Mutagenetix > Incidental Mutation

Incidental Mutation 'R3894:Mslnl'

310471

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R3894 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

25736040-25748330 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 25742934 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Methionine at position 128 (V128M)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098]

AlphaFold

Q8C160

Predicted Effect

probably damaging
Transcript: ENSMUST00000047098
AA Change: V128M

PolyPhen 2 Score 0.971 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: V128M

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102319

Meta Mutation Damage Score

0.2148

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Akt1s1	A	G	7: 44,853,939 (GRCm38)	D180G	probably damaging	Het
Aldh1a7	A	T	19: 20,696,398 (GRCm38)	Y457*	probably null	Het
Alpk3	C	T	7: 81,078,390 (GRCm38)	P423S	possibly damaging	Het
Aox2	A	T	1: 58,334,678 (GRCm38)		probably null	Het
Crebbp	T	C	16: 4,096,102 (GRCm38)	T1316A	probably benign	Het
Cul3	C	A	1: 80,283,690 (GRCm38)	V273F	probably damaging	Het
Dnah1	G	T	14: 31,307,028 (GRCm38)	R582S	probably benign	Het
Fbxl2	T	C	9: 114,003,193 (GRCm38)	N51S	probably damaging	Het
Gapvd1	T	C	2: 34,728,476 (GRCm38)	D295G	probably benign	Het
Gdf7	A	G	12: 8,298,845 (GRCm38)	S151P	unknown	Het
Gm10277	T	C	11: 77,786,001 (GRCm38)		probably benign	Het
Hdac4	T	C	1: 91,970,968 (GRCm38)	E688G	possibly damaging	Het
Htr1d	A	G	4: 136,443,237 (GRCm38)	E259G	probably benign	Het
Ifi204	T	C	1: 173,749,208 (GRCm38)	H609R	possibly damaging	Het
Ift80	T	C	3: 68,917,999 (GRCm38)	D541G	probably damaging	Het
Il18r1	A	T	1: 40,474,874 (GRCm38)	H80L	possibly damaging	Het
Lrp4	G	A	2: 91,473,949 (GRCm38)	G158S	probably damaging	Het
Mesd	T	A	7: 83,897,785 (GRCm38)	L152H	probably damaging	Het
Morf4l1	A	T	9: 90,094,448 (GRCm38)	F276I	possibly damaging	Het
Mut	G	A	17: 40,955,139 (GRCm38)	C531Y	probably damaging	Het
Myo15	A	G	11: 60,504,319 (GRCm38)	T2480A	probably benign	Het
Olfr1284	C	A	2: 111,379,637 (GRCm38)	F212L	probably benign	Het
Olfr1317	T	C	2: 112,142,014 (GRCm38)	I23T	probably benign	Het
Olfr1387	G	A	11: 49,459,939 (GRCm38)	G87R	possibly damaging	Het
Olfr180	A	G	16: 58,916,339 (GRCm38)	F101L	probably benign	Het
Olfr437	A	T	6: 43,167,258 (GRCm38)	I67F	probably benign	Het
Ovgp1	A	G	3: 105,986,596 (GRCm38)		probably benign	Het
Ovgp1	T	C	3: 105,986,567 (GRCm38)		probably benign	Het
Pcbp4	A	T	9: 106,461,371 (GRCm38)	Q59L	possibly damaging	Het
Prg4	G	C	1: 150,454,759 (GRCm38)		probably benign	Het
Rad50	T	A	11: 53,678,870 (GRCm38)	I905L	probably benign	Het
Rp1l1	T	C	14: 64,029,307 (GRCm38)	S781P	probably benign	Het
Rps3	C	T	7: 99,479,896 (GRCm38)	R173H	probably benign	Het
Rtn3	T	A	19: 7,435,085 (GRCm38)	T86S	probably damaging	Het
Sdha	A	T	13: 74,334,391 (GRCm38)	S268T	probably benign	Het
Sgo2b	A	T	8: 63,928,733 (GRCm38)	V355E	possibly damaging	Het
Sh3glb2	T	C	2: 30,355,288 (GRCm38)	T60A	probably damaging	Het
Slc26a3	A	C	12: 31,464,720 (GRCm38)	Y513S	probably damaging	Het
Slc35b2	T	C	17: 45,566,442 (GRCm38)	V165A	probably benign	Het
Slco3a1	A	G	7: 74,284,613 (GRCm38)	W604R	probably damaging	Het
Tet2	A	G	3: 133,469,477 (GRCm38)	S1370P	possibly damaging	Het
Tmtc4	A	T	14: 122,921,319 (GRCm38)		probably null	Het
Tsga13	A	G	6: 30,912,263 (GRCm38)	V18A	probably benign	Het
Ugt2a3	T	C	5: 87,329,590 (GRCm38)	T317A	probably benign	Het
Zcchc4	A	T	5: 52,784,100 (GRCm38)	D79V	probably damaging	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25,743,667 (GRCm38)	unclassified	probably benign
IGL01629:Mslnl	APN	17	25,744,775 (GRCm38)	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25,746,151 (GRCm38)	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25,747,998 (GRCm38)	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25,744,103 (GRCm38)	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25,744,077 (GRCm38)	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25,743,203 (GRCm38)	nonsense	probably null
R0881:Mslnl	UTSW	17	25,742,965 (GRCm38)	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25,743,240 (GRCm38)	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25,743,240 (GRCm38)	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25,746,181 (GRCm38)	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25,746,181 (GRCm38)	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25,744,517 (GRCm38)	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25,744,969 (GRCm38)	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25,738,978 (GRCm38)	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25,738,968 (GRCm38)	nonsense	probably null
R5257:Mslnl	UTSW	17	25,746,165 (GRCm38)	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25,743,159 (GRCm38)	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25,737,842 (GRCm38)	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25,746,775 (GRCm38)	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25,737,902 (GRCm38)	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25,744,557 (GRCm38)	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25,746,073 (GRCm38)	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25,743,212 (GRCm38)	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25,743,210 (GRCm38)	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25,736,921 (GRCm38)	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25,743,183 (GRCm38)	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25,746,777 (GRCm38)	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25,746,988 (GRCm38)	missense	probably benign
R8735:Mslnl	UTSW	17	25,745,088 (GRCm38)	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25,745,073 (GRCm38)	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25,742,720 (GRCm38)	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25,742,532 (GRCm38)	intron	probably benign
RF007:Mslnl	UTSW	17	25,743,228 (GRCm38)	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- GCCATCAGAAGGGAGGTTTG -3'
(R):5'- CACAGGTCTGAAGCACTGAG -3'

Sequencing Primer
(F):5'- TTTGAACCCAGGACTGAGGTGAC -3'
(R):5'- GCCCTGCCGAGACAGTAG -3'

Posted On

2015-04-17