Mutagenetix > Incidental Mutation

Incidental Mutation 'R4013:Tas2r116'

311815

Institutional Source

Beutler Lab

Gene Symbol

Tas2r116

Ensembl Gene

ENSMUSG00000030194

Gene Name

taste receptor, type 2, member 116

Synonyms

mGR16, TRB1, Tas2r7, Tas2r16, T2R16, mt2r56, Tas2r14, TRB4

MMRRC Submission

040950-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4013 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

132832401-132833318 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 132833230 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Arginine at position 277 (H277R)

Ref Sequence

ENSEMBL: ENSMUSP00000032315 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032315]

AlphaFold

Q7M713

Predicted Effect

probably damaging
Transcript: ENSMUST00000032315
AA Change: H277R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000032315
Gene: ENSMUSG00000030194
AA Change: H277R

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	300	2.8e-89	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000203358

Meta Mutation Damage Score

0.7311

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.6%
20x: 92.4%

Validation Efficiency

98% (47/48)

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc6	T	C	7: 45,668,104 (GRCm39)	Q168R	probably benign	Het
Adgrg3	A	G	8: 95,761,727 (GRCm39)		probably benign	Het
Apold1	A	G	6: 134,960,869 (GRCm39)	I108V	probably benign	Het
Atp6v0a2	G	A	5: 124,789,860 (GRCm39)	V429M	probably damaging	Het
Cbln4	A	T	2: 171,879,477 (GRCm39)	M137K	probably damaging	Het
Cfap57	A	G	4: 118,450,340 (GRCm39)	V594A	probably benign	Het
Chd9	A	G	8: 91,699,797 (GRCm39)	E28G	possibly damaging	Het
Clip4	T	A	17: 72,163,541 (GRCm39)	C704*	probably null	Het
Col8a2	T	A	4: 126,204,908 (GRCm39)		probably benign	Het
Cyp3a59	A	G	5: 146,016,193 (GRCm39)	T17A	probably benign	Het
Cyp4f14	G	A	17: 33,135,853 (GRCm39)	Q3*	probably null	Het
Cysltr2	A	G	14: 73,267,005 (GRCm39)	I235T	probably damaging	Het
Esp34	C	A	17: 38,870,446 (GRCm39)	C45*	probably null	Het
Gabrg2	T	C	11: 41,862,707 (GRCm39)	K126E	possibly damaging	Het
Gm4846	A	C	1: 166,322,249 (GRCm39)		probably null	Het
Igsf21	T	C	4: 139,764,780 (GRCm39)	N165S	possibly damaging	Het
Kcnf1	A	G	12: 17,225,994 (GRCm39)	F76L	probably benign	Het
Kcns1	A	G	2: 164,010,177 (GRCm39)	V194A	probably damaging	Het
Kdm5a	T	A	6: 120,371,067 (GRCm39)	Y504N	probably damaging	Het
Kdm5b	A	G	1: 134,555,067 (GRCm39)	Y1325C	possibly damaging	Het
Kif1a	T	C	1: 93,004,014 (GRCm39)	D156G	probably damaging	Het
Lrp1b	A	G	2: 40,692,996 (GRCm39)	F3401L	possibly damaging	Het
Lrrc63	A	G	14: 75,335,731 (GRCm39)	Y460H	probably damaging	Het
Myo15b	G	T	11: 115,762,282 (GRCm39)	E1201*	probably null	Het
Ndor1	A	T	2: 25,140,162 (GRCm39)	I84K	probably damaging	Het
Ndst4	T	A	3: 125,476,819 (GRCm39)	Y15N	probably damaging	Het
Or51af1	T	C	7: 103,141,840 (GRCm39)	T82A	probably benign	Het
Or5ar1	A	G	2: 85,671,725 (GRCm39)	S137P	probably damaging	Het
Pik3r6	T	A	11: 68,424,347 (GRCm39)	D317E	possibly damaging	Het
Ppp2r1a	G	A	17: 21,171,609 (GRCm39)	R28H	probably damaging	Het
Ptch1	T	G	13: 63,672,773 (GRCm39)	E944A	probably benign	Het
Ptpn12	G	A	5: 21,197,741 (GRCm39)	P700L	probably benign	Het
Setx	GTGGCT	GT	2: 29,044,073 (GRCm39)	1814	probably null	Het
Slc39a13	T	C	2: 90,895,247 (GRCm39)		probably null	Het
Smarca2	G	A	19: 26,661,327 (GRCm39)		probably null	Het
Taok1	A	T	11: 77,450,659 (GRCm39)	L371H	possibly damaging	Het
Treml4	G	A	17: 48,571,837 (GRCm39)	R80Q	probably benign	Het
Trim9	G	A	12: 70,393,126 (GRCm39)	H273Y	probably damaging	Het
Tyr	A	G	7: 87,087,148 (GRCm39)	S455P	probably benign	Het
Vmn1r214	G	A	13: 23,219,520 (GRCm39)	C338Y	probably benign	Het
Vmn2r52	G	A	7: 9,904,603 (GRCm39)	T412I	probably benign	Het
Wdr70	A	T	15: 8,108,698 (GRCm39)	C149*	probably null	Het
Wdr93	T	A	7: 79,418,159 (GRCm39)	V294E	possibly damaging	Het

Other mutations in Tas2r116

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01305:Tas2r116	APN	6	132,832,406 (GRCm39)	missense	probably benign	0.02
IGL01656:Tas2r116	APN	6	132,832,396 (GRCm39)	unclassified	probably benign
IGL01970:Tas2r116	APN	6	132,832,632 (GRCm39)	missense	probably benign	0.07
ANU22:Tas2r116	UTSW	6	132,832,406 (GRCm39)	missense	probably benign	0.02
R0490:Tas2r116	UTSW	6	132,832,984 (GRCm39)	missense	probably benign	0.02
R2422:Tas2r116	UTSW	6	132,832,557 (GRCm39)	missense	possibly damaging	0.88
R4516:Tas2r116	UTSW	6	132,833,113 (GRCm39)	missense	probably damaging	1.00
R4745:Tas2r116	UTSW	6	132,832,668 (GRCm39)	missense	probably benign	0.14
R4842:Tas2r116	UTSW	6	132,832,660 (GRCm39)	missense	probably benign	0.00
R7723:Tas2r116	UTSW	6	132,832,867 (GRCm39)	missense	probably benign	0.25
R8439:Tas2r116	UTSW	6	132,832,540 (GRCm39)	missense	probably damaging	0.99
R8551:Tas2r116	UTSW	6	132,832,993 (GRCm39)	missense	probably benign	0.01
R9009:Tas2r116	UTSW	6	132,832,963 (GRCm39)	missense	probably damaging	1.00
R9573:Tas2r116	UTSW	6	132,833,140 (GRCm39)	missense	probably benign	0.04
Z1088:Tas2r116	UTSW	6	132,832,911 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CAGAGATGCCAGCACTAAGG -3'
(R):5'- AAGCCATATGTGTAACCATATTCCCTG -3'

Sequencing Primer
(F):5'- TAAGGCCCACATCAGAGCCTTG -3'
(R):5'- CTACAAAGACTAGAAGATGCATAGAC -3'

Posted On

2015-04-29