Mutagenetix > Incidental Mutation

Incidental Mutation 'R3896:Vmn1r86'

310543

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r86

Ensembl Gene

ENSMUSG00000070816

Gene Name

vomeronasal 1 receptor 86

Synonyms

Gm10301

MMRRC Submission

040807-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.049) question?

Stock #

R3896 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

12835924-12836874 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 12836093 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 261 (Y261C)

Ref Sequence

ENSEMBL: ENSMUSP00000154549 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094828] [ENSMUST00000226604] [ENSMUST00000227220] [ENSMUST00000227700]

AlphaFold

L7N213

Predicted Effect

probably benign
Transcript: ENSMUST00000094828
AA Change: Y261C

PolyPhen 2 Score 0.181 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000092423
Gene: ENSMUSG00000070816
AA Change: Y261C

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	306	1.1e-14	PFAM
Pfam:V1R	35	301	4e-31	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000210729

Predicted Effect

probably benign
Transcript: ENSMUST00000226604
AA Change: Y261C

PolyPhen 2 Score 0.181 (Sensitivity: 0.92; Specificity: 0.87)

Predicted Effect

probably benign
Transcript: ENSMUST00000227220
AA Change: Y261C

PolyPhen 2 Score 0.181 (Sensitivity: 0.92; Specificity: 0.87)

Predicted Effect

probably benign
Transcript: ENSMUST00000227700
AA Change: Y211C

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.4%
20x: 95.5%

Validation Efficiency

100% (48/48)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930503B20Rik	A	T	3: 146,356,868 (GRCm39)	N13K	possibly damaging	Het
Alas1	G	T	9: 106,119,000 (GRCm39)		probably null	Het
Arhgap20	A	T	9: 51,728,137 (GRCm39)	I117F	probably damaging	Het
Asz1	A	G	6: 18,075,766 (GRCm39)	I269T	probably benign	Het
Atp8a2	C	A	14: 60,263,589 (GRCm39)		probably null	Het
Atp8b2	A	T	3: 89,864,626 (GRCm39)	I163K	probably damaging	Het
Casd1	T	A	6: 4,640,980 (GRCm39)	F700L	probably damaging	Het
Ccdc80	A	G	16: 44,916,984 (GRCm39)	D580G	probably benign	Het
Cog7	C	T	7: 121,540,392 (GRCm39)		probably benign	Het
Cyp2c66	T	C	19: 39,130,722 (GRCm39)	V112A	possibly damaging	Het
D130040H23Rik	T	A	8: 69,755,610 (GRCm39)	C356S	probably damaging	Het
Emb	A	G	13: 117,409,598 (GRCm39)	*331W	probably null	Het
Enpp3	A	C	10: 24,653,847 (GRCm39)	S703R	possibly damaging	Het
Fam13b	T	C	18: 34,596,008 (GRCm39)		probably benign	Het
Foxp1	T	A	6: 99,052,897 (GRCm39)	Q97L	probably benign	Het
Gdf10	A	T	14: 33,656,438 (GRCm39)	N467Y	probably damaging	Het
Gm29394	C	T	15: 57,912,024 (GRCm39)		probably benign	Het
Gsn	T	C	2: 35,192,650 (GRCm39)	S522P	possibly damaging	Het
Hydin	T	A	8: 111,235,711 (GRCm39)	F1899I	possibly damaging	Het
Ints1	C	A	5: 139,743,399 (GRCm39)	E1658*	probably null	Het
Jakmip2	A	T	18: 43,682,751 (GRCm39)	F691Y	probably benign	Het
Klhl28	A	G	12: 65,004,333 (GRCm39)	F60S	probably damaging	Het
Loxhd1	C	T	18: 77,469,719 (GRCm39)	S992L	possibly damaging	Het
Lrp1b	A	T	2: 40,812,440 (GRCm39)		probably null	Het
Macf1	A	T	4: 123,364,987 (GRCm39)	I3258N	possibly damaging	Het
Map4k2	G	T	19: 6,391,958 (GRCm39)	E91*	probably null	Het
Matcap1	T	A	8: 106,009,920 (GRCm39)	H343L	probably benign	Het
Myo1b	A	T	1: 51,812,420 (GRCm39)	V739E	probably damaging	Het
Naa35	C	T	13: 59,755,109 (GRCm39)	T185I	probably damaging	Het
Or10v9	T	C	19: 11,832,951 (GRCm39)	D122G	probably damaging	Het
Or4c15b	A	G	2: 89,113,441 (GRCm39)	F33S	possibly damaging	Het
Reg4	A	T	3: 98,132,082 (GRCm39)		probably benign	Het
Rnaseh2b	A	C	14: 62,597,906 (GRCm39)		probably benign	Het
Rnf123	G	A	9: 107,946,302 (GRCm39)		probably benign	Het
Scn8a	A	T	15: 100,933,379 (GRCm39)	M1528L	probably benign	Het
Sdr16c5	T	A	4: 4,006,609 (GRCm39)	T228S	probably damaging	Het
Sgo2a	T	C	1: 58,052,805 (GRCm39)	C202R	probably damaging	Het
Slc25a46	A	G	18: 31,716,725 (GRCm39)	L259P	probably damaging	Het
Slc4a4	A	G	5: 89,345,625 (GRCm39)		probably benign	Het
Sox14	G	T	9: 99,757,636 (GRCm39)	H34Q	probably damaging	Het
Syna	T	A	5: 134,587,165 (GRCm39)	K595*	probably null	Het
Taf4	A	G	2: 179,573,807 (GRCm39)	V687A	probably benign	Het
Tmbim7	C	T	5: 3,711,916 (GRCm39)	H54Y	probably benign	Het
Vmn1r212	T	C	13: 23,068,067 (GRCm39)	M89V	probably benign	Het
Xkr4	A	G	1: 3,286,414 (GRCm39)	I592T	probably damaging	Het
Ywhah	A	G	5: 33,184,349 (GRCm39)	Y184C	probably damaging	Het
Zkscan16	C	T	4: 58,946,125 (GRCm39)		probably benign	Het

Other mutations in Vmn1r86

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00492:Vmn1r86	APN	7	12,836,468 (GRCm39)	missense	possibly damaging	0.91
IGL01124:Vmn1r86	APN	7	12,836,856 (GRCm39)	missense	probably benign	0.04
IGL01291:Vmn1r86	APN	7	12,836,433 (GRCm39)	missense	probably benign	0.14
IGL02347:Vmn1r86	APN	7	12,836,574 (GRCm39)	missense	probably damaging	0.99
IGL02457:Vmn1r86	APN	7	12,836,707 (GRCm39)	missense	probably benign
IGL02983:Vmn1r86	APN	7	12,836,741 (GRCm39)	missense	probably damaging	1.00
ANU05:Vmn1r86	UTSW	7	12,836,433 (GRCm39)	missense	probably benign	0.14
R0304:Vmn1r86	UTSW	7	12,836,707 (GRCm39)	missense	probably benign
R0525:Vmn1r86	UTSW	7	12,836,088 (GRCm39)	missense	probably benign	0.13
R1958:Vmn1r86	UTSW	7	12,836,621 (GRCm39)	missense	possibly damaging	0.94
R2227:Vmn1r86	UTSW	7	12,836,847 (GRCm39)	missense	probably benign	0.00
R3149:Vmn1r86	UTSW	7	12,836,358 (GRCm39)	nonsense	probably null
R4735:Vmn1r86	UTSW	7	12,836,221 (GRCm39)	missense	probably damaging	1.00
R5219:Vmn1r86	UTSW	7	12,836,382 (GRCm39)	missense	probably damaging	1.00
R6003:Vmn1r86	UTSW	7	12,836,125 (GRCm39)	nonsense	probably null
R6457:Vmn1r86	UTSW	7	12,836,279 (GRCm39)	missense	possibly damaging	0.70
R7442:Vmn1r86	UTSW	7	12,835,983 (GRCm39)	missense	possibly damaging	0.70
R7622:Vmn1r86	UTSW	7	12,836,685 (GRCm39)	missense	probably benign	0.07
R8436:Vmn1r86	UTSW	7	12,836,771 (GRCm39)	missense	probably benign	0.25
R9141:Vmn1r86	UTSW	7	12,836,789 (GRCm39)	nonsense	probably null
R9374:Vmn1r86	UTSW	7	12,836,781 (GRCm39)	missense	possibly damaging	0.86
R9398:Vmn1r86	UTSW	7	12,836,261 (GRCm39)	missense	probably damaging	0.98
R9551:Vmn1r86	UTSW	7	12,836,781 (GRCm39)	missense	possibly damaging	0.86
R9552:Vmn1r86	UTSW	7	12,836,781 (GRCm39)	missense	possibly damaging	0.86
R9606:Vmn1r86	UTSW	7	12,836,741 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGGTTTCAGTTCAGCCATG -3'
(R):5'- TCACTCATGATCTCATCTATGTGG -3'

Sequencing Primer
(F):5'- TCAGTTCAGCCATGGAGTGAAC -3'
(R):5'- ATGATGTGGGCCAGCCTCTAC -3'

Posted On

2015-04-17