Mutagenetix > Incidental Mutation

Incidental Mutation 'R3979:Olfr1369-ps1'

311202

Institutional Source

Beutler Lab

Gene Symbol

Olfr1369-ps1

Ensembl Gene

ENSMUSG00000060404

Gene Name

olfactory receptor 1369, pseudogene 1

Synonyms

GA_x6K02T2QHY8-12126170-12125935, MOR256-31

MMRRC Submission

040942-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.272) question?

Stock #

R3979 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

21111483-21119095 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 21115861 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 56 (H56Q)

Ref Sequence

ENSEMBL: ENSMUSP00000151041 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079050] [ENSMUST00000213326] [ENSMUST00000213922] [ENSMUST00000215207] [ENSMUST00000215941]

AlphaFold

A0A140T8K7

Predicted Effect

probably benign
Transcript: ENSMUST00000079050
AA Change: H56Q

PolyPhen 2 Score 0.033 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000078059
Gene: ENSMUSG00000060404
AA Change: H56Q

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	6.2e-47	PFAM
Pfam:7tm_1	41	290	1.1e-30	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000213326
AA Change: H56Q

PolyPhen 2 Score 0.033 (Sensitivity: 0.95; Specificity: 0.82)

Predicted Effect

probably benign
Transcript: ENSMUST00000213922
AA Change: H56Q

PolyPhen 2 Score 0.033 (Sensitivity: 0.95; Specificity: 0.82)

Predicted Effect

probably benign
Transcript: ENSMUST00000215207
AA Change: H56Q

PolyPhen 2 Score 0.033 (Sensitivity: 0.95; Specificity: 0.82)

Predicted Effect

probably benign
Transcript: ENSMUST00000215941
AA Change: H56Q

PolyPhen 2 Score 0.033 (Sensitivity: 0.95; Specificity: 0.82)

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.2%
20x: 94.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb11	A	G	2: 69,323,976	V82A	probably benign	Het
Adam28	A	G	14: 68,610,994	V671A	probably benign	Het
Ak3	T	A	19: 29,047,718	S38C	probably damaging	Het
Arfgef1	A	G	1: 10,209,634	V236A	probably benign	Het
Arhgap10	T	C	8: 77,420,725	N170S	probably benign	Het
B020004C17Rik	G	T	14: 57,017,188	M156I	possibly damaging	Het
Bicral	T	A	17: 46,830,991	M1L	unknown	Het
Bop1	A	G	15: 76,453,876	L598P	probably damaging	Het
Cachd1	A	T	4: 100,970,888	D611V	probably damaging	Het
Cfap70	T	A	14: 20,439,719	E246D	probably benign	Het
Chl1	T	A	6: 103,715,284	Y294*	probably null	Het
Chrna2	T	A	14: 66,148,953	Y183N	probably damaging	Het
Dab2	A	G	15: 6,435,163		probably null	Het
Dnajb6	T	C	5: 29,751,008	F46L	possibly damaging	Het
Exoc7	T	C	11: 116,296,762	E275G	probably benign	Het
Fam208a	T	C	14: 27,477,130	L1335S	possibly damaging	Het
Frem2	A	G	3: 53,652,070	I1672T	probably benign	Het
Gm12166	T	A	11: 46,052,026	K90M	probably damaging	Het
Gprc6a	C	A	10: 51,621,101	V449L	probably benign	Het
H2-M10.4	A	G	17: 36,461,985	V35A	probably benign	Het
Hr	A	G	14: 70,563,584	T699A	probably benign	Het
Iffo1	A	G	6: 125,160,589		probably benign	Het
Iqgap1	G	A	7: 80,759,934	H218Y	probably damaging	Het
Itpr3	A	C	17: 27,085,131	K109Q	probably benign	Het
Itpr3	A	G	17: 27,091,572	D443G	probably damaging	Het
Katna1	T	C	10: 7,752,754	M249T	probably damaging	Het
Klk1b4	G	A	7: 44,211,593	G220D	probably damaging	Het
Krt24	A	G	11: 99,282,770	C242R	probably benign	Het
Madd	G	A	2: 91,176,828	T313I	possibly damaging	Het
Man1c1	G	C	4: 134,703,438	P11R	probably damaging	Het
Micalcl	A	G	7: 112,407,678		probably null	Het
Neil3	T	C	8: 53,623,664	T79A	probably damaging	Het
Nras	A	G	3: 103,060,225	I46V	probably benign	Het
Olfr1196	T	C	2: 88,700,448	S294G	probably benign	Het
Ppfia2	A	G	10: 106,830,629	T399A	possibly damaging	Het
Rarres1	A	G	3: 67,495,810	V86A	probably benign	Het
Rdh19	A	T	10: 127,850,075	R19W	possibly damaging	Het
Rock2	A	G	12: 16,972,736	K1059E	probably damaging	Het
Sparcl1	T	C	5: 104,092,781	H259R	probably benign	Het
Spata31d1c	A	G	13: 65,035,160	D172G	possibly damaging	Het
Stab2	T	C	10: 86,863,456	D515G	possibly damaging	Het
Sycp2l	A	G	13: 41,141,964	I334M	probably damaging	Het
Tas2r103	A	G	6: 133,036,317	L262P	probably benign	Het
Tcaf2	A	G	6: 42,642,547	V182A	probably damaging	Het
Tcof1	C	T	18: 60,831,533	E674K	possibly damaging	Het
Trp63	A	G	16: 25,820,740		probably benign	Het
Ttn	A	T	2: 76,745,394	W25052R	probably damaging	Het
Ubr1	T	A	2: 120,862,687	N1746I	probably benign	Het
Vax2	T	C	6: 83,737,547	V148A	probably damaging	Het
Vmn2r112	T	A	17: 22,603,115	V258E	probably damaging	Het
Vmn2r96	T	A	17: 18,597,679	I698N	probably damaging	Het
Wdr90	A	G	17: 25,859,278	V372A	probably benign	Het
Zfp335	C	T	2: 164,910,638	G62D	probably benign	Het
Zfp563	G	A	17: 33,105,727	R432H	probably benign	Het
Zhx1	T	C	15: 58,053,240	T537A	probably benign	Het

Other mutations in Olfr1369-ps1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01689:Olfr1369-ps1	APN	13	21116073	missense	probably damaging	0.97
R0631:Olfr1369-ps1	UTSW	13	21115908	missense	probably damaging	1.00
R0960:Olfr1369-ps1	UTSW	13	21116265	missense	possibly damaging	0.94
R1499:Olfr1369-ps1	UTSW	13	21116133	missense	probably benign	0.32
R1549:Olfr1369-ps1	UTSW	13	21116118	missense	probably benign	0.01
R1698:Olfr1369-ps1	UTSW	13	21116565	missense	probably benign	0.11
R1711:Olfr1369-ps1	UTSW	13	21116306	missense	probably benign	0.01
R2404:Olfr1369-ps1	UTSW	13	21115842	missense	probably damaging	1.00
R2471:Olfr1369-ps1	UTSW	13	21116429	missense	probably damaging	1.00
R3844:Olfr1369-ps1	UTSW	13	21116063	missense	possibly damaging	0.91
R3977:Olfr1369-ps1	UTSW	13	21115861	missense	probably benign	0.03
R4804:Olfr1369-ps1	UTSW	13	21116005	nonsense	probably null
R4914:Olfr1369-ps1	UTSW	13	21116397	missense	probably benign	0.12
R5210:Olfr1369-ps1	UTSW	13	21116052	missense	probably damaging	0.99
R5359:Olfr1369-ps1	UTSW	13	21116267	missense	probably damaging	1.00
R5700:Olfr1369-ps1	UTSW	13	21116001	missense	probably damaging	1.00
R6218:Olfr1369-ps1	UTSW	13	21116231	missense	probably damaging	1.00
R6767:Olfr1369-ps1	UTSW	13	21116057	missense	probably benign	0.02
R7396:Olfr1369-ps1	UTSW	13	21116307	missense	probably benign	0.02
R7476:Olfr1369-ps1	UTSW	13	21116021	missense	probably benign	0.04
R7612:Olfr1369-ps1	UTSW	13	21116047	missense	probably damaging	0.99
R8257:Olfr1369-ps1	UTSW	13	21116373	missense	probably benign	0.11
R9388:Olfr1369-ps1	UTSW	13	21116604	missense	probably damaging	0.96
R9697:Olfr1369-ps1	UTSW	13	21115722	missense	probably benign	0.21
V8831:Olfr1369-ps1	UTSW	13	21116003	missense	possibly damaging	0.93
Z1176:Olfr1369-ps1	UTSW	13	21116601	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGAAAGACTATGGACCCAAGC -3'
(R):5'- TGCAAGGGCTTACAAATAGCTG -3'

Sequencing Primer
(F):5'- GGACCCAAGCAATTACAGTACTTTG -3'
(R):5'- GGCTTACAAATAGCTGTGAAACGATC -3'

Posted On

2015-04-29