H2	2999	3023	1.36e1	SMART
low complexity region	3091	3103	N/A	INTRINSIC
low complexity region	3131	3144	N/A	INTRINSIC
low complexity region	3188	3211	N/A	INTRINSIC
coiled coil region	3304	3333	N/A	INTRINSIC
ZnF_C2H2	3393	3413	1.12e2	SMART
ZnF_U1	3434	3468	6.16e-2	SMART
ZnF_C2H2	3437	3461	6.57e0	SMART
low complexity region	3486	3504	N/A	INTRINSIC
low complexity region	3530	3552	N/A	INTRINSIC
low complexity region	3561	3572	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000176175

SMART Domains

Protein: ENSMUSP00000139253
Gene: ENSMUSG00000025255

Domain	Start	End	E-Value	Type
Blast:ZnF_C2H2	80	99	4e-7	BLAST

Predicted Effect

possibly damaging
Transcript: ENSMUST00000176383
AA Change: E484K

PolyPhen 2 Score 0.816 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000135289
Gene: ENSMUSG00000025255
AA Change: E484K

Domain	Start	End	E-Value	Type
ZnF_C2H2	80	99	1.78e2	SMART
low complexity region	110	122	N/A	INTRINSIC
ZnF_C2H2	277	300	1.55e1	SMART
low complexity region	421	438	N/A	INTRINSIC
low complexity region	470	475	N/A	INTRINSIC
low complexity region	590	610	N/A	INTRINSIC
ZnF_C2H2	611	634	2.45e0	SMART
ZnF_C2H2	642	665	6.78e-3	SMART
ZnF_U1	694	728	1.8e-1	SMART
ZnF_C2H2	697	721	4.87e-4	SMART
low complexity region	754	763	N/A	INTRINSIC
ZnF_C2H2	765	789	6.67e-2	SMART
ZnF_C2H2	876	897	2.44e2	SMART
ZnF_U1	912	946	2.88e0	SMART
ZnF_C2H2	915	939	1.23e0	SMART
ZnF_C2H2	971	993	7.05e-1	SMART
ZnF_U1	1016	1050	3.73e0	SMART
ZnF_C2H2	1019	1043	4.98e-1	SMART
ZnF_C2H2	1188	1211	1.1e-2	SMART
ZnF_C2H2	1217	1240	4.94e0	SMART
ZnF_C2H2	1368	1390	7.67e-2	SMART
ZnF_C2H2	1396	1419	1.33e-1	SMART
ZnF_U1	1509	1543	7.4e-1	SMART
ZnF_C2H2	1512	1536	8.22e-2	SMART
ZnF_U1	1561	1595	3.73e0	SMART
ZnF_C2H2	1564	1588	1.16e-1	SMART
low complexity region	1664	1692	N/A	INTRINSIC
low complexity region	1701	1713	N/A	INTRINSIC
low complexity region	1762	1808	N/A	INTRINSIC
ZnF_C2H2	1916	1939	3.07e-1	SMART
low complexity region	1964	1990	N/A	INTRINSIC
low complexity region	2008	2032	N/A	INTRINSIC
low complexity region	2055	2072	N/A	INTRINSIC
HOX	2100	2162	4.23e-16	SMART
HOX	2197	2259	5.62e-21	SMART
ZnF_C2H2	2283	2303	1.13e1	SMART
low complexity region	2364	2376	N/A	INTRINSIC
low complexity region	2408	2425	N/A	INTRINSIC
low complexity region	2449	2460	N/A	INTRINSIC
ZnF_C2H2	2461	2483	2.17e-1	SMART
HOX	2573	2635	3.18e-20	SMART
ZnF_C2H2	2643	2666	6.67e-2	SMART
low complexity region	2874	2886	N/A	INTRINSIC
HOX	2896	2958	4.54e-16	SMART
ZnF_U1	2971	3005	6.59e-1	SMART
ZnF_C2H2	2974	2998	1.36e1	SMART
low complexity region	3066	3078	N/A	INTRINSIC
low complexity region	3106	3119	N/A	INTRINSIC
low complexity region	3163	3186	N/A	INTRINSIC
coiled coil region	3279	3308	N/A	INTRINSIC
ZnF_C2H2	3368	3388	1.12e2	SMART
ZnF_U1	3409	3443	6.16e-2	SMART
ZnF_C2H2	3412	3436	6.57e0	SMART
low complexity region	3461	3479	N/A	INTRINSIC
low complexity region	3505	3527	N/A	INTRINSIC
low complexity region	3536	3547	N/A	INTRINSIC

Meta Mutation Damage Score

0.0712

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.6%

Validation Efficiency

100% (38/38)

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aasdh	C	A	5: 77,036,501 (GRCm39)	E347*	probably null	Het
Adam12	T	C	7: 133,774,594 (GRCm39)	D5G	probably damaging	Het
Adam26b	A	C	8: 43,974,234 (GRCm39)	V256G	probably benign	Het
Ago1	A	G	4: 126,354,837 (GRCm39)	I125T	probably benign	Het
Bckdk	C	A	7: 127,504,590 (GRCm39)	R105S	probably damaging	Het
Bub1b	T	A	2: 118,445,936 (GRCm39)	N319K	possibly damaging	Het
Clcn6	G	T	4: 148,108,644 (GRCm39)	C128*	probably null	Het
Col6a4	C	A	9: 105,897,864 (GRCm39)		probably null	Het
Cyp4v3	G	A	8: 45,768,745 (GRCm39)	R272*	probably null	Het
Dlg5	G	A	14: 24,215,328 (GRCm39)	A665V	probably damaging	Het
Foxd1	G	C	13: 98,492,424 (GRCm39)	A433P	unknown	Het
Hsd17b3	A	T	13: 64,210,993 (GRCm39)		probably null	Het
Kcnc4	A	G	3: 107,355,506 (GRCm39)	V314A	probably benign	Het
Lrba	T	G	3: 86,283,260 (GRCm39)	L1858R	probably damaging	Het
Marcks	G	A	10: 37,016,866 (GRCm39)		probably benign	Het
Mroh2b	G	A	15: 4,981,728 (GRCm39)	W1513*	probably null	Het
Nefh	A	G	11: 4,889,937 (GRCm39)	V894A	probably benign	Het
Pdzph1	A	T	17: 59,280,331 (GRCm39)	Y650*	probably null	Het
Plce1	A	C	19: 38,766,343 (GRCm39)	I2109L	probably benign	Het
Pramel18	T	A	4: 101,767,073 (GRCm39)	D107E	possibly damaging	Het
Primpol	A	T	8: 47,052,848 (GRCm39)	D154E	probably benign	Het
Relch	C	T	1: 105,681,302 (GRCm39)	T1178I	probably damaging	Het
Rtca	A	T	3: 116,286,650 (GRCm39)	F327L	probably benign	Het
Scn2a	T	G	2: 65,544,115 (GRCm39)	V832G	probably damaging	Het
Secisbp2l	C	T	2: 125,582,657 (GRCm39)	G933D	possibly damaging	Het
Skil	A	G	3: 31,170,983 (GRCm39)	N354S	probably benign	Het
Slk	T	G	19: 47,608,248 (GRCm39)	D400E	possibly damaging	Het
Spata31	G	T	13: 65,069,557 (GRCm39)	L568F	probably benign	Het
Spon1	T	C	7: 113,365,621 (GRCm39)	L19P	probably damaging	Het
Spon1	T	A	7: 113,616,024 (GRCm39)	V297E	possibly damaging	Het
Tas2r136	A	T	6: 132,754,200 (GRCm39)	F309Y	probably damaging	Het
Tcp11l1	A	G	2: 104,528,887 (GRCm39)	I137T	probably damaging	Het
Ttn	G	A	2: 76,584,350 (GRCm39)	H22253Y	probably damaging	Het
Upf1	G	T	8: 70,786,000 (GRCm39)	N975K	possibly damaging	Het
Usp1	C	T	4: 98,822,357 (GRCm39)		probably null	Het
Zswim4	G	A	8: 84,938,676 (GRCm39)	P1069S	possibly damaging	Het

Other mutations in Zfhx4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00332:Zfhx4	APN	3	5,307,401 (GRCm39)	missense	probably damaging	1.00
IGL00915:Zfhx4	APN	3	5,310,583 (GRCm39)	missense	probably damaging	0.99
IGL01145:Zfhx4	APN	3	5,310,407 (GRCm39)	missense	probably damaging	1.00
IGL01302:Zfhx4	APN	3	5,308,628 (GRCm39)	missense	probably damaging	1.00
IGL01314:Zfhx4	APN	3	5,478,154 (GRCm39)	missense	probably damaging	0.98
IGL01321:Zfhx4	APN	3	5,307,388 (GRCm39)	missense	probably benign	0.01
IGL01328:Zfhx4	APN	3	5,309,344 (GRCm39)	missense	probably damaging	1.00
IGL01333:Zfhx4	APN	3	5,464,387 (GRCm39)	missense	probably damaging	1.00
IGL01351:Zfhx4	APN	3	5,466,196 (GRCm39)	missense	probably damaging	1.00
IGL01524:Zfhx4	APN	3	5,309,036 (GRCm39)	missense	probably damaging	1.00
IGL01549:Zfhx4	APN	3	5,464,522 (GRCm39)	missense	probably damaging	1.00
IGL01715:Zfhx4	APN	3	5,307,105 (GRCm39)	missense	probably benign	0.00
IGL01736:Zfhx4	APN	3	5,309,152 (GRCm39)	missense	possibly damaging	0.85
IGL01904:Zfhx4	APN	3	5,477,769 (GRCm39)	missense	probably damaging	1.00
IGL02298:Zfhx4	APN	3	5,309,364 (GRCm39)	splice site	probably null
IGL02342:Zfhx4	APN	3	5,467,434 (GRCm39)	missense	probably benign	0.14
IGL02465:Zfhx4	APN	3	5,464,663 (GRCm39)	missense	possibly damaging	0.48
IGL02481:Zfhx4	APN	3	5,476,903 (GRCm39)	missense	probably damaging	0.99
IGL02511:Zfhx4	APN	3	5,464,243 (GRCm39)	missense	probably damaging	1.00
IGL02571:Zfhx4	APN	3	5,394,583 (GRCm39)	missense	probably damaging	1.00
IGL02685:Zfhx4	APN	3	5,477,213 (GRCm39)	missense	probably damaging	1.00
IGL02721:Zfhx4	APN	3	5,308,367 (GRCm39)	missense	possibly damaging	0.76
IGL02806:Zfhx4	APN	3	5,455,468 (GRCm39)	missense	probably benign	0.00
IGL03140:Zfhx4	APN	3	5,307,585 (GRCm39)	missense	probably damaging	1.00
IGL03185:Zfhx4	APN	3	5,468,974 (GRCm39)	missense	probably benign	0.05
IGL03209:Zfhx4	APN	3	5,466,231 (GRCm39)	missense	probably damaging	1.00
IGL03292:Zfhx4	APN	3	5,476,840 (GRCm39)	nonsense	probably null
IGL03302:Zfhx4	APN	3	5,468,773 (GRCm39)	missense	possibly damaging	0.88
IGL03303:Zfhx4	APN	3	5,468,410 (GRCm39)	missense	probably damaging	1.00
IGL03341:Zfhx4	APN	3	5,476,910 (GRCm39)	missense	probably damaging	0.98
3-1:Zfhx4	UTSW	3	5,468,445 (GRCm39)	missense	probably benign	0.14
B5639:Zfhx4	UTSW	3	5,468,235 (GRCm39)	missense	probably damaging	0.99
IGL02796:Zfhx4	UTSW	3	5,464,599 (GRCm39)	missense	probably damaging	1.00
IGL03047:Zfhx4	UTSW	3	5,308,793 (GRCm39)	missense	probably damaging	0.99
P0025:Zfhx4	UTSW	3	5,464,648 (GRCm39)	missense	probably benign	0.04
PIT4377001:Zfhx4	UTSW	3	5,307,802 (GRCm39)	missense	probably damaging	0.98
R0090:Zfhx4	UTSW	3	5,308,685 (GRCm39)	missense	probably damaging	1.00
R0107:Zfhx4	UTSW	3	5,464,042 (GRCm39)	missense	probably damaging	1.00
R0401:Zfhx4	UTSW	3	5,466,221 (GRCm39)	missense	possibly damaging	0.87
R0465:Zfhx4	UTSW	3	5,310,716 (GRCm39)	splice site	probably benign
R0506:Zfhx4	UTSW	3	5,467,795 (GRCm39)	missense	probably damaging	1.00
R0507:Zfhx4	UTSW	3	5,466,048 (GRCm39)	nonsense	probably null
R0550:Zfhx4	UTSW	3	5,465,554 (GRCm39)	missense	probably damaging	0.99
R0576:Zfhx4	UTSW	3	5,467,161 (GRCm39)	missense	probably damaging	1.00
R0590:Zfhx4	UTSW	3	5,467,693 (GRCm39)	missense	probably damaging	1.00
R0697:Zfhx4	UTSW	3	5,466,793 (GRCm39)	missense	probably damaging	0.99
R0727:Zfhx4	UTSW	3	5,466,133 (GRCm39)	missense	probably damaging	0.98
R0762:Zfhx4	UTSW	3	5,468,880 (GRCm39)	missense	probably damaging	1.00
R0815:Zfhx4	UTSW	3	5,310,375 (GRCm39)	missense	possibly damaging	0.87
R0863:Zfhx4	UTSW	3	5,310,375 (GRCm39)	missense	possibly damaging	0.87
R0866:Zfhx4	UTSW	3	5,477,272 (GRCm39)	missense	possibly damaging	0.58
R1109:Zfhx4	UTSW	3	5,464,930 (GRCm39)	missense	possibly damaging	0.59
R1177:Zfhx4	UTSW	3	5,465,891 (GRCm39)	small deletion	probably benign
R1338:Zfhx4	UTSW	3	5,462,021 (GRCm39)	missense	possibly damaging	0.86
R1388:Zfhx4	UTSW	3	5,466,447 (GRCm39)	missense	probably damaging	1.00
R1434:Zfhx4	UTSW	3	5,306,919 (GRCm39)	missense	probably benign	0.00
R1470:Zfhx4	UTSW	3	5,478,206 (GRCm39)	makesense	probably null
R1470:Zfhx4	UTSW	3	5,478,206 (GRCm39)	makesense	probably null
R1552:Zfhx4	UTSW	3	5,468,170 (GRCm39)	missense	probably damaging	1.00
R1589:Zfhx4	UTSW	3	5,306,789 (GRCm39)	missense	probably damaging	1.00
R1633:Zfhx4	UTSW	3	5,465,473 (GRCm39)	missense	probably damaging	1.00
R1656:Zfhx4	UTSW	3	5,478,076 (GRCm39)	missense	probably damaging	1.00
R1717:Zfhx4	UTSW	3	5,468,164 (GRCm39)	missense	probably benign	0.20
R1739:Zfhx4	UTSW	3	5,466,790 (GRCm39)	missense	probably damaging	1.00
R1760:Zfhx4	UTSW	3	5,447,676 (GRCm39)	missense	probably benign
R1842:Zfhx4	UTSW	3	5,466,558 (GRCm39)	missense	probably damaging	1.00
R1867:Zfhx4	UTSW	3	5,477,774 (GRCm39)	missense	probably damaging	1.00
R1868:Zfhx4	UTSW	3	5,477,774 (GRCm39)	missense	probably damaging	1.00
R2064:Zfhx4	UTSW	3	5,463,987 (GRCm39)	missense	probably damaging	1.00
R2083:Zfhx4	UTSW	3	5,468,223 (GRCm39)	missense	possibly damaging	0.58
R2154:Zfhx4	UTSW	3	5,466,801 (GRCm39)	missense	possibly damaging	0.86
R2165:Zfhx4	UTSW	3	5,468,418 (GRCm39)	missense	probably benign	0.32
R2181:Zfhx4	UTSW	3	5,468,392 (GRCm39)	missense	probably damaging	1.00
R2201:Zfhx4	UTSW	3	5,307,349 (GRCm39)	missense	probably damaging	1.00
R2209:Zfhx4	UTSW	3	5,461,978 (GRCm39)	missense	probably damaging	1.00
R2303:Zfhx4	UTSW	3	5,462,120 (GRCm39)	missense	probably damaging	0.99
R2327:Zfhx4	UTSW	3	5,468,418 (GRCm39)	missense	probably benign	0.32
R2420:Zfhx4	UTSW	3	5,455,465 (GRCm39)	missense	probably benign	0.00
R2422:Zfhx4	UTSW	3	5,455,465 (GRCm39)	missense	probably benign	0.00
R2516:Zfhx4	UTSW	3	5,468,418 (GRCm39)	missense	probably benign	0.32
R2518:Zfhx4	UTSW	3	5,468,418 (GRCm39)	missense	probably benign	0.32
R2519:Zfhx4	UTSW	3	5,468,418 (GRCm39)	missense	probably benign	0.32
R2520:Zfhx4	UTSW	3	5,468,418 (GRCm39)	missense	probably benign	0.32
R2566:Zfhx4	UTSW	3	5,310,203 (GRCm39)	missense	probably damaging	0.98
R2922:Zfhx4	UTSW	3	5,468,724 (GRCm39)	missense	probably damaging	1.00
R3000:Zfhx4	UTSW	3	5,468,714 (GRCm39)	missense	probably damaging	1.00
R3103:Zfhx4	UTSW	3	5,464,386 (GRCm39)	missense	probably damaging	1.00
R3409:Zfhx4	UTSW	3	5,468,418 (GRCm39)	missense	probably benign	0.32
R3414:Zfhx4	UTSW	3	5,468,883 (GRCm39)	missense	probably damaging	1.00
R3746:Zfhx4	UTSW	3	5,308,225 (GRCm39)	missense	possibly damaging	0.82
R3747:Zfhx4	UTSW	3	5,308,225 (GRCm39)	missense	possibly damaging	0.82
R3748:Zfhx4	UTSW	3	5,308,225 (GRCm39)	missense	possibly damaging	0.82
R3749:Zfhx4	UTSW	3	5,308,225 (GRCm39)	missense	possibly damaging	0.82
R3763:Zfhx4	UTSW	3	5,468,404 (GRCm39)	missense	probably damaging	1.00
R3826:Zfhx4	UTSW	3	5,466,269 (GRCm39)	missense	probably damaging	1.00
R3827:Zfhx4	UTSW	3	5,466,269 (GRCm39)	missense	probably damaging	1.00
R3830:Zfhx4	UTSW	3	5,466,269 (GRCm39)	missense	probably damaging	1.00
R3877:Zfhx4	UTSW	3	5,465,845 (GRCm39)	missense	probably benign
R3919:Zfhx4	UTSW	3	5,464,175 (GRCm39)	missense	possibly damaging	0.48
R3922:Zfhx4	UTSW	3	5,465,707 (GRCm39)	missense	probably damaging	1.00
R3927:Zfhx4	UTSW	3	5,468,418 (GRCm39)	missense	probably benign	0.32
R3965:Zfhx4	UTSW	3	5,468,907 (GRCm39)	missense	probably damaging	1.00
R4004:Zfhx4	UTSW	3	5,468,418 (GRCm39)	missense	probably benign	0.32
R4049:Zfhx4	UTSW	3	5,463,919 (GRCm39)	missense	probably damaging	1.00
R4073:Zfhx4	UTSW	3	5,464,384 (GRCm39)	missense	probably damaging	1.00
R4134:Zfhx4	UTSW	3	5,308,687 (GRCm39)	missense	probably damaging	1.00
R4401:Zfhx4	UTSW	3	5,468,405 (GRCm39)	nonsense	probably null
R4439:Zfhx4	UTSW	3	5,279,875 (GRCm39)	unclassified	probably benign
R4497:Zfhx4	UTSW	3	5,464,680 (GRCm39)	missense	possibly damaging	0.88
R4518:Zfhx4	UTSW	3	5,477,578 (GRCm39)	missense	probably damaging	1.00
R4569:Zfhx4	UTSW	3	5,466,894 (GRCm39)	missense	probably benign	0.00
R4612:Zfhx4	UTSW	3	5,462,123 (GRCm39)	missense	probably damaging	1.00
R4616:Zfhx4	UTSW	3	5,478,127 (GRCm39)	missense	possibly damaging	0.66
R4626:Zfhx4	UTSW	3	5,467,699 (GRCm39)	missense	probably damaging	1.00
R4628:Zfhx4	UTSW	3	5,468,536 (GRCm39)	missense	probably damaging	1.00
R4637:Zfhx4	UTSW	3	5,468,464 (GRCm39)	missense	probably damaging	1.00
R4647:Zfhx4	UTSW	3	5,464,341 (GRCm39)	missense	probably damaging	0.99
R4708:Zfhx4	UTSW	3	5,310,563 (GRCm39)	splice site	probably null
R4729:Zfhx4	UTSW	3	5,464,557 (GRCm39)	missense	probably damaging	1.00
R4732:Zfhx4	UTSW	3	5,279,867 (GRCm39)	unclassified	probably benign
R4757:Zfhx4	UTSW	3	5,465,122 (GRCm39)	missense	possibly damaging	0.85
R4765:Zfhx4	UTSW	3	5,465,212 (GRCm39)	missense	probably benign
R4819:Zfhx4	UTSW	3	5,468,974 (GRCm39)	missense	probably benign	0.05
R4937:Zfhx4	UTSW	3	5,307,071 (GRCm39)	missense	probably damaging	1.00
R4980:Zfhx4	UTSW	3	5,464,039 (GRCm39)	missense	possibly damaging	0.47
R5124:Zfhx4	UTSW	3	5,307,107 (GRCm39)	missense	probably damaging	1.00
R5214:Zfhx4	UTSW	3	5,468,701 (GRCm39)	missense	probably damaging	1.00
R5361:Zfhx4	UTSW	3	5,464,267 (GRCm39)	missense	probably damaging	0.99
R5375:Zfhx4	UTSW	3	5,477,485 (GRCm39)	missense	probably damaging	0.99
R5485:Zfhx4	UTSW	3	5,308,067 (GRCm39)	missense	probably damaging	1.00
R5588:Zfhx4	UTSW	3	5,468,198 (GRCm39)	missense	probably damaging	1.00
R5609:Zfhx4	UTSW	3	5,468,679 (GRCm39)	missense	probably damaging	1.00
R5726:Zfhx4	UTSW	3	5,468,381 (GRCm39)	missense	probably benign	0.02
R5758:Zfhx4	UTSW	3	5,467,680 (GRCm39)	missense	probably damaging	1.00
R5865:Zfhx4	UTSW	3	5,467,719 (GRCm39)	missense	probably damaging	1.00
R5938:Zfhx4	UTSW	3	5,467,198 (GRCm39)	missense	probably damaging	0.99
R5952:Zfhx4	UTSW	3	5,462,030 (GRCm39)	missense	probably damaging	0.99
R6043:Zfhx4	UTSW	3	5,468,487 (GRCm39)	missense	probably benign	0.00
R6045:Zfhx4	UTSW	3	5,462,019 (GRCm39)	missense	probably damaging	1.00
R6125:Zfhx4	UTSW	3	5,463,871 (GRCm39)	missense	possibly damaging	0.68
R6354:Zfhx4	UTSW	3	5,467,011 (GRCm39)	missense	probably benign
R6374:Zfhx4	UTSW	3	5,309,095 (GRCm39)	missense	probably damaging	1.00
R6378:Zfhx4	UTSW	3	5,308,410 (GRCm39)	missense	probably benign	0.07
R6380:Zfhx4	UTSW	3	5,478,170 (GRCm39)	missense	probably damaging	0.99
R6413:Zfhx4	UTSW	3	5,308,205 (GRCm39)	missense	probably damaging	1.00
R6449:Zfhx4	UTSW	3	5,307,488 (GRCm39)	missense	probably damaging	1.00
R6539:Zfhx4	UTSW	3	5,309,168 (GRCm39)	missense	probably damaging	0.99
R6714:Zfhx4	UTSW	3	5,306,897 (GRCm39)	missense	probably damaging	1.00
R6933:Zfhx4	UTSW	3	5,478,047 (GRCm39)	missense	probably damaging	0.99
R6982:Zfhx4	UTSW	3	5,468,890 (GRCm39)	missense	probably damaging	1.00
R7104:Zfhx4	UTSW	3	5,467,549 (GRCm39)	missense	probably damaging	0.97
R7127:Zfhx4	UTSW	3	5,478,104 (GRCm39)	missense	probably damaging	0.99
R7138:Zfhx4	UTSW	3	5,477,107 (GRCm39)	missense	possibly damaging	0.69
R7161:Zfhx4	UTSW	3	5,309,143 (GRCm39)	missense	possibly damaging	0.65
R7213:Zfhx4	UTSW	3	5,461,704 (GRCm39)	missense	probably benign
R7483:Zfhx4	UTSW	3	5,477,237 (GRCm39)	missense	probably damaging	0.98
R7514:Zfhx4	UTSW	3	5,307,267 (GRCm39)	missense	possibly damaging	0.91
R7544:Zfhx4	UTSW	3	5,477,875 (GRCm39)	missense	probably damaging	0.98
R7565:Zfhx4	UTSW	3	5,455,426 (GRCm39)	missense	probably benign	0.04
R7611:Zfhx4	UTSW	3	5,468,831 (GRCm39)	missense	probably damaging	1.00
R7640:Zfhx4	UTSW	3	5,477,540 (GRCm39)	missense	probably benign	0.19
R7649:Zfhx4	UTSW	3	5,307,170 (GRCm39)	missense	probably damaging	1.00
R7689:Zfhx4	UTSW	3	5,476,946 (GRCm39)	missense	probably benign	0.05
R7711:Zfhx4	UTSW	3	5,462,016 (GRCm39)	missense	probably damaging	0.98
R7895:Zfhx4	UTSW	3	5,307,259 (GRCm39)	missense	probably benign	0.00
R7920:Zfhx4	UTSW	3	5,465,515 (GRCm39)	missense	possibly damaging	0.62
R7972:Zfhx4	UTSW	3	5,477,533 (GRCm39)	missense	probably benign	0.02
R7993:Zfhx4	UTSW	3	5,478,047 (GRCm39)	missense	probably damaging	1.00
R8133:Zfhx4	UTSW	3	5,465,554 (GRCm39)	missense	probably damaging	0.99
R8158:Zfhx4	UTSW	3	5,464,010 (GRCm39)	nonsense	probably null
R8272:Zfhx4	UTSW	3	5,308,927 (GRCm39)	missense	probably damaging	0.99
R8285:Zfhx4	UTSW	3	5,466,916 (GRCm39)	missense	probably benign	0.17
R8321:Zfhx4	UTSW	3	5,466,187 (GRCm39)	missense	probably damaging	1.00
R8381:Zfhx4	UTSW	3	5,447,676 (GRCm39)	missense	probably benign	0.00
R8434:Zfhx4	UTSW	3	5,463,918 (GRCm39)	missense	probably damaging	0.99
R8466:Zfhx4	UTSW	3	5,307,762 (GRCm39)	missense	probably damaging	1.00
R8515:Zfhx4	UTSW	3	5,464,534 (GRCm39)	missense	probably benign	0.00
R8525:Zfhx4	UTSW	3	5,464,603 (GRCm39)	missense	probably damaging	1.00
R8743:Zfhx4	UTSW	3	5,309,084 (GRCm39)	missense	probably damaging	1.00
R8830:Zfhx4	UTSW	3	5,463,949 (GRCm39)	missense	probably damaging	1.00
R8839:Zfhx4	UTSW	3	5,466,915 (GRCm39)	missense	probably benign
R8856:Zfhx4	UTSW	3	5,455,484 (GRCm39)	missense	probably benign	0.45
R8900:Zfhx4	UTSW	3	5,463,924 (GRCm39)	missense	probably damaging	1.00
R8917:Zfhx4	UTSW	3	5,464,159 (GRCm39)	missense	probably damaging	1.00
R9101:Zfhx4	UTSW	3	5,477,198 (GRCm39)	missense	probably benign	0.10
R9126:Zfhx4	UTSW	3	5,394,589 (GRCm39)	missense	probably damaging	0.99
R9159:Zfhx4	UTSW	3	5,466,217 (GRCm39)	missense	probably damaging	1.00
R9159:Zfhx4	UTSW	3	5,464,312 (GRCm39)	missense	probably damaging	0.98
R9241:Zfhx4	UTSW	3	5,308,697 (GRCm39)	missense	probably damaging	1.00
R9295:Zfhx4	UTSW	3	5,394,525 (GRCm39)	missense	probably benign
R9376:Zfhx4	UTSW	3	5,465,395 (GRCm39)	missense	probably benign	0.04
R9376:Zfhx4	UTSW	3	5,306,833 (GRCm39)	missense	probably damaging	1.00
R9550:Zfhx4	UTSW	3	5,464,572 (GRCm39)	missense	probably damaging	1.00
R9680:Zfhx4	UTSW	3	5,465,656 (GRCm39)	missense	probably damaging	1.00
R9782:Zfhx4	UTSW	3	5,466,514 (GRCm39)	missense	probably benign	0.38
R9787:Zfhx4	UTSW	3	5,455,506 (GRCm39)	missense	possibly damaging	0.94
R9790:Zfhx4	UTSW	3	5,464,922 (GRCm39)	missense	probably damaging	1.00
R9791:Zfhx4	UTSW	3	5,464,922 (GRCm39)	missense	probably damaging	1.00
RF019:Zfhx4	UTSW	3	5,468,327 (GRCm39)	missense	probably benign	0.08
X0025:Zfhx4	UTSW	3	5,476,896 (GRCm39)	missense	probably damaging	0.99
X0026:Zfhx4	UTSW	3	5,477,398 (GRCm39)	missense	probably benign	0.00
X0028:Zfhx4	UTSW	3	5,468,327 (GRCm39)	missense	probably damaging	1.00
X0028:Zfhx4	UTSW	3	5,467,474 (GRCm39)	missense	probably benign	0.13
X0054:Zfhx4	UTSW	3	5,464,770 (GRCm39)	nonsense	probably null
Z1177:Zfhx4	UTSW	3	5,307,506 (GRCm39)	missense	probably damaging	1.00
Z1187:Zfhx4	UTSW	3	5,308,067 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGCCACTTATCATCTGAGTCTAGC -3'
(R):5'- AAAGTCCTTGCCACCAATGC -3'

Sequencing Primer
(F):5'- CATCTGAGTCTAGCAAGATGTCAG -3'
(R):5'- GCTATTAGTAACGTTGCCATTGC -3'

Posted On

2015-04-17