Mutagenetix > Incidental Mutation

Incidental Mutation 'R3977:Tas2r103'

311071

Institutional Source

Beutler Lab

Gene Symbol

Tas2r103

Ensembl Gene

ENSMUSG00000030196

Gene Name

taste receptor, type 2, member 103

Synonyms

mt2r63, T2R3, Tas2r10, EG667992, mGR03, TRB2, Tas2r3

MMRRC Submission

040940-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.052) question?

Stock #

R3977 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

133036163-133037101 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 133036317 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 262 (L262P)

Ref Sequence

ENSEMBL: ENSMUSP00000032317 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032317]

AlphaFold

Q9JKA3

Predicted Effect

probably benign
Transcript: ENSMUST00000032317
AA Change: L262P

PolyPhen 2 Score 0.161 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000032317
Gene: ENSMUSG00000030196
AA Change: L262P

Domain	Start	End	E-Value	Type
Pfam:TAS2R	5	301	1.5e-79	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 96.9%
20x: 93.7%

Validation Efficiency

96% (50/52)

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ace	C	T	11: 105,981,838	P942L	possibly damaging	Het
Adam28	A	G	14: 68,610,994	V671A	probably benign	Het
Arfgef1	A	G	1: 10,209,634	V236A	probably benign	Het
B020004C17Rik	G	T	14: 57,017,188	M156I	possibly damaging	Het
Bicral	T	A	17: 46,830,991	M1L	unknown	Het
Brpf3	A	G	17: 28,807,042	E363G	possibly damaging	Het
Ccdc96	T	C	5: 36,485,166	L172P	possibly damaging	Het
Cln3	G	A	7: 126,580,136		probably benign	Het
Dnah7c	T	C	1: 46,628,911	I1526T	possibly damaging	Het
Fmo3	T	G	1: 162,958,578	E281A	probably damaging	Het
Frem2	A	G	3: 53,652,070	I1672T	probably benign	Het
Gbp2b	A	G	3: 142,603,709	I194V	probably benign	Het
Gprc6a	C	A	10: 51,621,101	V449L	probably benign	Het
Hk1	A	G	10: 62,290,319	V396A	probably benign	Het
Hoxc13	T	C	15: 102,921,240	V18A	possibly damaging	Het
Hr	A	G	14: 70,563,584	T699A	probably benign	Het
Il19	A	T	1: 130,936,033	C74S	probably damaging	Het
Krt2	T	C	15: 101,811,127	T703A	unknown	Het
Lrig2	A	T	3: 104,457,844	V664E	probably damaging	Het
Lrrc37a	T	C	11: 103,457,604	K2755R	unknown	Het
Man1c1	G	C	4: 134,703,438	P11R	probably damaging	Het
Mbtd1	A	G	11: 93,905,175	N13D	probably benign	Het
Mfsd4b5	T	C	10: 39,974,708		probably benign	Het
Nras	A	G	3: 103,060,225	I46V	probably benign	Het
Oasl1	C	T	5: 114,932,898	T274I	probably damaging	Het
Ogfod2	C	A	5: 124,113,209		probably null	Het
Olfr1264	C	A	2: 90,021,745	G107V	probably damaging	Het
Olfr1369-ps1	T	A	13: 21,115,861	H56Q	probably benign	Het
Olfr1419	C	A	19: 11,870,505	R237L	possibly damaging	Het
Olfr1420	T	A	19: 11,896,516	F165Y	probably damaging	Het
Olfr774	C	T	10: 129,238,508	R120C	probably damaging	Het
Olfr96	T	C	17: 37,225,158	V11A	probably benign	Het
Pkmyt1	A	G	17: 23,735,331	M362V	probably benign	Het
Ppfia2	A	G	10: 106,830,629	T399A	possibly damaging	Het
Ppp1r12b	A	G	1: 134,765,975	S983P	probably benign	Het
Ptpn22	A	G	3: 103,873,641		probably benign	Het
Raph1	A	T	1: 60,498,523	D491E	probably benign	Het
Rc3h1	A	G	1: 160,959,399		probably null	Het
Rtn4	T	C	11: 29,693,819	L5P	probably benign	Het
Sdccag3	T	A	2: 26,384,793	N364I	probably damaging	Het
Slc23a4	C	T	6: 34,953,788	V400I	probably benign	Het
Slco1a5	A	G	6: 142,258,972		probably benign	Het
Smpd1	T	C	7: 105,555,901	F329S	probably benign	Het
Sycp2l	A	G	13: 41,141,964	I334M	probably damaging	Het
Ten1	G	A	11: 116,216,945		probably null	Het
Tfap2d	C	G	1: 19,104,494	S57C	possibly damaging	Het
Tnr	A	G	1: 159,892,023	M957V	probably benign	Het
Trbv13-3	A	G	6: 41,130,145		probably benign	Het
Trp53rkb	C	T	2: 166,795,526	A134V	possibly damaging	Het
Trp63	A	G	16: 25,820,740		probably benign	Het
Vmn2r112	T	A	17: 22,603,115	V258E	probably damaging	Het
Vmn2r74	T	C	7: 85,958,137	Y126C	probably benign	Het
Vmn2r96	T	A	17: 18,597,679	I698N	probably damaging	Het

Other mutations in Tas2r103

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02230:Tas2r103	APN	6	133037056	missense	possibly damaging	0.86
IGL02332:Tas2r103	APN	6	133036512	missense	probably benign	0.21
IGL03122:Tas2r103	APN	6	133036909	missense	probably damaging	1.00
IGL03167:Tas2r103	APN	6	133036660	missense	probably damaging	0.98
R0380:Tas2r103	UTSW	6	133036203	missense	probably damaging	1.00
R0671:Tas2r103	UTSW	6	133036350	missense	probably benign	0.01
R1700:Tas2r103	UTSW	6	133036811	missense	probably damaging	1.00
R2098:Tas2r103	UTSW	6	133036597	missense	probably benign
R3979:Tas2r103	UTSW	6	133036317	missense	probably benign	0.16
R3980:Tas2r103	UTSW	6	133036317	missense	probably benign	0.16
R4924:Tas2r103	UTSW	6	133036198	missense	probably benign	0.02
R5779:Tas2r103	UTSW	6	133036945	missense	probably benign	0.02
R5839:Tas2r103	UTSW	6	133036816	missense	probably benign	0.06
R6265:Tas2r103	UTSW	6	133036531	missense	probably damaging	1.00
R7768:Tas2r103	UTSW	6	133036849	missense	probably benign	0.06
R8851:Tas2r103	UTSW	6	133036933	missense
R9435:Tas2r103	UTSW	6	133036723	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ATCCCTGATATGATTTAGGGGC -3'
(R):5'- AGCACTGGGTTTACACTCACC -3'

Sequencing Primer
(F):5'- ATGATTTAGGGGCCCCAATTC -3'
(R):5'- CATTCGCTGTGTCTTTGACAATG -3'

Posted On

2015-04-29