Mutagenetix > Incidental Mutation

Incidental Mutation 'R3907:Dcaf6'

310228

Institutional Source

Beutler Lab

Gene Symbol

Dcaf6

Ensembl Gene

ENSMUSG00000026571

Gene Name

DDB1 and CUL4 associated factor 6

Synonyms

PC326, 1200006M05Rik, Iqwd1

MMRRC Submission

040908-MU

Accession Numbers

Genbank: NM_028759; MGI: 1921356

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3907 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

165328698-165460475 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 165424380 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 58 (C58*)

Ref Sequence

ENSEMBL: ENSMUSP00000027856 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000027856]

AlphaFold

Q9DC22

Predicted Effect

probably null
Transcript: ENSMUST00000027856
AA Change: C58*

SMART Domains

Protein: ENSMUSP00000027856
Gene: ENSMUSG00000026571
AA Change: C58*

Domain	Start	End	E-Value	Type
WD40	40	79	5.77e-5	SMART
WD40	82	124	1.2e-2	SMART
WD40	130	170	2.15e-1	SMART
WD40	184	220	3.33e-1	SMART
WD40	238	281	6.66e-1	SMART
low complexity region	364	374	N/A	INTRINSIC
low complexity region	499	510	N/A	INTRINSIC
low complexity region	669	676	N/A	INTRINSIC
IQ	691	713	1.25e1	SMART
WD40	722	763	3.84e0	SMART
WD40	766	805	1.22e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000195686

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.5%
20x: 95.5%

Validation Efficiency

97% (56/58)

Allele List at MGI

All alleles(2) : Gene trapped(2)

Other mutations in this stock

Total: 61 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930402H24Rik	C	T	2: 130,736,576 (GRCm38)	A663T	probably damaging	Het
Adamts3	C	A	5: 89,861,355 (GRCm38)	G150C	probably damaging	Het
Ampd3	A	G	7: 110,793,670 (GRCm38)	D215G	possibly damaging	Het
Ank2	A	G	3: 127,016,898 (GRCm38)	L513P	probably damaging	Het
Apba1	T	C	19: 23,937,506 (GRCm38)	I690T	probably damaging	Het
Arid1a	T	C	4: 133,692,912 (GRCm38)		probably benign	Het
Arsi	G	A	18: 60,916,651 (GRCm38)	G202E	probably benign	Het
Asns	C	T	6: 7,682,270 (GRCm38)		probably null	Het
Aspg	T	A	12: 112,112,259 (GRCm38)	Y57*	probably null	Het
Asph	T	C	4: 9,474,934 (GRCm38)	K680R	probably benign	Het
Atp2b4	A	T	1: 133,738,586 (GRCm38)	S243T	probably damaging	Het
Cacna1s	T	A	1: 136,084,269 (GRCm38)	M483K	probably damaging	Het
Car4	G	A	11: 84,964,357 (GRCm38)	V141M	probably damaging	Het
Cct4	A	G	11: 23,001,560 (GRCm38)	I376V	probably benign	Het
Chrm4	C	T	2: 91,927,739 (GRCm38)	A164V	probably damaging	Het
Csf3r	A	T	4: 126,034,447 (GRCm38)	D291V	probably benign	Het
Ddi2	T	C	4: 141,684,281 (GRCm38)	D440G	probably benign	Het
Defb4	A	T	8: 19,201,261 (GRCm38)	Q48L	possibly damaging	Het
Duox2	C	T	2: 122,283,060 (GRCm38)		probably null	Het
E130308A19Rik	C	T	4: 59,752,393 (GRCm38)	T502I	probably benign	Het
Ephb1	A	G	9: 102,001,726 (GRCm38)	C522R	probably benign	Het
Fam76a	T	C	4: 132,916,121 (GRCm38)	K101E	probably damaging	Het
Fat1	G	C	8: 45,023,035 (GRCm38)	R1706T	probably benign	Het
Fn1	C	T	1: 71,607,913 (GRCm38)	G1482R	probably damaging	Het
Gm10110	T	C	14: 89,898,147 (GRCm38)		noncoding transcript	Het
Gphn	T	A	12: 78,493,942 (GRCm38)		probably benign	Het
Hars	A	T	18: 36,782,716 (GRCm38)	D48E	probably benign	Het
Hmgcll1	G	A	9: 76,072,661 (GRCm38)	R111H	probably benign	Het
Ighv3-4	A	G	12: 114,253,918 (GRCm38)	S18P	probably damaging	Het
Iws1	G	A	18: 32,079,920 (GRCm38)	E134K	possibly damaging	Het
Kcnj4	G	T	15: 79,485,745 (GRCm38)	H11Q	probably benign	Het
Krt16	A	G	11: 100,247,163 (GRCm38)	V329A	possibly damaging	Het
Loxhd1	A	T	18: 77,408,768 (GRCm38)	M1575L	possibly damaging	Het
Mapkapk2	A	T	1: 131,056,914 (GRCm38)	S234T	probably damaging	Het
Mum1	T	C	10: 80,238,316 (GRCm38)	V401A	probably damaging	Het
Mxd1	G	T	6: 86,650,960 (GRCm38)	Q199K	probably benign	Het
Nlrp5	T	A	7: 23,433,646 (GRCm38)	D905E	possibly damaging	Het
Olfr1222	A	C	2: 89,125,583 (GRCm38)	Y49*	probably null	Het
Olfr5	A	T	7: 6,480,679 (GRCm38)	V159D	probably damaging	Het
Otoa	A	T	7: 121,125,565 (GRCm38)	Q489L	probably damaging	Het
Pced1b	T	C	15: 97,384,550 (GRCm38)	S157P	probably damaging	Het
Ppp1r16b	T	C	2: 158,761,490 (GRCm38)	I345T	probably benign	Het
Prrt4	G	T	6: 29,177,174 (GRCm38)	L199M	probably damaging	Het
Ptpn6	T	C	6: 124,725,276 (GRCm38)	D347G	possibly damaging	Het
Rcan1	A	G	16: 92,466,029 (GRCm38)		probably benign	Het
Rif1	C	T	2: 52,112,545 (GRCm38)	L2004F	probably benign	Het
Rnf185	A	G	11: 3,426,681 (GRCm38)		probably benign	Het
Shank2	C	T	7: 144,409,576 (GRCm38)	P307L	probably damaging	Het
Slc19a3	G	A	1: 83,014,813 (GRCm38)	R396C	possibly damaging	Het
Stn1	T	C	19: 47,507,823 (GRCm38)	D321G	probably damaging	Het
Taar7a	T	C	10: 23,992,559 (GRCm38)	Y308C	probably benign	Het
Tespa1	T	C	10: 130,356,797 (GRCm38)		probably benign	Het
Tmcc2	T	C	1: 132,360,638 (GRCm38)	D359G	probably damaging	Het
Trhde	C	T	10: 114,800,696 (GRCm38)	G202E	possibly damaging	Het
Trip12	T	C	1: 84,732,106 (GRCm38)	T469A	possibly damaging	Het
Trip4	A	G	9: 65,833,426 (GRCm38)	I533T	probably benign	Het
Tsc22d1	T	C	14: 76,416,543 (GRCm38)	I154T	probably damaging	Het
Ttn	C	A	2: 76,903,342 (GRCm38)		probably benign	Het
Ugt8a	T	C	3: 125,914,982 (GRCm38)	T160A	possibly damaging	Het
Usp54	A	T	14: 20,586,113 (GRCm38)	S288T	probably damaging	Het
Utrn	C	T	10: 12,710,182 (GRCm38)		probably benign	Het

Other mutations in Dcaf6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00828:Dcaf6	APN	1	165,338,347 (GRCm38)	splice site	probably benign
IGL01377:Dcaf6	APN	1	165,388,724 (GRCm38)	missense	probably benign	0.01
IGL02027:Dcaf6	APN	1	165,424,341 (GRCm38)	missense	probably damaging	1.00
IGL02390:Dcaf6	APN	1	165,422,921 (GRCm38)	missense	possibly damaging	0.50
IGL02754:Dcaf6	APN	1	165,338,346 (GRCm38)	critical splice acceptor site	probably null
IGL02900:Dcaf6	APN	1	165,399,775 (GRCm38)	missense	probably damaging	1.00
IGL03119:Dcaf6	APN	1	165,339,976 (GRCm38)	missense	probably damaging	1.00
IGL03211:Dcaf6	APN	1	165,422,933 (GRCm38)	missense	possibly damaging	0.55
R0588:Dcaf6	UTSW	1	165,420,223 (GRCm38)	missense	possibly damaging	0.89
R1494:Dcaf6	UTSW	1	165,333,373 (GRCm38)	missense	probably damaging	0.99
R1512:Dcaf6	UTSW	1	165,352,020 (GRCm38)	missense	probably benign	0.22
R1840:Dcaf6	UTSW	1	165,399,748 (GRCm38)	missense	probably damaging	0.96
R2191:Dcaf6	UTSW	1	165,422,864 (GRCm38)	missense	probably benign	0.07
R2297:Dcaf6	UTSW	1	165,399,862 (GRCm38)	missense	probably damaging	1.00
R3082:Dcaf6	UTSW	1	165,422,852 (GRCm38)	splice site	probably benign
R3861:Dcaf6	UTSW	1	165,429,269 (GRCm38)	missense	probably damaging	1.00
R4521:Dcaf6	UTSW	1	165,390,490 (GRCm38)	missense	probably damaging	0.98
R4531:Dcaf6	UTSW	1	165,411,467 (GRCm38)	missense	probably damaging	1.00
R4906:Dcaf6	UTSW	1	165,411,463 (GRCm38)	critical splice donor site	probably null
R4916:Dcaf6	UTSW	1	165,420,205 (GRCm38)	missense	probably damaging	1.00
R4956:Dcaf6	UTSW	1	165,388,785 (GRCm38)	missense	probably benign	0.00
R5080:Dcaf6	UTSW	1	165,420,121 (GRCm38)	missense	probably damaging	1.00
R5091:Dcaf6	UTSW	1	165,330,003 (GRCm38)	missense	possibly damaging	0.76
R5277:Dcaf6	UTSW	1	165,424,346 (GRCm38)	missense	probably benign	0.09
R5512:Dcaf6	UTSW	1	165,399,835 (GRCm38)	missense	possibly damaging	0.84
R5914:Dcaf6	UTSW	1	165,351,155 (GRCm38)	missense	probably benign
R6004:Dcaf6	UTSW	1	165,388,685 (GRCm38)	missense	probably benign	0.00
R6239:Dcaf6	UTSW	1	165,351,270 (GRCm38)	missense	possibly damaging	0.47
R6736:Dcaf6	UTSW	1	165,399,785 (GRCm38)	missense	possibly damaging	0.77
R7051:Dcaf6	UTSW	1	165,424,317 (GRCm38)	missense	possibly damaging	0.82
R7110:Dcaf6	UTSW	1	165,351,968 (GRCm38)	missense	probably benign	0.22
R7583:Dcaf6	UTSW	1	165,333,310 (GRCm38)	missense	probably damaging	1.00
R7776:Dcaf6	UTSW	1	165,352,054 (GRCm38)	nonsense	probably null
R7790:Dcaf6	UTSW	1	165,399,715 (GRCm38)	missense	probably damaging	1.00
R8369:Dcaf6	UTSW	1	165,357,474 (GRCm38)	missense	probably damaging	1.00
R8411:Dcaf6	UTSW	1	165,388,675 (GRCm38)	missense	probably benign	0.03
R9061:Dcaf6	UTSW	1	165,336,763 (GRCm38)	missense	probably damaging	0.99
R9307:Dcaf6	UTSW	1	165,399,667 (GRCm38)	missense	possibly damaging	0.90
R9375:Dcaf6	UTSW	1	165,357,483 (GRCm38)	missense	probably damaging	1.00
R9626:Dcaf6	UTSW	1	165,399,695 (GRCm38)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TGTGGTACCTACATGTGACAATG -3'
(R):5'- ATGTGAATTGAGAACTGAAGTCTGG -3'

Sequencing Primer
(F):5'- ACAATTCCCTTTTTGCTAGC -3'
(R):5'- GAGAACTGAAGTCTGGTTTCAAC -3'

Posted On

2015-04-17