Mutagenetix > Incidental Mutation

Incidental Mutation 'R3980:Tas2r103'

311247

Institutional Source

Beutler Lab

Gene Symbol

Tas2r103

Ensembl Gene

ENSMUSG00000030196

Gene Name

taste receptor, type 2, member 103

Synonyms

mt2r63, T2R3, Tas2r10, EG667992, mGR03, TRB2, Tas2r3

MMRRC Submission

040843-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.050) question?

Stock #

R3980 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

133036163-133037101 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 133036317 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 262 (L262P)

Ref Sequence

ENSEMBL: ENSMUSP00000032317 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032317]

AlphaFold

Q9JKA3

Predicted Effect

probably benign
Transcript: ENSMUST00000032317
AA Change: L262P

PolyPhen 2 Score 0.161 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000032317
Gene: ENSMUSG00000030196
AA Change: L262P

Domain	Start	End	E-Value	Type
Pfam:TAS2R	5	301	1.5e-79	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.2%
20x: 94.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrb1	G	A	15: 74,582,943	G268R	probably damaging	Het
Amacr	T	A	15: 10,988,929	Y240*	probably null	Het
Ankhd1	A	T	18: 36,647,613	H1906L	probably damaging	Het
Arhgef25	C	A	10: 127,187,220	C106F	probably damaging	Het
Bean1	A	T	8: 104,211,098	Q103L	possibly damaging	Het
Ccr9	A	T	9: 123,779,376	N41I	probably benign	Het
Ceacam16	A	G	7: 19,858,633	F117L	probably benign	Het
Col4a1	T	C	8: 11,239,155		probably benign	Het
Csk	A	G	9: 57,630,780	Y48H	probably damaging	Het
Csmd1	T	A	8: 15,906,056	K3384*	probably null	Het
Ctnnd2	T	A	15: 30,669,443	H399Q	probably benign	Het
Cutal	T	C	2: 34,882,313	Y30H	possibly damaging	Het
Cybb	T	C	X: 9,444,588	Y425C	probably damaging	Het
Dab2	A	G	15: 6,435,163		probably null	Het
Defb30	C	A	14: 63,035,972	C64F	probably damaging	Het
Eif2a	T	C	3: 58,539,539	I45T	probably benign	Het
Esyt1	T	A	10: 128,511,524	D1044V	probably damaging	Het
Fam196b	G	A	11: 34,402,678	C240Y	probably benign	Het
Gabpb2	A	T	3: 95,188,770	V382E	probably damaging	Het
Glb1	T	A	9: 114,417,064	I61K	probably damaging	Het
Kcnt1	T	C	2: 25,893,214	V263A	possibly damaging	Het
Kdm5b	A	G	1: 134,619,670	D1019G	probably benign	Het
Klhl2	A	T	8: 64,743,075	L545M	probably damaging	Het
Klhl2	C	A	8: 64,743,081	G543C	probably damaging	Het
Krt31	G	T	11: 100,048,204	Q264K	probably damaging	Het
Lmnb1	A	G	18: 56,731,019	D232G	probably damaging	Het
Loxhd1	T	C	18: 77,414,159	F859L	probably damaging	Het
Map7	C	A	10: 20,267,353	T416K	unknown	Het
Med18	T	A	4: 132,462,940	I45F	probably benign	Het
Mn1	A	T	5: 111,421,770	H1202L	possibly damaging	Het
Mpp7	T	C	18: 7,444,062	D120G	probably benign	Het
Nlrp1b	A	T	11: 71,181,611	F469I	possibly damaging	Het
Nos3	T	A	5: 24,377,931	D685E	probably damaging	Het
Nrap	G	A	19: 56,381,552	A206V	probably benign	Het
Nuggc	T	C	14: 65,619,093		probably null	Het
Oasl1	C	T	5: 114,932,898	T274I	probably damaging	Het
Olfr1335	T	A	4: 118,809,303	Y187F	probably benign	Het
Olfr559	T	C	7: 102,723,752	N246S	probably damaging	Het
Parp3	T	C	9: 106,474,068	D278G	probably damaging	Het
Phc3	T	A	3: 30,936,931	Q346L	probably damaging	Het
Pigo	A	G	4: 43,019,231	L1029P	probably damaging	Het
Plcb3	A	G	19: 6,966,435	I66T	probably damaging	Het
Plch2	T	C	4: 154,984,798	S1019G	probably benign	Het
Plekhg6	C	A	6: 125,373,183	C264F	probably damaging	Het
Plxna2	C	T	1: 194,749,317	S538F	probably damaging	Het
Pou4f2	G	T	8: 78,435,438	H179N	possibly damaging	Het
Prpf39	T	C	12: 65,061,457		probably benign	Het
Rapgef1	G	A	2: 29,719,650	V700I	probably benign	Het
Rdh14	A	G	12: 10,394,703	I185V	probably benign	Het
Rnf111	A	T	9: 70,442,325	H785Q	probably damaging	Het
Rttn	C	T	18: 89,017,275	R758W	probably benign	Het
Sik3	G	A	9: 46,202,063	V601M	probably damaging	Het
Slc22a14	T	C	9: 119,178,486	T286A	probably benign	Het
Slc36a3	G	A	11: 55,135,383	T203I	probably benign	Het
Spata31	A	T	13: 64,922,654	Q872L	probably benign	Het
Spata31d1c	A	G	13: 65,035,160	D172G	possibly damaging	Het
Sphkap	A	T	1: 83,267,494		probably null	Het
Stat6	T	C	10: 127,655,379	V463A	probably damaging	Het
Stx7	T	C	10: 24,185,049	S225P	probably damaging	Het
Sult2a7	A	T	7: 14,473,409		probably benign	Het
Tada2a	A	T	11: 84,103,120	F179L	probably benign	Het
Tfap2d	A	G	1: 19,165,963	I382V	possibly damaging	Het
Tshr	C	A	12: 91,537,743	A485D	probably damaging	Het
Vmn1r32	T	A	6: 66,553,714	Y26F	probably damaging	Het
Vmn1r5	A	G	6: 56,985,651	T104A	probably damaging	Het
Wbp1l	T	A	19: 46,653,957		probably null	Het

Other mutations in Tas2r103

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02230:Tas2r103	APN	6	133037056	missense	possibly damaging	0.86
IGL02332:Tas2r103	APN	6	133036512	missense	probably benign	0.21
IGL03122:Tas2r103	APN	6	133036909	missense	probably damaging	1.00
IGL03167:Tas2r103	APN	6	133036660	missense	probably damaging	0.98
R0380:Tas2r103	UTSW	6	133036203	missense	probably damaging	1.00
R0671:Tas2r103	UTSW	6	133036350	missense	probably benign	0.01
R1700:Tas2r103	UTSW	6	133036811	missense	probably damaging	1.00
R2098:Tas2r103	UTSW	6	133036597	missense	probably benign
R3977:Tas2r103	UTSW	6	133036317	missense	probably benign	0.16
R3979:Tas2r103	UTSW	6	133036317	missense	probably benign	0.16
R4924:Tas2r103	UTSW	6	133036198	missense	probably benign	0.02
R5779:Tas2r103	UTSW	6	133036945	missense	probably benign	0.02
R5839:Tas2r103	UTSW	6	133036816	missense	probably benign	0.06
R6265:Tas2r103	UTSW	6	133036531	missense	probably damaging	1.00
R7768:Tas2r103	UTSW	6	133036849	missense	probably benign	0.06
R8851:Tas2r103	UTSW	6	133036933	missense
R9435:Tas2r103	UTSW	6	133036723	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GGATCCCTGATATGATTTAGGGGC -3'
(R):5'- GGGTTTACACTCACCCCATTCG -3'

Sequencing Primer
(F):5'- ATGATTTAGGGGCCCCAATTC -3'
(R):5'- CATTCGCTGTGTCTTTGACAATG -3'

Posted On

2015-04-29