Mutagenetix > Incidental Mutation

Incidental Mutation 'R0386:Or10aa3'

31273

Institutional Source

Beutler Lab

Gene Symbol

Or10aa3

Ensembl Gene

ENSMUSG00000047048

Gene Name

olfactory receptor family 10 subfamily AA member 3

Synonyms

GA_x6K02T2P20D-21124681-21123743, MOR123-2, Olfr432

MMRRC Submission

038592-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.057) question?

Stock #

R0386 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

173877941-173878879 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 173877965 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 9 (T9S)

Ref Sequence

ENSEMBL: ENSMUSP00000150596 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000062665] [ENSMUST00000213211] [ENSMUST00000213381]

AlphaFold

E9Q8M2

Predicted Effect

probably benign
Transcript: ENSMUST00000062665
AA Change: T9S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000060341
Gene: ENSMUSG00000047048
AA Change: T9S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	306	3.7e-47	PFAM
Pfam:7TM_GPCR_Srsx	35	307	1.2e-7	PFAM
Pfam:7tm_1	41	289	6.5e-21	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000192435

Predicted Effect

probably benign
Transcript: ENSMUST00000213211
AA Change: T9S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

Predicted Effect

probably benign
Transcript: ENSMUST00000213381
AA Change: T9S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

Coding Region Coverage

1x: 99.1%
3x: 98.1%
10x: 95.5%
20x: 90.3%

Validation Efficiency

98% (56/57)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aasdh	A	G	5: 77,044,308 (GRCm39)	V194A	probably damaging	Het
Adamts13	G	A	2: 26,876,691 (GRCm39)		probably null	Het
Ahnak	T	C	19: 8,988,508 (GRCm39)	M3264T	possibly damaging	Het
Birc6	T	A	17: 74,906,335 (GRCm39)	C1409S	probably damaging	Het
Camta1	C	A	4: 151,159,597 (GRCm39)	R1614L	probably damaging	Het
Dnah2	A	G	11: 69,338,687 (GRCm39)	V3161A	probably damaging	Het
Dnah5	A	T	15: 28,383,727 (GRCm39)	Y2983F	probably damaging	Het
Dnah6	G	A	6: 73,060,107 (GRCm39)	L2774F	probably damaging	Het
Dst	A	T	1: 34,256,917 (GRCm39)	T4398S	probably damaging	Het
Efcab5	G	A	11: 77,031,749 (GRCm39)	R42W	probably damaging	Het
Efcab5	A	T	11: 77,063,204 (GRCm39)	M96K	probably benign	Het
Elavl4	A	G	4: 110,063,902 (GRCm39)		probably benign	Het
Flt4	G	A	11: 49,535,213 (GRCm39)	A1214T	probably benign	Het
Fn1	G	T	1: 71,634,945 (GRCm39)	T2127N	probably damaging	Het
Foxj1	A	T	11: 116,222,629 (GRCm39)	S391R	possibly damaging	Het
Gabrb1	A	T	5: 72,266,150 (GRCm39)	Y269F	probably damaging	Het
Ghitm	A	G	14: 36,847,868 (GRCm39)	S259P	possibly damaging	Het
Gm16332	A	G	1: 139,851,928 (GRCm39)		noncoding transcript	Het
Gm16380	T	A	9: 53,791,727 (GRCm39)		noncoding transcript	Het
Gm9869	A	T	9: 60,745,344 (GRCm39)		probably benign	Het
Gm9936	G	A	5: 114,995,192 (GRCm39)	Q142*	probably null	Het
Hmbs	T	C	9: 44,248,305 (GRCm39)	Y260C	probably benign	Het
Hoxc5	T	A	15: 102,923,784 (GRCm39)	C193*	probably null	Het
Idh2	C	T	7: 79,748,005 (GRCm39)	A232T	probably damaging	Het
Lce1j	T	C	3: 92,696,695 (GRCm39)	K28E	unknown	Het
Lpgat1	C	T	1: 191,451,460 (GRCm39)		probably benign	Het
Lyst	T	C	13: 13,882,799 (GRCm39)		probably benign	Het
Megf11	A	G	9: 64,547,360 (GRCm39)	N235D	probably damaging	Het
Mst1r	T	A	9: 107,794,003 (GRCm39)		probably null	Het
Nr2c2ap	A	G	8: 70,584,237 (GRCm39)	D9G	probably benign	Het
Obscn	T	C	11: 59,027,165 (GRCm39)	T13A	probably damaging	Het
Ofcc1	A	C	13: 40,367,950 (GRCm39)	L188*	probably null	Het
Oma1	A	T	4: 103,182,398 (GRCm39)		probably benign	Het
Or5m11	A	G	2: 85,782,217 (GRCm39)	E270G	probably damaging	Het
Pcm1	T	C	8: 41,769,060 (GRCm39)	F1642S	probably damaging	Het
Pglyrp2	A	G	17: 32,639,836 (GRCm39)	M1T	probably null	Het
Pnpla5	G	T	15: 84,004,920 (GRCm39)	L144M	probably damaging	Het
Prdm10	C	A	9: 31,227,596 (GRCm39)	T67K	probably damaging	Het
Ralgapa1	A	T	12: 55,754,852 (GRCm39)	H1193Q	probably benign	Het
Sall1	A	G	8: 89,759,232 (GRCm39)	S291P	probably damaging	Het
Sdk2	T	C	11: 113,784,290 (GRCm39)	T150A	probably damaging	Het
Sel1l2	T	A	2: 140,117,361 (GRCm39)	Y170F	probably benign	Het
Sema4a	C	T	3: 88,344,107 (GRCm39)	V715I	possibly damaging	Het
Smgc	G	A	15: 91,738,841 (GRCm39)	A500T	probably benign	Het
Spef2	A	G	15: 9,584,148 (GRCm39)	V1639A	probably damaging	Het
Srrm4	A	G	5: 116,620,437 (GRCm39)		probably benign	Het
Tbc1d23	G	A	16: 57,009,636 (GRCm39)	H418Y	probably damaging	Het
Tbk1	A	G	10: 121,420,159 (GRCm39)	L10P	probably damaging	Het
Thumpd3	G	A	6: 113,042,621 (GRCm39)		probably null	Het
Trp53bp1	G	T	2: 121,035,424 (GRCm39)	T1609K	probably damaging	Het
Tut1	A	G	19: 8,932,919 (GRCm39)	N84S	probably benign	Het
Urb1	C	T	16: 90,593,287 (GRCm39)	G282R	probably damaging	Het
Usp19	A	T	9: 108,376,910 (GRCm39)	D1160V	probably damaging	Het
Usp9y	A	G	Y: 1,316,933 (GRCm39)	V1872A	probably damaging	Het
Zfp276	C	A	8: 123,986,242 (GRCm39)	Y386*	probably null	Het

Other mutations in Or10aa3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01025:Or10aa3	APN	1	173,878,251 (GRCm39)	missense	probably damaging	1.00
IGL03002:Or10aa3	APN	1	173,878,191 (GRCm39)	missense	probably benign	0.03
R0020:Or10aa3	UTSW	1	173,878,413 (GRCm39)	missense	probably damaging	0.99
R1735:Or10aa3	UTSW	1	173,878,365 (GRCm39)	missense	probably benign
R1932:Or10aa3	UTSW	1	173,878,244 (GRCm39)	missense	probably damaging	1.00
R2363:Or10aa3	UTSW	1	173,878,814 (GRCm39)	missense	probably damaging	1.00
R3930:Or10aa3	UTSW	1	173,878,076 (GRCm39)	missense	probably damaging	1.00
R4024:Or10aa3	UTSW	1	173,878,683 (GRCm39)	missense	probably benign	0.00
R4777:Or10aa3	UTSW	1	173,878,244 (GRCm39)	missense	probably damaging	1.00
R4946:Or10aa3	UTSW	1	173,878,400 (GRCm39)	missense	possibly damaging	0.95
R5250:Or10aa3	UTSW	1	173,878,838 (GRCm39)	missense	probably benign
R5646:Or10aa3	UTSW	1	173,878,853 (GRCm39)	nonsense	probably null
R6178:Or10aa3	UTSW	1	173,878,533 (GRCm39)	missense	probably benign	0.00
R6634:Or10aa3	UTSW	1	173,878,535 (GRCm39)	missense	probably benign	0.11
R7578:Or10aa3	UTSW	1	173,878,266 (GRCm39)	missense	possibly damaging	0.71
R7653:Or10aa3	UTSW	1	173,878,488 (GRCm39)	missense	probably benign	0.36
R8110:Or10aa3	UTSW	1	173,878,091 (GRCm39)	missense	probably benign	0.01
R8426:Or10aa3	UTSW	1	173,878,146 (GRCm39)	missense	probably damaging	1.00
R9008:Or10aa3	UTSW	1	173,878,413 (GRCm39)	missense	probably damaging	0.99
R9408:Or10aa3	UTSW	1	173,878,329 (GRCm39)	missense
RF014:Or10aa3	UTSW	1	173,878,553 (GRCm39)	missense	possibly damaging	0.68

Predicted Primers

PCR Primer
(F):5'- CCTGACTGCTGCCATAGTCTACAAATG -3'
(R):5'- TGCTGACATACCAGAGTTCCAGGAAG -3'

Sequencing Primer
(F):5'- CTGCCATAGTCTACAAATGTTGTTC -3'
(R):5'- TACCAGAGTTCCAGGAAGGAGAG -3'

Posted On

2013-04-24